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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAG-Myc-mG alpha i2

Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i2, myc-tag.

Catalog number RDB15368
Resource name pCAG-Myc-mG alpha i2
Clone info. Expression vector of mouse heterotrimeric G-protein alpha-subunit, G-alpha-i2, tagged with Myc at N-terminal, CAG promoter.
Comment The CMV promoter region of pcDNA3 (Clontech) was replaced with the promoter/enhancer region of pCAGGS (provided by Dr J.Miyazaki) to generate pcCAG. Expression was confirmed by the depositor with Western blotting.
Vector backbone pCAG-Myc (plasmid)
Selectable markers Amp^r (E. coli) Neo^r (mammalian cells).
Gene/insert name mouse Gnai2 cDNA
Depositor|Developer Nagata, Koh-ichi |
Other clones in our bank
External Database
mouse Gnai2
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
          Ordering Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Hamada, N. et al., J. Neurochem. 140 (1): 82-95, 2017)。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15368 pCAG-Myc-mG alpha i2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15368_A7I5p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pCAG-F_V3
          Sequence file: RDB15368_A7I5a.seq check
          >D04868A1-1_A7I5_pCAG-F_V3_A01_01_ABI24.ab1
              1 CCTGGGGGCC AGGCTCCTTC GGGGGGGAGG GGCAGGGCGG GGTTCGGCTT CTGGCGTGTG
             61 ACCGGCGGCT CTAGAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT CCTACAGCTC
            121 CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT TAATACGACT
            181 CACTATAGGG AGACCCAAGC TAATCGATAT GGAGCAGAAG CTGATCTCCG AGGAGGACCT
            241 GATATCAAGC TTTGGCTGCA CCGTGAGCGC CGAGGACAAG GCGGCAGCCG AGCGCTCTAA
            301 AATGATCGAC AAGAACCTGC GGGAGGACGG CGAGAAGGCG GCGCGGGAGG TGAAGTTGCT
            361 TCTGTTAGGT GCTGGAGAAT CAGGGAAGAG CACCATCGTC AAGCAGATGA AGATCATCCA
            421 TGAAGATGGC TACTCAGAAG AGGAGTGCCG GCAGTACCGT GCCGTGGTCT ACAGCAACAC
            481 CATCCAGTCT ATCATGGCCA TCGTGAAGGC CATGGGCAAC CTGCAGATCG ACTTTGCTGA
            541 CCCCCAGCGT GCGGATGATG CCAGGCAGCT GTTCGCCCTG TCCTGTGCTG CAGAGGAACA
            601 AGGGATGCTT CCTGAAGACC TGTCCGGTGT CATCCGGAGG CTCTGGGCTG ACCACGGTGT
            661 GCAAGCCTGC TTTGGCCGCT CACGAGAATA CCAGCTCAAT GACTCAGCCG CTTACTACCT
            721 GAATGATCTG GAGCGCATTG CACAGAGTGA CTACATCCCT ACACAGCACG ATGTGCTGCG
            781 GACCCGTGTG AAGACCACGG GCATCGTGGA AACACACTTC ACCTTCAAGG ACTTACACTT
            841 CAAGATGTTT GATGTGGGTG GTCAGCGGTC TGAGCGCAAG AAGTGGATCC ACTGCTTTGA
            901 GGCGTCACGG GCCATCATCT TCTGTGTCGC CTTGAGCGCC TATGACTTGG TGCTGGCTGA
            961 GGATGAGGAG ATGACGCATG CATGAGAGCA TGAAGCTGTT TGACAGCATC TGCAACAACA
           1021 GTGTTCCACA GACACTCAAT CATTCCTCTC TCAACAGAAG ACTGGTTGGA GAAAAGAATC
          1081 ACCCAGAGCT CCTGGACATC TGGATTCCTT GATTACACGG GGGCTCAACC AAGGTATA
          //
          Primer: pCA-bgter
          Sequence file: RDB15368_A7I5b.seq check
          >D04868A1-1_A715_ pCA-bgter_A03_03_ABI24.ab1
              1 AGCACTGAAG TCCATAATTT TTGGCAGAGG GAAAAGATCT CAGTGGTATT TGTGAGCCAG
             61 GGCATTGGCC ACACCAGCCA CCACCTTCTG ATAGGCAGCC TGCACCTGAG GAGTGAATTC
            121 ATACGATTTA GGTGACACTA TAGAATAGGG CCCTCTAGAT GCATGCTCGA GGTTTAAACG
            181 CGGCCGCGGT ACCGCTAGAT CAGAAGAGGC CACAGTCCTT CAGGTTGTTC TTGATGATGA
            241 CGTCAGTGAC GGCATCGAAC ACAAACTGCA CGTTCTTGGT GTCGGTGGCG CACGTGAAGT
            301 GCGTGTAGAT CTCCTTGGTG TCTTTGCGCT TATTTAGATC CTCAAACTTG CTCTGGATGT
            361 AGCTGGCTGC CTCGTCGTAC TTGTTGGCCC CCGTGTACTC AGGGAAACAG ATGGTCAGGG
            421 AGCTCTGTGT GATCTTTTCT TCAAACAGGT CCTTCTTGTT GAGGAAGAGG ATGATGGAGG
            481 TGTCTGTGAA CCACTTGTTG TTGCAGATGC TGTCAAACAG CTTCATGCTC TCATGCATGC
            541 GGTTCATCTC CTCATCCTCA GCCAGCACCA AGTCATAGGC GCTCAAGGCG ACACAGAAGA
            601 TGATGGCCGT GACGCCCTCA AAGCAGTGGA TCCACTTCTT GCGCTCAGAC CGCTGACCAC
            661 CCACATCAAA CATCTTGAAG TGTAAGTCCT TGAAGGTGAA GTGTGTTTCC ACGATGCCCG
            721 TGGTCTTCAC ACGGGTCCGC AGCACATCCT GCTGTGTAGG GATGTAGTCA CTCTGTGCAA
            781 TGCGCTCCAG ATCATTCAGG TAGTAAGCGG CTGAGTCATT GAGCTGGTAT TCTCGTGAGC
            841 GGCCAAAGCA GGCTTGCACA CCGTGGTCAG CCCAGAGCCT CCGGATGACA CCGGACAGGT
            901 CTTCAGGAAG CATCCCTTGT TCCTCTGCAG CACAGGACAG GGCGAACAGC TGCCTGGCAT
            961 CATCCGCACG CTGGGGGTCA GCAAAGTCGA TCTGCAGGTT GCCCATGGGC CTTCACGATG
           1021 GCCATGATAG ACTGGGATGG TTGTGCTGTA GACACGGCAC GGGTACTGCC GGCACCTCCT
           1081 CTTCTGAGTT AGCATCTTCA TGATGATCTC ATCTGGCTGG ACGAATGTTG CTCTTTCCCT
          1141 TGATTCCTCC AAG
          //
          Primer: SV40pro_F
          Sequence file: RDB15368_A7I5c.seq check
          >D04868A1-1_A7I5_SV40pro_F_C01_07_ABI24.ab1
              1 CCCTGGTCCT GGACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
             61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
            121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT
            601 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT
            661 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT
            721 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA
            781 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA
            841 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG
            901 CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG
            961 CCCAACCTGC CATCACGAGA TTTCGATCAC GCGCTTCTAT GAAAGGTGGG CTTCGGATCG
           1021 TTTTCGGGAC GGCGCCTGAT GATTCTCAGC GCGGGAATCT CATGCTGGAA GTCTCGCACT
          1081 GACTGTATGC AGCTATATGC TACGATTTAA GGCCAGTAC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Hamada, N., Role of a heterotrimeric G-protein, Gi2, in the corticogenesis: possible involvement in periventricular nodular heterotopia and intellectual disability. J. Neurochem. 140 (1): 82-95 (2017). PMID 27787898.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl