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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3/hArf6(WT)-HA

Expression vector of human ARF6, wild type.

Catalog number RDB15349
Resource name pcDNA3/hArf6(WT)-HA
Alternative name W42
Clone info. Expression vector of human ADP-ribosylation factor6 (ARF6), wild type, tagged with HA epitope at C-ter.
Comment Expression was confirmed by the depositor with immunostaining.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cels).
Gene/insert name human ARF6 cDNA
Depositor|Developer Nakayama, Kazuhisa |
Other clones in our bank
External Database
human ARF6
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012). RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makyio, H. et al. EMBO. J., 31 (11): 2590-2603, 2012)。本件リソースは非営利機関にのみ提供し、利用範囲は学術研究に制限する。
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          RDB15349 pcDNA3/hArf6(WT)-HA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3/hArf6(WT)-HA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15349).

          Reference section:

          Makyio, H., Ohgi, M., Takei, T., Takahashi, S., Takatsu, H., Katoh, Y., Hanai, A., Ueda, T., Kanaho, Y., Xie, Y., Shin, H.W., Kamikubo, H., Kataoka, M., Kawasaki, M., Kato, R., Wakatsuki, S., Nakayama, K., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15349_A7I5p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro, insert (hArf6(WT)), HA
          Sequence file: RDB15349_A7I5a.seq check
          >D03588B4_A7I5_2_CMV_Forward_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GCNNNGGGCG GTANGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTTGGTACC GAGCTCGGAT CTGCCACCAT GGGGAAGGTG
            181 CTATCCAAAA TCTTCGGGAA CAAGGAAATG CGGATCCTCA TGTTGGGCCT GGACGCGGCC
            241 GGCAAGACAA CAATCCTGTA CAAGTTGAAG CTGGGCCAGT CGGTGACCAC CATTCCCACT
            301 GTGGGTTTCA ACGTGGAGAC GGTGACTTAC AAAAATGTCA AGTTCAACGT ATGGGATGTG
            361 GGCGGCCAGG ACAAGATCCG GCCGCTCTGG CGGCATTACT ACACTGGGAC CCAAGGTCTC
            421 ATCTTCGTAG TGGACTGCGC CGACCGCGAC CGCATCGATG AGGCTCGCCA GGAGCTGCAC
            481 CGCATTATCA ATGACCGGGA GATGAGGGAC GCCATAATCC TCATCTTCGC CAACAAGCAG
            541 GACCTGCCCG ATGCCATGAA ACCCCACGAG ATCCAGGAGA AACTGGGCCT GACCCGGATT
            601 CGGGACAGGA ACTGGTATGT GCAGCCCTCC TGTGCCACCT CAGGGGACGG ACTCTATGAG
            661 GGGCTCACAT GGTTAACCTC TAACTACAAA TCTCTCGAGT ATCCATACGA TGTACCAGAC
            721 TACGCAGANA TCCACTAGTT CTAGAGGGCC CTATTCTATA GTGTCACCTA AATGCTAGAG
            781 CTCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC
            841 CCCNNGCCTT CCTTGACCCT NGAANGTGCC ACTNCNNCTN NNCTTTCCTA ATAAAATGAN
          901 GAAATTGCAT CGNNNNNNCT NNNNNNNNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: NeoR
          Sequence file: RDB15349_A7I5b.seq check
          >D03588B4-A7I5_2_SV40pro_F_E01_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTNGTGA NGANGNTTTT TTGGAGGNCT AGGCTTTTGC
             61 AAAAAGCTCC CGNNNNGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG
            121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA
            181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT
            241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT
            301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG
            361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT
            421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC
            481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC
            541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA
            601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG
            661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT
            721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG
            781 CTATCANGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA NAGCTTGGCG GCGANNGNGC
            841 TGACCGCTTC CTCNTGCTTT ACGGNATCGC CNCTCCCGNN NCGCAGCGCA TCGCCTTCTA
            901 TCGCCTTCTT GNCNANTNCT NCTGANCGGG NNTCTGGGGN NNNNAAATGN NCNNNCNANC
          961 NANGCCNNNN CCTGNCNNNC NNNANANNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Makyio, H., Structural basis for Arf6-MKLP1 complex formation on the Flemming body responsible for cytokinesis. EMBO J. 31 (11): 2590-2603 (2012). PMID 22522702.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl