Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGL4-JF1_886-1146anti

Luciferase reporter of the mouse Ccnb1ip1, nt.886 to nt.1146, antisense orientation.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15334_A7Hmp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Region: insert 5' Sequence file: RDB15334_A7Hma.seq
>D04779A8_A7Hm_2_pGL4-4174F_B01_04_ABI24.ab1     1 AATGGGGTCA GAACATTTCT CTGGCCTAAC TGGCCAAATG TGCTGCCAGA CTGCAACTCA    61 ACGCTCATTT TAAGATTGAA CTCCTACACA AAATTTCTTT CAGGATCATG ACACAAAACA   121 AACAAACACA GATAATTGAA ACGGTTTGGG CATGTCCTTG TTCTATTCCA AACAGTTTCC   181 ATCCTGAGTA ACCTCCCGTC CTTTTGCAAC CTCAACATAG CTAACTTATG CAAGGTAATA   241 ATAGGTCCAA TTATAAACAG TCTGTAACTA TCCAACTTAG AAATTGCCTT CAGGGCGGCC   301 TCGGCGGCCA AGCTTAGACA CTAGAGGGTA TATAATGGAA GCTCGACTTC CAGCTTGGCA   361 ATCCGGTACT GTTGGTAAAG CCACCATGGA AGATGCCAAA AACATTAAGA AGGGCCCAGC   421 GCCATTCTAC CCACTCGAAG ACGGGACCGC CGGCGAGCAG CTGCACAAAG CCATGAAGCG   481 CTACGCCCTG GTGCCCGGCA CCATCGCCTT TACCGACGCA CATATCGAGG TGGACATTAC   541 CTACGCCGAG TACTTCGAGA TGAGCGTTCG GCTGGCAGAA GCTATGAAGC GCTATGGGCT   601 GAATACAAAC CATCGGATCG TGGTGTGCAG CGAGAATAGC TTGCAGTTCT TCATGCCCGT   661 GTTGGGTGCC CTGTTCATCG GTGTGGCTGT GGCCCCAGCT AACGACATCT ACAACGAGCG   721 CGAGCTGCTG AACAGCATGG GCATCAGCCA GCCCACCGTC GTATTCGTGA GCAAGAAAGG   781 GCTGCAAAAG ATCCTCAACG TGCAAAAGAA GCTACCGATC ATACAAAAGA TCATCATCAT   841 GGATAGCAAG ACCGACTACC AGGGCTTCCA AAGCATGTAC ACCTTCGTGA CTTCCCATTT   901 GCCACCCGGC TTCAACGAGT ACGACTTCGT GCCCGAGAGC TTCGACCGGG ACAAAACCAT   961 CGCCCTGATC ATGAACAGTA GTGGCAGTAC CGGATTGCCC AAGGGCGTAG CCCTACCGCA  1021 ACCGGCACCG CTTGTGTCCG ATTCAGTTCA ATGCCCGCGA CCCCCATCTT TCGCAATAGA 1081 TTCATTCCCC CGACACCGCT ATCCCTCAGC CGGGTGGGTG TGC //
Primer: pGL4-136R Region: insert 3' Sequence file: RDB15334_A7Hmb.seq
>D04779A8_A7Hm_2_pGL4-136R_C01_07_ABI24.ab1     1 GTTTTTTGTT TTTGGCATCT TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTG    61 GAAGTCGAGC TTCCATTATA TACCCTCTAG TGTCTAAGCT TGGCCGCCGA GGCCGCCCTG   121 AAGGCAATTT CTAAGTTGGA TAGTTACAGA CTGTTTATAA TTGGACCTAT TATTACCTTG   181 CATAAGTTAG CTATGTTGAG GTTGCAAAAG GACGGGAGGT TACTCAGGAT GGAAACTGTT   241 TGGAATAGAA CAAGGACATG CCCAAACCGT TTCAATTATC TGTGTTTGTT TGTTTTGTGT   301 CATGATCCTG AAAGAAATTT TGTGTAGGAG TTCAATCTTA AAATGAGCGT TGAGTTGCAG   361 TCTGGCAGCA CATTTGGCCA GTTAGGCCAG AGAAATGTTC TGGCACCTGC ACTTGCACTG   421 GGGACAGCCT ATTTTGCTAG TTTGTTTTGT TTCGTTTTGT TTTGATGGAG AGCGTATGTT   481 AGTACTATCG ATTCACACAA AAAACCAACA CACAGATGTA ATGAAAATAA AGATATTTTA   541 TTGCGGCCTG TCCAATACCT CCCGTACCTT AATATTACTT ACTTATCCTT GAGAGACGTA   601 CTAGTAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG ACGAGTATGA GCATCCAACA   661 TTTTCGTGTC GCACTCATTC CCTTCTTTGC GGCATTTTGC TTGCCTGTTT TTGCACACCC   721 CGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAACTG GGTGCAAGAG TGGGCTATAT   781 CGAACTGGAT CTCAATAGCG GCAAGATCCT TGAGTCTTTC CGCCCCCGAA GAACGATTCC   841 CGATGATGAG CACTTTCAAA GTACTGCTAT GTGGCGCGGT GTTGTCCCGT ATAGACGCCG   901 GGCAAGAGCA GCTTGGTCGC CGTATACACT ACTCACAAAA CGACTTGGTT TGAGTACTCG   961 CCCGGTCACG GGAAAAAGCA TCTTTACGGA TGGGCATGAC GGTAAGAGAA TTTGTGTAGT  1021 GCCTGCCCAT TTACCCATGA GCGGACACAA CCGCGGTGAA CCTTTACGTT CCTGACACGA 1081 TTCCGAAGAC CGTAAAGGAA GCTTGACTGC CATTTTCCTT TCC //
Primer: pAxCALNL_F1 Region: Luciferase 3' Sequence file: RDB15334_A7Hmc.seq
>D04779A8_A7Hm_2_pAxCALNLF1_D01_10_ABI24.ab1     1 CAACAAAGGA TATTCTTATC AATGTCTGCT CGAAGCGGCC GGCCGCCCCG ACTCTAGAAT    61 TATTACACGG CGATCTTGCC GCCCTTCTTG GCCTTAATGA GAATCTCGCG GATCTTGCGG   121 GCGTCCAACT TGCCGGTCAG TCCTTTAGGC ACCTCGTCCA CGAACACAAC ACCACCGCGC   181 AGCTTCTTGG CGGTTGTAAC CTGGCTGGCC ACATAGTCCA CGATCTCCTT CTCGGTCATG   241 GTTTTACCGT GTTCCAGCAC GACGACTGCG GCGGGCAGCT CGCCGGCATC GTCGTCGGGC   301 AGGCCGGCGA CCCCGGCGTC GAAGATGTTG GGGTGTTGCA GCAGGATGCT CTCCAGTTCG   361 GCTGGGGCTA CCTGGTAGCC CTTGTATTTG ATCAGGCTCT TCAGCCGGTC CACGATGAAG   421 AAGTGCTCGT CCTCGTCCCA GTAGGCGATG TCGCCGCTGT GCAGCCAGCC GTCCTTGTCG   481 ATGAGAGCGT TTGTAGCCTC GGGGTTGTTA ACGTAGCCGC TCATGATCAT GGGGCCACGG   541 ACGCACAGCT CGCCGCGCTG GTTCACACCC AGTGTCTTAC CGGTGTCCAA GTCCACCACC   601 TTAGCCTCGA AGAAGGGCAC CACCTTGCCT ACTGCGCCAG GCTTGTCGTC CCCTTCGGGG   661 GTGATCAGAA TGGCGCTGGT TGTTTCTGTC AGGCCGTAGC CCTGGCGGAT GCCTGGTAGG   721 TGGAAGCGTT TGGCCACGGC CTCACCTACC TCCTTGCTGA GCGGCGCCCC GCCGCTGGCG   781 ATCTCGTGCA AGTTGCTTAG GTCGTACTTG TCGATGAGAG TGCTCTTAGC GAAGAAGCTA   841 AATAGTGTGG GCACCCAGCA GGGCAGATTG AATCTTTATA GTCTTGGCAA GCCTGCGCAA   901 GAATAGCTCC TTCCTCGAAA GCGGTACAAT GAAGCACGAA CCCCGAAGCC CGCCAGATCC   961 AAGTAAGCCC CAAGCCGGTT GGTGAAACAA TGCCCGGAAG CCGTTGATGA AATGGGCCAC  1021 CCACGCCTGA AGGATAAGCC GGTGGTCGGG GGAATGGATT CCTGGGTTTG CACGAAAGAA  1081 ATGGGGGTTC GCCCGGGCAA TGAACCTTGA AATTCCGGGA CACACCAAGA CCGGGGTGGC 1141 GACGTATTGC CGCGGGGTAA G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl