Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGL4-JF1_886-1349anti

Luciferase reporter of the mouse Ccnb1ip1, nt.886 to nt.1349, antisense orientation.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015).
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yoshida, W. et al. PLoS One, 10 (6): e0131204, 2015)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15330_A7Hmp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Region: insert 5' Sequence file: RDB15330_A7Hma.seq
>D04779A4_A7Hm_1_pGL4-4174F_D04_10_ABI24.ab1     1 GCGGGGTCAG ACATTTCTCT GGCTACTGGC CCAAGGACGG GAAGCTACCT AAGTAATGGG    61 CCTCTGAACC TGATTGGCTG AGTTCAAGAT CTCCTCCTCC TCCTCCTCCT CCTCCTCCTC   121 CTCCTCCTCC TCCTCTTCTT CCTCTTCCTC CTCCTCTTCT TCCTCTTCCT CCTCCTCTTC   181 TTCTTTTTCT TCCTCCTTCT TCTCCCCCTC CTCCCTGTTC CCCTCTTAAT TTTAAAATGT   241 GCTGCCAGAC TGCAACTCAA CGCTCATTTT AAGATTGAAC TCCTACACAA AATTTCTTTC   301 AGGATCATGA CACAAAACAA ACAAACACAG ATAATTGAAA CGGTTTGGGC ATGTCCTTGT   361 TCTATTCCAA ACAGTTTCCA TCCTGAGTAA CCTCCCGTCC TTTTGCAACC TCAACATAGC   421 TAACTTATGC AAGGTAATAA TAGGTCCAAT TATAAACAGT CTGTAACTAT CCAACTTAGA   481 AATTGCCTTC AGGGCGGCCT CGGCGGCCAA GCTTAGACAC TAGAGGGTAT ATAATGGAAG   541 CTCGACTTCC AGCTTGGCAA TCCGGTACTG TTGGTAAAGC CACCATGGAA GATGCCAAAA   601 ACATTAAGAA GGGCCCAGCG CCATTCTACC CACTCGAAGA CGGGACCGCC GGCGAGCAGC   661 TGCACAAAGC CATGAAGCGC TACGCCCTGG TGCCCGGCAC CATCGCCTTT ACCGACGCAC   721 ATATCGAGGT GGACATTACC TACGCCGAGT ACTTCGAGAT GAGCGTTCGG CTGGCAGAAG   781 CTATGAAGCG CTATGGGCTG AATACAAACC ATCGGATCGT GGTGTGCAGC GAGAATAGCT   841 TGCAGTTCTT CATGCCCGTG TTGGGTGCCC TGTTCATCGG TGTGGCTGTG GCCCCAGCTA   901 ACGACATCTA CAACGAGCGC GAGCTGCTGA ACAGCATGGG CATCAGCCAG CCCACCGGTC   961 GTATTCGTGG AGCAAGAAAG GGCTGCAAAA AGATCCTCAC GTGCAAAAGA AGCTACCGAT  1021 CATACAAAGA TCATCATCAT GGGATAGCAA GGAACGGACT AACCAAGGGC TTCCGAAGGC 1081 ATGGTACACC TTCGATGGAC CTTCCCGATT TGGCCCACCC CCGTGC //
Primer: pGL4-136R Region: insert 3' Sequence file: RDB15330_A7Hmb.seq
>D04779A4_A7Hm_1_pGL4-136R_D05_11_ABI24.ab1     1 GCCTTATGCT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTGG    61 AAGTCGAGCT TCCATTATAT ACCCTCTAGT GTCTAAGCTT GGCCGCCGAG GCCGCCCTGA   121 AGGCAATTTC TAAGTTGGAT AGTTACAGAC TGTTTATAAT TGGACCTATT ATTACCTTGC   181 ATAAGTTAGC TATGTTGAGG TTGCAAAAGG ACGGGAGGTT ACTCAGGATG GAAACTGTTT   241 GGAATAGAAC AAGGACATGC CCAAACCGTT TCAATTATCT GTGTTTGTTT GTTTTGTGTC   301 ATGATCCTGA AAGAAATTTT GTGTAGGAGT TCAATCTTAA AATGAGCGTT GAGTTGCAGT   361 CTGGCAGCAC ATTTTAAAAT TAAGAGGGGA ACAGGGAGGA GGGGGAGAAG AAGGAGGAAG   421 AAAAAGAAGA AGAGGAGGAG GAAGAGGAAG AAGAGGAGGA GGAAGAGGAA GAAGAGGAGG   481 AGGAGGAGGA GGAGGAGGAG GAGGAGGAGG AGGAGGAGAT CTTGAACTCA GCCAATCAGG   541 TTCAGAGGCC CATTACTTAG GTAGCTTCCC GTCCTTGGGC CAGTTAGGCC AGAGAAATGT   601 TCTGGCACCT GCACTTGCAC TGGGGACAGC CTATTTTGCT AGTTTGTTTT GTTTCGTTTT   661 GTTTTGATGG AGAGCGTATG TTAGTACTAT CGATTCACAC AAAAAACCAA CACACAGATG   721 TAATGAAAAT AAAGATATTT TATTGCGGCC TGTCCAATAC CTCCCGTACC TTAATATTAC   781 TTACTTATCC TTGAGAGACG TACTAGTAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA   841 GGACGAGTAT GAGCATCCAA CATTTTTCGT GTCGCACTCA TTCCCTTCTT TTGCGGCATT   901 TTGCTTGCCC TGTTTTTGCA CACCCCGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC   961 AACTGGGTTG CAAGAGTGGG CTATATCGAA CTGGATCTCA ATTAGCGGCA AGATCCCTTT  1021 GAGTTCTTTC CGCCCCTCGA AGAAACCGAT TCCCGGATGG ATGGAAGCAA CTTTCCAAAG 1081 GTACCTGGCC TAATGTGGCC GCGATGTTTG ATTCCCCGGT ATAG //
Primer: pAxCALNL_F1 Region: Luciferase 3' Sequence file: RDB15330_A7Hmc.seq
>D04779A4_A7Hm_1_pAxCALNLF1_H10_22_ABI24.ab1     1 TAAATGTATC TTATCATGTC TGCTCGAGCG GCCGGCCGCC CCGACTCTAG AATTATTACA    61 CGGCGATCTT GCCGCCCTTC TTGGCCTTAA TGAGAATCTC GCGGATCTTG CGGGCGTCCA   121 ACTTGCCGGT CAGTCCTTTA GGCACCTCGT CCACGAACAC AACACCACCG CGCAGCTTCT   181 TGGCGGTTGT AACCTGGCTG GCCACATAGT CCACGATCTC CTTCTCGGTC ATGGTTTTAC   241 CGTGTTCCAG CACGACGACT GCGGCGGGCA GCTCGCCGGC ATCGTCGTCG GGCAGGCCGG   301 CGACCCCGGC GTCGAAGATG TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGCTGGGG   361 CTACCTGGTA GCCCTTGTAT TTGATCAGGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT   421 CGTCCTCGTC CCAGTAGGCG ATGTCGCCGC TGTGCAGCCA GCCGTCCTTG TCGATGAGAG   481 CGTTTGTAGC CTCGGGGTTG TTAACGTAGC CGCTCATGAT CATGGGGCCA CGGACGCACA   541 GCTCGCCGCG CTGGTTCACA CCCAGTGTCT TACCGGTGTC CAAGTCCACC ACCTTAGCCT   601 CGAAGAAGGG CACCACCTTG CCTACTGCGC CAGGCTTGTC GTCCCCTTCG GGGGTGATCA   661 GAATGGCGCT GGTTGTTTCT GTCAGGCCGT AGCCCTGGCG GATGCCTGGT AGGTGGAAGC   721 GTTTGGCCAC GGCCTCACCT ACCTCCTTGC TGAGCGGCGC CCCGCCGCTG GCGATCTCGT   781 GCAAGTTGCT TAGGTCGTAC TTGTCGATGA GAGTGCTCTT AGCGAAGAAG CTAAATAGTG   841 TGGGCACCAG CAGGGCAGAT TGAATCTTAT AGTCTTGCAA GCTGCGCAAG AATAGCTCCT   901 CCTCGAAGCG GTACATGAGC ACGACCCGAA AGCCGCAGAT CAAGTAGCCC AGCGTGGTGA   961 ACATGCCGAA AGCCGTGTGA AATGGCACCA CGCTGAGGAT AGCGTGTCGG GATGATCTGG  1021 CTGCCGGAGA TGGGGTCGCG GCATGACTGA TTCGACACAA GCGTGCGGTT CGTAGGCTAC 1081 GCCCTGGTCA ATTCAGTTAC TGCCCAACTA AC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl