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pEGFP-C1-mouse Ccz1

Expression vector of mouse CCZ1 vacuolar protein trafficking and biogenesis associated (Ccz1).

Catalog number RDB15295
Resource name pEGFP-C1-mouse Ccz1
Clone info. Expression vector of mouse CCZ1 vacuolar protein trafficking and biogenesis associated (Ccz1), fused with N-terminal EGFP, CMV promoter. Recruited to the late-endosomal state of macropinosomes (Fig.5 of Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2011)..
Comment Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone pEGFP-C1 (plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name mouse Ccz1 cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse Ccz1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2016).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yasuda, S. et al., J. Cell Sci., 129 (2), 329-340, 2016)。
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          RDB15295 pEGFP-C1-mouse Ccz1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Yasuda, S., Mon1-Ccz1 activates Rab7 only on late endosomes and dissociates from the lysosome in mammalian cells. J. Cell Sci. 129 (2): 329-340 (2016). PMID 26627821.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15295_A7Gop1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EGFP-C2 (Pr0402)
          Region: EGFP 3',insert 5'
          Sequence file: RDB15295_A7Goa.seq check
          >D04773B4_A7Go_2_EGFP-C2_H12_24_ABI24.ab1
              1 TTATACCCAA CGAGAGCGCG ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT
             61 CACTCTCGGC ATGGACGAGC TGTACAAGTC CGGACTCAGA TCCATGGCGG CAGCCGCGGC
            121 CGGGCCGGGT GCTTGGGCGG CCCAGGAGAA GCAGTTCCCG CCAGCGCTGC TGAGCTTCTT
            181 CATCTACAAC CCCCGTTTCG GGCCGCGCGA GGGAGAGGAA GAAAATAAAA TTCTGTTTTA
            241 CCATCCAAAT GAAGTAGAAA AGAATGAAAA AATTAGAAAT GTTGGATTAT GTGAAGCAAT
            301 TGTACAGTTT ACAAGGACTT TTAGCCCATC GAAACCTGCA AAATCTTTAC ACACACAGAA
            361 GAACAGACAG TTCTTCAATG AACCAGAAGA AAATTTCTGG ATGGTTATGG TTGTTCGGAA
            421 TCCTATCATC GAGAAGCAAA GTAAAGATGG CAAGGCGGTA GTGGAGTACC AAGAGGAGGA
            481 GCTGTTGGAC AAGGTGTACA GCTCAGTGCT GCAGCAGTGC TACAGCATGT ACAAGCTTTT
            541 CAATGGGACG TTTCTGAAAG CCATGGAAGA TGGAGGCGTC AAGCTCCTAA AGGAAAGATT
            601 AGAGAAATTC TTCCATCGGT ATTTGCAGAC ACTACATTTG CAGTCATGTG ACCTACTTGA
            661 TATTTTTGGT GGAATCAGCT TCTTCCCATT GGATAAAATG ACTTACTTGA AAATCCAGTC
            721 CTTTATTAAC CGAATGGAAG AAAGCCTAAG TGTAGTCAAA TATACAGCAT TCCTCTACAA
            781 TGACCAACTT ATCTGGAGTG GACTAGAGCA AGATGACATG AGAATTTTGT ACAAATACCT
            841 CACCACCTCA CTGTTCCCCA GGCACATCGA ACCTGAGTTG GCGGGAAGGG ACTCCCCAGT
            901 CAGGGCAGAG ATGCCTGGGA ATCTTCAGCA CTATGGAAGA TTTCTTACTG GACCCTTGAA
            961 CCTTAATGAT CCAGAAGCAA AATGCAGATT CCCCAAAATT TTTGTAAATA CAGATGACAC
           1021 GTATGAGAGC TCCATTTAAT TGTTTATAGC CATGAGTGCA GCCGTGTGCT TCATGATGAC
           1081 GCTCACGCCG CTCACGCCTG AATTTCTGCC GAGACTGGAC AGCAATCGAT TGGGTCCCAA
          1141 GCCTTAACCG
          //
          Primer: pAxEF R2 (Pr0059)
          Region: SV40pA,insert 3'
          Sequence file: RDB15295_A7Gob.seq check
          >D04773B4_A7Go_2_pAxEF_R2_F12_18_ABI24.ab1
              1 TGGGTCAAAA TGCTTTATTT GTGAATTTGT GATGCTATTG CTTTATTTGT AACCATTATA
             61 AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT TTATGTTTCA GGTTCAGGGG
            121 GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT GGCTGATTAT
            181 GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT GCAGGCGGCC
            241 GCGAATTCAC TAGTGATTTC AATCCAAGAA GAAGATGTTG TTGAACTGCG TGGCACACAG
            301 TTTCTTCACC TCTTCATTGA CCTCGATCAG GTTCGCATTC TTCTGACTGA GAATCACGTA
            361 CAGCTCCCGC TGGTCAGACT TCTTCCCAAC AACCCAGTAG TCGCTCATGG CCTTCACAAT
            421 GATTTCTTCA TCTTCATCTG CTCTGGTAAA GTCACTGTTG ATGTCCCCTA ATATCTTCAT
            481 TAGATCTGGG TGGACGGATG TGAGTGACAC ACTGGGCGTT TTCCTCATGT GAATTGTACT
            541 TTTCTCTGCT AAATTCATGT GGTTGAAGTA GATGAACTTA AACTGGGGCT CCTTTTCAGA
            601 CCCAGAGATC CTCTTGTTGA TATTAAACTG CTCACAGATG TCAGATGCCA GCACCGTAAG
            661 CTGGGGCCCA ACGATGCTGT CCAGTCTTCG GCAGAAATCC AGCGTGAGCG GCGTGGAGGC
            721 GTCAATCATG AAGCACACGG CTGCACTCAT GGCCTTATAA ACAATTAAAT GGAGCTCTTC
            781 ATACGTGTCA TCTGTATTTA CAAAAATTTT GGGGAATCTG CATTTTGCTT CTGGATCATT
            841 AAGTTCAAGG GTCCAGTAAG AAATCTTCCA TAGTGCTGAA GATTCCCAGG CATCTCTGCC
            901 CTGACTGGGG AGTCCCTTCC CGCCAACTCA GTTCGATGTG CCTGGGGAAC AGTGAGGTGG
            961 TGAGGTATTT GTACAAAATT CTCATGTCAT CTTGCTCTAG TCCACTCCAG ATAAGTTGGG
           1021 TCATTGTAGA GGATGCTGTA TATTTGACTA CACTTAGCTT TCTTCATTCG GTATAAGGAC
           1081 TGGATTTTCC AGTAGTCATT TTTATCATGG GAGAGGCTGA ATCATAAATA TCAAGTAAGT
          1141 TCACATGACT GCAATGTAAG TGTTCTTGCA AAATATAC
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,EGFP 5',insert 5'
          Sequence file: RDB15295_A7Goc.seq check
          >D04773B4_A7Go_2_CMV-Forward_G12_21_ABI24.ab1
              1 TGTTACAACT CCGCCCCATT GACGCAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TGGTTTAGTG AACCGTCAGA TCCGCTAGCG CTACCGGTCG CCACCATGGT
            121 GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA
            181 CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA
            241 GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT
            301 GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA
            361 CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA
            421 GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA
            481 CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT
            541 GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT
            601 CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA
            661 CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT
            721 GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT
            781 GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTCCGGACT
            841 CAGATCCATG GCGGCAGCCG CGGCCGGGCC GGGTGCTTGG GCGGCCCAGG AGAAGCAGTT
            901 CCCGCCAGCG CTGCTGAGCT TCTTCATCTA CAACCCCCGT TTCGGGCCGC GCGAGGGAGA
            961 GGAAGAAAAT AAAATTCTGT TTTACCATCC AAATGAAGTA GAAAAGAATG AAAAAAATTA
           1021 GAAATGTGGG ATTATGTGAA AGCAAATTGT ACAGTTTACA AGGAACTTTT AGCCCAATCG
           1081 GAATCTGCAA AATCTTTACA CACACAGAGG ACCAAACAGT CCTCAATGAC CAGAAAGAAA
          1141 AATTTCTTGG GAATGGGTTA TAGGGGATG
          //
          Primer: SV40pro_F_V2 (Pr0154)
          Region: NeoR_KanR
          Sequence file: RDB15295_A7God.seq check
          >D04773B4_A7Go_2_SV40pro_F_V2_H12_24_ABI24.ab1
              1 AACGAGCTTT TTGAGGCCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT
             61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
            121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
            181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
            241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
            301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
            361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
            421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
            481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
            541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC
            601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
            661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
            721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
            781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
            841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGAA ATGACCGACC AAGCGACGCC
            901 CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG
            961 ATCGTTTTCC GGGACGCCGG CTGGATGATC TCAGCGCGGG GATCTCATGC TGGAGTCTCG
           1021 CCCACCCTAG GGGAGGCTAC TGAACACGAT GAGACATACT GAGACCCGCG CTATGACGCA
          1081 TTAAAGGACG GATACGCACG TGATGATCGT GTCCATAACG CGGGGTTTCG GATCCAAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yasuda, S., Mon1-Ccz1 activates Rab7 only on late endosomes and dissociates from the lysosome in mammalian cells. J. Cell Sci. 129 (2): 329-340 (2016). PMID 26627821.

          2023.04.06

          GNP_filter3_RDBDEP_html_230405.pl