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mCherry-hGeminin(1/110) / pCSII-EF

Lentivirus vector plasmid of S/G2/M-R(N).

Catalog number RDB15269
Resource name mCherry-hGeminin(1/110) / pCSII-EF
Clone info. Lentivirus vector plasmid of S/G2/M-R(N). Fluorescence was detected in the nucleus.
Comment For the composition, refer Fig.1 of Sakaue-Sawano, A., Chem. Biol. 15 (12): 1243-1248 (2008). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name human Geminin cDNA Discosoma sp. RFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
human Geminin Discosoma sp. RFP

          Reference sequence
            
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
          Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
          Additional terms and conditions for distribution The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
          Ordering Please visit Ordering instruction.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano A, Ohtawa K, Hama H, Kawano M, Ogawa M, Miyawaki A. (2008) Tracing the Silhouette of Individual Cells in S/G2/M Phases with Fluorescence. Chemistry & Biology, 15: 1243-1248.
          Sakaue-Sawano A, Kobayashi T, Ohtawa K, Miyawaki A. (2011) Drug-induced cell cycle modulation leading to cell-cycle arrest, nuclear mis-segregation, or endoreplication. BMC Cell Biology, 12: 2.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          5. The RECIPIENT shall obtain written approval from the UCSD using the MTA provided by the Technology Transfer office of UCSD.
          付加的提供条件 レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 【追加提供依頼書】受入れ確認書(書式J)
          このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          提供条件は英文のまま記載してください。提供条件の言語は英語のみ。
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15269 mCherry-hGeminin(1/110) / pCSII-EF DNA solution

          Ordering instruction of plasmids [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15269_A7H3p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB15269_A7H3a.seq check
          >D04498H9-1_A7H3_EF1a-C_F_C01_07_ABI24.ab1
              1 TCATTGGAAT TGGCCCTTTT TGAGTTTGGA TCTTGGTTCA TTCTCAAGCC TCAGACAGTG
             61 GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAACAC GCTACCGGTC TCGAGAATTC
            121 GCCACCATGG TGAGCAAGGG CGAGGAGGAT AACATGGCCA TCATCAAGGA GTTCATGCGC
            181 TTCAAGGTGC ACATGGAGGG CTCCGTGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC
            241 GAGGGCCGCC CCTACGAGGG CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC
            301 CTGCCCTTCG CCTGGGACAT CCTGTCCCCT CAGTTCATGT ACGGCTCCAA GGCCTACGTG
            361 AAGCACCCCG CCGACATCCC CGACTACTTG AAGCTGTCCT TCCCCGAGGG CTTCAAGTGG
            421 GAGCGCGTGA TGAACTTCGA GGACGGCGGC GTGGTGACCG TGACCCAGGA CTCCTCCCTG
            481 CAGGACGGCG AGTTCATCTA CAAGGTGAAG CTGCGCGGCA CCAACTTCCC CTCCGACGGC
            541 CCCGTAATGC AGAAGAAGAC CATGGGCTGG GAGGCCTCCT CCGAGCGGAT GTACCCCGAG
            601 GACGGCGCCC TGAAGGGCGA GATCAAGCAG AGGCTGAAGC TGAAGGACGG CGGCCACTAC
            661 GACGCTGAGG TCAAGACCAC CTACAAGGCC AAGAAGCCCG TGCAGCTGCC CGGCGCCTAC
            721 AACGTCAACA TCAAGTTGGA CATCACCTCC CACAACGAGG ACTACACCAT CGTGGAACAG
            781 TACGAACGCG CCGAGGGCCG CCACTCCACC GGCGGCATGG ACGAGCTGTA CAAGGGATAT
            841 CCATCACACT GGCGGCCGCT CGAGATGAAT CCCAGTATGA AGCAGAAACA AGAAGAAATC
            901 AAAGAGAATA TAAAGAATAG TTCTGTCCCA AGAAGAACTC TGAAGATGAT TCAGCCTTCT
            961 GCATCTGGGA TCTCTTGTTG GAAGAGAAAA TGAGCTGTCC GCAGCTTGTC CAAAAGGAAA
           1021 CATCGGAATG ACCACTTTAA CATCTACAAC TTCCAGCCCT GGGGGTATGT CCCAGAATCT
           1081 AGTGAAAAAT AAAAAATCTG AGAGTCACCC AGGGAGTCAT TGGATCCTAA TGAATCAGAA
          1141 ATCAATCTCT CAGTATTGGA AAGGAAGTGG CAGAAAAAAA ACC
          //
          Primer: PRE_rev
          Sequence file: RDB15269_A7H3b.seq check
          >D04498H9-1_A7H3_PRE_rev_C02_08_ABI24.ab1
              1 ACGTTATCAC ATAGCGTAAA GGAGCAACAT AGTTAAGAAT ACCAGTCAAT CTTTCACAAA
             61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGATGTTAA CTCTAGATTA
            121 CAGCGCCTTT CTCCGTTTTT CTGCCACTTC CTTCCAATAC TGAGAGGATG GATTTTCTTT
            181 AATCATAAGA TCAAATGACT CCTGGGTGAC TCCTCCAAGA TTTTTATTTT CACTAGATTC
            241 TGGGACAATA ACCCCAGGGC TGGAAGTTGT AGATGTTAAG TGGTCATTCC GATGTTTCCT
            301 TTTGGACAAG CCTGCGGACA GCTCATTTTC TCTTCCAACA AGAGATCCAG ATGCAGAAGG
            361 CTGAATCATC TTCAGAGTTC TTCTTGGGAC AGAACTATTC TTTATATTCT CTTTGATTTC
            421 TTCTTGTTTC TGCTTCATAC TGGGATTCAT CTCGAGCGGC CGCCAGTGTG ATGGATATCC
            481 CTTGTACAGC TCGTCCATGC CGCCGGTGGA GTGGCGGCCC TCGGCGCGTT CGTACTGTTC
            541 CACGATGGTG TAGTCCTCGT TGTGGGAGGT GATGTCCAAC TTGATGTTGA CGTTGTAGGC
            601 GCCGGGCAGC TGCACGGGCT TCTTGGCCTT GTAGGTGGTC TTGACCTCAG CGTCGTAGTG
            661 GCCGCCGTCC TTCAGCTTCA GCCTCTGCTT GATCTCGCCC TTCAGGGCGC CGTCCTCGGG
            721 GTACATCCGC TCGGAGGAGG CCTCCCAGCC CATGGTCTTC TTCTGCATTA CGGGGCCGTC
            781 GGAGGGGAAG TTGGTGCCGC GCAGCTTCAC CTTGTAGATG AACTCGCCGT CCTGCAGGGA
            841 GGAGTCCTGG GTCACGGTCA CCACGCCGCC GTCCTCGAAG TTCATCACGC GCTCCCACTT
            901 GAAGCCCTCG GGGAAGGACA GCTTCAAGTA GTCGGGGATG TCGGCGGGGT GCTTCACGTA
            961 GGCCTTGGAG CCGTACATGA ACTGAGGGAC AGGATGTCCC AGGCGAAAGG GCAGGGGCCA
           1021 CCCTTGGTCA CCTTCAGCTT GGCGGTCTGG GGTGCCCCTC GTAGGGGCGC CCTCGCCCTC
           1081 GCCCTCGATC TCGAACTCGT ATCGTTCACG GAGCCCTCCA TGGTGCAACT GGAGCGCAAT
          1141 GGACTCATGG ATGATGGGCC ATGTTTATCC TTCCCTCGGC CCCTGTGCTT CT
          //
          Primer: CMV-Forward
          Sequence file: RDB15269_A7H3c.seq check
          >D04498H9-1_A7H3_CMV-Forward_C03_09_ABI24.ab1
              1 TTGTCAACTC CGCCCCATTG ACGCAATGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC
            121 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC
            181 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT
            241 AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA
            301 ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA
            361 GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA
            421 TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG
            481 GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA
            541 GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT
            601 ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA
            661 TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC
            721 TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGGGCCGCT
            781 GATCTTCAGA CCTGGAGGAG GAGATATGAG GGACAATTGG AGAAGTGAAT TATATAAATA
            841 TAAAGTAGTA AAAATTGAAC CATTAGGAGT AGCACCCACC AAGGCAAAGA GAAGAGTGGT
            901 GCAGAGAGAA AAAAGAGCAG TGGGAATAGG AGCTTTGTTC CTTGGGTTCT TGGAGCAGCA
            961 GGAAGCACTA TGGGCGCAGC GTCAATGACG CTGACGGTAC AGGCCAGACA ATTTATTGTC
           1021 TGGTATAGTG CAGCAGCAGA ACAAATTTGC TGAGGGCTAT TGAGCGCAAC AGCAATCTGG
           1081 TGGCAACTCA ACAGGTCTGG GGCATCAAGC AGCTTCAAGG CAGATTCCTG ACTGATGAAA
          1141 GAAATACCTT AAAAGGAATT C
          //
          Primer: BGH_rev2
          Sequence file: RDB15269_A7H3d.seq check
          >D04498H9-1_A7H3_BGH_rev2_C04_07_ABI24.ab1
              1 CAGGTCGGGT CAGTAGCACG GGGGAGGGGC AACAAGCAGA TGGCTGGCAA CTAGAAGGCA
             61 CAGTGCGAGG CTGATCAGCG GGTTTAAACG GGCCCTGCTA GAGATTTTCC ACACTGACTA
            121 AAAGGGTCTG AGGGATCTCT AGTTACCAGA GTCACACAAC AGACGGGCAC ACACTACTTG
            181 AAGCACTCAA GGCAAGCTTT ATTGAGGCTT AAGCAGTGGG TTCCCTAGTT AGCCAGAGAG
            241 CTCCCAGGCT CAGATCTGGT CTAACCAGAG AGACCCAGTA CAGTCCGGAT GCAGCTCTCG
            301 GGCCATGTGA TGAAATGCTA GGCGGCTGTC AAACCTCCAC TCTAATACTT CTCTCTCCGG
            361 GTCATCCATC CCATGCAGGC TCACAGGGTG TAACAAGCGG GTGTTCTCTC CTTCATTGGC
            421 TTCTTCTACC TTCTCTTGCT CAACTGGTAC TAGCTTGTAG CACCATCCAA AGGTCAGTGG
            481 ATATCTGATC CCTGGCCCTG GTGTGTAGTT CTGCCAATCA GGGAAGTAGC CTTGTGTGTG
            541 GTAGATCCAC AGATCAAGGA TATCTTGTCT TCGTTGGGAG TGAATTAGCC CTTCCAGTCC
            601 CCCCTTTTCT TTTAAAAAGT GGCTAAGATC TACAGCTGCC TTGTAAGTCA TTGGTCTTAA
            661 AGGTACCTGA GGTGTGACTG GAAAACCCAC CTCCTCCTCC TCTTGTGCTT CTAGCCAGGC
            721 ACAATCAGCA TTGGTAGCTG CTGTATTGCT ACTTGTGATT GCTCCATGTT TTTCTAGGTC
            781 TCGACGGTAT CGATGCGGGG AGGCGGCCCA AAGGGAGATC CGACTCGTCT GAGGGCGAAG
            841 GCGAAGACGC GGAAGAGGCC GCAGAGCCGG CAGCAGGCCG CGGGAAGGAA GGTCCGCTGG
            901 ATTGAGGGCC GAAGGGACGT AGCAGAAGGA CGTCCCGCGC AGAATCCAGG TGGCAACACA
            961 GGCGAGCAGC CATGGAAAGG ACGTCAGCTT TCCCCGACAA CACCACGGGA ATTTGTCAGT
           1021 GCCCAACAGC CCGAGCCCCT GTCCAGCAGC GGGCAAGGCA GGCGGCGATG AGTTCCGCCG
           1081 TGCATTAGGA GGGGGAAGCG AAGTCCGGAA AGAGCTGACA GTGTGGCATG CCTCACCAGC
          1141 TGGGGGGTTG GGCGGTCCAG GCC
          //
          Primer: SV40pro_F
          Sequence file: RDB15269_A7H3e.seq check
          >D04498H9-1_A7H3_SV40pro_F_C05_08_ABI24.ab1
              1 CCTGGCCCCG TGGCATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
             61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA
            121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC
            181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC
            241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC
            301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC
            361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG
            421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG
            481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG
            541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC
            601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG
            661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT
            721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC
            781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG
            841 TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC
            901 ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA
            961 TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT
           1021 TAATGAATCG GCCCAACGCG CGGGGGAGAG GCGGTTTGCG TATTTGGGGC GGCTTCTTCG
           1081 CTTCTCGCTT CACTTGACTC GCTGCGCTCG ATCGGTTTCG GGCTGCGCCA GCCGGTAATC
          1141 AGCTTCACAT GAAGACGGTT AGATACCTGC TGAATCCCTC ACCAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sakaue-Sawano, A., Tracing the silhouette of individual cells in S/G2/M phases with fluorescence. Chem. Biol. 15 (12): 1243-1248 (2008). PMID 19101468.
          reference Zielke, N., FUCCI sensors: powerful new tools for analysis of cell proliferation. Wiley Interdiscip. Rev. Dev. Biol. 4 (5): 469-487 (2015). PMID 25827130.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl