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mKO2-hCdt1(30/120) / pCSII-EF

Lentivirus vector plasmid of Fucci-G1 phase probe. Component of Fucci cell cycle probe.

Catalog number RDB15267
Resource name mKO2-hCdt1(30/120) / pCSII-EF
Alternative name FUCCI-Red
Clone info. Lentivirus vector plasmid of Fucci-G1 phase probe. One of the component of Fucci cell cycle probe. Fluorescence was detected in the nucleus.
Comment Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Vector backbone CSII-EF-MCS (Plasmid)
Size of vector backbone 9.1 kb
Selectable markers Please note that Zeo resistance marker is not included in the lentivirus produced from this vector. E. coli: Amp^r.
Gene/insert name Human CDT1 cDNA Fungia concinna OFP cDNA
Depositor|Developer Miyawaki, Atsushi |
Other clones in our bank

External Database
Human CDT1 Fungia concinna OFP

          Reference sequence
            
           
          Lentiviral plasmid list. [link]

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano, A. et al. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell, 132 (3): 487-498.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          Additional terms and conditions:
          The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
          Ordering Please visit Ordering instruction.[link] 
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          Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 1. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial.
          2. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested.
          Sakaue-Sawano, A. et al. (2008) Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell, 132 (3): 487-498.
          3. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL.
          4. The Recipient's Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
          付加的提供条件:
          レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
          提供依頼 手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (非営利機関による非営利学術研究用)[Word]
          遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
          備考 【追加提供依頼書】受入れ確認書(書式J)
          このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
          提供条件は英文のまま記載してください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB15267 mKO2-hCdt1(30/120) / pCSII-EF DNA solution

          Ordering instruction of plasmids [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The mKO2-hCdt1(30/120) / pCSII-EF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15267).

          Reference section:

          Sakaue-Sawano, A., Kurokawa, H., Morimura, T., Hanyu, A., Hama, H., Osawa, H., Kashiwagi, S., Fukami, K., Miyata, T., Miyoshi, H., Imamura, T., Ogawa, M., Masai, H., Miyawaki, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Fucci-related materials (en) (English text)
          Featured content Fucci-related materials (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB15267_A7H3p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F (Pr0168)
          Region: EF-1 alpha pro,insert 5'(mKO2 5')
          Sequence file: RDB15267_A7H3g.seq check
          >D04498H7-1_A7H3_EF1a-C_F_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNTNNNNNNN NNNNNNNTTT GNNNCTTGGT TNNTTCTCNN GCCTNNAGAC
             61 AGTGGTTCAA AGTTTTTTTC TTCCATTTCA GGTGTCGTGA ACACGCTACC GGTCTCGAGA
            121 ATTCGCCACC ATGGTGAGTG TGATTAAACC AGAGATGAAG ATGAGGTACT ACATGGACGG
            181 CTCCGTCAAT GGGCATGAGT TCACAATTGA AGGTGAAGGC ACAGGCAGAC CTTACGAGGG
            241 ACATCAAGAG ATGACACTAC GCGTCACAAT GGCCGAGGGC GGGCCAATGC CTTTCGCGTT
            301 TGACTTAGTG TCACACGTGT TCTGTTACGG CCACAGAGTA TTTACTAAAT ATCCAGAAGA
            361 GATACCAGAC TATTTCAAAC AAGCATTTCC TGAAGGCCTG TCATGGGAAA GGTCGTTGGA
            421 GTTCGAAGAT GGTGGGTCCG CTTCAGTCAG TGCGCATATA AGCCTTAGAG GAAACACCTT
            481 CTACCACAAA TCCAAATTTA CTGGGGTTAA CTTTCCTGCC GATGGTCCTA TCATGCAAAA
            541 CCAAAGTGTT GATTGGGAGC CATCAACCGA GAAAATTACT GCCAGCGACG GAGTTCTGAA
            601 GGGTGATGTT ACGATGTACC TAAAACTTGA AGGAGGCGGC AATCACAAAT GCCAAATGAA
            661 GACTACTTAC AAGGCGGCAA AAGANATTCT TGAAATGNCA GGAGACCATT ACATCGGCCA
            721 TCGCCTCGTC AGGAAAACCG AANGNAACAT TACTGAGCAG GTAGAAGANG CAGTAGCTCA
            781 TTACTCNATG TTGCCTTCCC AGGGCGGANA TCCNTCACAC TGGCGGCCGC TCGAGCCCAG
            841 CCCCGNNAGG CCCGCACTCC GCGCCCCNGC CTNCGCTNNC AGTGGCANNC GCAAGCGCGC
            901 CCGCCCGCCC GCCGCCCCCG GANNCNANNA NGNCNNGGNC ANNGGCCCNN NNNANNNTGC
            961 NNNTGNCGGN GGNNGAGGTT TCCNNCCCCN NNANCCCCNA NGNCCCNNAN NTNCNNNNCN
          1021 NGNCNNNNNN NNGNNNANAA NNNNANNANN NNNNCCCNNN NNNNNNN
          //
          Primer: PRE_rev (Pr0250)
          Region: PRE,insert 3'(hCdt1(30/120),mKO2 3')
          Sequence file: RDB15267_A7H3h.seq check
          >D04498H7-1_A7H3_PRE_rev_C09_09_ABI24.ab1
              1 NNNNNNNNNN NNCGNNNNGG AGCNNCNTAG TTAAGAATAC CAGTCAATCT TTCACAAATT
             61 TTGTAATCCA GAGGTTGATT ATCGATAAGC TTGATCCCTC GATGTTAACT CTAGATTAGA
            121 TGGTGTCCTG GTCCTGCGCG GATGTCAGGT GGGGCGGCTG ACCTGCTGCC GGGGTGGATT
            181 TCTTTATCTT CTGGCCCGGA GAAGGGCAGG CTGGGATGTC TGGGGCCTCG GGGGTACTGG
            241 GGCTGGAAAC CTCGTCCACC GACAGCCGCA GTCTCCTGCG GGCCGGTGGC CTGGCCTGGT
            301 CGCGTCCGGG GGCGGCGGGC GGGCGGGCGC GCTTGCGGCT GCCACTGGTA GCGGAGGCCG
            361 GGGCGCGGAG TGCGGGCCTG GCGGGGCTGG GCTCGAGCGG CCGCCAGTGT GATGGATATC
            421 CGCCCTGGGA AGGCAACATT GAGTAATGAG CTACTGCATC TTCTACCTGC TCAGTAATGT
            481 TGCCTTCGGT TTTCCTGACG AGGCGATGGC CGATGTAATG GTCTCCTGGC ATTTCAAGAA
            541 TCTCTTTTGC CGCCTTGTAA GTAGTCTTCA TTTGGCATTT GTGATTGCCG CCTCCTTCAA
            601 GTTTTAGGTA CATCGTAACA TCACCCTTCA GAACTCCGTC GCTGGCAGTA ATTTTCTCGG
            661 TTGATGGCTC CCAATCAACA CTTTGGTTTT GCATGATAGG ACCATCGGCA GGAAAGTTAA
            721 CCCCAGTAAA TTTGGATTTG TGGTAGAANG TGTTTCCTCT AANGCTTATA TGCGCACTGA
            781 CTGAAGCGGA NCCACCATCT TCNAACTCCA ACGACCTTTC CNNTGACANG NCTTCANGAA
            841 ATGCTNGTTT GAAATANTCT GGTATCTCTT CTGGATANTN NGTAAATACT CTGTGGNCGT
            901 AACAGAACAN GTGNGNNNCT AANNCAAACN CGAANNNNNT TGGNCCNNCN NNNGNCNATT
          961 GNNNNNCNNA NNGNCNTNNN NNGANNNNCN NNNNNANNNN NNNNNN
          //
          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,5' LTR,HIV-1 psi
          Sequence file: RDB15267_A7H3i.seq check
          >D04498H7-1_A7H3_CMV-Forward_D09_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNTTGN NGCNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
            121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
            181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
            241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
            301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
            361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
            421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
            481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
            541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
            601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
            661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
            721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGANCA CCGCACAGCA NGGGCCGCTG
            781 ATCTTCAGAC CTGGAGGANG ANNNATGANG GANNATTGGA GAAGTGAATT ATATAAATAT
            841 AAAGTAGTAA AAATTGNANC NTTAGGAGTA GCACCCACNN AGGCNAAGAN AAGANTGNTG
            901 CAGANAGAAA AAANAGCAGN GGGAATAGGA NCTTTGTTCC TNNGNNTCNT GGGANCANCN
          961 NNNNNCACTN NNGGNNNANC GNNNNNNNNG NNNNNNNNNN
          //
          Primer: cPPT/CTS-E_R (Pr0725)
          Region: cPPT_CTS,RRE
          Sequence file: RDB15267_A7H3j.seq check
          >15267_15267_B0B4F_cPPT_CTS-E_R_E02_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNTTCTTNA GTTTGTATGT CTGTTGCTAT TATGTCTACT
             61 ATTCTTTCCC CTGCACTGTA CCCCCCAATC CCCCCTTTTC TTTTAAAATT GTGGATGAAT
            121 ACTGCCATTT GTAGATCCGT TCACTAATCG AATGGATCTG TCTCTGTCTC TCTCTCCACC
            181 TTCTTCTTCT ATTCCTTCGG GCCTGTCGGG TCCCCTCGGG GTTGGGAGGT GGGTCTGAAA
            241 CGATAATGGT GAATATCCCT GCCTAACTCT ATTCACTATA GAAAGTACAG CAAAAACTAT
            301 TCTTAAACCT ACCAAGCCTC CTACTATCAT TATGAATAAT TTTATATACC ACAGCCAATT
            361 TGTTATGTTA AACCAATTCC ACAAACTTGC CCATTTATCT AATTCCAATA ATTCTTGTTC
            421 ATTCTTTTCT TGCTGGTTTT GCGATTCTTC AATTAAGGAG TGTATTAAGC TTGTGTAATT
            481 GTTAATTTCT CTGTCCCACT CCATCCAGGT CGTGTGATTC CAAATCTGTT CCAGAGATTT
            541 ATTACTCCAA CTAGCATTCC AAGGCACAGC AGTGGTGCAA ATGAGTTTTC CAGAGCAACC
            601 CCAAATCCCC AGGAGCTGTT GATCCTTTAG GTATCTTTCC ACAGCCAGGA TTCTTGCCTG
            661 GAGCTGCTTG ATGCCCCAGA CTGTGAGTTG CAACAGATGC TGTTGCGCCT CAATAGCCCT
            721 CAGCAAATTG TTCTGCTGCT GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG
            781 CGTCATTGAC GCTGCGCCCA TANTGCTTCC TGCTGCTCCN NAGAACCCAA GGAACAAAGC
            841 TCCTATTCCC ACTGCTCTTT TTTTCTCTCT GCACCACTCT TCTCTTTNNC NNNGNGGGNG
            901 CTACTCCTAA NGGNTCAATT TTNACNANNT TATANTTANA TNNNTNNACN NNTNCNNNNT
          961 NNNCCNNNAT ATNNNCNNNC NNNCNNNGNN NNNNNNANNN NNNNNNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA,3' LTR
          Sequence file: RDB15267_A7H3k.seq check
          >D04498H7-1_A7H3_BGH_rev2_A04_01_ABI24.ab1
              1 NNNNNNNNNN NNGNNGNNNG GGGGNNGGNN NNNNACAGAT GGCTGGCAAC TAGAAGGCAC
             61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA
            121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA
            181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT
            241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG
            301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT
            361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT
            421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT
            481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT
            541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC
            601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG
            661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC
            721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTNTN
            781 NNCGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCNTCTGA NGGCGAANGN
            841 GAAGACGCGG AAGNNNNNNC ANAGNCNGCN NNNNGCCGCG GGAANGAAGG TCCGCTNGNA
            901 TTGAGGGCCG AAGGGNCNTN NCANAANNAN GTCCCNNNNN NANNNNNNGT GGCNNCNCNN
          961 NNNANNANNN ATGGAAANGN NNTCAGCNTN CCCNNACNNC NCCNCNNNNN NNNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: SV40 pro_ori,Zeo,SV40 pA
          Sequence file: RDB15267_A7H3l.seq check
          >D04498H7-1_A7H3_SV40pro_F_A05_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT GANGANGNTT TTTTGGAGGC CTAGGCTTTT
             61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA
            121 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT
            181 GGCCAAGTTG ACCAGTGCCG TTCCGGTGCT CACCGCGCGC GACGTCGCCG GAGCGGTCGA
            241 GTTCTGGACC GACCGGCTCG GGTTCTCCCG GGACTTCGTG GAGGACGACT TCGCCGGTGT
            301 GGTCCGGGAC GACGTGACCC TGTTCATCAG CGCGGTCCAG GACCAGGTGG TGCCGGACAA
            361 CACCCTGGCC TGGGTGTGGG TGCGCGGCCT GGACGAGCTG TACGCCGAGT GGTCGGAGGT
            421 CGTGTCCACG AACTTCCGGG ACGCCTCCGG GCCGGCCATG ACCGAGATCG GCGAGCAGCC
            481 GTGGGGGCGG GAGTTCGCCC TGCGCGACCC GGCCGGCAAC TGCGTGCACT TCGTGGCCGA
            541 GGAGCAGGAC TGACACGTGC TACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT
            601 GGGCTTCGGA ATCGTTTTCC GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT
            661 GCTGGAGTTC TTCGCCCACC CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG
            721 CAATAGCATC ACAAATTTCA CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT
            781 GTCCNAACTC ATCAATGTAT CTTATCATGT CTGTATACCG TCNACCTCTA GCTAGAGCTN
            841 GGCGTAATCA TGGTCATAGC TGTTTCCTGT GTGAAATTGN NATCCGCTCN CAATNNCACA
            901 CAACATACGA GCCNGAAGCA TNANGTGNAA AGCCNGGGGN GNNNAANGAG TGANCTAANT
            961 CNCNTTAATT GCGTTGNNCT CACNNNCNNN NTTNNNGNNN GNNANNNNGN CNNGNNNNCN
          1021 GCATNNNNNN ANNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sakaue-Sawano, A., Visualizing spatiotemporal dynamics of multicellular cell-cycle progression. Cell 132 (3): 487-498 (2008). PMID 18267078.
          reference Möller, J., Process-induced cell cycle oscillations in CHO cultures: Online monitoring and model-based investigation. Biotechnol. Bioeng. 116 (11): 2931-2943 (2019). PMID 31342512.
          reference Takahashi, H., Significance of Polypyrimidine Tract-Binding Protein 1 Expression in Colorectal Cancer. Mol. Cancer. Ther. 14 (7): 1705-1716 (2015). PMID 25904505.
          reference Beaumont, K.A., Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids. J. Vis. Exp. (106):e53486 (2015). PMID 26779761.
          reference Kagawa, Y., Cell Cycle-Dependent Rho GTPase Activity Dynamically Regulates Cancer Cell Motility and Invasion In Vivo. PLoS One, 8 (12): e83629 (2013). PMID 24386239.
          user_report Tanaka, A., Reversible expansion of pancreatic islet progenitors derived from human induced pluripotent stem cells. Genes Cells 25 (5): 302-311 (2020). PMID 32065490.
          user_report Onozato, Y., Radiosensitivity of quiescent and proliferating cells grown as multicellular tumor spheroids. Cancer Sci. 108 (4): 704-712 (2017). PMID 28135769.
          user_report Sereti, K.I., Methods to study the proliferation and differentiation of cardiac side population (CSP) cells. Methods Mol. Biol. 1036: 95-106 (2013). PMID 23807790.

          2023.05.22

          GNP_filter3_RDBDEP_html_230512.pl