Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF-FLAG-mouse SNAP-25

Expression vector of mouse synaptosomal-associated protein 25 (SNAP-25).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (J. Biol. Chem. 277 (33), 30351-30358, 2002) Additional terms and conditions: Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.

Ordering

Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Biol. Chem. 277 (33), 30351-30358, 2002)。 付加的提供条件： pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15176_A7Fmp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Region: EF-1 alpha pro, FLAG, insert, hGCSF+p(A) Sequence file: RDB15176_A7Fma.seq
>D04709B2_1_A7Fm_EF1a-C_F_B03_06_ABI24.ab1     1 TCAGCATGCC TTTTTGAGTT TGGATCTTGG TTCATTCTCA AGCCTCAGAC AGTGGTTCAA    61 AGTTTTTTTC TTCCATTTCA GGTGTCGTGA GGAATTCTCT AGAGCGGCCG CGGGAATTCG   121 ATTCCACCAT GGACTACAAG GACGATGACG ACAAGGGATC CATGGCCGAG GACGCAGACA   181 TGCGCAATGA GCTGGAGGAG ATGCAGAGGA GGGCTGACCA GCTGGCTGAT GAGTCCCTGG   241 AAAGCACCCG TCGCATGCTG CAGCTGGTTG AAGAGAGTAA AGATGCTGGC ATCAGGACTT   301 TGGTTATGTT GGATGAGCAA GGCGAACAAC TGGAACGCAT TGAGGAAGGG ATGGACCAAA   361 TCAATAAGGA CATGAAAGAA GCAGAAAAGA ATTTGACGGA CCTAGGAAAA TTCTGCGGGC   421 TTTGTGTGTG TCCCTGTAAC AAGCTTAAAT CCAGTGATGC TTACAAAAAA GCCTGGGGCA   481 ATAATCAGGA TGGAGTAGTG GCCAGCCAGC CTGCCCGTGT GGTGGATGAA CGGGAGCAGA   541 TGGCCATCAG TGGTGGCTTC ATCCGCAGGG TAACAAATGA TGCCCGGGAA AATGAGATGG   601 ATGAGAACCT GGAGCAGGTG AGCGGCATCA TCGGAAACCT CCGCCATATG GCTCTAGACA   661 TGGGCAATGA GATTGACACC CAGAATCGCC AGATCGACAG GATCATGGAG AAGGCTGATT   721 CCAACAAAAC CAGAATTGAT GAAGCCAACC AACGTGCAAC AAAGATGCTG GGAAGTGGTT   781 AATCACTAGT GAATTCGCGG CCGCTCTAGA GTGAGGGTCC CCACCTGGGA CCCTTGAGAG   841 TATCAGTCTC CCACGTGGGA GACAAGAAAT CCCTGTTTAA TATTTAAACA GCAGTGTTCC   901 CCATCTGGGT CCTTGCACCC CTCACTCTGG CCTCAGCCGA CTGCACAGCG GCCCCTGCAT   961 CCCCTTGGCT GTGAGGCCCC TGGACAAGCA GAGGTGGCCA GAGCTGGGAG GCATGGCCCT  1021 GGGGTCCCAC GATTTGCTGG GGATCTCGTT TTTCTTCTAG ACTTTTGGGA CATGATTTGA  1081 CTCCCGAACA TCAACGAACG TGTCTCTGCT TTTTCTGGAT GCTCGAACGC TGGTCTGGCC 1141 CCACGAGGTC AGACTGTGAC TCTTTTTTTA AGGGG //
Primer: hGCSF-R Region: hGCSF+p(A), insert, FLAG, EF-1 alpha pro Sequence file: RDB15176_A7Fmb.seq
>D04709B2_1_A7Fm_hGCSF-R_C03_09_ABI24.ab1     1 TGGGGAAATG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGCG GCCGCGAATT    61 CACTAGTGAT TAACCACTTC CCAGCATCTT TGTTGCACGT TGGTTGGCTT CATCAATTCT   121 GGTTTTGTTG GAATCAGCCT TCTCCATGAT CCTGTCGATC TGGCGATTCT GGGTGTCAAT   181 CTCATTGCCC ATGTCTAGAG CCATATGGCG GAGGTTTCCG ATGATGCCGC TCACCTGCTC   241 CAGGTTCTCA TCCATCTCAT TTTCCCGGGC ATCATTTGTT ACCCTGCGGA TGAAGCCACC   301 ACTGATGGCC ATCTGCTCCC GTTCATCCAC CACACGGGCA GGCTGGCTGG CCACTACTCC   361 ATCCTGATTA TTGCCCCAGG CTTTTTTGTA AGCATCACTG GATTTAAGCT TGTTACAGGG   421 ACACACACAA AGCCCGCAGA ATTTTCCTAG GTCCGTCAAA TTCTTTTCTG CTTCTTTCAT   481 GTCCTTATTG ATTTGGTCCA TCCCTTCCTC AATGCGTTCC AGTTGTTCGC CTTGCTCATC   541 CAACATAACC AAAGTCCTGA TGCCAGCATC TTTACTCTCT TCAACCAGCT GCAGCATGCG   601 ACGGGTGCTT TCCAGGGACT CATCAGCCAG CTGGTCAGCC CTCCTCTGCA TCTCCTCCAG   661 CTCATTGCGC ATGTCTGCGT CCTCGGCCAT GGATCCCTTG TCGTCATCGT CCTTGTAGTC   721 CATGGTGGAA TCGAATTCCC GCGGCCGCTC TAGAGAATTC CTCACGACAC CTGAAATGGA   781 AGAAAAAAAC TTTGAACCAC TGTCTGAGGC TTGAGAATGA ACCAAGATCC AAACTCAAAA   841 AGGGCAAATT CCAAGGAGAA TTACATCAAG TGCCAAGCTG GCCTAACTTC AGTCTCCACC   901 CACTCAGTGT GGGGAAACTC CATCGCATAA AACCCCTCCC CCCAACCTAA AGACGACGTA   961 CTCCAAAAGC TCGAGAACTA ATCGAGGTGC CTGGACGGCG CCCGGTACTC CGTGGAGTCA  1021 CATGAAGCGA CGGCTGAGGA CGGGAAAGGC CCTTTTCCTT TTGTGTGGGT GACTCACCGG  1081 CCCGCTCTCC CGAGCGCCGC GTCCTCCATT TTGAGCCTCC CTGCAGCCAG GGACGGAGCG 1141 GTCAATCTTT CCGCTCACGC AACTGGGTGG CCCGGACCCG GGGGCCAGAG CCTC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.10.11

GNP_filter3_RDBDEP_html_221010.pl