RDB15145 - Ratiometric pericam-nu/pcDNA3

Calcium ion sensor clone Ratiometric pericam-nu tagged with nuclear localization signal.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki
Remarks	Please obtain written approval of Dr. Atsushi Miyawaki.

提供案内 (日本国内)

[open/close]

提供条件	1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Nagai, T., Sawano, A., Park, E.S., Miyawaki, A. (2001) Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the MATERIAL. 5. The Recipient’s Scientist agrees not to transfer the MATERIAL to any third party including any resource centers without the prior written consent of DEPOSITOR.
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word]
備考	提供条件は英文のまま記載してください。宮脇敦史博士の書面による提供承諾が必要です。

References and tips

QC test results

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB15145	Ratiometric pericam-nu/pcDNA3	DNA solution

original	Nagai, T., Circularly permuted green fluorescent proteins engineered to sense Ca2+. Proc. Natl. Acad. Sci. U.S.A. 98 (6): 3197-3202 (2001). PMID 11248055.

Featured content	Calcium Ion Sensor Clones (English text)

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: CMV_Forward (Pr0016) Region: CMV pro, insert 5' Sequence file: RDB15145_A7H9a.seq
>D04498D3_A7H9_1-CMV-Forward_E08_14_ABI24.ab1 1 ACGGATTAAA TTCGGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT 61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA 121 ATACGACTCA CTATAGGGAG ACCCAAGCTT GCGGCCGCCA CCATGGTGAA GAGGCGCTGG 181 AAGAAAAACT TCATTGCCGT CAGCGCTGCC AACCGGTTCA AGAAGATCTC CAGCTCCGGG 241 GCACTGGGGT CTGCAGGCTA CAACAGCGAC AACGTCTATA TCATGGCCGA CAAGCAGAAG 301 AACGGCATCA AGGCCAACTT CAAGATCCGC CACAACATCG AGGACGGCGG CGTGCAGCTC 361 GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC 421 CACTACCTGA GCTTCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG 481 GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG 541 GTCGACGGTG GCAGCGGTGG CACCGGTGTG AGCAAGGGCG AGGAGCTGTT CACCGGGGTG 601 GTGCCCATCC TGGTCGAGCT GGACGGCGAC GTAAACGGCC ACAAGTTCAG CGTGTCCGGC 661 GAGGGCGAGG GCGATGCCAC CTACGGCAAG CTGACCCTGA AGCTCATCTG CACCACCGGC 721 AAGCTGCCCG TGCCCTGGCC CACCCTCGTG ACCACCTTCG GCTACGGCCT GAAGTGCTTC 781 GCCCGCTACC CCGACCACAT GAAGCAGCAC GACTTCTTCA AGTCCGCCAT GCCCGAAAGC 841 TACGTCCAGG AGCGCACCAT CTTCTTCAAG GACGACGGCA ACTACAAGAC CCGCGCCGAG 901 GTGAAGTTCG AGGGCGACAC CCTGGTGAAC CGCATCGAGC TGAAGGGCAT CGACTTCAAG 961 GAGGACGGCA ACATCCTGGG GCACAAGCTG GGAGTACAAC GGTACCGAAC CAACTGACAG 1021 AAGAGCAGAT TGCAGAGTTC AAAGAAGCCC TTTCTCATTA TTTCGACAAG GAATGGGGAC 1081 GGCACCATCA CCACAAAAGA CCTGCACGTT ATGAGTTCGC TTGGACAAAA ACCCAAACGG 1141 AAAGCACAGA //
Primer: BGH_rev2 (Pr0606) Region: bGH pA, insert 3' Sequence file: RDB15145_A7H9b.seq
>D04498D3_A7H9_1-BGH_rev2_F08_17_ABI24.ab1 1 CATTACGGGG GTCAGAAGGC ACGGGGGGAG GGGCAAACAA CAGATGGCTG GCAACTAGAA 61 GGCACAGTCG AGGCTGATCA GCGAGCTCTA GCATTTAGGT GACACTATAG AATAGGGCCC 121 TCTAGATGCA TGCTCGAGCG GCCGCCAGTG TGATGGATAT CTGCAGAATT CTTAGGCGTC 181 CTCCACCTTC CTCTTCTTCT TGGGCTTTGC TGTCATCATT TGTACAAACT CTTCATAGTT 241 TACTTGGCCA TCACCATCGA TATCTGCTTC CCTTATCATT TCATCAACTT CTTCATCTGT 301 TAACTTCTCC CCGAGGTTTG TCATGACGTG ACGTAATTCA GCAGCGCTGA TGTAGCCGTT 361 CCCATCCTTG TCAAAAACAC GGAATGCTTC TCGGATTTCC TCTTCGCTGT CTGTGTCCTT 421 CATTTTTCTA GCCATCATAG TAAGAAATTC AGGAAAGTAA ATCGTTCCAT TGCCATCAGC 481 ATCGACTTCA TTGATCATAT CCTGCAATTC TGCTTCCGTT GGGTTTTGTC CAAGCGACCT 541 CATAACGGTG CCAAGTTCCT TTGTGGTGAT GGTGCCGTCC CCATCCTTGT CGAATAATGA 601 GAAGGCTTCT TTGAACTCTG CAATCTGCTC TTCTGTCAGT TGGTCGGTAC CGTTGTACTC 661 CAGCTTGTGC CCCAGGATGT TGCCGTCCTC CTTGAAGTCG ATGCCCTTCA GCTCGATGCG 721 GTTCACCAGG GTGTCGCCCT CGAACTTCAC CTCGGCGCGG GTCTTGTAGT TGCCGTCGTC 781 CTTGAAGAAG ATGGTGCGCT CCTGGACGTA GCCTTCGGGG CATGGCGGAC TTGAAGAAGT 841 CGTGCTGCTT CATGTGGTCG GGGTAGCGGG CGAAGCACTT CAGGCCGTAG CCGAAGGTGG 901 TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT GCAGATGAGC TTCAGGGTCA 961 GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC GCTGAAACTT GTGGCCGGTT 1021 TACGTCGCCC GTCCAGCTCG GACCAAGGAT GGGGCACACC CCGGGTGAAA CAGCTTCCTC 1081 GCCCTGGCTC ACACGGTGGC CAACCGCTGG CCACGTTCGA ACTGTACAAG CTCGGTTCCA 1141 TGTCCTGAGG AGATGGAAAT TCCCTCCGGC //
Primer: SV40pro_F (Pr0110) Region: SV40 pro_ori, NeoR Sequence file: RDB15145_A7H9c.seq
>D04498D3_A7H9_1-SV40pro_F_G08_20_ABI24.ab1 1 ACTTGGTCCT CGGAACATTC AGAGGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG 61 CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG 121 ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA 181 GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT 241 CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT 301 GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG 361 CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT 421 GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC 481 TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC 541 GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA 601 TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG 661 CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT 721 GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG 781 CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC 841 TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA 901 TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGG TTCGAAATGA CCGACCAAGC 961 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG 1021 CTTTCGGGAA TCGTTTTCCC GGGACGCCGG CTGGGATGAT CCTCCCAGCG CGGGGATCTC 1081 AATGCTGGAA GTTCTTCGCC ACCCTACCTG GTTATTTGCA GCTTATAATT GGTTACCAAC 1141 TAAAGGC //