Cloning vector to add a triple repeat of the amino acid linker Gly-Gly-Gly-Gly-Ser [(GGGGS)x3].
|
Other clones in our bank
External Database Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
---|---|
Ordering | Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form [Word] by Dr. Atsushi Miyawaki Please visit Information of Request for Distribution.[link] |
Remarks | Please obtain written approval of Dr. Atsushi Miyawaki. |
提供条件 | 1. The RECIPIENT should obtain written approval from the DEPOSITOR using the APPROVAL FORM prior to entering the AGREEMENT with the RIKEN BRC. 2. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. 3. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR or an acknowledgment to the DEPOSITOR are requested. Shimozono S, Miyawaki A. (2008) Engineering FRET Constructs Using CFP and YFP Methods Cell Biol. 85C: 381-393. 4. The RECIPIENT agrees to provide a copy of the publication to the DEPOSITOR, oral or written, reporting use of the BIOLOGICAL RESOURCE. 5. The Recipient's Scientist agrees not to transfer the BIOLOGICAL RESOURCE to any third party including any resource centers without the prior written consent of DEPOSITOR. |
---|---|
提供依頼 | 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 宮脇敦史博士による提供承諾書 [Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
備考 | 提供条件は英文のまま記載してください。 宮脇敦史博士の書面による提供承諾が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
---|---|---|---|
RDB15127 | pBS coupler 2 | DNA solution |
Materials & Methods section:
The pBS coupler 2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB15127). |
Reference section:
Shimozono, S., Miyawaki, A., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Empty Backbone (English text) |
---|---|
Featured content | Empty Backbone (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB15127_A7H9p1-2.pdf |
---|
Nucleotide sequence of a portion of this resource (if available).
Primer: M13R (Pr0160) Region: insert, f1 ori Sequence file: RDB15127_A7H9a.seq |
---|
>D04498B3_A7H9_1_M13R_G03_21_ABI24.ab1 1 ACCGCCCCCA ATTTAACCCT TCACTAAAGG GGAACAAAAG CTGGGGTACC GGGCCCCCCC 61 TCGAGGTCGA CGGTATCGAT AAGCTTGATG GTGGAGGCGG TTCAGGCGGA GGTGGCTCTG 121 GCGGTGGCGG ATCGCATCGA ATTCCTGCAG CCCGGGGGAT CCACTAGTTC TAGAGCGGCC 181 GCCACCGCGG TGGAGCTCCA ATTCGCCCTA TAGTGAGTCG TATTACGCGC GCTCACTGGC 241 CGTCGTTTTA CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC 301 AGCACATCCC CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC 361 CCAACAGTTG CGCAGCCTGA ATGGCGAATG GAAATTGTAA GCGTTAATAT TTTGTTAAAA 421 TTCGCGTTAA ATTTTTGTTA AATCAGCTCA TTTTTTAACC AATAGGCCGA AATCGGCAAA 481 ATCCCTTATA AATCAAAAGA ATAGACCGAG ATAGGGTTGA GTGTTGTTCC AGTTTGGAAC 541 AAGAGTCCAC TATTAAAGAA CGTGGACTCC AACGTCAAAG GGCGAAAAAC CGTCTATCAG 601 GGCGATGGCC CACTACGTGA ACCATCACCC TAATCAAGTT TTTTGGGGTC GAGGTGCCGT 661 AAAGCACTAA ATCGGAACCC TAAAGGGAGC CCCCGATTTA GAGCTTGACG GGGAAAGCCG 721 GCGAACGTGG CGAGAAAGGA AGGGAAGAAA GCGAAAGGAG CGGGGCGCTA GGGCGCTGGC 781 AAGTGTAGCG GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA 841 GGGCGCGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGG AACCCCTATT TGTTTATTTT 901 TCTAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA ATGCTTCAAT 961 AATATTGAAA AAAGGAAGAG TATGAAGTAT TTCAACATTT TCCGGTGTCG CCCTTTATTT 1021 CCCTTTTTTT GCGGGCATTT TTGCCTTTCC CTGGTTATTT GCCTCAACCC AGAAACGGCT 1081 GGGTGGAAAG TTAAAAGATG CCTGAAGATC AAGTTGGGTT GCCACGAGGT GGGGTACATC 1141 GAACCTGGAT CTCAACAGCG GAAGAAATTC CTTGGAGAGA GAGTTTTTTT TCGGC // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Shimozono, S., Engineering FRET constructs using CFP and YFP. Methods Cell Biol. 85: 381-393 (2008). PMID 18155471. [link to RRC of NBRP] |
---|
2023.08.18
GNP_filter3_RDBDEP_html_230702.pl