Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMV-3xFLAG-mGli3_del97G-HA

Expression vector of mouse mutant Gli3, one-base-pair deletion of 97G, tagged with N-terminal 3xFLAG and C-terminal HA. Though this clone was constructed based on the pEGFP-C1, GFP was removed.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Makino,S. et al. Sci. Rep., 6: 39608, 2016).
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Makino,S. et al. Sci. Rep., 6: 39608, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB15041_A7Eop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro, 3xFLAG, insert 5' (del97G) Sequence file: RDB15041_A7Eoa.seq
>D04539A5_A7Eo_1_CMV-Forword_G02_20_ABI24.ab1     1 AGTCAACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA    61 GCAGAGCTGG TTTAGTGAAC CGTCAGATCC GCTAGCACCA TGGACTACAA AGACCATGAC   121 GGTGATTATA AAGATCATGA CATCGATTAC AAGGATGACG ATGACAAGGA GGCCCAGGCC   181 CACAGCTCTA CGGCGACTGA GAGGAAGAAA GCTGAAAATT CCATTGGGAA ATGTCCCACG   241 AGAACAGATG TCAGCGAGAA GCCGTGGCCT CTAGTACCAC TTCCAATGAG GATGAAAGTC   301 CTGGACAGAT CTATCACCGA GAGAGAAGAA ACGCAATCAC TATGCAGCCT CAGAGTGTGC   361 AGGGTCTCAA CAAAATCAGT GAGGAGCCCT CGACGTCTAG TGATGAGAGG GCCTCGCTGA   421 TCAAGAAAGA GATCCATGGC TCTCTACCAC ATCTGGCGGA GCCCTCTCTC CCTTACCGTG   481 GGACTGTGTT TGCCATGGAT CCCCGGAATG GCTACATGGA GCCTCACTAC CACCCTCCTC   541 ATCTTTTCCC TGCCTTCCAT CCTCCTGTAC CAATTGATGC CAGACATCAT GAGGGCCGTT   601 ACCATTATGA TCCATCTCCT ATTCCTCCAT TACATGTGCC TTCTGCCTTA TCTAGTAGCC   661 CGACGTATCC AGACTTGCCC TTCATTAGGA TCTCCCCACA CCGTAATCCC ACTGCAGCCT   721 CAGAGTCCCC CTTCAGCCCC CCACACCCCT ACATCAACCC ATATATGGAC TACATCCGCT   781 CATTGCACAG CAGCCCATCC CTCTCCATGA TCTCTGCTGC CCGAGGGCTG AGCCCTACAG   841 ATGCTCCCCA TGCTGGAGTC AGCCCTGCGG AATACTATCA CCAGATGGCT CTGCTGACAG   901 GCCAGCGCAG CCCTTATGCA GACATCCTTC CCTCAGCTGC CACTGCTGGT GCACGGGCCA   961 TCCATATGGA GTACCCTTCA TGCCATGGAC AGCACCAGAT TTCCCAGCCC TAGCTGTCAG  1021 CTAGCCAGGC CGGAAGCGCA CACTGTCATA TCGCCACTGT CAGATCCATA GCCTTCCGAC 1081 ATTCGACCAT GATAGACATC CCCCTACTCT GGTTTAACAA TTCCCTCCAA T //
Primer: SV40polyA-R Region: HA, insert 3' Sequence file: RDB15041_A7Eob.seq
>D04539A5_A7Eo_1_SV40polyA-R_H02_23_ABI24.ab1     1 AAAAGTGGGA GTTTTAGCAG TAAACCTCTA CAAATGTGGT ATGGCTGATT ATGATCAGTT    61 ATCTAGATCC GGTGGATCCC GGGCCCGCGG TACCGTCGAC TGCAGAATTC CTATTAAGCG   121 TAATCTGGAA CATCGTATGG GTAGGCCTGC ATAACTGCAA GGAACTTGCT TTCTTCTGCA   181 AGGGAGGTCA GCAAAGAACT CATATCCCCG ATAGCCATGT TGGTGGTGCC CATGGATAGG   241 GATGGGAATG GGAGGGATGC CCGCGGAGTG GTGAGACGGG AGGAGCTGTG GGAAAGGTTC   301 TGAATAATAC TTGGGCTCAA GGCCCCTGAC ATTAGGCTGG TATGGTCCCC ATCATCTATG   361 ATGGCATCAA AATCAATCTG CACACCTTCT AGGTCATGAC TCTCAAAGCT GTCAACTGTG   421 CTTGTTACCT GGTTAGCACC TGGGGAGAGT AACTCAGAGT TTTCAGTGCC ATTCCCTTGG   481 AGCAGGCAGT TGGCATCTGA GAGGGAGAAC GAGCCAGCTT TCCTGTCTTG CTGACTGAAG   541 CCCATGGTTT GGTCATAGAA CTGACCGGAA TAATTCTGCA CGGTAGAGCA GAGCTGCTGG   601 GACTGACCTT GTGCCTCCAT TTTGATGCTG TTCACCCTGC TGCTCTGACT CATGTCACTG   661 AGCTGTCCTT GTTGCAGAGT GAGGTTGCCC CTTGGCATTG CCTGACGCCT GTTACCCCCA   721 TAGCTGGCAC AGGGCTGGTA GCCCCTGATA GCTGCCATGC TTGATGGCTG GCTGCTGTTG   781 TCCTGAGTCC CTGGTAGGCA GCTCTCTGTC CCTTGATAGA TGTTGATATG TGAGGTAGCA   841 CCAACCTGCC CTAGTACCTG TTGACCAGGG CGGCTGGGCC CCTGGTGTTG CATGCTGCCA   901 CTGAGTAGCT GATGGGCAAT GTATCCCTGT CCCATTTGAT CTTGGGTTTG CATGCCATTC   961 ACTGTGTTGC CAGCTAACTC ATTTGGTGCC ACGGGACAGG CTGAAATCTA GCAACCCCCA  1021 TTCTCTTGCA AGCTGGTGCG TTTAGCTGAC CCCTTGAAAT CCCTGTACAC AGCCATGATC  1081 TAGAGCACTG GAGTCATTGT CTGGCCTGTG GATCTGACTC GATCACTGCT GCTCAAGGGC 1141 TGATTCCATG //
Primer: SV40pro_ori_F Region: SV40pro_ori, NeoR_KanR Sequence file: RDB15041_A7Eoc.seq
>15041_15041_A9IbF_SV40pro_ori_F_F06_18_ABI24.ab1     1 NNNNNNNNNN NNNGCNGCCT CGNCCTCTGN NCTATTCNNG AAGTAGTGAG GAGGCTTTTT    61 TGGAGGCCTA GGCTTTTGCA AAGATCGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA   121 TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT   181 ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC   241 AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAAG   301 ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG   361 ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC   421 TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC   481 GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG   541 AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC   601 ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGAGCATG CCCGACGGCG   661 AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC   721 GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGANCGCTAT CANGACATAG   781 CGTTGGCTAC CCGTGATATT GCTGAAGANC TTGGCGGCGA ATGNGCTGAC CGCTTCCTCG   841 TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC NNTCTATCGC CTTCTTGNNG   901 AGTNCTTCTG ANCGGGNNTC NGGGGGTTCN AAATGANNGA NNNANCGANN NNNNANNNGN 961 NNTCNNNANN TTNNNATTNN NNNGNNNNNC NTNNNNNNNA NNNNNNG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl