RDB14995 - pEGFP-C1-human TBC1D10B

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pEGFP-C1-human TBC1D10B

Expression vector of human TBC1 domain family member 10B (TBC1D10B), fused with N-terminal EGFP, CMV promoter.

Catalog number	RDB14995
Resource name	pEGFP-C1-human TBC1D10B
Alternative name	FLJ13130 EPI64B
Clone info.	Expression vector of human TBC1 domain family member 10B (TBC1D10B), fused with N-terminal EGFP, CMV promoter.
Comment	Expression was confirmed by the depositor with fluorescence microscope.
Vector backbone	pEGFP-C1 (plasmid)
Size of vector backbone	4.7 kb
Selectable markers	Kan^r (E. coli), Neo^r (mammalian cells).
Gene/insert name	human TBC1D10B cDNA
Depositor\|Developer	Fukuda, Mitsunori \|

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human TBC1D10B

External Database
human TBC1D10B

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Itoh, T. and Fukuda, M., J. Biol. Chem., 281(42): 31823-31831, 2006)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14995	pEGFP-C1-human TBC1D10B	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Original reference

original	Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14995_A7Egp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 Sequence file: RDB14995_A7Ega.seq
>D04556C7_A7Eg_2_EGFP-C2_A07_01_ABI24.ab1 1 TTCCCAACGA GAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC 61 TCTCGGCATG GACGAGCTGT ACAAGTCCGG ACTCAGATCC ATGTCTGGGA CCTTGGAGTC 121 CTTGGCGGAT GATGTGAGCT CCATGGGCTC AGATTCAGAG ATAAACGGGC TGGCCCTGCG 181 CAAGACGGAC AAGTATGGCT TCCTTGGGGG CAGCCAGTAC TCGGGCAGCC TAGAGAGCTC 241 CATTCCCGTG GACGTGGCTC GGCAGCGGGA GCTCAGATGG CTGGACATGT TCAGTAACTG 301 GGATAAGTGG CTGTCACGGC GATTCCAGAA GGTGAAGCTG CGCTGCCGGA AGGGGATCCC 361 CTCCTCTCTC AGAGCCAAAG CCTGGCAGTA CCTGTCTAAT AGCAAGGAAC TTCTGGAGCA 421 GAACCCAGGA AAGTTTGAGG AGCTGGAACG GGCTCCTGGG GACCCCAAGT GGCTGGATGT 481 GATTGAGAAG GACCTGCACC GCCAGTTCCC TTTCCACGAG ATGTTTGCTG CTCGAGGGGG 541 GCATGGGCAA CAGGACCTGT ACCGAATCCT GAAGGCCTAC ACCATCTACC GGCCTGACGA 601 GGGTTACTGC CAGGCCCAGG CCCCCGTGGC TGCGGTCCTG CTCATGCACA TGCCTGCGGA 661 GCAAGCCTTT TGGTGCCTGG TGCAGATCTG CGACAAGTAC CTCCCAGGTT ACTACAGTGC 721 AGGGCTGGAG GCCATTCAGC TGGACGGGGA GATCTTTTTT GCACTCCTGC GCCGGGCCTC 781 CCCGCTGGCG CATCGCCACC TGCGGCGGCA GCGCATTGAC CCTGTGCTCT ACATGACGGA 841 GTGGTTCATG TGCATCTTCG CCCGCACCCT GCCCTGGGCG TCGGTGCTGC GTGTCTGGGA 901 CATGTTTTTT CTGTGAAAGG CGTTAAGATC ATCTTCCGGG TGGCCCCTGG GTCCTGCTGC 961 GCCACACGCT GGGCTCAGTG GAGAAGCTGC GCTCCTGCCC AAGGCATGTA TGAGACCATG 1021 GAGCCAGCCT GCGTAACCTG CCCCAGCAGT GCCATGGCAA GAGGACTTCT GCTGCATGAG 1081 TGACCGATCT GGCGGATGAC CGAAGGCAAC TGAATTGGAA GCCGGA //
Primer: SV40polyA-R Sequence file: RDB14995_A7Egb.seq
>D04556C7_A7Eg_2_SV40polyA-R_A08_02_ABI24.ab1 1 GGGAATGCAG TTTTTAGCAG TAAACCTCTA CAATGTGGTA TGGCTGATTA TGATCAGTTA 61 TCTAGATCCG GTGGATCCCG GGCCCGCGGT ACCGTCGACC TGCAGGCGGC CGCGAATTCA 121 CTAGTGATTA GGGCAGAGGT CAGAAGTAAG CGTCCTGCCG GGCCTCGGCT GAGGGCCTGT 181 CCCCACCATC ATGGGGGCCT GGGGGCCCAT CTGCCTTTCG ACGCAGCGAA AGCTTCCGGC 241 CTTGAGCCTT CTTCTCCTGC TTCTGCCGCT CCTTCTCCTG CTTCTCTCGC TCCTTTTCCT 301 GCTTCTCTCG CTCTTTCTCC TGCTTCTGCC GCTCCTTCTC CCGCTCCTTC TCCTGTTTCT 361 GCCGCTCCTT CTCCTGTTTC TGCCGCTCCT TCTCCTGCTT CCGGGTCTCC TTGCTGGAAC 421 CCAAGGGGGT GCTATTGCCA GTGGGTGAGG GAAGGGATGG ATGCAGTCCC TCAGCAGTGA 481 CCACAGGCCC TGGGGCAGAC CCAGCACTGG CTCTGCGGAC GGGGGGCGGC GGGGACGGGG 541 CCCCTCCAGC TGCCCGGGAG TCTCGGCTCT TGAGGCCAGG GAGGCTGAGG AGGCTGGAGG 601 AGGGGCCCAG GGGTGGCTGT TGCCGCCGGC GCTCCTCGTG GATGGCCCGG GACCCATGCA 661 GTCGCCGTGA GGGCCGATAC TGCAGCTCCC CCCGCGTTTC CCGCCACTTC TTGAGCTGGG 721 CTGCATTCTC CCGCTCAATC AGTGCTTCTG TCACCGGCAG ATTGGTCACC TCATGCACCA 781 GGAAGTCTTC CTGCATGCAC TGCTGGGGCA GGTTACGCAG CTGCTCCATG GTCTCATACA 841 TGCCTTGGCA GGAGCGCAGC TTCTCCACTG AGCCCAGCGT GTGGCGCAGC AGGACCAGGG 901 CCACCCGGGA AGATGATCTT AACGCCTTCA CAGAAAAACA TGTCCCAGAC ACGCAGCACC 961 GACGCCCAGG GCAGGGTGCG GGCGAAGATG CACATGAACC CACTCCCGTC ATGTAGAGCA 1021 CAGGTCAATG CGGCTGCCGG CGCAGTGGCG ATGCGCAGCC GGGGAGCCCG CGCAGAGTGC 1081 AAAAGATCTC CCGTCAGCTG AATTGGCATC CAAGAGCCCT GGTA //
Primer: SV40pro_F_V2 Sequence file: RDB14995_A7Egc.seq
>D04556C7_A7Eg_2_SV40pro_F_V2_A09_03_ABI24.ab1 1 GTACGACTTT TTTGGAGCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT 61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC 121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC 181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG 241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG 301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG 361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG 421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC 481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG 541 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC 601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG 661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC 721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC 781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC 841 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGGTTCGA AATGACCGAC CAAGCGACGC 901 CCAACCTGCC ATCACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAGGT TGGGCTTCGG 961 AATCGTTTTC CCGGGACGCC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGGA 1021 GGTCTTCGCC CCACCCTAGG GGGAGGGCTA CCTGAAACAC GGGAGAAGAA CATACAGAAG 1081 AACCGCGCTA TGAACGGCAT AAAAAGGAAC CG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Itoh, T., Identification of EPI64 as a GTPase-activating protein specific for Rab27A. J. Biol. Chem. 281 (42): 31823-31831 (2006). PMID 16923811.
reference	Itoh, T., Screening for target Rabs of TBC (Tre-2/Bub2/Cdc16) domain-containing proteins based on their Rab-binding activity. Genes Cells 11 (9): 1023-1037 (2006). PMID 16923123.

2023.04.10

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