Please review the QC test results indicated by icon below as well as clone information before placing your order.

T7-NLS-hCAS9-NLS-P2A-EGFP-pA

Cas9-poly(A) with GFP to visualize Cas9 positive cells.

Sequence (full)	RDB14657zzt02.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. The RECIPIENT agrees to expressly describe the acknowledgement of the RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
Remarks	The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースを使用して研究成果を発表する場合、理研BRCから提供されたことを明示する。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
備考	本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14657_A7Csp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward () Sequence file: RDB14657_A7Csa.seq
>D04203A6_A7Cs_1_CMV-Forward_E10_13_ABI24.ab1     1 AGTTCACTCC GGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA    61 AGCAGAGCTC GTTTAGTGAA CCGTCAGATC GCCTGGAGAC GCCATCCACG CTGTTTTGAC   121 CTCCATAGAA GACACCGGGA CCGATCCAGC CTCCGGACTC TAGTAATACG ACTCACTATA   181 GGGAGAGCCG CCACCATGGC CCCAAAGAAG AAGCGGAAGG TCGGTATCCA CGGAGTCCCA   241 GCAGCCGACA AGAAGTACTC CATTGGGCTC GATATCGGCA CAAACAGCGT CGGCTGGGCC   301 GTCATTACGG ACGAGTACAA GGTGCCGAGC AAAAAATTCA AAGTTCTGGG CAATACCGAT   361 CGCCACAGCA TAAAGAAGAA CCTCATTGGC GCCCTCCTGT TCGACTCCGG GGAGACGGCC   421 GAAGCCACGC GGCTCAAAAG AACAGCACGG CGCAGATATA CCCGCAGAAA GAATCGGATC   481 TGCTACCTGC AGGAGATCTT TAGTAATGAG ATGGCTAAGG TGGATGACTC TTTCTTCCAT   541 AGGCTGGAGG AGTCCTTTTT GGTGGAGGAG GATAAAAAGC ACGAGCGCCA CCCAATCTTT   601 GGCAATATCG TGGACGAGGT GGCGTACCAT GAAAAGTACC CAACCATATA TCATCTGAGG   661 AAGAAGCTTG TAGACAGTAC TGATAAGGCT GACTTGCGGT TGATCTATCT CGCGCTGGCG   721 CATATGATCA AATTTCGGGG ACACTTCCTC ATCGAGGGGG ACCTGAACCC AGACAACAGC   781 GATGTCGACA AACTCTTTAT CCAACTGGTT CAGACTTACA ATCAGCTTTT CGAAGAGAAC   841 CCGATCAACG CATCCGGAGT TGACGCCCAA GCAATCCTGA GCGCTAGGCT GTCCAAATCC   901 CGGCGGCTCG AAAACCTCAT CGCACAGCTC CCTGGGGAGA AGAAGAACGG GCCCTGTTTG   961 GGTAAATCTT TATCGCCCTG TCACTCGGGC TGACCCCCCA ACTTTAAATC TAACCTTCGA  1021 CTGCCGAGAT GGCTAAGCCT TCCAACTGAA GCCAAAGACA CTACGATGAT GGATTCTCGA 1081 CCATCCTGCC TGGGTCA //
Primer: EGFP-N () Sequence file: RDB14657_A7Csb.seq
>D04203A6_A7Cs_1_EGFP-N_F10_16_ABI24.ab1     1 GTTTACCGGG GGGACAGCTC CTCGCCCTTG CTCACCATGG GGCCGGGGTT CTCCTCCACG    61 TCGCCGGCCT GCTTCAGCAG GGAGAAGTTG GTGGCCACCT TCCTCTTCTT CTTGGGGTCA   121 GCCCTGCTGT CTCCACCGAG CTGAGAGAGG TCGATTCTTG TTTCATAGAG CCCCGTAATT   181 GACTGATGAA TCAGTGTGGC GTCCAGGACC TCCTTTGTAG AGGTGTACCG CTTTCTGTCT   241 ATGGTGGTGT CGAAGTACTT GAAGGCTGCA GGCGCGCCCA AGTTGGTCAG AGTAAACAAG   301 TGGATAATGT TTTCTGCCTG CTCCCTGATG GGCTTATCCC TGTGCTTATT GTAAGCAGAA   361 AGCACCTTAT CGAGGTTAGC GTCGGCGAGG ATCACTCTTT TGGAGAATTC GCTTATTTGC   421 TCGATGATCT CATCAAGGTA GTGTTTGTGT TGTTCCACGA ACAGCTGCTT CTGCTCATTA   481 TCTTCGGGAG ACCCTTTGAG CTTTTCATAG TGGCTGGCCA GATACAAGAA ATTAACGTAT   541 TTAGAGGGCA GTGCCAGCTC GTTACCTTTC TGCAGCTCGC CCGCACTAGC GAGCATTCGT   601 TTCCGGCCGT TTTCAAGCTC AAAGAGAGAG TACTTGGGAA GCTTAATGAT GAGGTCTTTT   661 TTGACCTCTT TATATCCTTT CGCCTCGAGA AAGTCGATGG GGTTTTTTTC GAAGCTTGAT   721 CGCTCCATGA TTGTGATGCC CAGCAGTTCC TTGACGCTTT TGAGTTTTTT AGACTTCCCT   781 TTCTCCACTT TGGCCACAAC CAGTACACTG TAAGCGACTG TAGGAGAATC GAATCCGCCG   841 TATTTCTTGG GGTCCCAATC TTTTTTTGCG TGCGATCAGC TTGTCGCTGT TCCTTTTCGG   901 GAGGATACTT TCCTTGGAGA AGCCCTCCGG GTCTGTACTT CGGTCTTTTT AACGATGTTC   961 ACCTGCGGGC ATGGACAGGG ACCCTTCCCG GACTGTCGCG AAATCCCTAC CCTTGTCCCC  1021 AACACGAATT TCTCTTGTTT CTCCGTTTGT TTCGATAGTT GGATCGGCTT TCGATTCCTC 1081 ATGCCAGTGT AAATCTCGGT CTTGGAAAAA AAT //
Primer: EGFP-C2 () Sequence file: RDB14657_A7Csc.seq
>D04203A6_A7Cs_1_EGFP-C2_G10_19_ABI24.ab1     1 CTTCCAACGA GAGCGCGATC ACATGGTCCT GCTGGAGTTC GTGACCGCCG CCGGGATCAC    61 TCTCGGCATG GACGAGCTGT ACAAGTGAAA GGGTTCGATC CCTACCGGTT AGTAATGAGT   121 TTAAACGGGG GAGGCTAACT GAAACACGGA AGGAGACAAT ACCGGAAGGA ACCCGCGCTA   181 TGACGGCAAT AAAAAGACAG AATAAAACGC ACGGAAAAAA AAAAAAAAAA AAAAAAAAAA   241 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAGGCGCTA   301 GCTTTAACTT CGATCCCCCC CCGAAACCCA TTGGGGCCAA AACGCCCGCG TTTCTTCCTT   361 TTCCCCACCC CCCCCCCCAA TTCGGGTGAA GGCCCAGGGC TCGCAACCAA AGTTGGGGGG   421 GAGGGCCGGC CAAACAAATC GGCACACTGG GGTTCAAGGG GAACCCCCCC GCCCCTTGAC   481 GGCCATTACC GCGGGGGGGG GGGGTTACCC CACACTGGAC GTCACTTGGC AAGGCCCTAA   541 CGCCCCCCCC TTTGGTTTCT TCCCTCCTTT CGCCCATTTC CGGGTTTCCG TAAGTTTAAT   601 CGGGGCCCTT TAGGGTCCAA ATAGGCTTAG GCCTCACCCA AAAATTATAG GGAGGTCCTA   661 TGGCACTCCC TGAAACGTTC CCTTACTGGA CCCTTTTAAA GGACTGTTCA AGGAAACCCC   721 ACTCGGCATT TGATAAGGAT TCCATCCCTG GGAAAGCGTT CAATTCCAAT TTGGAGGGCA 781 AAGGGAATCC TTCCCAGAGA TTTGAGGGGT CTCTTACACC CGTTGAGCCC CCCC //
Primer: SV40pro_F () Sequence file: RDB14657_A7Csd.seq
>D04203A6_A7Cs_1_SV40pro_F_H01_08_ABI08.ab1     1 CTCTGCCCCT GGCCAATCAG AGGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA    61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT   121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA   181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC   241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA   301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG   361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC   421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG   481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA   541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC   601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA   661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG   721 TGGAAAATGG CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT   781 ATCAGGACAT AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG   841 ACCGCTTCCT CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC   901 GCCTTCTTGA CGAGTTTCTT CTGAGCGGGA CTCTGGGGTT CGCGAAATGA CCGACCAAGC   961 GACGCCCAAC CTGCCATCAC GAGATTTCGA TTCACGCGCT TTCTATGGAA AGTTGGCCTT  1021 CGGATTCGGT TTCGGGACGC GGCTGATGGA TTCTCAGCGC GGGAATCTCA ATGCTGAGTC 1081 TCGCCACCTA CTTGGTTATT GCCAGCCA //
Primer: HSV_TK_pA_R () Sequence file: RDB14657_A7Cse.seq
>14657_14657_A9G1F_1_HSV_TK_pA_R_E07_05_ABI08.ab1     1 CGGGGTCTTT TCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCG TTTAAACTCA    61 TTACTAACCG GTAGGGATCG AACCCTTTCA CTTGTACAGC TCGTCCATGC CGAGAGTGAT   121 CCCGGCGGCG GTCACGAACT CCAGCAGGAC CATGTGATCG CGCTTCTCGT TGGGGTCTTT   181 GCTCAGGGCG GACTGGGTGC TCAGGTAGTG GTTGTCGGGC AGCAGCACGG GGCCGTCGCC   241 GATGGGGGTG TTCTGCTGGT AGTGGTCGGC GAGCTGCACG CTGCCGTCCT CGATGTTGTG   301 GCGGATCTTG AAGTTCACCT TGATGCCGTT CTTCTGCTTG TCGGCCATGA TATAGACGTT   361 GTGGCTGTTG TAGTTGTACT CCAGCTTGTG CCCCAGGATG TTGCCGTCCT CCTTGAAGTC   421 GATGCCCTTC AGCTCGATGC GGTTCACCAG GGTGTCGCCC TCGAACTTCA CCTCGGCGCG   481 GGTCTTGTAG TTGCCGTCGT CCTTGAAGAA GATGGTGCGC TCCTGGACGT AGCCTTCGGG   541 CATGGCGGAC TTGAAGAAGT CGTGCTGCTT CATGTGGTCG GGGTAGCGGC TGAAGCACTG   601 CACGCCGTAG GTCAGGGTGG TCACGAGGGT GGGCCAGGGC ACGGGCAGCT TGCCGGTGGT   661 GCAGATGAAC TTCAGGGTCA GCTTGCCGTA GGTGGCATCG CCCTCGCCCT CGCCGGACAC   721 GCTGAACTTG TGGCCGTTTA CGTCGCCGTC CAGCTCGACC AGGATGGGCA CCACCCCGGT   781 GAACAGCTCC TCGCCCTTGC TCACCATGGG GCCGGGGTTC TCCTCCACGT CGCCGGCCTG   841 CTTCAGCAGG GAGAAGTTGG TGGCCACCTT CCTCTTCTTC TTGGGGTCAG CCCTGCTGTC   901 TCCACCGAGC TGAGAGAGGT CGATTCTTGT TTCATAGAGC CCCGTTAATT GACTGATGAA   961 TCAGTGTGGC GTCCAGGACC CTCCTTTGTA GAAGGTGTAC CCGCTTTCTG TCTATGGGTG  1021 GGTGTCGAAG TACTTGGAAA GGCTGCAAGC GCGCCCAAGG TTGGTCAAGA GAGTAAACAA  1081 GTGATAATTG TTTCCTGCTT GCTCCTGAAT TGGCCTATCC TGTGCCTATG GTTAAGGCCA 1141 GGAAAAGGGC AACCCTTA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl