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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1-hTRPV1-FLAG WT

Expression vector human Transient receptor potential vanilloid type I (TRPV1) wild type with C-terminal FLAG-tag.

Catalog number RDB14587
Resource name pcDNA3.1-hTRPV1-FLAG WT
Clone info. Expression vector human Transient receptor potential vanilloid type I (TRPV1) wild type with C-terminal FLAG-tag.
Comment Expression was confirmed by the depositor with Ca2+ flux assay.
Vector backbone pcDNA3.1(+) (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name human TRPV1 cDNA
Depositor|Developer Ohbuchi, Katsuya |
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External Database
human TRPV1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ohbuchi, K. et al. PLoS One., 11 (9): e0162543, 2016)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ohbuchi, K. et al. PLoS One., 11 (9): e0162543, 2016)。
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          RDB14587 pcDNA3.1-hTRPV1-FLAG WT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3.1-hTRPV1-FLAG WT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14587).

          Reference section:

          Ohbuchi, K., Mori, Y., Ogawa, K., Warabi, E., Yamamoto, M., Hirokawa, T., Detailed Analysis of the Binding Mode of Vanilloids to Transient Receptor Potential Vanilloid Type I (TRPV1) by a Mutational and Computational Study. PLoS One 11 (9): e0162543 (2016). PMID 27606946. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14587_A7B9p1-3.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,T7 pro,insert 5'(hTRPV1 WT 5')
          Sequence file: RDB14587_A7B9e.seq check
          >D04124A1_A7B9_1_CMV-Forward_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGNNGCN NTGGGCGGTA NGCGTGTNCG GTGGGNGGTC
             61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
            121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGCCACCA
            181 TGAAGAAATG GAGCAGCACA GACTTGGGGG CAGCTGCGGA CCCACTCCAA AAGGACACCT
            241 GCCCAGACCC CCTGGATGGA GACCCTAACT CCAGGCCACC TCCAGCCAAG CCCCAGCTCT
            301 CCACGGCCAA GAGCCGCACC CGGCTCTTTG GGAAGGGTGA CTCGGAGGAG GCTTTCCCGG
            361 TGGATTGCCC TCACGAGGAA GGTGAGCTGG ACTCCTGCCC GACCATCACA GTCAGCCCTG
            421 TTATCACCAT CCAGAGGCCA GGAGACGGCC CCACCGGTGC CAGGCTGCTG TCCCAGGACT
            481 CTGTCGCCGC CAGCACCGAG AAGACCCTCA GGCTCTATGA TCGCAGGAGT ATCTTTGAAG
            541 CCGTTGCTCA GAATAACTGC CAGGATCTGG AGAGCCTGCT GCTCTTCCTG CAGAAGAGCA
            601 AGAAGCACCT CACAGACAAC GAGTTCAAAG ACCCTGAGAC AGGGAAGACC TGTCTGCTGA
            661 AAGCCATGCT CAACCTGCAC GACGGACAGA ACACCACCAT CCCCCTGCTC CTGGAGATCG
            721 CGCGGCAAAC GGACAGCCTG AANGAGCTTG TCAACGCCAG CTACACGGAC AGCTACTACA
            781 AGGGCCAGAC AGCACTGCAC ATCGCCATCG ANAGACGCAA CATGGCCCTG GNGACCCTCC
            841 TGGTGGANAA CGGANCAGAC GTCCAGGCTG CGGNCNNNNG GGGANTNCTT TAANAAAACN
          901 AAANGGNCGG NCNNNNNTCT ACTTCNNNGN NNNGNCCCNN NNNCCNNNNN NNNNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH pA,insert 3'(FLAG,hTRPV1 WT 3')
          Sequence file: RDB14587_A7B9f.seq check
          >D04124A1_A7B9_1_BGH_rev2_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGGGGGNNGG GCANCNACAG NTGGCTGNNN NNNAGAAGGC
             61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCTTACTT
            121 GTCGTCATCG TCTTTGTAGT CCTTCTCCCC GGAAGCGGCA GGACTCTTGA AGACCTCAGC
            181 GTCCTCTGGC TTCAGAGACC CTGAAAACTG TCGCAGATAA ACTTCCTCGG GCTGAGCAGA
            241 CTGCCTATCT CGAGCACTTG CCTCTCTTAA AAGGGGGACC AGGGCAAAGT TCTTCCAGTG
            301 TCTGCCTGAA ACTCTGCTTG ACCGCAGGGA GAAGCTCAGG GTGCGCTTGA CGCCCTCACA
            361 GTTGCCCGGG TCTTCGTTGA TGATGCCCAC GTTGGTGTTC CAGGTGGTCC AGTTCACCTC
            421 GTCCACCCTG AAGCACCACC GGTAGTCGTC CTTGCCATCA GGTGTGTACC CCACCTGCAG
            481 CAGCTTGCCT GAGCGGAAGG CCTTCCTCAT GCACTTAAGG AAGCTCTTCT CCGTGTCCAG
            541 GATGGTGATG GCTCTCTGCA GCTTCCAGAT GTTCTTGCTC TCCTGTGCGA TCTTGTTGAC
            601 AGTCTCACCC ATGAGGGCGA TGAGCATGTT GAGCAGGAGG ATGTAGGTGA GAATTACATA
            661 GGCCAGCAGC AGGATGATGA AGACAGCCTT GAAGTCATAG TTCTCAGTGA ACTCCAGGTC
            721 GCCCATGCCG ATGGTGAACT TGAACAGCTC CAGGCAGGTG GAGTACAGGC TGTTGTANGA
            781 NCTATCGGGG GGGCCTGCAG GCAGGCCCCC GCCACCTGNG CGANGTGGAC TCAGACGGCA
            841 NGGANTCATT CNNNCNGNCT TCAATCANCG TCACCANNGC NNNGGAAAAC CCGANNNNNA
            901 AGANNANGNA GNNAAACATG AANCNGCNNA NGNNCTCNCN NNATNATCNT NNNNNATCNT
          961 GNNGNNNNNN NNNN
          //
          Primer: RDB14587#1 (Pr0440)
          Region: insert mid(hTRPV1 WT mid)
          Sequence file: RDB14587_A7B9g.seq check
          >D04124A1_A7B9_1_RDB14587#1_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NCNNGNNGNN GTAGGTGANA ATTACNTAGG CCAGCAGCAG
             61 GATGATGAAG ACAGCCTTGA AGTCATAGTT CTCAGTGAAC TCCAGGTCGC CCATGCCGAT
            121 GGTGAACTTG AACAGCTCCA GGCAGGTGGA GTACAGGCTG TTGTAGGAGC TATCGGGGGG
            181 CCTGCAGGCA GGCCCCCGCC ACCTGTGCGA CGTGGACTCA GACGGCAGGG AGTCATTCTT
            241 CCCGTCTTCA ATCAGCGTCA CCACCGCTGT GGAAAACCCG AACAAGAAGA CGATGTAGAC
            301 AAACATGAAA CGGCACAGGT CTCTCAGGAT CATCTTCTCT ATCATGACGG CATAGATGCC
            361 CATCTGCTGG AAACCGCGGG TGTAGTAGAG CATGTTGGTC CAGCCCAAGG CCAGGGAGAA
            421 TACCATGGAA GCCACATACT CCTTGAGGTG GCTGAAGTAC AGCACCACGG TGGCCAGCAT
            481 GAACAGTGAC TGCAGAAAGA AAAGCATCTC ACTGTAGCTG TCCACAAACA GGGTCTTCAT
            541 CGACGGCCGC CTCTGCAGGA AATACTGAAT CCCTCGGAAA AAGAAGTAGA CTCCTCCTAA
            601 CACAGACAGG ATCTCTCCAG TAACTCGGAA ATAGTCTCCA GTTTTTTCCA TCTTAAAGGG
            661 AGGCAAGCCA TCCACGGGCC TGTAGTAGGC AGCCATGGTG AAGATGATCA TGTACAGGCA
            721 GTAGACCAGG AAGTTGAAGT AGAAGATGCG CTTGACGAAT CTGTCCCACT TGTCCTGCAG
            781 GANTCGGTTC AGCGGCTCCA CCAAGANCAT GTCGNGGCGA TTANGGGTCT CGCTGCTGCT
            841 GTANGCGATC ACCTCCNGCA NCNANTTCTT CTCGCANGNG NCNANGCNNN ACAGGNCGNA
            901 CANCNANGNN NGNNCGGGNC NNNNNNCCNN NNNNGNNNNC TTNCNNNNNA GNNGNCNTGC
          961 NNNNNGNNNN NNNNNN
          //
          Primer: SV40pro_F (Pr0110)
          Region: NeoR
          Sequence file: RDB14587_A7B9h.seq check
          >D04124A1_A7B9_1_SV40pro_F_D01_10_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGNNNNNNTT TTTTNNGNNG
             61 GCCTAGGNCT TTTNNNNNNN NNCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA
            121 AGAGACAGGA TGAGGATCGT TTCNCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC
            181 GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC
            241 TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA
            301 CCTGTCCGGT GCCCTGAATG AACTGCAGGA CNANGCAGCG CGGCTATCGT GGCTGGCCAC
            361 GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT
            421 GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA
            481 AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC
            541 ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT
            601 TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC
            661 CAGGCTCAAG GCGCGCATGC NNNNNGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG
            721 CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TNCNTCGACT GTGGNCGGCT
            781 GGGGTGTGGC GGANCGCTAT CANGACATAG CGTNNGCTAC CCGTGATATT GCTGAAGANC
            841 TTGGNNGGCG NANGGGGCTG ANCGCTTCCT CNNGCTTNAN GGTATCGCCG CTCCCGATTN
            901 NNNNNGCATC GCCNTCNATC GCCTTCTTGA CGANTNNNNC TGANCGGGNN TCNNGGGGNT
          961 CNNNNGACNN ACCNNGCNNA NNNCNNNNNT GNNNNNNNNN ANNNTNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ohbuchi, K., Detailed Analysis of the Binding Mode of Vanilloids to Transient Receptor Potential Vanilloid Type I (TRPV1) by a Mutational and Computational Study. PLoS One 11 (9): e0162543 (2016). PMID 27606946. [link to RRC of NBRP]

          2024.03.26

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