RDB14516 - pGL4_ifng-2000-luc

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pGL4_ifng-2000-luc

Reporter construct of mouse interferon gamma (Ifng) promoter (-2000 to +113).

Catalog number	RDB14516
Resource name	pGL4_ifng-2000-luc
Clone info.	Expression vector of luciferase2CP under the mouse interferon gamma (Ifng) promoter (-2000 to +113) for reporter assay. The structure of the reporter construct, see Fig. 2B of Fukuoka, N. et al. Genes Cells, 21 (2): 146-162, 2016.
Comment	The transcription start site was deduced from the sequences conserved between the human, rat, and mouse IFN-gamma genes reported by Penix et al., J. Exp. Med., 178, 1483-1496 (1993). The first exon of the mouse IFN-gamma gene has been revised in NCBI Reference Sequence: NM_008337.4. In pGL4_ifng-2000-luc, the PCR product (2.1 kb) was amplified with forward primer (5' CTA CTC GAG ATT GCT GGC TTC TGT CAC CTG 3') and reverse primer (5' CTA GAT ATC TGT CTC AGA GCT AGG CCG CAG GAG GAG AAG C 3'), digested with Xho I and EcoRV, and inserted into the Xho I and EcoRV sites of pGL4.22[luc2CP/Puro] vector. Genomic DNA fragment 118,439,039 to 118,441,154 nt of NC_000076.6 is cloned. Known differences (at least): A deletion at 118,441,150 nt; A insertion between 118,440,965 and 118,440,966 nt.
Vector backbone	pGL4.22[luc2CP/Puro] (Plasmid)
Selectable markers	Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name	mouse Ifng genomic DNA
Depositor\|Developer	Kataoka, Takao \|

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mouse Ifng

External Database
mouse Ifng

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)

Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)。
付加的提供条件	本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14516	pGL4_ifng-2000-luc	DNA solution

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References and tips

Electronic file

Original reference

original	Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212.

Further references such as user reports and related articles (go to bottom)

Featured content	T cell receptor signaling pathway (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14516_A6Lfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL4-4174F Region: insert 5' Sequence file: RDB14516_A6Lfa.seq
>D03972A2_A6Lf_1_pGL4-4174F_D07_04_ABI08.ab1 1 ATAGGGGTCA GACATTTCTC TGGCCTAACT GGCCGGTACC TGAGCTCGCT AGCCTCGAGA 61 TTGCTGGCTT CTGTCACCTG AGTGCTGGGA TTAAAGGTAT GAGTTACCAC GACCCTGCTA 121 GATTTTTTTT TTAAGACAGA TTCTCATGAT TCTTAAACTG GCCTTCAAGT CTCCAGCAGC 181 AGAAGGTGAC CTCGCACTCA TGATCATCTT ACCTCTGTTT CCATCTGTAT TTCATGCCTA 241 TTTTAAGTCA GGGTTTTTAT ACAAGCACTC TACCAACCAA GAGATGTTCC TGACTGTCAA 301 AACAGAGATT CTTAGGTAAA CATTCAAATG AATATTTGTG TGTGTGTGTG TGTTGTTTGT 361 GTATAAATCT TTATGCTTAT GTATTTGAGG GAAATAAATT GTGTCACAGA AGCAATGAAA 421 CACTTTCTGG TTTTAAGGAA ATTAGTGGGC ACTGGGATGG GGGTAGGGGG CATGCATAGT 481 TACCTCGTAT ATGAGACTGG GGAAATTGAG AACCCATGAA GCATAGAAAC AAAGTGCTAG 541 CCCTGCCCAG AGGATAAAGT CTGATTTGAA GCTGGAGGCT TGGGGGAGAA CCAAAACAGA 601 GGATGGTTTG CATCTGGGTC AAGATAACTG GGTACCACTC TGAGATGCTC AGATGAGGGA 661 AAGGGGGCAC ATGGCCAAAG GAACTGCACG AACAAGCTGC ATAGCGTTGT TAAACATCTG 721 ACATTGCCAA ATAACCATCA AGTTTTAATT GAGCCACTAG GAATGCCGGG AAGAATTCTC 781 TCAACTTTCC ATGGAGCATT TGGTTGAGCC TTTGGCTGAA GTCCGGAAGA AAATGCTAGT 841 GTTAAGCTCA CTGTATAAAT GTGGAAACTG AGTCACAGAT CATAAAGATG CTGACTCAAG 901 ACCCCGAGGC TAGAATATGA ATGGTTTCAA GTCTGCACCC ATAGCCACTG TGTAATGTTT 961 AATGAATACA GGAAGGCAGG GATGATACAC GTGGGCTGCA CTGGAGAGCA GAGGTCTCCT 1021 GCCCCAATCT TCACGAATCG GAATTTCCAT AGATAAGGTA CTAAGGATGA GCAGAACCTT 1081 TTATGGTGAC TTGATGCGAA GCAACTAT //
Primer: Luc_R Region: insert 3' Sequence file: RDB14516_A6Lfb.seq
>D03972A2_A6Lf_1_Luc_R_E07_05_ABI08.ab1 1 ACGAATGGTG CCGGGCACAG CGCGTAGCGC TTCATGGCTT TGTGCAGCTG CTCGCCGGCG 61 GTCCCGTCTT CGAGTGGGTA GAATGGCGCT GGGCCCTTCT TAATGTTTTT GGCATCTTCC 121 ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC CGCCGAGGCC AGATCTTGAT 181 ATCTGTCCAG AGCTAGGCCG CAGGAGGAGA AGCCCAGAAC TTCTGTCTCA AGTCAGAGGG 241 TCCAAAGGAT CAGCTGATGT GTCTTCTCTA GGTCAGCCGA TGGCAGCTAT AGCCTGCACC 301 TCTCTGGCTT CCAGTTTTAT ACCTGATCGA AGGCTCCTCG GGATTACGTA TTTTCACAAG 361 TTTTTTTTTG GTTTTTTTTT TTTTTTTAAG ATGGTGACAG ATAGGTGGGG GGAGCTAAGT 421 TACTTTGCAT TACAGCTATG GTTTTGTGGC ATGTTAGAGT TTCCTTTCGA CTCCTTGGGC 481 TCTCTGACGA TGAGACAGCC CCGCTGTGCC CACCTGTGCC ATTCTTGTGG GATTCTCTGA 541 AAGCACTTTT ACTTCACACC ATTTGGGGTG GGGGCTGGAA ATTGCTTTTA CGTCTCCCCC 601 TTTTTGCCCT TGTAATGTGA ATTTCTCATC CACAGAGCAC AGCACAGGGA GCCTTTGTTT 661 GTGGTTGGGG ACGGCTGAAT AAAAGGAAAC TCTTGGGCTT CTCAAACCAT GAGGAAACTC 721 TTGGGCTTCT CAAACCATGC AATGGGGGAA GCCTTTTCAA ACTTTTCTTG CAAATGACCA 781 GAAAAGCAAG GAAAGGGTAT GGTTAAAAAG CCAATGTGGT GAGGAAGCCC TTCAATGGAG 841 GTGATTAGTA AAATACCTAC TACAAATCAC TCAGGGTCAA CGTGCCCAGA AAGAAGAAAA 901 GGCAGACCAC ATAGGTAAAA TAAAATCATC TAGTTCCTCC ATTAATAAAA GGGAAAAAAA 961 TGCAAAATGA ATAAAAGTAA AAATGCCAAG ATACTTTCAT TTAGATATTG TATTTCACTC 1021 ACACACCCGA GTATCATGTG TATCAGGAAT TTATGATGGA TTAGAGTTCC ATTTCGTCTG 1081 CACTAGATTT AAGCCTAGTG CCTATTCCTA //
Primer: SV40pro_R2 Region: Luciferase 3' Sequence file: RDB14516_A6Lfc.seq
>D03972A2_A6Lf_1_SV40pro_R2_D09_12_ABI24.ab1 1 AAAAATCCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 61 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGAAGGTA GCTAACCAAG TTCCTCTTTC 121 AGAGGTTATT TCAGGCCATG GTGCTGCGCA GATCAGCGGA AGAGCGCCCA ATACGCAAAC 181 GGATCCTTAT CGATTTTACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC 241 ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG 301 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT 361 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGCT 421 CGAAGCGGCC GGCCGCCCCG ACTCTAGACT GCAGTTAGAC GTTGATCCTG GCGCTGGCGC 481 AAGCAGCAGG GTGTCTATCC ATGCCGCTCT CCTGGGCGCA GCTCATGGGC AGGGTGCCGG 541 CGGCCTGCTC CTCCACCTCG GGAGGGAAGC CGTGGCTGTT AAGGTGGATC ACAAAGTGGC 601 TTAAGCTACT GAACCAGTTC TTGCAAGCAG AATTCACGGC GATCTTGCCG CCCTTCTTGG 661 CCTTAATGAG AATCTCGCGG ATCTTGCGGG CGTCCAACTT GCCGGTCAGT CCTTTAGGCA 721 CCTCGTCCAC GAACACAACA CCACCGCGCA GCTTCTTGGC GGTTGTAACC TGGCTGGCCA 781 CATAGTCCAC GATCTCCTTC TCGGTCATGG TTTTACCGTG TTCCAGCACG ACGACTGCGG 841 CGGGCAGCTC GCCGGCATCG TCGTCGGGCA GGCCGGCGAC CCCGGCGTCG AAGATGTTGG 901 GGTGTTGCAG CAGATGCTCT CCAGTTCGGC TGGGGCTACC TGGTAGCCCT TGTATTTGAT 961 CAAGCTCTTC AGCCGGTCCA CGATGAAAGA AGTGCTCGTC CTCGTCCCAG TAGGCGATGT 1021 CGCCGCTGTG CAGCCAGCCC GTCCCTTGTC GATGAGAGCG TTTGTAGCCC TCGGGGATGT 1081 TAACGTAGCC GCCTCATGAA TCATGGGGCA CGAACGCCAC AGCTCGGACG CGCTGGTTCA 1141 ACCACCCAAG GTTGTCT //
Primer: SV40pro_F Region: SV40 pro/ori, PuroR, poly(A) signal Sequence file: RDB14516_A6Lfd.seq
>D03972A2_A6Lf_1_SV40pro_F_F07_06_ABI08.ab1 1 CATCCTCTTT AACAATCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 61 AAGCTCGATT CTTCTGACAC TAGCGCCACC ATGACCGAGT ACAAGCCTAC CGTGCGCCTG 121 GCCACTCGCG ATGATGTGCC CCGCGCCGTC CGCACTCTGG CCGCCGCTTT CGCCGACTAC 181 CCCGCTACCC GGCACACCGT GGACCCCGAC CGGCACATCG AGCGTGTGAC AGAGTTGCAG 241 GAGCTGTTCC TGACCCGCGT CGGGCTGGAC ATCGGCAAGG TGTGGGTAGC CGACGACGGC 301 GCGGCCGTGG CCGTGTGGAC TACCCCCGAG AGCGTTGAGG CCGGCGCCGT GTTCGCCGAG 361 ATCGGCCCCC GAATGGCCGA GCTGAGCGGC AGCCGCCTGG CCGCCCAGCA GCAAATGGAG 421 GGCCTGCTTG CCCCCCATCG TCCCAAGGAG CCTGCCTGGT TTCTGGCCAC TGTAGGAGTG 481 AGCCCCGACC ACCAGGGCAA GGGCTTGGGC AGCGCCGTCG TGTTGCCCGG CGTAGAGGCC 541 GCCGAACGCG CCGGTGTGCC CGCCTTTCTC GAAACAAGCG CACCAAGAAA CCTTCCATTC 601 TACGAGCGCC TGGGCTTCAC CGTGACCGCC GATGTCGAGG TGCCCGAGGG ACCTAGGACC 661 TGGTGTATGA CACGAAAACC TGGCGCCTAA TGATCTAGAA CCGGTCATGG CCGCAATAAA 721 ATATCTTTAT TTTCATTACA TCTGTGTGTT GGTTTTTTGT GTGTTCGAAC TAGATGCTGT 781 CGACCGATGC CCTTGAGAGC CTTCAACCCA GTCAGCTCCT TCCGGTGGGC GCGGGGCATG 841 ACTATCGTCG CCGCACTTAT GACTGTCTTC TTTATCATGC AACTCGTAGG ACAGGTGCCG 901 GCAGCGCTCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG 961 AGCGGTATCA GCTCACTCAA GGCGGTAATA CGGTTATCCA CAGAAATCAG GGGAATACGC 1021 AGAAGACAAT GTGAGCAAAT GTCAGCCAAA ATGCCAGGAA TCGTTAAAAG CGCGTATGGC 1081 TGGGCGATTT TTCCAAATAG GT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl