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pGL4_ifng-2500-luc

Reporter construct of mouse interferon gamma (Ifng) promoter (-2500 to +113).

Catalog number RDB14515
Resource name pGL4_ifng-2500-luc
Clone info. Expression vector of luciferase2CP under the mouse interferon gamma (Ifng) promoter (-2500 to +113) for reporter assay. The structure of the reporter construct, see Fig. 2B of Fukuoka, N. et al. Genes Cells, 21 (2): 146-162, 2016.
Comment The transcription start site was deduced from the sequences conserved between the human, rat, and mouse IFN-gamma genes reported by Penix et al., J. Exp. Med., 178, 1483-1496 (1993). The first exon of the mouse IFN-gamma gene has been revised in NCBI Reference Sequence: NM_008337.4. In pGL4_ifng-2500-luc, the PCR product (2.6 kb) was amplified with forward primer (5' CTA CTC GAG AAA TGG GAC ACA GAT CTC 3') and reverse primer (5' CTA GAT ATC TGT CTC AGA GCT AGG CCG CAG GAG GAG AAG C 3'), digested with Xho I and EcoRV, and inserted into the Xho I and EcoRV sites of pGL4.22[luc2CP/Puro] vector. Genomic DNA fragment 118,438,542 to 118,441,154 nt of NC_000076.6 is cloned. Known differences (at least): C deletion at 118,438,965 nt; A insertion between 118,440,965 and 118,440,966 nt.
Vector backbone pGL4.22[luc2CP/Puro] (Plasmid)
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name mouse Ifng genomic DNA
Depositor|Developer Kataoka, Takao |
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External Database
mouse Ifng

          Reference sequence
            

          Distribution information

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          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fukuoka, N. et al. Genes to Cells 21, 146-162, 2016)。
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          RDB14515 pGL4_ifng-2500-luc DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content T cell receptor signaling pathway (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14515_A6Lfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Region: insert 5'
          Sequence file: RDB14515_A6Lfa.seq check
          >D03972A1_A6Lf_1_pGL4-4174F_A07_01_ABI08.ab1
              1 GCGGGTCAGA CATTTCTCTG GCCTAACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGAAA
             61 TGGGACACAG ATCTCAACAG AGAATTCTCC ATAGTGAAAA GTCACATGGC TGAGAAACAC
            121 TTCAATGTTT AACATCCTTA GCCATTAGGG AAATGCAATT CAAAACTTCT CTGAGATCCC
            181 ATCTTACATC TGTCAGAATG TCTAAGATCA ATAAGACACG TGACAGCTCA CACTGGTGTG
            241 GAGCAAGGGG AACACTCTCC TACTGAGGTT GGTAGTGCAA ATATCACTTT TAAAAAATTA
            301 ATAGAGAACA TAGAACGGTC CCCGTCATCA GGTGTTAGCT AACGAGACCA CACCATTTTC
            361 TAGCATTAAT GGTAAATTAC ATTTAATAAA AAAGCTGATT TCCTTCAAAC TAGAAGGAAT
            421 AGCATTGGAA CTGTTAAAAA ACTTGAGGGC AATAACTCAA ACTAGATTCC CCCCCCCCCC
            481 AGAAAGGGTT TCACTGTGTA GCTCTGGTTA TCCTGGAACT CACTCTGTAG ACCAGGATAA
            541 CCTCAAATTC ACAGAGATTG CTGGCTTCTG TCACCTGAGT GCTGGGATTA AAGGTATGAG
            601 TTACCACGAC CCTGCTAGAT TTTTTTTTTA AGACAGATTC TCATGATTCT TAAACTGGCC
            661 TTCAAGTCTC CAGCAGCAGA AGGTGACCTC GCACTCATGA TCATCTTACC TCTGTTTCCA
            721 TCTGTATTTC ATGCCTATTT TAAGTCAGGG TTTTTATACA AGCACTCTAC CAACCAAGAG
            781 ATGTTCCTGA CTGTCAAAAC AGAGATTCTT AGGTAAACAT TCAAATGAAT ATTTGTGTGT
            841 GTGTGTGTGT TGTTTGTGTA TAAATCTTTA TGCTTATGTA TTTGAGGGAA ATAAATTGTG
            901 TCACAGAAAG CAATGAAACA CTTTTCTGGT TTTAAAGGAA ATTAGTGGGG CACTGGGATG
            961 GGGGGTAGGG GGGCATGCAT AGTTACCCTC GTATATGAGA CTGGGGGAAA TTTGAGAACC
           1021 CATGAAGGCA TAGAACAAAG TGCTAGCCCT GCCCAGAGGG ATAAAGTCTG ATTTGCAGGC
          1081 CTGGAGGCTG GGGGAGACTC AACAGAGGAT TGGATTTGCC AT
          //
          Primer: Luc_R
          Region: insert 3'
          Sequence file: RDB14515_A6Lfb.seq check
          >D03972A1_A6Lf_1_Luc_R_B07_02_ABI08.ab1
              1 AGTTAAAGGG TTCCGGGGCA CAGGGCGTAG CGCTTCATGG CTTTGTGCAG CTGCTCGCCG
             61 GCGGTCCCGT CTTCGAGTGG GTAGAATGGC GCTGGGCCCT TCTTAATGTT TTTGGCATCT
            121 TCCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT GGCCGCCGAG GCCAGATCTT
            181 GATATCTGTC TCAGAGCTAG GCCGCAGGAG GAGAAGCCCA GAACTTCTGT CTCAAGTCAG
            241 AGGGTCCAAA GGATCAGCTG ATGTGTCTTC TCTAGGTCAG CCGATGGCAG CTATAGCCTG
            301 CACCTCTCTG GCTTCCAGTT TTATACCTGA TCGAAGGCTC CTCGGGATTA CGTATTTTCA
            361 CAAGTTTTTT TTTGGTTTTT TTTTTTTTTT TAAGATGGTG ACAGATAGGT GGGGGGAGCT
            421 AAGTTACTTT GCATTACAGC TATGGTTTTG TGGCATGTTA GAGTTTCCTT TCGACTCCTT
            481 GGGCTCTCTG ACGATGAGAC AGCCCCGCTG TGCCCACCTG TGCCATTCTT GTGGGATTCT
            541 CTGAAAGCAC TTTTACTTCA CACCATTTGG GGTGGGGGCT GGAAATTGCT TTTACGTCTC
            601 CCCCTTTTTG CCCTTGTAAT GTGAATTTCT CATCCACAGA GCACAGCACA GGGAGCCTTT
            661 GTTTGTGGTT GGGGACGGCT GAATAAAAGG AAACTCTTGG GCTTCTCAAA CCATGAGGAA
            721 ACTCTTGGGC TTCTCAAACC ATGCAATGGG GGAAGCCTTT TCAAACTTTT CTTGCAAATG
            781 ACCAGAAAAG CAAGGAAAGG GTATGGTTAA AAAGCCAATG TGGTGAGGAA GCCCTTCAAT
            841 GGAGGTGATT AGTAAAATAC CTACTACAAA TCACTCAGGG GTCAACGTGC CCAGAAAGAA
            901 GAAAAGGCAG ACCACATAGG TAAAATAAAA TCATCTAGTT TCCTCCATTA ATAAAAGGGA
            961 AAAATGCAAA TGAATAAAAG TAAAAATGCA GAATACTTTC ATTAGATATT GTATTTCACT
           1021 TCAACACACC CAAGTATCAA TGTGTAATCC AGATTTATGG ATGAATAAGA GTTTTCAATT
          1081 CCTCTGGCAT TTAAGTATTT GAAGGTCCTA GATTTGC
          //
          Primer: SV40pro_R2
          Region: Luciferase 3'
          Sequence file: RDB14515_A6Lfc.seq check
          >D03972A1_A6Lf_1_SV40pro_R2_C09_09_ABI24.ab1
              1 TGTCAGGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG
             61 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGAAGGTA GCTAACCAAG TTCCTCTTTC
            121 AGAGGTTATT TCAGGCCATG GTGCTGCGCA GATCAGCGGA AGAGCGCCCA ATACGCAAAC
            181 GGATCCTTAT CGATTTTACC ACATTTGTAG AGGTTTTACT TGCTTTAAAA AACCTCCCAC
            241 ACCTCCCCCT GAACCTGAAA CATAAAATGA ATGCAATTGT TGTTGTTAAC TTGTTTATTG
            301 CAGCTTATAA TGGTTACAAA TAAAGCAATA GCATCACAAA TTTCACAAAT AAAGCATTTT
            361 TTTCACTGCA TTCTAGTTGT GGTTTGTCCA AACTCATCAA TGTATCTTAT CATGTCTGCT
            421 CGAAGCGGCC GGCCGCCCCG ACTCTAGACT GCAGTTAGAC GTTGATCCTG GCGCTGGCGC
            481 AAGCAGCAGG GTGTCTATCC ATGCCGCTCT CCTGGGCGCA GCTCATGGGC AGGGTGCCGG
            541 CGGCCTGCTC CTCCACCTCG GGAGGGAAGC CGTGGCTGTT AAGGTGGATC ACAAAGTGGC
            601 TTAAGCTACT GAACCAGTTC TTGCAAGCAG AATTCACGGC GATCTTGCCG CCCTTCTTGG
            661 CCTTAATGAG AATCTCGCGG ATCTTGCGGG CGTCCAACTT GCCGGTCAGT CCTTTAGGCA
            721 CCTCGTCCAC GAACACAACA CCACCGCGCA GCTTCTTGGC GGTTGTAACC TGGCTGGCCA
            781 CATAGTCCAC GATCTCCTTC TCGGTCATGG TTTTACCGTG TTCCAGCACG ACGACTGCGG
            841 CGGGCAGCTC GCCGGCATCG TCGTCGGGCA GGCCGGCGAC CCCGGCGTCG AAGATGTTGG
            901 GGTGTTGCAG CAGGATGCTC TCCAGTTCGG CTGGGGCTAC CTGGTAGCCC TTGTATTTGA
            961 TCAGGCTCTT CAGCCGGTCC ACGATGAAGA AGTGCTCGTC CTCGTCCCAG TAGCGATGTC
           1021 GCCGCTGTGC AGCCAGCCGT CCTTTGTCGA TGAGAGCGTT TGTAGCCCTC GGGTGTACGT
           1081 AGCCGCTCAT GAATCATTGG GGCCACGACG CACAGCTCGC GCGCTGATCA CAACTAGTTC
          1141 CTACCGGAAT GTTACGTCAG AGAGT
          //
          Primer: SV40pro_F
          Region: SV40 pro/ori, PuroR, poly(A) signal
          Sequence file: RDB14515_A6Lfd.seq check
          >D03972A1_A6Lf_1_SV40pro_F_C07_03_ABI08.ab1
              1 CCCCGTCTTT GCATCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
             61 GCTCGATTCT TCTGACACTA GCGCCACCAT GACCGAGTAC AAGCCTACCG TGCGCCTGGC
            121 CACTCGCGAT GATGTGCCCC GCGCCGTCCG CACTCTGGCC GCCGCTTTCG CCGACTACCC
            181 CGCTACCCGG CACACCGTGG ACCCCGACCG GCACATCGAG CGTGTGACAG AGTTGCAGGA
            241 GCTGTTCCTG ACCCGCGTCG GGCTGGACAT CGGCAAGGTG TGGGTAGCCG ACGACGGCGC
            301 GGCCGTGGCC GTGTGGACTA CCCCCGAGAG CGTTGAGGCC GGCGCCGTGT TCGCCGAGAT
            361 CGGCCCCCGA ATGGCCGAGC TGAGCGGCAG CCGCCTGGCC GCCCAGCAGC AAATGGAGGG
            421 CCTGCTTGCC CCCCATCGTC CCAAGGAGCC TGCCTGGTTT CTGGCCACTG TAGGAGTGAG
            481 CCCCGACCAC CAGGGCAAGG GCTTGGGCAG CGCCGTCGTG TTGCCCGGCG TAGAGGCCGC
            541 CGAACGCGCC GGTGTGCCCG CCTTTCTCGA AACAAGCGCA CCAAGAAACC TTCCATTCTA
            601 CGAGCGCCTG GGCTTCACCG TGACCGCCGA TGTCGAGGTG CCCGAGGGAC CTAGGACCTG
            661 GTGTATGACA CGAAAACCTG GCGCCTAATG ATCTAGAACC GGTCATGGCC GCAATAAAAT
            721 ATCTTTATTT TCATTACATC TGTGTGTTGG TTTTTTGTGT GTTCGAACTA GATGCTGTCG
            781 ACCGATGCCC TTGAGAGCCT TCAACCCAGT CAGCTCCTTC CGGTGGGCGC GGGGCATGAC
            841 TATCGTCGCC GCACTTATGA CTGTCTTCTT TATCATGCAA CTCGTAGGAC AGGTGCCGGC
            901 AGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGGCGA
            961 GCGGTATCAG CTCACTCAAA GGCGGGTAAT ACGGGTTATC CACAGAAATC ATGGGATACG
           1021 CAGCAAGGAC CATGTGGAGC CAAAATCCCA GCAAAATGTC AGGAATCGTA AAAACGGCCC
          1081 GCCGT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Fukuoka, N., Eomesodermin promotes interferon-gamma expression and binds to multiple conserved noncoding sequences across the Ifng locus in mouse thymoma cell lines. Genes Cells, 21 (2): 146-162 (2016). PMID 26749212.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl