Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-IRES-GFP_CD19-2YF

Retroviral expression vector of mouse CD19-2YF mutation in the two YxxM motifs (Y482 and Y513).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested. Additional terms and conditions: Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。 付加的提供条件： pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14512_A6Lep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' Sequence file: RDB14512_A6Lea.seq
>D04073B3_A6Le_1_pBABE5'_F01_16_ABI24.ab1     1 GTCTTAATAT GGCATATGAG ATCTTATATG GGGCACCCCC GCCCCTTGTA AACTTCCCTG    61 ACCCTGACAT GACAAGAGTT ACTAACAGCC CCTCTCTCCA AGCTCACTTA CAGGCTCTCT   121 ACTTAGTCCA GCACGAAGTC TGGAGACCTC TGGCGGCAGC CTACCAAGAA CAACTGGACC   181 GACCGGTGGT ACCTCACCCT TACCGAGTCG GCGACACAGT GTGGGTCCGC CGACACCAGA   241 CTAAGAACCT AGAACCTCGC TGGAAAGGAC CTTACACAGT CCTGCTGACC ACCCCCACCG   301 CCCTCAAAGT AGACGGCATC GCAGCTTGGA TACACGCCGC CCACGTGAAG GCTGCCGACC   361 CCGGGGGTGG ACCATCCTCT AGACTGCCGG ATCCCAGTGT GGTGGTACGG GAATTCCTGC   421 AGGCCTCGAG CACCATGCCA TCTCCTCTCC CTGTCTCCTT CCTCCTCTTT CTTACCTTAG   481 TAGGAGGCAG GCCCCAGAAG TCCTTACTGG TGGAGGTAGA AGAGGGAGGC AATGTTGTGC   541 TGCCATGCCT CCCGGACTCC TCACCTGTCT CTTCTGAGAA GCTGGCTTGG TATCGAGGTA   601 ACCAGTCAAC ACCCTTCCTG GAGCTGAGCC CCGGGTCCCC TGGCCTGGGA TTGCACGTGG   661 GGTCCCTGGG CATCTTGCTA GTGATTGTCA ATGTCTCAGA CCATATGGGG GGCTTCTACC   721 TGTGCCAGAA GAGGCCCCCT TTCAAGGACA TCTGGCAGCC TGCCTGGACA GTGAACGTGG   781 AGGATAGTGG GGGAGATGTT CCGGTGGAAT GCTTCAGACG TCAGGGACCT GGACTGTGAC   841 CTAAGGAACA GGTCCTCTGG GAGCCACAGG TCCACTTCTG GTTCCCAGCT GTATGTGTGG   901 GCTAAAGACC ATCCTAAGGT CTGGGGAACA AAGCCTGTAT GTGCCCCTCG GGGGAGCAGT   961 TTGAATCAGA GTCTAATCAA CCAAGATCTC ACTGTGGCAC CCGGGCTCCA CACTTTGGCT  1021 GTCCTGTGGG GTACCCCCTG TCCCAGTGGG CCAAAGGCTC ATCTCCTGGA CCCATGTGCC  1081 ATCTAGAGAC CTAATGTTTT CACTACTTGA GCTAGTCATG GGGGAGAGCA CCTCGTCAGA 1141 AGAAAGAATG GTTGGGCTTT TG //
Primer: IRES-R Sequence file: RDB14512_A6Leb.seq
>D04073B3_A6Le_1_IRES-R_D03_12_ABI24.ab1     1 GCATCAGGCG GCTCGGCAGT ACGTTAGGGG GGGGGGAGGG AGAGGGGGGG GGGGGGCGGA    61 ATTTACGTAG CGGCCGCTCA CGTGGTTCCC CAAGTCCCCA TGTGGCCCTC TCCTTCCCAT   121 GCTGGTTCTA GGTCGTCAGA CTTATCCATG TTTTCAAAAG AGTCTGCATC TTCTTCATGA   181 CTGGGACCGG ACTGAATTGA GTGGAGCTGA GGAGCTGCAT AGAGGATCCC TCTCATATCT   241 TCAAAGGACT GGGACCCAAG CGAGGATGCT TCCCGGCTGG GGTCCCACGC AGACCCATGG   301 GGGCTCAGGA AGTCCATCAT CCTGCCAACT GGTTGGGCCA GCTCCTCATC TGCATTTTCA   361 TAAGACTCAG CATTGGAGAA GGAGTCTTCT TCCTCTGGAC CCATGGGCTC ATCCTCGGGG   421 TTCTCATAGC CACTCCCATC CTGGGAAACC TGGTCCTGCC CAAGGTTGGA GTCGTTCTCA   481 TAGAATTCAG AGCCCTCCTC GCTGTCTGGC TCTTCATAGG CCTCCCCTTC TTCTTCCAGT   541 CCTGTTTCAT GGCTCTGAGC TCCAGTATCC TGGACTTGAA TGCGTGGATT TCCATAGGAC   601 CCAGGGACAC TCCCTAGGCC AGCAGCCCAA CGCTGAGCAT GGGCCTGGCC AGAGGTAGAT   661 GTAGGAAGGG AGAGCACATT CCCGTACTGG TTCTGGGTCC CGTTTCCCGA GGGAGGCGTC   721 ACTTTGAAGA ATCTCCTGGC GGGGTCAGTC ATTCGCTTCC TTTTCCTTCT CAGGATAAAG   781 GCTCTTTGAC AATAGAGAAA AGCCACCAGA GAAACCATAC AGAAGATGAC ATATACTAAA   841 GTCACCACTG GGACTATCCA TCCACCAGTT CTCAACAGCC AGAGCCACAC TGCTGACCTT   901 GCAATGACCT TCACGTGCCT CTCGATGGTC AGGTTTCCTC GGAGACAATA ATAGGTGCCT   961 TCATCTAAAG CTGTGGCTTG GGGGCAGAAG CAGAAGAGAC CCCCAAAACC CACATCTCTC  1021 TGACCGGGGT GCTCTCCCCC CAAGGCTTTA GGCTCAGTAG TGAAACATTA GGTCTCCTAG  1081 GGATGCACAT GGGTCCAGGG AGATGGGAGC TTGACAACTG CAACAGGGGG TACCCCACAG 1141 GACCAGCCAA AAGTGTTTGA GTG //
Primer: IRES-F Sequence file: RDB14512_A6Lec.seq
>D04073B3_A6Le_1_IRES-F_H08_23_ABI24.ab1     1 GGAAGTCTTT ATGTGTTTAG TCGAGGTTAA AAAAACGTCT AGGCCCCCCG AACCACGGGG    61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC   121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC   181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG   241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG   301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC   361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC   421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG   481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC   541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC   601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG   661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG   721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG   781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA   841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA AGGGGGGAAT   901 GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCCATTT TTGCAAGGCA   961 TGGAAAAATT ACATAAACTG AGAATAGAGA AAGTTCAGAT CAAGGTCAGA CAGATGGGAA  1021 CAGCTGATTA TGGGCCCAAA CAGGATTATC TGTGGTAGGC AGGTTTCCTG CCCCGCCTCA 1081 GTCAGATCAG ATGGAAACAG CCTGAAATAT TTGGGGCCC //
Primer: Amp_R Sequence file: RDB14512_A6Led.seq
>D04073B3_A6Le_1_Amp_R_F09_18_ABI24.ab1     1 CTCGTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT    61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC   121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT   181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA   241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC   301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC   361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG   421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT   481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC   541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA   601 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT   661 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA   721 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG   781 AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT   841 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG   901 GAACTGACGA GTTCGGAACA CCCGGGCCGC AACCCTGGGA GACGTCCCAG GGACTTCGGG   961 GGCCGTTTTT GTGGCCCGAC CTGAGTCCAA AAATCCCGAT CGTTTTGGGA CTCTTTGGTG  1021 CAACCCCCTT TAGAGAAGGA TATGTGATCC TGTTAGGAGA CGAGCACCTT AAACAGTCCG 1081 CTCCGTCTGG ATTTTTTGCT TTTCCGG //
Primer: SV40pA3'element Reverse Sequence file: RDB14512_A6Lee.seq
>D04073B3_A6Le_1_SV40pA3element Reverse_G09_21_ABI24.ab1     1 GAGTAGGGGG CGGACCTATG GAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT    61 TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT   121 ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG   181 TCAGTGAGCG AGGAAGCGGA AGAGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG   241 CCGATTCATT AATGCAACTA TGGCCATTTA ATGTAAATAC TTAAGAAAAA AAACCAAATT   301 AATTTTGATA CATGCTGCAT GTGAAAGACC CCCGCTGACG GGTAGTCAAT CACTCAGAGG   361 AGACCCTCCC AAGGAACAGC GAGACCACAA GTCGGATGCA ACTGCAAGAG GGTTTATTGG   421 ATACACGGGT ACCCGGGCGA CTCAGTCAAT CGGAGGACTG GCGCCCCGAG TGAGGGGTTG   481 TGGGCTCTTT TATTGAGCTC GGGGAGCAGA AGCGCGCGAA CAGAAGCGAG AAGCGAACTG   541 ATTGGTTAGT TCAAATAAGG CACAGGGTCA TTTCAGGTCC TTGGGGCACC CTGGAAACAT   601 CTGATGGTTC TCTAGAAACT GCTGAGGGCT GGACCGCATC TGGGGACCAT CTGTTCTTGG   661 CCCTGAGCCG GGGCAGGAAC TGCTTACCAC AGATATCCTG TTTGGCCCAT ATTCAGCTGT   721 TCCATCTGTT CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA TCCTGTTTGG   781 CCCATATTCA GCTGTTCCAT CTGTTCCTGA CCTTGATCTG AACTTCTCTA TTCTCAGTTA   841 TGTATTTTTT CCATGCCTTG CAAAATGGCG TTACTTAAGC TAGCTTGCCA AACCTACAGG   901 TGGGGGTCTT TCATTCCCCC CCTTTTTTCT GGAGACTAAA TAAAATCTTT TTATTTTTAT   961 CGTCGACCAC TGTGCTGGGC GGGCCCGGGC CGCTTTTACT TTGTACAGCT CGTCCCATGC  1021 CCGAGAGGTG ATCCTGGCGC GGGTCACGAA CTCAAGCCAA GACCCATGTG ATTCGCGCAT 1081 CTCGATGGGG GATCTTTGCC TCCAGGGGCC CGGTAC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl