Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMXs-IRES-mCherry_IgG1

Retroviral expression vector of mouse Immunoglobulin G1 (IgG1).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
Additional terms and conditions for distribution	Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 （Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016） を引用し、寄託者への謝辞の表明を必要とする。
付加的提供条件	pMXsベクターについて：利用者は、研究成果の公表にあたって pMXsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄先生への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14509_A6Lep1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' Sequence file: RDB14509_A6Lea.seq
>D04073A9_A6Le_1_pBABE5'_E01_13_ABI24.ab1     1 GTTTTATAAG GGCCATATGA GATCTTATAT GGGGCACCCC CGCCCCTTGT AAACTTCCCT    61 GACCCTGACA TGACAAGAGT TACTAACAGC CCCTCTCTCC AAGCTCACTT ACAGGCTCTC   121 TACTTAGTCC AGCACGAAGT CTGGAGACCT CTGGCGGCAG CCTACCAAGA ACAACTGGAC   181 CGACCGGTGG TACCTCACCC TTACCGAGTC GGCGACACAG TGTGGGTCCG CCGACACCAG   241 ACTAAGAACC TAGAACCTCG CTGGAAAGGA CCTTACACAG TCCTGCTGAC CACCCCCACC   301 GCCCTCAAAG TAGACGGCAT CGCAGCTTGG ATACACGCCG CCCACGTGAA GGCTGCCGAC   361 CCCGGGGGTG GACCATCCTC TAGACTGCCG GATCCCAGTG TGGTGGTACG GGAATTCACA   421 CAGGACCTCA CCATGGGATG GAGCTGTATC ATGCTCTTCT TGGCAGCAAC AGCTACAGGT   481 GTCCACTCCC AGGTCCAACT GCAGCAGCCT GGGGCTGAGC TTGTGAAGCC TGGGGCTTCA   541 GTGAAGCTGT CCTGCAAGGC TTCTGGCTAC ACCTTCACCA GCTACTTGAT GCACTGGGTG   601 AAGCAGAGGC CTGGACGAGG CCTTGAGTGG ATTGGAAGGA TTGATCCTAA TAGTGGTGGT   661 ACTAAGTACA ATGAGAAGTT CAAGAGCAAG GCCACACTGA CTGTAGACAA ACCCTCCAGC   721 ACAGCCTACA TGCAGCTCAG CAGCCTGACA TCTGAGGACT CTGCGGTCTA TTATTGTGCA   781 AGATATGATT ACTACGGTAG TAGCTACTTT GACTACTGGG GCCAAGGCAC CACTCTCACA   841 GTCTCGAGTG CCAAAACGAC ACCCCCATCT GTCTATCCAC TGGCCCCTGG ATCTGCTGCC   901 CAAACTAACT CCATGGTGAC CCTGGGATGC CTGGTCAAGG GCTATTTCCC TGAGCCAGTG   961 ACAGTGACCT GGAACTCTGG ATCCCTGTCC AGCGGTGTGC ACACCCTTCC CAGCTGTCCT  1021 GCAGTCTGAC CTCTACACTC TGAGCAGCTC AGTGACTGTC CCCTCCAGCA CCTGGCCAAG  1081 CAGGACGTCA CTGCACGTTG CCCAACCCGT CAGCAGCACA GTTGACAGAA TGTGCCAGGC 1141 ATGTGATGTA AGCCTATTGT GGCA //
Primer: IRES-R Sequence file: RDB14509_A6Leb.seq
>D04073A9_A6Le_1_IRES-R_A03_03_ABI24.ab1     1 GCATCAGCGG GGCTTCGGCA GTAACGTTAG GGGGGGGGGA GGGAGAGGGG GGGGGGGGGC    61 GGAATTTACG TAGCGGCCGC CTAGGGCGCT TGCCCAATCA TGTTCTTGTA TTCAGGAACC   121 AGTGTCTGCT TCAGCTCCAC CACCGAGGAG AAGATCCACT TTACCTTGAA GAGTGTGACA   181 GCAGCGCTGT AGCACACACT GAGCAGGAAG AGGCTGATGA AGATGGTGAT GGTCGTCCAG   241 AGCCCGTCCA GCTCCCCGTC CTGGGCCTCA GCACAGGTCT CGTCCAGTTG CAGCCCAGGA   301 GAGTGGGAGA GGCTCTTCTC AGTATGGTGG TTGTGCAGGC CCTCATGTAA CACAGAGCAG   361 GTGAAAGTAT TTCCTGCCTC CCAGTTGCTC TTCTGCACAT TGAGCTTGCT GTAGACGAAG   421 TAAGAGCCAT CTGTGTCCAT GATGGGCTGA GTGTTCTTGT AGTTCTCCGC TGGCTGCCCA   481 TTCCACTGCC ACTCCACAGT AATGTCTTCA GGGAAGAAGT TTGTTATCAT GCAGGTCAGA   541 CTGACTTTAT CCTTGGCCAT CTGCTCCTTG GGAGGTGGAA TGGTGTACAC CTGTGGAGCC   601 TTCGGTCTGC CTTTGGTTTT GGAGATGGTT TTCTCGATGG GGGCAGGGAA AGCTGCACTG   661 TTGACCCTGC ATTTGAACTC CTTGCCATTG AGCCAGTCCT GGTGCATGAT GGGAAGTTCA   721 CTGACTGAAC GGAAAGTGCT GTTGATCTGC TCCTCCCGGG GTTTCGTCTG AGCTGTGTGC   781 ACCTCCACAT CATCTACAAA CCAGCTGAAC TGGACCTCGG GATCATCCTT GCTGATGTCT   841 ACCACAACAC ACGTGACCTT AGGAGTCAGA GTAATGGTGA GCACATCCTT GGGCTTTGGG   901 GGGAAGATGA AGACAGATGA TACTTCTGGG ACTGTACATA TGCAAGGCTT ACAACCACAA   961 TCCCTGGGCA CAATTTTCTT GTCCACCTTT GGTGCTGCTG GCCGGGTGGG CAACGTTGCA  1021 GTGACGGTCT GGCTGGGCCA GGTGCTGGAG GGGACAGTCA CTGAGCTGCT CAGAGTGTAG  1081 AGTCAGACTG CAAGACAGCT GGCAGGTGTG CAACCGCTGG ACAGATTCCA GAGTTCAAGT 1141 CACTGTCACT GGCTTCAAGG //
Primer: IRES-F Sequence file: RDB14509_A6Lec.seq
>D04073A9_A6Le_1_IRES-F_C12_09_ABI24.ab1     1 TGCATCATTC TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC    61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGGTGA GCAAGGGCGA   121 GGAGGATAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC   181 CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC   241 CCAGACCGCC AAGCTGAAGG TGACCAAGGG TGGCCCCCTG CCCTTCGCCT GGGACATCCT   301 GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA   361 CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA   421 CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA   481 GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT   541 GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT   601 CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCTGAGGTCA AGACCACCTA   661 CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA AGTTGGACAT   721 CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAACGCGCCG AGGGCCGCCA   781 CTCCACTCTC GGCATGGACG AGCTGTACAA GTAAGCGGCC CGATAAAATA AAAGATTTTA   841 TTTAGTCTCC AGAAAAAGGG GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT   901 TAAGTAACGC CATTTTGCAA GGCATGGAAA AATACATAAC TGAGAATAGA GAAGTTTCAG   961 ATCAAGGTCA GGAACAGATG GAAACAGCTG AATATGGGCC AAACAGATAT CTGTGGTAAG  1021 CAGTTCCCTG CCCCGGGCTC AGGTCAGACA GATGGGACAG CTGATATGGG CCCAACAGGA 1081 TATCTGTGTT AGGCAGTCTG GCCTCCGGAC CTCCAGGTCC TAGA //
Primer: Amp_R Sequence file: RDB14509_A6Led.seq
>D04073A9_A6Le_1_Amp_R_D12_12_ABI24.ab1     1 CTTCGTTTTC ATATTATTGA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT    61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC   121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT   181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA   241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC   301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC   361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG   421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT   481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC   541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA   601 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT   661 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA   721 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG   781 AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT   841 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG   901 GAACTGACGA GTTCGGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCACG GACTTCGGGG   961 GCCCGTTTTT TGTGGCCCGA CCTGAGTCCC AAAAATCCGA TCGTTTTTGG ACTCTTTGGG  1021 TGCACCCCCC TTTAGAGGAG GGATATGTTG GATTCTGGGT AAGGAGACGA GACCTAAACC 1081 AGTTCCCCGC CTCGGTCTGA ATTTTTTGGC AATTCG //
Primer: SV40pA3'element Reverse Sequence file: RDB14509_A6Lee.seq
>D04073A9_A6Le_1_SV40pA3element Reverse_E12_15_ABI24.ab1     1 GTTGTCGGGG GGGCGGAGCC TATGGAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG    61 CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA   121 ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA   181 GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCCAATACG CAAACCGCCT CTCCCCGCGC   241 GTTGGCCGAT TCATTAATGC AACTATGGCC ATTTAATGTA AATACTTAAG AAAAAAAACC   301 AAATTAATTT TGATACATGC TGCATGTGAA AGACCCCCGC TGACGGGTAG TCAATCACTC   361 AGAGGAGACC CTCCCAAGGA ACAGCGAGAC CACAAGTCGG ATGCAACTGC AAGAGGGTTT   421 ATTGGATACA CGGGTACCCG GGCGACTCAG TCAATCGGAG GACTGGCGCC CCGAGTGAGG   481 GGTTGTGGGC TCTTTTATTG AGCTCGGGGA GCAGAAGCGC GCGAACAGAA GCGAGAAGCG   541 AACTGATTGG TTAGTTCAAA TAAGGCACAG GGTCATTTCA GGTCCTTGGG GCACCCTGGA   601 AACATCTGAT GGTTCTCTAG AAACTGCTGA GGGCTGGACC GCATCTGGGG ACCATCTGTT   661 CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA TCCTGTTTGG CCCATATTCA   721 GCTGTTCCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC TGCTTACCAC AGATATCCTG   781 TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CCTGACCTTG ATCTGAACTT CTCTATTCTC   841 AGTTATGTAT TTTTTCCATG CCTTGCAAAA TGGCGTTACT TAAGCTAGCT TGCCAAACCT   901 ACAGGTGGGG TCTTTCATTC CCCCCCTTTT TTCTGGAGAC TAAATAAAAT CTTTTTATTT   961 TTATCGGGGC CGCTTTACTT GTAACAGCTT CGTTCCATGC CCGAGAGTGG AGTGGGCGGC  1021 CCCTTCGGCG CGTTTCGTAC TGTTCCCACG GATGGTGTAG TCCCTCGATT GTGGGAGTGA 1081 ATGTTCTAAA CTTGAATGAT TGAACGGTTT GATAAGGGCT GTCCG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl