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pEF/HRE-Fluc

HIF-1 dependent promoter reporter containing HRE.

Catalog number RDB14404
Resource name pEF/HRE-Fluc
Alternative name HRE-Fluc
Clone info. HIF-1 dependent promoter reporter containing HRE of human VEGF sequence and firefly luciferase (HRE-Fluc).
Comment Promoter region of pEF-1 and myc-tag were replaced with HRE-Fluc fragment. Expression was confirmed by the depositor with luciferase assay.
Vector backbone pEF6/Myc-His B plasmid (plasmid)
Size of vector backbone 5.8 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human VEGF genomic DNA firefly (Photinus pyralis) luciferase cDNA
Depositor|Developer Kizaka-Kondoh, Shinae |
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External Database
human VEGF firefly (Photinus pyralis) luciferase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Hoang, NT. et al. Cancer. Sci., 107 (8): 1151-1158, 2016) RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Hoang, NT. et al. Cancer. Sci., 107 (8): 1151-1158, 2016)。本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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          RDB14404 pEF/HRE-Fluc DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hoang, N.T., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HIF-1 signaling pathway (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14404_A6Kpp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Amp_R
          Sequence file: RDB14404_A6Kpa.seq check
          >14404_14404_A6Kp_2_Amp_R_E02_05_ABI08.ab1
              1 CTCCCGTTTT AATATTATTG AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
             61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
            121 CACCTGACGT CAGATCGACG GATCGGGAGA TCGGTACCGA GCTTTTCTCG AGCCACAGTG
            181 CATACGTGGG CTCCAACAGG TCCTCTTGTC GAGCCACAGT GCATACGTGG GCTCCAACAG
            241 GTCCTCTTGT CGAGCCACAG TGCATACGTG GGCTCCAACA GGTCCTCTTG TCGAGCCACA
            301 GTGCATACGT GGGCTCCAAC AGGTCCTCTT GTCGAGCCAC AGTGCATACG TGGGCTCCAA
            361 CAGGTCCTCT TGTCGAGATC TGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGAGCT
            421 CGTTTAGTGA ACCGTCAGAT CACTAGAAGC TTGGCATTCC GGTACTGTTG GTAAAGCCAC
            481 CATGGAAGAC GCCAAAAACA TAAAGAAAGG CCCGGCGCCA TTCTATCCGC TGGAAGATGG
            541 AACCGCTGGA GAGCAACTGC ATAAGGCTAT GAAGAGATAC GCCCTGGTTC CTGGAACAAT
            601 TGCTTTTACA GATGCACATA TCGAGGTGGA CATCACTTAC GCTGAGTACT TCGAAATGTC
            661 CGTTCGGTTG GCAGAAGCTA TGAAACGATA TGGGCTGAAT ACAAATCACA GAATCGTCGT
            721 ATGCAGTGAA AACTCTCTTC AATTCTTTAT GCCGGTGTTG GGCGCGTTAT TTATCGGAGT
            781 TGCAGTTGCG CCCGCGAACG ACATTTATAA TGAACGTGAA TTGCTCAACA GTATGGGCAT
            841 TTCGCAGCCT ACCGTGGTGT TCGTTTCCAA AAAGGGGTTG CAAAAAATTT TGAACGTGCA
            901 AAAAAAGCTC CCAATCATCC AAAAAATTAT TATCATGGAT TCTAAAACGG ATTACCAGGG
            961 ATTTCAGTCG ATGTACACGT TCGTCACATC TCATCTACCT CCCGGGTTTT ATGATACGAT
           1021 TTTGTGCCAG AGGTCCTTCG ATAGGACAGA ACAATGCACT GATCATGAAC TTCTCTGGAT
           1081 CTACTGGATC TGCCTAGTGT CGCCTCTGGC TCATAGAACC TGGCCTTGGC CGTGAAGAAT
          1141 TTCTT
          //
          Primer: BGH_rev2
          Sequence file: RDB14404_A6Kpb.seq check
          >RDB14404_14404_A6Kp_2_BGH_rev2_E12_15_ABI24.ab1
              1 CTTTCAGGGT CAGGAGCACG GGGGAGGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC
             61 AGTCGAGGCT GATCAGCGAG CTTCTAGAAT TACACGGCGA TCTTTCCGCC CTTCTTGGCC
            121 TTTATGAGGA TCTCTCTGAT TTTTCTTGCG TCGAGTTTTC CGGTAAGACC TTTCGGTACT
            181 TCGTCCACAA ACACAACTCC TCCGCGCAAC TTTTTCGCGG TTGTTACTTG ACTGGCGACG
            241 TAATCCACGA TCTCTTTTTC CGTCATCGTC TTTCCGTGCT CCAAAACAAC AACGGCGGCG
            301 GGAAGTTCAC CGGCGTCATC GTCGGGAAGA CCTGCGACAC CTGCGTCGAA GATGTTGGGG
            361 TGTTGGAGCA AGATGGATTC CAATTCAGCG GGAGCCACCT GATAGCCTTT GTACTTAATC
            421 AGAGACTTCA GGCGGTCAAC GATGAAGAAG TGTTCGTCTT CGTCCCAGTA AGCTATGTCT
            481 CCAGAATGTA GCCATCCATC CTTGTCAATC AAGGCGTTGG TCGCTTCCGG ATTGTTTACA
            541 TAACCGGACA TAATCATAGG ACCTCTCACA CACAGTTCGC CTCTTTGATT AACGCCCAGC
            601 GTTTTCCCGG TATCCAGATC CACAACCTTC GCTTCAAAAA ATGGAACAAC TTTACCGACC
            661 GCGCCCGGTT TATCATCCCC CTCGGGTGTA ATCAGAATAG CTGATGTAGT CTCAGTGAGC
            721 CCATATCCTT GCCTGATACC TGGCAGATGG AACCTCTTGG CAACCGCTTC CCCGACTTCC
            781 TTAGAGAGGG GAGCGCCACC AGAAGCAATT TCGTGTAAAT TAGATAAATC GTATTTGTCA
            841 ATCAGAGTGC TTTTGGCGAA GAAGGAGAAT AGGGTTGGCA CCAGCAGCGC ACTTTGAATC
            901 TTGTAATCCT GAAAGCTCCT CAGAAACAGC TCTTCTTCAA TCTATACATT AAGACGACTC
            961 GAAATCCACA TATCAATATC CGAGTGTAGT AAACATTCCA AAACCGTGAT GGAATGGAAC
           1021 AACACTTTAA AATCGCAGTA TCCGGAATGA TTTGATTTGC CCAAAATAGA TCTCTGGGCA
           1081 TGCGAGAATC TCACGCAGGC AGTTCTATTG AGCAGAGGCG ACAACCCTTT TTAAGGCCAG
          1141 GAAC
          //
          Primer: SV40pro_F
          Sequence file: RDB14404_A6Kpc.seq check
          >RDB14404_14404_A6Kp_2_SV40pro_F_F12_18_ABI24.ab1
              1 CGCTTGGTCC GTACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
             61 AAAGCTCCCC CCCCGGGAGG TCCACAATGG TTGAACAAGA TGGATTGCAC GCAGGTTCTC
            121 CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT
            181 CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG
            241 ACCTGTCCGG TGCCCTGAAT GAACTCCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA
            301 CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC
            361 TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA
            421 AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC
            481 CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC
            541 TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG
            601 CCAGGCTCAA GGCGCGTATG CCCGACGGCG AGGATCTCGT CGTGACTCAT GGCGATGCCT
            661 GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC
            721 TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC
            781 TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC
            841 AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGGTTCG
            901 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCC
            961 TTCTATGAAA AGGTTGGGGC TTCGGAAATC GTTTTCCCGG GACGCCGGGC TGGGATGATC
           1021 CTCCAGCGCG GGGATCTCAT GCTGGGAGTT CTTTCGCCAC CCCACTTTGT TTATTTGCAG
           1081 CCTTATAATG TACAAATAAA GCAATAGCCA TCACAAGTTT CACAAATAGC CATTTTTTTC
          1141 AGTGCATCTA GTTGTGATTG TTCCCGAC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hoang, N.T., Hypoxia-inducible factor-targeting prodrug TOP3 combined with gemcitabine or TS-1 improves pancreatic cancer survival in an orthotopic model. Cancer Sci. 107 (8): 1151-1158 (2016). PMID 27270607.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl