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pBabe-hSTING(V155M_C88S_C91S)

Retroviral expression vector of human STING, V155M_C88S_C91S mutant.

Catalog number RDB14358
Resource name pBabe-hSTING(V155M_C88S_C91S)
Clone info. Mammalian retroviral vector for expressing human STING (mutation, V155M. dual mutation, C88S/C91S). Suppression of STING palmitoylation inhibits the gain-of-function phenotype in the SAVI–STING.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pBabe-Puro (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name human TMEM173 cDNA
Depositor|Developer Arai, Hiroyuki | Taguchi, Tomohiko |
Other clones in our bank

External Database
human TMEM173

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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          RDB14358 pBabe-hSTING(V155M_C88S_C91S) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14358_A6J7p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBABE5'
          Sequence file: RDB14358_A6J7a.seq check
          >D03866A8_A6J7_1_pBABE5'_H01_22_ABI24.ab1
              1 AGGGGTGCGG GGATCAGGTG TGGTGGTACG TAGGAATTCG CCGCCACCAT GCCCCACTCC
             61 AGCCTGCATC CATCCATCCC GTGTCCCAGG GGTCACGGGG CCCAGAAGGC AGCCTTGGTT
            121 CTGCTGAGTG CCTGCCTGGT GACCCTTTGG GGGCTAGGAG AGCCACCAGA GCACACTCTC
            181 CGGTACCTGG TGCTCCACCT AGCCTCCCTG CAGCTGGGAC TGCTGTTAAA CGGGGTCTGC
            241 AGCCTGGCTG AGGAGCTGCG CCACATCCAC TCCAGGTACC GGGGCAGCTA CTGGAGGACT
            301 GTGCGGGCCA GCCTGGGCAG CCCCCTCCGC CGTGGGGCCC TGTTGCTGCT GTCCATCTAT
            361 TTCTACTACT CCCTCCCAAA TGCGGTCGGC CCGCCCTTCA CTTGGATGCT TGCCCTCCTG
            421 GGCCTCTCGC AGGCACTGAA CATCCTCCTG GGCCTCAAGG GCCTGGCCCC AGCTGAGATC
            481 TCTGCAGTGT GTGAAAAAGG GAATTTCAAC ATGGCCCATG GGCTGGCATG GTCATATTAC
            541 ATCGGATATC TGCGGCTGAT CCTGCCAGAG CTCCAGGCCC GGATTCGAAC TTACAATCAG
            601 CATTACAACA ACCTGCTACG GGGTGCAGTG AGCCAGCGGC TGTATATTCT CCTCCCATTG
            661 GACTGTGGGG TGCCTGATAA CCTGAGTATG GCTGACCCCA AACATTCGCT TCCTGGATAA
            721 AACTGCCCCA GCAGACCGGT GACCATGCTG GCATCAAAGG ATCGGGTTTA CAGCAACAGC
            781 ATCTATGAGC TTTCTGGGAG AAACGGGCAG CGGGCGGGGC ACCTGTGTCC TGGAGTACGC
            841 CACCCCCTTG CAGACTTTGT TTGCCATGTC ACAATACAGT CAAGCTTGGC TTTAGCCGGG
            901 AAGAATAGGC TTTGAGCAGG CCCAAACTCT TTCTGCCCGG ACACTTGAAG GAACATCCTG
            961 GCAATTGCCC CCTGAAGTCT CAGAACAAAC TGCCGCCCTC ATTGCCTAAC CAGGGAACCC
           1021 TGCAGATGAC AGCAGCTTTC CTCGCCTGAT CCCCAGAAGG TTCTCCGGCA CCTGGCGGCC
          1081 ATGAAAGAAA AGGAAAGAAA GGTTAT
          //
          Primer: PuroR-rev
          Sequence file: RDB14358_A6J7b.seq check
          >D03866A8_A6J7_1_PuroR-rev_H06_08_ABI08.ab1
              1 GATAGGCTTT TTGCAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT
             61 CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG
            121 GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA
            181 TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG
            241 ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG
            301 GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGGGTC GAGTCAAGAG
            361 AAATCCGTGC GGAGAGGGAG GGGCTTTTCC ATTCCACTGA TGAGGAGCTC AGGCTCTTGG
            421 GACATCGTGG AGGTACTGGG CACCGCTGAG GTCTTCAAGC TGCCCACAGT AACCTCTTCC
            481 TTTTCCTCCT GCCGCAGGTG CCGGAGAACC TCCTGGGACA GCGAGAAGCT GCTGTCATCT
            541 GCAGGTTCCT GGTAGGCAAT GAGGCGGCAG TTGTTCTGAG ACTCAGGGGC ATCTGCCAGG
            601 ATGTCCTCAA GTGTCCGGCA GAAGAGTTTG GCCTGCTCAA GCCTATCCTC CCGGCTAAAG
            661 CCAGCTTGAC TGTATTGTGA CATGGCAAAC AAAGTCTGCA AGGGGGTGGC GTACTCCAGG
            721 ACACAGGTGC CCGCCCGCTG CCCGTTCTCC AGAAGCTCAT AGATGCTGTT GCTGTAAACC
            781 CGATCCTTGA TGCCAGCATG GTCACCGGTC TGCTGGGGCA GTTTATCCAG GAAGCGAATG
            841 TTGGGGTCAG CCATACTCAG GTTATCAGGC ACCCCACAGT CCAATGGGAG GAGAATATAC
            901 AGCCGCTGGC TCACTGCACC CCGTAGCAGG TTGTTGTAAT GCTGATTGTA AGTTCGAATC
            961 CGGGCCTGGA GCTCTGGCAG GATCAGCCGC AGATATCCGA TGTAATATGA CCATGCCAGC
           1021 CCCATGGGCC ATGTTGAAAT TTCCCTTTTT CACACACTGC AGAGGATCTC AGCTGGGGGC
           1081 CAGCCATGAG TCCAGGAGAT GATCAGTGCC TGCAGAGCCA AGAGGCAGGC CATCCAAGTG
          1141 AAGGGTCCGG C
          //
          Primer: Amp_R
          Sequence file: RDB14358_A6J7c.seq check
          >D03866A8_A6J7_1_Amp_R_H03_24_ABI24.ab1
              1 CTCTTTTCAA TATTATTGAA GGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
             61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
            121 CACCTGCAGC CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
            181 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
            241 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
            301 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
            361 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
            421 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGGGCG CCAGTCCTCC GATTGACTGA
            481 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT
            541 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA
            601 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG
            661 AGGCAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT
            721 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG
            781 GAACTGACGA GTTCTGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCAGGG ACTTTGGGGG
            841 CCGTTTTTGT GGCCCGACCT GAGGAAAGGG AGTCGATGTG GAATCCGACC CCGTCAGATA
            901 TGTGGGTTCT GGTAGGAGAC GAGAACCTAA AACAGTTCCC GCCCTCCGTC TGAATTTTTG
            961 CTTTCGGTTT GGAAACCGAA GCCCGCGCGT CTTGTCTGCT GCAGCGCTGC AGCATCGTCT
           1021 GTGTGTCTCT GTCTGACTGT GTTCTGTATT GTCTGAAATA GGCCCAGACT GGTAACCACT
          1081 CCCTAGTTGA ACCTTGAGTC CACTGGAAGG ATGTGTTCGA AGGCCGGG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB14358_A6J7d.seq check
          >D03866A8_A6J7_1_SV40pro_F_V2_F07_06_ABI08.ab1
              1 GTGCACTTTT TTGGAGGCTA GGCTTTTGCA AAAGCTTACC ATGACCGAGT ACAAGCCCAC
             61 GGTGCGCCTC GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG CCGCCGCGTT
            121 CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCGGAC CGCCACATCG AGCGGGTCAC
            181 CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG TGTGGGTCGC
            241 GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG CGGGGGCGGT
            301 GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG CCGCGCAGCA
            361 ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT TCCTGGCCAC
            421 CGTCGGCGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG TGCTCCCCGG
            481 AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG CGCCCCGCAA
            541 CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG TGCCCGAAGG
            601 ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA CGCCCGCCCC ACGACCCGCA
            661 GCGCCCGACC GAAAGGAGCG CACGACCCCA TGCATCGATA AAATAAAAGA TTTTATTTAG
            721 TCTCCAGAAA AAGGGGGGAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT
            781 AACGCCATTT TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAGAAGT TCAGATCAAG
            841 GTCAGGAACA GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC
            901 CTGCCCCGGC TCAGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT
            961 GTGGTAAGCA GTTCCTGCCC CGGGCTCAGG CCAAGAACAG ATGGGTCCCC AGATGCGGTC
           1021 CAGCCTCAGC AGTTTCTAGG AGACATCAGA TGTTTCCAAG GGTGCCCCAG AACTGAAATG
          1081 ACTGTGCTAA TTGAACTAAG CATCAGTTCG CATTCTTCCG CCT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl