Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBabe-hSTING(N154S_C88S_C91S)

Retroviral expression vector of human STING, N154S_C88S_C91S mutant.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14357_A6J7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' Sequence file: RDB14357_A6J7a.seq
>D03866A7_A6J7_1_pBABE5'_G01_19_ABI24.ab1     1 TGTGGCCGGA ATCCAGGTGG TGGGTGGTAC GTAGGATTCG CCGCCACCAT GCCCCACTCC    61 AGCCTGCATC CATCCATCCC GTGTCCCAGG GGTCACGGGG CCCAGAAGGC AGCCTTGGTT   121 CTGCTGAGTG CCTGCCTGGT GACCCTTTGG GGGCTAGGAG AGCCACCAGA GCACACTCTC   181 CGGTACCTGG TGCTCCACCT AGCCTCCCTG CAGCTGGGAC TGCTGTTAAA CGGGGTCTGC   241 AGCCTGGCTG AGGAGCTGCG CCACATCCAC TCCAGGTACC GGGGCAGCTA CTGGAGGACT   301 GTGCGGGCCA GCCTGGGCAG CCCCCTCCGC CGTGGGGCCC TGTTGCTGCT GTCCATCTAT   361 TTCTACTACT CCCTCCCAAA TGCGGTCGGC CCGCCCTTCA CTTGGATGCT TGCCCTCCTG   421 GGCCTCTCGC AGGCACTGAA CATCCTCCTG GGCCTCAAGG GCCTGGCCCC AGCTGAGATC   481 TCTGCAGTGT GTGAAAAAGG GAATTTCAGC GTGGCCCATG GGCTGGCATG GTCATATTAC   541 ATCGGATATC TGCGGCTGAT CCTGCCAGAG CTCCAGGCCC GGATTCGAAC TTACAATCAG   601 CATTACAACA ACCTGCTACG GGGTGCAGTG AGCCAGCGGC TGTATATTCT CCTCCCATTG   661 GACTGTGGGG TGCCTGATAA CCTGAGTATG GCTGACCCCA ACATTCGCTT CCTGGATAAA   721 CTGCCCCAGC AGACCGGTGA CCATGCTGGC ATCAAGGATC GGGTTTACAG CAACAGCATC   781 TATGAGCTTC TGGAGAACGG GCAGCGGGCG GGCACCTGTG TCCTGGAGTA CGCCACCCCC   841 TTGCAGACTT TGTTTGCCAT GTCACAAATA CAGTCAAGCT GGCTTTAGCC GGGGAGGATA   901 GGCTTGAGCA GGCCAAACTC TTCTGCCCGG ACACTTTGAG GACATCCCTG GCAGATGCCC   961 CCTGAAGTCT CAGAACAACT GCCGCCTCAT TTGCCTACCA GGAAACCTTG CAGATGACAG  1021 CAGCTTCTTC GCTGTCCAAG GAGGATCTCC GGCACCCTTG CCGGCAGGAG AAAAAGGAAG  1081 AAGGTTAACT GGTGGGCCAG CCTGGAGGAC CTTCAGCGGG TGGCCGCAAG TATTCCTCTC 1141 CTACCGAATG //
Primer: PuroR-rev Sequence file: RDB14357_A6J7b.seq
>D03866A7_A6J7_1_PuroR-rev_G06_07_ABI08.ab1     1 GTTGGGCTTT TTGCAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC    61 TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC   121 GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT   181 ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG   241 GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT   301 GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGGGT CGAGTCAAGA   361 GAAATCCGTG CGGAGAGGGA GGGGCTTTTC CATTCCACTG ATGAGGAGCT CAGGCTCTTG   421 GGACATCGTG GAGGTACTGG GCACCGCTGA GGTCTTCAAG CTGCCCACAG TAACCTCTTC   481 CTTTTCCTCC TGCCGCAGGT GCCGGAGAAC CTCCTGGGAC AGCGAGAAGC TGCTGTCATC   541 TGCAGGTTCC TGGTAGGCAA TGAGGCGGCA GTTGTTCTGA GACTCAGGGG CATCTGCCAG   601 GATGTCCTCA AGTGTCCGGC AGAAGAGTTT GGCCTGCTCA AGCCTATCCT CCCGGCTAAA   661 GCCAGCTTGA CTGTATTGTG ACATGGCAAA CAAAGTCTGC AAGGGGGTGG CGTACTCCAG   721 GACACAGGTG CCCGCCCGCT GCCCGTTCTC CAGAAGCTCA TAGATGCTGT TGCTGTAAAC   781 CCGATCCTTG ATGCCAGCAT GGTCACCGGT CTGCTGGGGC AGTTTATCCA GGAAGCGAAT   841 GTTGGGGTCA GCCATACTCA GGTTATCAGG CACCCCACAG TCCAATGGGA GGAGAATATA   901 CAGCCGCTGG CTCACTGCAC CCCGTAGCAG TTGTTGTAAT GCTGATTGTA AGTTCGAATC   961 CCGGGCCTGG AGCTCTGGCA GGATCAGCCG CAGATATCCG ATGTAATATG ACCATGCCCA  1021 GCCCATGGGC CACGCTGAAA TTTCCCTTTT TTCACACACT GCAGAGATCT CAGCTGGGGC  1081 AGTCTTGGAG CCCAGAGATG TCATGACTGC TAGAGGCCAG GAGGCAAGCC ATTCTAGTGA 1141 AGGGCCGGGG C //
Primer: Amp_R Sequence file: RDB14357_A6J7c.seq
>D03866A7_A6J7_1_Amp_R_G03_21_ABI24.ab1     1 ACTTTTTTCA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT    61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC   121 ACCTGCAGCC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT   181 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA   241 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC   301 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG   361 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC   421 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGGGCGC CAGTCCTCCG ATTGACTGAG   481 TCGCCCGGGT ACCCGTGTAT CCAATAAACC CTCTTGCAGT TGCATCCGAC TTGTGGTCTC   541 GCTGTTCCTT GGGAGGGTCT CCTCTGAGTG ATTGACTACC CGTCAGCGGG GGTCTTTCAT   601 TTGGGGGCTC GTCCGGGATC GGGAGACCCC TGCCCAGGGA CCACCGACCC ACCACCGGGA   661 GGCAAGCTGG CCAGCAACTT ATCTGTGTCT GTCCGATTGT CTAGTGTCTA TGACTGATTT   721 TATGCGCCTG CGTCGGTACT AGTTAGCTAA CTAGCTCTGT ATCTGGCGGA CCCGTGGTGG   781 AACTGACGAG TTCTGAACAC CCGGCCGCAA CCCTGGGAGA CGTCCCAGGG ACTTTGGGGG   841 CCGTTTTTGT GGCCCGACCT GAGGAAGGGA GTCGATGTGG GAATCCGACC CCGTCAGGAT   901 ATGTGGGTTC TGGTAGGAGA CGAGAACCTA AAACAGTTCC CGCCCTCCGT CTGAATTTTT   961 TGCTTTCGGT TTGGAACCGA AGCCGGCGCG TCTTGTCTGC TGCAGCGCTG CAGCATCGTT  1021 CTGTGTTGTC TCTGTTCTGA CTGTGTTTCT GTATTGTCTG AATTAGGCCC AGACTGGTAA 1081 CCCACCTCCT TAGTTGAACT TAGTTCACCT GAAAGATGGT TTCGAGAGCC GGGAAT //
Primer: SV40pro_F_V2 Sequence file: RDB14357_A6J7d.seq
>D03866A7_A6J7_1_SV40pro_F_V2_G05_20_ABI24.ab1     1 GTGCGGCCTT TTTGGGAGGC CTAGGCTTTT GCAAAAGCTT ACCATGACCG AGTACAAGCC    61 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC   121 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT   181 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT   241 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC   301 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA   361 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC   421 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC   481 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG   541 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA   601 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC   661 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATAAAATAAA AGATTTTATT   721 TAGTCTCCAG AAAAAGGGGG GAATGAAAGA CCCCACCTGT AGGTTTGGCA AGCTAGCTTA   781 AGTAACGCCA TTTTGCAAGG CATGGAAAAA TACATAACTG AGAATAGAGA AGTTCAGATC   841 AAAGGTCAGG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGGTAAGC   901 AGTTCCTGCC CCGGGCTCAG GGCCCAAGAA CAGATGGGAA CAGCTGAAAT ATGGGGCCAA   961 AACAAGGATA TCTGTGGGTA AGCAGTTTCC TGCCCTCGGC TTCAAGGGCC AAGAAACAGA  1021 TGGGTCCCCC AGAATGCGGT CCAAGCCCCT CAGCAGCTTT CTAGAGGATC AATCCAGATG 1081 TTTCCAGGGG TGCCCTAGGA ACTTGAATGA ACCCTGGTGC CTTAATTTGA AAACT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl