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pBabe-hSTING(V147L_C88S_C91S)

Retroviral expression vector of human STING, V147L_C88S_C91S mutant.

Catalog number RDB14356
Resource name pBabe-hSTING(V147L_C88S_C91S)
Clone info. Mammalian retroviral vector for expressing human STING (mutation, V147L. dual mutation, C88S/C91S). Suppression of STING palmitoylation inhibits the gain-of-function phenotype in the SAVI–STING.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pBabe-Puro (Plasmid)
Size of vector backbone 5.2 kb
Selectable markers Amp^r (E. coli), Puro^r (mammalian cells).
Gene/insert name human TMEM173 cDNA
Depositor|Developer Arai, Hiroyuki | Taguchi, Tomohiko |
Other clones in our bank

External Database
human TMEM173

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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          RDB14356 pBabe-hSTING(V147L_C88S_C91S) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14356_A6J7p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBABE5'
          Sequence file: RDB14356_A6J7a.seq check
          >D03866A6_A6J7_1_pBABE5'_F01_16_ABI24.ab1
              1 GGGGGTTGGG AACCAGTGTG GTGGTACGTA GGATTCGCCG CCACCATGCC CCACTCCAGC
             61 CTGCATCCAT CCATCCCGTG TCCCAGGGGT CACGGGGCCC AGAAGGCAGC CTTGGTTCTG
            121 CTGAGTGCCT GCCTGGTGAC CCTTTGGGGG CTAGGAGAGC CACCAGAGCA CACTCTCCGG
            181 TACCTGGTGC TCCACCTAGC CTCCCTGCAG CTGGGACTGC TGTTAAACGG GGTCTGCAGC
            241 CTGGCTGAGG AGCTGCGCCA CATCCACTCC AGGTACCGGG GCAGCTACTG GAGGACTGTG
            301 CGGGCCAGCC TGGGCAGCCC CCTCCGCCGT GGGGCCCTGT TGCTGCTGTC CATCTATTTC
            361 TACTACTCCC TCCCAAATGC GGTCGGCCCG CCCTTCACTT GGATGCTTGC CCTCCTGGGC
            421 CTCTCGCAGG CACTGAACAT CCTCCTGGGC CTCAAGGGCC TGGCCCCAGC TGAGATCTCT
            481 GCACTGTGTG AAAAAGGGAA TTTCAACGTG GCCCATGGGC TGGCATGGTC ATATTACATC
            541 GGATATCTGC GGCTGATCCT GCCAGAGCTC CAGGCCCGGA TTCGAACTTA CAATCAGCAT
            601 TACAACAACC TGCTACGGGG TGCAGTGAGC CAGCGGCTGT ATATTCTCCT CCCATTGGAC
            661 TGTGGGGTGC CTGATAACCT GAGTATGGCT GACCCCAACA TTCGCTTCCT GGATAAACTG
            721 CCCCAGCAGA CCGGTGACCA TGCTGGCATC AAGGATCGGG TTTACAGCAA CAGCATCTAT
            781 GAGCTTCTGG AGAACGGGCA GCGGGCGGGC ACCTGTGTCC TGGAGTACGC CACCCCCTTG
            841 CAGACTTTGT TTGCCATGTC ACAATACAGT CAAGCTGGCT TTAGCCGGGA GGGATAGGCT
            901 TGAGCAGGCC AAACTCTTCT GCCGGACACT TGAGGACATC CTGGGCAGAT GCCCCTGAGT
            961 CTCAGAACAA CTGCCCGCCC TCATTTGCCC TACCAGGAAC CTGCAGATGA CAGCAGCTTC
           1021 TCGCTGTCCC AGGAGGGTCT CCGGCACCTG CGGGCAAGGA GGAAAAAGGG AGAGGTTACT
          1081 GGTGGGGCAG CCTGAGACCT TCAGCGGGTG GCCAGTAACC CTTCAACGAA ATGGATTC
          //
          Primer: PuroR-rev
          Sequence file: RDB14356_A6J7b.seq check
          >D03866A6_A6J7_1_PuroR-rev_F06_06_ABI08.ab1
              1 AATGGACTTT TTGCAAAGCC TAGGCCTCCA AAAAGCCTCC TCACTACTTC TGGAATAGCT
             61 CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG
            121 GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA
            181 TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG
            241 ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG
            301 GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGGGTC GAGTCAAGAG
            361 AAATCCGTGC GGAGAGGGAG GGGCTTTTCC ATTCCACTGA TGAGGAGCTC AGGCTCTTGG
            421 GACATCGTGG AGGTACTGGG CACCGCTGAG GTCTTCAAGC TGCCCACAGT AACCTCTTCC
            481 TTTTCCTCCT GCCGCAGGTG CCGGAGAACC TCCTGGGACA GCGAGAAGCT GCTGTCATCT
            541 GCAGGTTCCT GGTAGGCAAT GAGGCGGCAG TTGTTCTGAG ACTCAGGGGC ATCTGCCAGG
            601 ATGTCCTCAA GTGTCCGGCA GAAGAGTTTG GCCTGCTCAA GCCTATCCTC CCGGCTAAAG
            661 CCAGCTTGAC TGTATTGTGA CATGGCAAAC AAAGTCTGCA AGGGGGTGGC GTACTCCAGG
            721 ACACAGGTGC CCGCCCGCTG CCCGTTCTCC AGAAGCTCAT AGATGCTGTT GCTGTAAACC
            781 CGATCCTTGA TGCCAGCATG GTCACCGGTC TGCTGGGGCA GTTTATCCAG GAAGCGAATG
            841 TTGGGGTCAG CCATACTCAG GTTATCAGGC ACCCCACAGT CCAATGGGAG GAGAATATAC
            901 AGCCGCTGGC TCACTGCACC CCGTAGCAGG TTGTTGTAAT GCTGATTGTA AGTTCGAATC
            961 CGGGCCTGGA GCTCTGGCAG GATCAGCCGC AGATATCCGA TGTAATATGA CCATGCCAGC
           1021 CCATGGGCCA CGTTGAAATT TCCCTTTTTC AACACAGTGC AGAGATCTCA GCTGGGGCCC
           1081 AGGCCCTTTG AGCCCAGAAG ATGGTCAGTG CCTGCGAGAG CTAGGAGCAG CCATTCAGTG
          1141 AGGCGGGCCG ACCCGCAATT TGGGGAA
          //
          Primer: Amp_R
          Sequence file: RDB14356_A6J7c.seq check
          >D03866A6_A6J7_1_Amp_R_F03_18_ABI24.ab1
              1 TTCTTTTTTA AAATTAATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA
             61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG
            121 CCACCTGCAG CCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG
            181 CTCAGGGCCA AGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG
            241 CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCAGCCCTCA
            301 GCAGTTTCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG
            361 TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC
            421 CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGGGC GCCAGTCCTC CGATTGACTG
            481 AGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA GTTGCATCCG ACTTGTGGTC
            541 TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA CCCGTCAGCG GGGGTCTTTC
            601 ATTTGGGGGC TCGTCCGGGA TCGGGAGACC CCTGCCCAGG GACCACCGAC CCACCACCGG
            661 GAGGCAAGCT GGCCAGCAAC TTATCTGTGT CTGTCCGATT GTCTAGTGTC TATGACTGAT
            721 TTTATGCGCC TGCGTCGGTA CTAGTTAGCT AACTAGCTCT GTATCTGGCG GACCCGTGGT
            781 GGAACTGACG AGTTCTGAAC ACCCGGCCGC AACCCTGGGA GACGTCCCAG GGACTTTGGG
            841 GGGCCGTTTT TGTGGCCCGA CCTGAGGAAG GGAGTCGATG TGGAAATCCG ACCCCCGTCA
            901 GGATATGTGG TTCTGGTAGG AGACGAGAAA CCTTAAAACA GTTCCCGCCC TCCGTTCTGA
            961 ATTTTTTGCT TTTCGGTTTG GGAAACCGAA AGCCCGCGCG TCTTTGTCTT GCTTGCCAGC
           1021 GCTGCAGCAT CGTTTCTGGT GTTGTCTCTG TCTGAACTGT GTTTCCTTGT AATTGTCTTG
          1081 AAATTAGGCA GACCTGTTAA CCACCTCCCC TTAAGTTTTG GAACTGTATA AG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB14356_A6J7d.seq check
          >D03866A6_A6J7_1_SV40pro_F_V2_F05_17_ABI24.ab1
              1 GGCCAACTTT TTTGGGAGCT AGGCTTTTGC AAAAGCTTAC CATGACCGAG TACAAGCCCA
             61 CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC GCCGCCGCGT
            121 TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC GAGCGGGTCA
            181 CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG
            241 CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG
            301 TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC
            361 AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA
            421 CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG
            481 GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA
            541 ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG
            601 GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG ACGCCCGCCC CACGACCCGC
            661 AGCGCCCGAC CGAAAGGAGC GCACGACCCC ATGCATCGAT AAAATAAAAG ATTTTATTTA
            721 GTCTCCAGAA AAAGGGGGGA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG
            781 TAACGCCATT TTGCAAGGCA TGGAAAAATA CATAACTGAG AATAGAGAAG TTCAGATCAA
            841 AGTCAGGAAC AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT
            901 CCTGCCCCGG CTCAGGGCCA AGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT
            961 CTGTGGTAAG CAGTTCCCTG CCCCGGCTCA AGGCCCAAGA ACAGATGGGT CCCCAGATGC
           1021 GGTCCAGGCC TCAGCCAGTT TTCTAGGAGA ACCAATCAGA TGGTTTCAGG ATGCCCCGAA
          1081 GGACTGAATT GACCTGATGC CTTATTTGAA CA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Mukai, K., Activation of STING requires palmitoylation at the Golgi. Nat. Commun. 7: 11932 (2016). PMID 27324217.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl