Please review the QC test results indicated by icon below as well as clone information before placing your order.

pBabe-hSTING(V155M)

Retroviral expression vector of human STING, V155M mutant.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Mukai, K. et al., Nat. Commun., 7:11932, 2016)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Mukai, K. et al., Nat. Commun., 7:11932, 2016)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14355_A6J7p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pBABE5' Sequence file: RDB14355_A6J7a.seq
>D03866A5_A6J7_1_pBABE5'_E01_13_ABI24.ab1     1 ATTGGGCCGG GAATCCAGTT GTGGTGGTAC GGTAGGAATT CGCCGCCACC ATGCCCCACT    61 CCAGCCTGCA TCCATCCATC CCGTGTCCCA GGGGTCACGG GGCCCAGAAG GCAGCCTTGG   121 TTCTGCTGAG TGCCTGCCTG GTGACCCTTT GGGGGCTAGG AGAGCCACCA GAGCACACTC   181 TCCGGTACCT GGTGCTCCAC CTAGCCTCCC TGCAGCTGGG ACTGCTGTTA AACGGGGTCT   241 GCAGCCTGGC TGAGGAGCTG CGCCACATCC ACTCCAGGTA CCGGGGCAGC TACTGGAGGA   301 CTGTGCGGGC CTGCCTGGGC TGCCCCCTCC GCCGTGGGGC CCTGTTGCTG CTGTCCATCT   361 ATTTCTACTA CTCCCTCCCA AATGCGGTCG GCCCGCCCTT CACTTGGATG CTTGCCCTCC   421 TGGGCCTCTC GCAGGCACTG AACATCCTCC TGGGCCTCAA GGGCCTGGCC CCAGCTGAGA   481 TCTCTGCAGT GTGTGAAAAA GGGAATTTCA ACATGGCCCA TGGGCTGGCA TGGTCATATT   541 ACATCGGATA TCTGCGGCTG ATCCTGCCAG AGCTCCAGGC CCGGATTCGA ACTTACAATC   601 AGCATTACAA CAACCTGCTA CGGGGTGCAG TGAGCCAGCG GCTGTATATT CTCCTCCCAT   661 TGGACTGTGG GGTGCCTGAT AACCTGAGTA TGGCTGACCC CAACATTCGC TTCCTGGATA   721 AACTGCCCCA GCAGACCGGT GACCATGCTG GGCATCAAGG ATCGGGTTTA CAGCAACAGC   781 ATCTAATGAG CTTCTGGAGA ACGGGGCAGC GGGCGGGCAC CTGTGTCCTG GAGTACGCCA   841 CCCCCCTTGC AGACTTTGTT TGCCATGTCA CAATACAGTC AAGCTGGCCT TTAGCCCGGG   901 AAGGATAAGG GCTTGAGCCA GGCCCAAAAC TCTTCTGGCC GGGACACTTT GAGGAACATT   961 CCTTGCCAGA ATGCCCCCTG AAAGTCTCAG AAAAAACTGG CCCGCCCTCC ATTTGCCCTA  1021 CCAGGGAAAC CTTGCAAGAA GGAACAGCCA GCCTTTCCTC GCCTTGTCCC CAAGAAGGGT  1081 TTCCTCCCGG CCACCTTGCC GGGCAGGGGA GGAAAAAGGA GAAGAAAGGT TTACTTGGTG  1141 TGGGGGCAAG ACCTTGGAAA GAAACCCCTT CAAGCGGGGT GTGCCCCCAG AGTATCACTC 1201 TCTCTCCAAC GAGAAGTATG TATTGTCCTC //
Primer: PuroR-rev Sequence file: RDB14355_A6J7b.seq
>D03866A5_A6J7_1_PuroR-rev_E06_05_ABI08.ab1     1 GCTGGACTTT TTGCAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT    61 CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG   121 GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA   181 TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG   241 ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG   301 GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT CCACAGGGTC GAGTCAAGAG   361 AAATCCGTGC GGAGAGGGAG GGGCTTTTCC ATTCCACTGA TGAGGAGCTC AGGCTCTTGG   421 GACATCGTGG AGGTACTGGG CACCGCTGAG GTCTTCAAGC TGCCCACAGT AACCTCTTCC   481 TTTTCCTCCT GCCGCAGGTG CCGGAGAACC TCCTGGGACA GCGAGAAGCT GCTGTCATCT   541 GCAGGTTCCT GGTAGGCAAT GAGGCGGCAG TTGTTCTGAG ACTCAGGGGC ATCTGCCAGG   601 ATGTCCTCAA GTGTCCGGCA GAAGAGTTTG GCCTGCTCAA GCCTATCCTC CCGGCTAAAG   661 CCAGCTTGAC TGTATTGTGA CATGGCAAAC AAAGTCTGCA AGGGGGTGGC GTACTCCAGG   721 ACACAGGTGC CCGCCCGCTG CCCGTTCTCC AGAAGCTCAT AGATGCTGTT GCTGTAAACC   781 CGATCCTTGA TGCCAGCATG GTCACCGGTC TGCTGGGGCA GTTTATCCAG GAAGCGAATG   841 TTGGGGTCAG CCATACTCAG GTTATCAGGC ACCCCACAGT CCAATGGGAG GAGAATATAC   901 AGCCGCTGGC TCACTGCACC CCGTAGCAGT TGTTGTAATG CTGATTGTAA GTTCGAATCC   961 CGGGCCTGGA GCTCTGGCAG GATCAGCCGC AGATATCCGA TGTAATATGA CCATGCCAGC  1021 CCATGGGCCC ATGTTGAAAT TTCCCTTTTT CCACACACTG CAGAGATCTC AGCTGGGGCC  1081 AGGGCCCATG AGCCCAGAGG ATGTTCAGTG CTTGCAGAGC CAAGGAGCCA AGCATCCAAG 1141 TGCAAGGCCG GGTTCCGAAC CTGCCT //
Primer: Amp_R Sequence file: RDB14355_A6J7c.seq
>D03866A5_A6J7_1_Amp_R_E03_15_ABI24.ab1     1 ATCTTTTTTT TCATTATTAT TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC    61 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA   121 GTGCCACCTG CAGCCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA GTTCCTGCCC   181 CGGCTCAGGG CCAAGAACAG ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT   241 AAGCAGTTCC TGCCCCGGCT CAGGGCCAAG AACAGATGGT CCCCAGATGC GGTCCAGCCC   301 TCAGCAGTTT CTAGAGAACC ATCAGATGTT TCCAGGGTGC CCCAAGGACC TGAAATGACC   361 CTGTGCCTTA TTTGAACTAA CCAATCAGTT CGCTTCTCGC TTCTGTTCGC GCGCTTCTGC   421 TCCCCGAGCT CAATAAAAGA GCCCACAACC CCTCACTCGG GGCGCCAGTC CTCCGATTGA   481 CTGAGTCGCC CGGGTACCCG TGTATCCAAT AAACCCTCTT GCAGTTGCAT CCGACTTGTG   541 GTCTCGCTGT TCCTTGGGAG GGTCTCCTCT GAGTGATTGA CTACCCGTCA GCGGGGGTCT   601 TTCATTTGGG GGCTCGTCCG GGATCGGGAG ACCCCTGCCC AGGGACCACC GACCCACCAC   661 CGGGAGGCAA GCTGGCCAGC AACTTATCTG TGTCTGTCCG ATTGTCTAGT GTCTATGACT   721 GATTTTATGC GCCTGCGTCG GTACTAGTTA GCTAACTAGC TCTGTATCTG GCGGACCCGT   781 GGTGGAACTG ACGAGTTCTG AACACCCGGC CGCAACCCTG GGGAGACGTC CCAGGGACTT   841 TGGGGGGCCG TTTTTGTGGC CCGACCTGAG GAAGGGGAGT CGATGTGGGA ATCCGACCCC   901 GTCAGGGATA TGTGGGTTTC TGGGTAGGAG ACGAGAACCT AAAAACAGTT TCCGCCCTCC   961 CGTCTGGAAT TTTTTGCTTT TCGGGTTTGG AACCGAAGCC GCGCGTCTTG TTCTGCCTGC  1021 AGCGCTTGCA GCAATCGATC TGGTGGTTGG TTCATCTGGT CTGAACTGGT GGTTTTCTGG  1081 TATTTTGGTC CTGGAAAATT AAGGGCCAGG ACATGTGTAA CCACTTCCCT TAGTATTTGG 1141 AACCCTTAGT CACTGGGAAA AGAATGTTCA GACGGATTTC GCCTTCTCGA GCA //
Primer: SV40pro_F_V2 Sequence file: RDB14355_A6J7d.seq
>D03866A5_A6J7_1_SV40pro_F_V2_E05_14_ABI24.ab1     1 GACAACTTTT TTGGAGGCCT AGGCTTTTGC AAAAGCTTAC CATGACCGAG TACAAGCCCA    61 CGGTGCGCCT CGCCACCCGC GACGACGTCC CCAGGGCCGT ACGCACCCTC GCCGCCGCGT   121 TCGCCGACTA CCCCGCCACG CGCCACACCG TCGATCCGGA CCGCCACATC GAGCGGGTCA   181 CCGAGCTGCA AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG   241 CGGACGACGG CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG   301 TGTTCGCCGA GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC   361 AACAGATGGA AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA   421 CCGTCGGCGT CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG   481 GAGTGGAGGC GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA   541 ACCTCCCCTT CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG   601 GACCGCGCAC CTGGTGCATG ACCCGCAAGC CCGGTGCCTG ACGCCCGCCC CACGACCCGC   661 AGCGCCCGAC CGAAAGGAGC GCACGACCCC ATGCATCGAT AAAATAAAAG ATTTTATTTA   721 GTCTCCAGAA AAAGGGGGGA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG   781 TAACGCCATT TTGCAAGGCA TGGAAAAATA CATAACTGAG AATAGAGAAG TTCAGATCAA   841 GGTCAGGAAC AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT   901 CCTGCCCCGG CTCAGGGCCA AGAACAGATG GAACAGCTGA AATATGGGCC CAAACAGGGA   961 TATCTGTGGT AAGCAGTTCC TGCCCCCGGG CTCAAGGGCC CAAGACAGAT GGGTCCCCAG  1021 ATGCGGTTCC AGCCTCAGCA GTTTTCTAGG AGGAACATCA GATGGTTTTC CCAGGGTGCC 1081 CCAAGGACCT GGAATGGACC TGTTGCTTAT TTTGAAACCT TAACCA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl