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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCAGGS/hBACE1 N153/223S

Expression vector of human BACE1mutant, N153S/N223S.

Catalog number RDB14348
Resource name pCAGGS/hBACE1 N153/223S
Alternative name pCAGGS/human BACE1 Asn153,223Ser
Clone info. Expression vector of human BACE1mutant, N153S/N223S. Point mutations at two N-glycosylation sites (Asn153 and Asn223) abolish the bisecting GlcNAc modification on BACE1.
Comment Expression was confirmed by the depositor with western blotting and immunoprecipitation.
Vector backbone pCAGGS (plasmid)
Selectable markers Amp^r
Gene/insert name human BACE1 cDNA
Depositor|Developer Taniguchi, Naoyuki | Kizuka, Yasuhiko |
Other clones in our bank

External Database
human BACE1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)
          Additional terms and conditions for distribution Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)。
          付加的提供条件 CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB14348 pCAGGS/hBACE1 N153/223S DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14348_A6Itp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pAxCA_F1
          Sequence file: RDB14348_A6Ita.seq check
          >D03881A6_A6It_2_pAxCA_F1_D11_04_ABI08.ab1
              1 GAAAACCCTG ACTTGCTACA TGTTCATGCC TTCTTCTTTT TCCTACAGCT CCTGGGCAAC
             61 GTGCTGGTTA TTGTGCTGTC TCATCATTTT GGCAAAGAAT TCATGGCCCA AGCCCTGCCC
            121 TGGCTCCTGC TGTGGATGGG CGCGGGAGTG CTGCCTGCCC ACGGCACCCA GCACGGCATC
            181 CGGCTGCCCC TGCGCAGCGG CCTGGGGGGC GCCCCCCTGG GGCTGCGGCT GCCCCGGGAG
            241 ACCGACGAAG AGCCCGAGGA GCCCGGCCGG AGGGGCAGCT TTGTGGAGAT GGTGGACAAC
            301 CTGAGGGGCA AGTCGGGGCA GGGCTACTAC GTGGAGATGA CCGTGGGCAG CCCCCCGCAG
            361 ACGCTCAACA TCCTGGTGGA TACAGGCAGC AGTAACTTTG CAGTGGGTGC TGCCCCCCAC
            421 CCCTTCCTGC ATCGCTACTA CCAGAGGCAG CTGTCCAGCA CATACCGGGA CCTCCGGAAG
            481 GGTGTGTATG TGCCCTACAC CCAGGGCAAG TGGGAAGGGG AGCTGGGCAC CGACCTGGTA
            541 AGCATCCCCC ATGGCCCCAG CGTCACTGTG CGTGCCAACA TTGCTGCCAT CACTGAATCA
            601 GACAAGTTCT TCATCAACGG CTCCAACTGG GAAGGCATCC TGGGGCTGGC CTATGCTGAG
            661 ATTGCCAGGC CTGACGACTC CCTGGAGCCT TTCTTTGACT CTCTGGTAAA GCAGACCCAC
            721 GTTCCCAACC TCTTCTCCCT GCAGCTTTGT GGTGCTGGCT TCCCCCTCAG CCAGTCTGAA
            781 GTGCTGGCCT CTGTCGGAGG GAGCATGATC ATTGGAGGTA TCGACCACTC GCTGTACACA
            841 GGCAGTCTCT GGTATACACC CATCCGGCGG GAGTGGTATT ATGAGGTGAT CATTGTGCGG
            901 GTGGAGATCA AATGGACAGG ATCTGAAAAT GGACTGCAAG GAGTACAACT ATGACAAGAG
            961 CATTGTGGAC AGTGGCACCA CCAACCTTCG TTTGCCCAAG AAAGTGTTTG AAGCTGCAGT
           1021 CAAATCCATC AAAGGCCAGC CTTCCTCCAC GGGAGAGTTT CCCTGATGGG TTTTCTGGCT
           1081 AGGAGAGCAG CTGGTGTGCT GACAAGGCAG CCACCAACCC TGGACCATTT TTCCAGTCAA
          1141 TCCTCACTCT ACCTTAAATG GGGTTGAAGG GTTACCA
          //
          Primer: pCA-bgter
          Sequence file: RDB14348_A6Itb.seq check
          >D03881A6_A6It_2_pCA-bgter_B11_05_ABI24.ab1
              1 AGCCCCCAGT AATGTCCCAG AATTTTTGGC AGAGGGAAAA AGATCTCAGT GGTATTTGTG
             61 AGCCAGGGCA TTGGCCACAC CAGCCACCAC CTTCTGATAG GCAGCCTGCA CCTGAGGAGT
            121 GAATTCTCAC TTCAGCAGGG AGATGTCATC AGCAAAGTCA TCATGCTGCT GGCGCAGGCA
            181 GCGGAGGCAG CGCCACTGAC ACACCATGAG GCAGAGTGGC AGCATGAAGA GGGCGCAGAT
            241 GGCAGCCATG ACATAGGCTA TGGTCATGAG GGTTGACTCA TCTGTCTGTG GAATGTTGTA
            301 GCCACAGTCT TCCATGTCCA AGGTGACAAA AGGGCCTTCC ACCGCTGCCG TCCTGAACTC
            361 ATCGTGCACA TGGCAAGCGC TGACAGCAAA GCCAATTCGT TTTCGGGCCC GATCAAAGAC
            421 AACGTAGAAG CCCTCCATGA TAACAGCTCC CATAACAGTG CCCGTGGATG ACTGTGAGAT
            481 GGCAAACTTG TAACAGTCGT CTTGGGACGT GGCCACATCT TCCACTGGCC GCAGGTATTG
            541 CTGCGGAAGG ATGGTGATGC GGAAGGACTG GTTGGTAACC TCACCCATTA GGTAGAGTGA
            601 GATGACTGGG AAAATGTTCC AAGGGGTGGT GCCTGCTTGC CAGCACACCA GCTGCTCTCC
            661 TAGCCAGAAA CCATCAGGGA ACTTCTCCGT GGAGGAGGCT GCCTTGATGG ATTTGACTGC
            721 AGCTTCAAAC ACTTTCTTGG GCAAACGAAG GTTGGTGGTG CCACTGTCCA CAATGCTCTT
            781 GTCATAGTTG TACTCCTTGC AGTCCATTTT CAGATCCTGT CCATTGATCT CCACCCGCAC
            841 AATGATCACC TCATAATACC ACTCCCGCCG GATGGGTGTA TACCAGAGAC TGCCTGTGTA
            901 CAGCGAGTGG TCGATACCTC CAATGATCAT GCTCCCTCCG ACAGAGGCCA GCACTTCAGA
            961 CTGGGCTGAG GGGGAAGCCA GCACCACAAA GCTGCAGGGA GAAGAGGTTG GGAACGTGGG
           1021 TCTGCTTTAC CAGAGAGTCA AAGAAAGGCT CCAGGGGAGT CGTCAAGGCC TGCAATCTCA
           1081 GCATAGCCAG CCCCAAGATG CGTCCAGTGG AGCCGTGGAT GAGACTGTCT GAATTCAGTG
          1141 ATGCAGCCAA TGGTGGCACG CCAACAAGTG AACCGCCTTG GGGCCCATTG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl