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pGEX4T3-mouse MICAL-L2-C - active Rab8/10 trapper

Bacteria expression vector of C-terminal fragment of mouse MICAL-L2.

Catalog number RDB14285
Resource name pGEX4T3-mouse MICAL-L2-C - active Rab8/10 trapper
Clone info. A C-terminal fragment of mouse MICAL-L2 is introduced into pGEX4T3 which is a GST fusion protein expression vector. GST-MICAL-L2-C works as a trapper of the GTP binding-active forms of Rab8/10.
Comment Expression was confirmed by the depositor with pull down assay. Known differences compared with NM_ 174850.3: C to T (Ile synonym) at 2696nt and T to A (Gly synonym) at 2765nt. Note: This clone has lacI instead of lacIq promoter.
Vector backbone pGEX-4T-3 (Plasmid)
Size of vector backbone 5.0 kb
Selectable markers Amp^r
Gene/insert name mouse MICALL2 cDNA
Depositor|Developer Fukuda, Mitsunori |
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External Database
mouse MICALL2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。
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          RDB14285 pGEX4T3-mouse MICAL-L2-C - active Rab8/10 trapper DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14285_A6H4p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX-RSEQ
          Sequence file: RDB14285_A6H4a.seq check
          >14285_14285_A9EeF_pGEX-RSEQ_A06_01_ABI08.ab1
              1 TCCAATCCCG GAAAAGCGCG AGGCAGATCG TCAGTCAGTC ACGATGCGGC CGCGGGAATT
             61 CGATTTCAGG CCTGCCCGCT TTTGCTCTTC GAGGACCAGA TCTTGGACAA GAAGGACTTG
            121 GACTTCTTTC TCTGGAGGCC CAGGTTCTGG ATCATATTCT CCAGCATTTG GTCCTCTTCC
            181 TGTTCCCGGA GCCGATCCTC ATCCAGGAAG TCAACGATGT CACTTCGGTC ATTCACGGTG
            241 TTCACGTATT GGCTCAGCAG CTCCTGCTCC CGCTGGCGGT CCTGGGGGGA CTTCAGACCC
            301 TCTGGCTTGT CCATCAGCCT GCGCAGCTCA CCCTGGAGGT CCAGCTGCTG CTCCTCCAGG
            361 CGCTGGTCCT TGGACTTGTA CATGAGTTCC GATTCCAGCC TCAACAGTAG CTGCTTCTCA
            421 TGAATAAGCC GGAACCAGTC CACCATGAGG CTATCCTCAG AAGCATCTCC CTCAGCTGCC
            481 CGCAGCCGCT TCTCCAACTC AACTCCCCTG AGCTCCAGGG CGTCCAGTTG GCTCTCAATG
            541 TCCTGCAGCT GCCTCTGCAG CTCCTCCTGG GGAATATAGT CGGGGTGGAG CCTGACTGGG
            601 GAAGTGACAG CTTCACCAGG TGGAGCAAGG CCACTGTCCA GGACTGCAGA CCTCTCTCTT
            661 GACCACCTGG TGGGTGACTT GGATCCACCA CCACCACCGG AAATTCCCGG AGATCCACGC
            721 GGAACCAGAT CCGATTTTGG AGGATGGTCG CCACCACCAA ACGTGGCTTG CCAGCCCTGC
            781 AAAGGCCATG CTATATACTT GCTGGATTTC AAGTACTTAT CAATTTGTGG GATAGCTTCA
            841 ATACGTTTTT TAAAACAAAC TAATTTTGGG AACGCATCCA GGCACATTGG GTCCATGTAT
            901 AAAACAACAT CAAGAGCGTC ATACAACATG AAGTCAGGAT GGGTTACATG ATCACCATTT
            961 AAATATGTTT TATGACATAA ACGATCTTTC GAACATTTTC AGCATTTCAG GTAGCTTTGC
           1021 TAAGAAAATC AACTTTGAGA GTTTCAAAGT CTTTACTATA TGCAATTTCT CGAAACACGT
           1081 ATCTAATAAT CCAAAACCCG CTCCCTCAGG CAATGAATCT TCTGCACGCC CTCTTTTGGA
          1141 CAAACCTCAC CTCCACAAAC AGTATGTGTG
          //
          Primer: PGEX-ptac_up-F
          Sequence file: RDB14285_A6H4b.seq check
          >14285_14285_A9EeF_pGEX-ptac_up_F_A12_01_ABI08.ab1
              1 TGCCCACCAT TCATAAACGG GTTCCTGGCA AATATTCTGA AATGAGCTGT TGACAATTAA
             61 TCATCGGCTC GTATAATGTG TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGT
            121 ATTCATGTCC CCTATACTAG GTTATTGGAA AATTAAGGGC CTTGTGCAAC CCACTCGACT
            181 TCTTTTGGAA TATCTTGAAG AAAAATATGA AGAGCATTTG TATGAGCGCG ATGAAGGTGA
            241 TAAATGGCGA AACAAAAAGT TTGAATTGGG TTTGGAGTTT CCCAATCTTC CTTATTATAT
            301 TGATGGTGAT GTTAAATTAA CACAGTCTAT GGCCATCATA CGTTATATAG CTGACAAGCA
            361 CAACATGTTG GGTGGTTGTC CAAAAGAGCG TGCAGAGATT TCAATGCTTG AAGGAGCGGT
            421 TTTGGATATT AGATACGGTG TTTCGAGAAT TGCATATAGT AAAGACTTTG AAACTCTCAA
            481 AGTTGATTTT CTTAGCAAGC TACCTGAAAT GCTGAAAATG TTCGAAGATC GTTTATGTCA
            541 TAAAACATAT TTAAATGGTG ATCATGTAAC CCATCCTGAC TTCATGTTGT ATGACGCTCT
            601 TGATGTTGTT TTATACATGG ACCCAATGTG CCTGGATGCG TTCCCAAAAT TAGTTTGTTT
            661 TAAAAAACGT ATTGAAGCTA TCCCACAAAT TGATAAGTAC TTGAAATCCA GCAAGTATAT
            721 AGCATGGCCT TTGCAGGGCT GGCAAGCCAC GTTTGGTGGT GGCGACCATC CTCCAAAATC
            781 GGATCTGGTT CCGCGTGGAT CTCCGGGAAT TTCCGGTGGT GGTGGTGGAT CCAAGTCACC
            841 CACCAGGTGG TCAAAGAGAG AGGTCTGCAG TTCCTGGACA GTGGCCTTGC TCCACCTGGT
            901 GAAGCTGTCA CTTTCCCCAG TCAGGCTCCA CCCCGACTAT ATTCCCCAGG AAGGAGCTGC
            961 AGAGGCAGCT TGCAAGGACA ATTGAGAAGC CAACTGGACG CCCTGGAAGC TTCAGGGAAG
           1021 TTGAGTTGGG AAGAAGCCGG CTTGCTGTAG CTGAAGGAGA TGGCTTTCCT GAGGATAAGC
           1081 TCATGGGTTG GAAACTGGTT CCGGCTAATC ATTGAAAAGG CAGGCTACTG TGGAGGGCCT
          1141 GGAATTCTGG GACAACT
          //
          Primer: SV40pA3'element Reverse
          Sequence file: RDB14285_A6H4c.seq check
          >D03692B6_A6H4_1_SV40pA3'element_Reverse_D07_10_ABI24.ab1
              1 AATCGCCAGG GGGGGCGGAC CTATGGAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT
             61 GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA
            121 TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG
            181 CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA
            241 TCTGTGCGGT ATTTCACACC GCATAAATTC CGACACCATC GAATGGCGCA AAACCTTTCG
            301 CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG GTGGTGAATG TGAAACCAGT
            361 AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT
            421 GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA
            481 GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT
            541 TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA
            601 ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAAGCGGCGT
            661 CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT
            721 TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC
            781 GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC AGTATTATTT TTCTCCCATG
            841 AAGACGGTAC GCGACTGGGG CGTGGAGCAT CTGGTCGCAT TTGGGGTCAC CAGCAAATCG
            901 CGCTGTTAGC GGGCCCATTA AGTTCTGTCT CGGCGCGTCT GCGTCTGGCT GGCTGGCATA
            961 AATATCTCAC TCGCAATCAA AATTTCAGCC GGATAGCGGA CGGGGAAGGC GACTTGGGAG
           1021 GTTGCCCATT GTTCCGGTTT TTCAAACAAA CCAATGCCAA ATGGCTTGAA ATTGAAGGCA
          1081 ATCGATTCCC AACTGCGGAA TGGCTTGGAT TTGCCAACGT AATTTCAAGG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl