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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-FLAG-mouse Rab10

Expression vector of FLAG-tagged mouse Rab10.

Catalog number RDB14272
Resource name pEF-FLAG-mouse Rab10
Clone info. Expression vector driven by the EF1 promoter for FLAG-tagged mouse Rab10.
Comment Reference sequence is NM_016676. pEF-FLAG is delived from the pEF-BOS vector (J. Biol. Chem., 274 (44): 31421-31427., 1999. PMID 10531343) with FLAG-tag sequence at the upstream of the cloning site. Expression was confirmed by the depositor with immunoprecipitation.
Vector backbone pEF-FLAG (Plasmid)
Selectable markers Amp^r
Gene/insert name mouse Rab10 cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
mouse Rab10
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kuroda, T. S. et al., J. Biol. Chem. 277, 9212-9218, 2002); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kuroda, T. S. et al., J. Biol. Chem. 277, 9212-9218, 2002); (Homma, T. et al., Mol. Biol. Cell, 27, 2107-2118, 2016)。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14272 pEF-FLAG-mouse Rab10 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
          original Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Rab family protein (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14272_A6H4p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: hGCSF_R
          Region: hGCSF pA, insert, FLAG, EF-1 alpha pro
          Sequence file: RDB14272_A6H4a.seq check
          >D03692A2_A6H4_1_hGCSF_R_D01_10_ABI24.ab1
              1 CGTGGGAATG CAACTCTCAG GGTCCCAGGT GGGGACCCTC ACTCTAGAGC GGCCGCTATG
             61 GTCGACCTGC AGGCGGCCGC GAATTCACTA GTGATTTCAG CAGCACTTGC TCTTCCAGCC
            121 CGTCACGCCT CCTCCACTGC TGATATCTAC GTTTTCACTG TTGGGTTCTT TTACAGGGGT
            181 CTTTCGGAGG ATGTCTTCAG CTAATGTGAG GAACGCCTTT TCGATGTTTA TATTTGCTTT
            241 TGCACTAGTC TCAAAAAACC TAATACCATG CTCCCTTGCA ATCTGTTCTC CTTTGCCTTT
            301 CGGTACAACT CTCTTGTCGT CCATGTCACA CTTGTTCCCT AGTAACATTC TTTCCACATC
            361 TTCATTGGCA TGCTCATCTA TGTTTCTAAG CCACTTGCTG ATGTTCTCAA AGCTTTTACC
            421 GTTGGTGATG TCATACACTA GCATGATGCC CATTGCTCCT CTGTAGTAGG AGGTTGTGAT
            481 GGTGTGAAAT CGCTCCTGGC CTGCTGTGTC CCATATCTGT AGCTTGATCT TCTTTCCTTG
            541 TAGTTCCACT GTTTTGATCT TAAAGTCTAT TCCTATGGTG GAAATAAAGG TGGTATTGAA
            601 GGCATCGTCC GAAAAACGAA AAAGGACGCA GGTCTTGCCC ACTCCCGAGT CCCCGATCAG
            661 GAGCAGCTTG AAAAGCAGGT CGTACGTCTT CTTCGCCATG GATCCCTTGT CGTCATCGTC
            721 CTTGTAGTCC ATGGTGGAAT CGAATTCCCG CGGCCGCTCT AGAGAATTCC TCACGACACC
            781 TGAAATGGAA GAAAAAAACT TTGAACCACT GTCTGAGGCT TGAGAATGAA CCAAGATCCA
            841 AACTCAAAAA GGGCAAATTC CAAGGAGAAT TACATCAAGT GCCAAGCTGG CCTAACTTCA
            901 GTCTCCACCC ACTCAGTGTG GGGAAACTCC ATCGCATAAA ACCCCTCCCC CCCAACCCTA
            961 AAGACGACGT ACTCCAAAAG CTCGAGAACT AATCGAGGTG CCCTGGACGG CGCCCCGGTA
           1021 CTCCGTGGAG TCACATGGAA GCGACGCTGG AGACGGAAAG CCTTTTTCCT TTGTGTGGTG
          1081 ACTCACGAGC GCTCTCCGAA GCGCCGCGAT CATTCAATTT GGAGGCCTCC CCG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Homma, Y., Rabin8 regulates neurite outgrowth in both GEF activity-dependent and -independent manners. Mol. Biol. Cell, 27 (13): 2107-2118 (2016). PMID 27170183.
          original Kuroda, T.S., The Slp homology domain of synaptotagmin-like proteins 1-4 and Slac2 functions as a novel Rab27A binding domain. J. Biol. Chem., 277, 9212-9218 (2002). PMID 11773082.

          2022.05.17

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