Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA5-FLAG/HA/SBP-TNRC6A

Expression vector of FLAG/HA/SBP-tagged human TNRC6A.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nishi, K. et al., RNA, 19 (1): 17-35, 2013). This MATERIAL is distributed to nonprofit organizations for research and teaching purpose only.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nishi, K. et al., RNA, 19(1):17-35, 2013)。教育研究目的での使用に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14219_A6Fup1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB14219_A6Fua.seq
>D03279A7_A6Fu_1_CMV-Forward_A10_01.ab1     1 ATATCACTCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG    61 CAGAGCTCTC CCTATCAGTG ATAGAGATCT CCCTATCAGT GATAGAGATC GTCGACGAGC   121 TCGTTTAGTG AACCGTCAGA TCGCCTGGAG ACGCCATCCA CGCTGTTTTG ACCTCCATAG   181 AAGACACCGG GACCGATCCA GCCTCCGGAC TCTAGCGTTT CCACCGCGGT GGCGGCCGCC   241 ACCATGGATT ACAAGGATGA CGACGACGAT AAGTACCCAT ACGATGTTCC AGATTACGCT   301 TCTATGGACG AGAAGACCAC CGGCTGGCGG GGCGGCCACG TGGTGGAGGG CCTGGCCGGC   361 GAGCTGGAGC AGCTGCGGGC CAGGCTGGAG CACCACCCTC AGGGCCAGCG GGAGCCCTGC   421 CCGGGCGGAT CCATGGATGC TGATTCTGCC TCCAGTTCTG AATCAGAGAG AAACATCACT   481 ATCATGGCTT CAGGGAACAC AGGTGGTGAA AAAGATGGCC TTCGGAATAG CACTGGACTT   541 GGTTCCCAAA ACAAGTTTGT AGTTGGTAGC AGCAGCAATA ATGTGGGCCA TGGAAGTAGT   601 ACTGGGCCAT GGGGTTTTTC CCATGGAGCC ATAATAAGCA CATGTCAGGT CTCTGTGGAT   661 GCTCCTGAAA GCAAATCTGA AAGTAGCAAC AATAGAATGA ATGCTTGGGG CACTGTAAGT   721 TCTTCATCAA ATGGAGGGTT AAATCCAAGC ACTTTGAATT CAGCTAGCAA CCATGGTGCC   781 TGGCCAGTAT TAGAGAACAA TGGACTTGCC CTAAAAGGGC CTGTAGGGAG TGGTAGTTCT   841 GGCATTAATA TTCAGTGCAG TACTATAGGC CAGATGCCTA ACAATCAGAG TATTAACTCT   901 AAAGTGAGTG GTGGGTTCTA CCCATGGTAC CTGGGGAAAG CCCTTTCAGA AACTTTGTGA   961 AATCTGAAGT AAGGTGGGTA CACAGAAGTT TCCAATTCCA GTTGATCCAA ACCTCAAAAT 1021 ATTAACTACT TGGAATTGGA CTGGATCGAA TAACACTTAC CTAACCTTTT TATTG //
Primer: TNRC_NES-NLS-GW3 Sequence file: RDB14219_A6Fub.seq
>D03279A7_A6Fu_1_TNRC6A_NES-NLS-GW3_B10_04.ab1     1 ACTGAGGCTG GGCTGATGAT CTTGCATGGG AGCTTCATGC TTTGACTGGA CATAAACTGC    61 ATGTTTTGGG ATTCATCTGC TACAATGCCA TCCTTATCAA AATAAGGATT GCGCTCCATG   121 GAAGACTCTT TGGAAATCTG AGGCCGAGAA CCAGGGCCTT TCCCGACCGT TCGATTGTAA   181 TCACCAATAT TTAGGCTATG TTTATCTATC TCCATTCGTT TGTCTTGTAA CATTCCTCCA   241 GAAAGGTCCA TCTTATTGCT TTGTACATTT TCCTCTGGTG AGTCTCTCGA AAAACTGATG   301 TTTGAAAACT GTTTAACAAA TGGATTTATC CACGCTTCTT CTTGTTTGTT TCCACCTTTC   361 ATCATTCCCC TTTCCCTTCT CCTTTTTTTG CCACTCAGAC CCTTCGATGA CATTTTCTTT   421 GTATATGGTG GCCAATTTAA AGATGAGTTA AGCTCTTGAG ATGAATTACT ATTCCACATT   481 CCAATCTCCA CATCCTCTTC CTCTTCCCAG CCAGTGGGGC GATTTCCAGG CAATGGCATA   541 TCATCACCAC ACCAGCCATC TTGCATAGAT TTTGGCCCAG ATTTGCTTAA TGCTTGTGGA   601 CCAACAGAGC TGGAGCCCCA CGTGGATGTG CTGGATGCCG CAGCAATGGG TTCCCCCCAG   661 CTGGGACCAC TGTCTATGGG CTTACCCCAT GCTGAAGTAC CATTATCCAC AGTTGTGGCT   721 GGAGTAGAAG GCTCCCCCCA GGGCTCACCC CAGCCAGAGC CACTGCTTGC CTCTTTGTTT   781 GAGATGGCAC TTGCAGGCGT TAGCAGCTGA TGTACCTGTG CTTGCTGGTC TGAACGGCTG   841 TTGCCATTTG GGACGTTTTT TGTTCCACAT GTTCACATTT TTTGTAGTTG TATTTGCTTG   901 GAATCTCCCC CAAGCTGAAG TTTCCATCAT CAATCTCCAA TTTTGCGACG TATAGATTCT   961 GGGGGATGGG TTTCCCTCCC AGCCTGTGGT TTCATCCTTC TTTTGCTTGG GGCTGGGTAT  1021 AGTCCCCCAG CAAGCCTGGT TACCAGAGGA TTTGCTGTAG CTGTTGGAAT CCCCCAAAGA 1081 AATCCA //
Primer: BGH_rev2 Sequence file: RDB14219_A6Fuc.seq
>D03279A7_A6Fu_1_BGH_rev2_C10_07.ab1     1 CAATTGGATA AGTAGCACGG GGGAGGGGCA ACAACAGATG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG ATCAGCGGGT TTCGAGTGAA TTGTAATACG ACTCACTATA GGGCGAATTG   121 GGTACACTTA CCTGGTACCT TAATTAATTA AGGTACCGGG CCCCCCCTCG AGGTCGACGG   181 TATCGATAAG CTTGATATCC TAGATTACAT GGACTCTCCA CCCCCACCCA GGTGGTCAAC   241 AGAAAGAAAA GCGTTAATGG GAGATGGGCT GCTAATTCCT CGGGGGTCGC TGCTGCTTGG   301 GGGACCCCAC AGGCTTGTGG AATAATGCGG GGTCCCCCAG AGTGAAGTGC CGTGAAGGTC   361 TCCACAGTTA GTTCCCGACA GCCCAGCACC ATTCCAGTGA TTGAGATCGG TCCGGCTGGA   421 GAATGAGTGG GAACAGTCGA GGGAGCCCAG CCGGCTCTGG CTGGACCCGA GAGACTGCCA   481 GCCGGGAGAA GGGGTCAGAG ACTGGCTTTG TGCAAAGAAA CGACTGATCT CCTCTTCACT   541 GGCAAACTCA GCAAGAATAG TAGTGTTCCC CAGTACACAC ATGTGCAGAG ACTTTTGTGC   601 CTTCACTACC TCTTCTTTTG AACTGTAGCG GACCAGAGCA TTTCCGTGAG GGAGGTTCAG   661 GTGGAATGTG ATCAGCGGGC CGTGCTGCAT GCACAGAGTG CGCAGAGTTG AGCCATCGAT   721 CTGAGGTGTA AGGTTTTTTT AGAACAAGCC AATTTGTTAT TCTCCCTGAG CTGCTCTCAC   781 CCCAGCTGGA ACCTGGGGGT ATATCTGGCG TCAGAATTTC CCCATCCCTC CACCTATACG   841 AAAGGGGAGA ATTATCCCAC GTAGACAAGG GTGGCTTTCT GACCAGTCAG TCCCGGGAGG   901 TGGGGCGGGA CGAGCAGTGA TGTTTTTTAG GTGGGCAAAG GACTTTCACA GCTCATGGAG   961 CCAGAAGGAG GTGTTTGTAA CTGACCAGAG ACATGTCATT GGATCACTAT TTCTGCCTGG 1021 ACTTGCTAGT GCTGTACTGC TTGAGGGAAG CCGGATG //
Primer: BGHpA_F Sequence file: RDB14219_A6Fud.seq
>D03279A7_A6Fu_1_BGHpA_F_D10_10.ab1     1 CGGGATGGTT ATCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG GAGGATTGGG    61 AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG GCGGAAAGAA   121 CCAGCTGGGG CTCTAGGGGG TATCCCCACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG   181 GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT   241 TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC   301 GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG   361 ATTAGGGTGA TGGTTCACGT ACCTAGAAGT TCCTATTCCG AAGTTCCTAT TCTCTAGAAA   421 GTATAGGAAC TTCCTTGGCC AAAAAGCCTG AACTCACCGC GACGTCTGTC GAGAAGTTTC   481 TGATCGAAAA GTTCGACAGC GTCTCCGACC TGATGCAGCT CTCGGAGGGC GAAGAATCTC   541 GTGCTTTCAG CTTCGATGTA GGAGGGCGTG GATATGTCCT GCGGGTAAAT AGCTGCGCCG   601 ATGGTTTCTA CAAAGATCGT TATGTTTATC GGCACTTTGC ATCGGCCGCG CTCCCGATTC   661 CGGAAGTGCT TGACATTGGG GAATTCAGCG AGAGCCTGAC CTATTGCATC TCCCGCCGTG   721 CACAGGGTGT CACGTTGCAA GACCTGCCTG AAACCGAACT GCCCGCTGTT CTGCAGCCGG   781 TCGCGGAGGC CATGGATGCG ATCGCTGCGG CCGATCTTAG CCAGACGAGC GGGTTCGGCC   841 CATTCGGACC GCAAAGGAAT CGGTCAATAC ACTACATGGC GTGATTTCAT ATGCGCGATT   901 GCTGATCCCC ATGTGTATCA CTGGCAAACT GTGATGGGAC GACACCGTCA GTGCGTCCGT   961 CGCGCAGCTC TCGATGAGCT GATGCTTTGG GCCGAAGACT GCCCCGAAGG TCAGCACCTC  1021 GTGCACGCGG AATTTCGCTC GACCATGTCT GACGGACATG ACGGCATAAC CAGCCGGGTT 1081 CAATTGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl