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pGL3_hBmalPro-Luc (-829_+57)

Reporter plasmid of human Baml 1 promoter.

Catalog number RDB14177
Resource name pGL3_hBmalPro-Luc (-829_+57)
Alternative name 10
Clone info. Expression vector of SV40 promoter driving a firefly luciferase with human Baml 1 promoter, hBmal1 region spans from -829 to +57 (+1 is the putative transcription start site).
Comment Expression was confirmed by the depositor with culture-based luminescent repoter assay.
Vector backbone pGL3-Basic vector (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name human Bmal 1 genomic
Depositor|Developer Takumi, Toru |
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External Database
human Bmal 1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)。
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          RDB14177 pGL3_hBmalPro-Luc (-829_+57) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Circadian clock (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14177_A6F3p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Region: insert 5'
          Sequence file: RDB14177_A6F3a.seq check
          >D03420A9_A6F3_1_pGL4-4174F_F03_18.ab1
              1 NNNNNNNNNN NNTNNNNNNT CGNNAGGTNN CGNNCTCTTA CGCGTGCTAG CCCGGGCTCG
             61 AGTATTCTCT TTGGAACTAC GGGAATGACA GCTCCAGTGG GACACTTGGA GGTCATGATG
            121 AACGCAAAGA ACGTGAGAAC ACTTGCAGTC CTCGGGGTGG AAATGCCTTC TAGAAATACT
            181 AAGTATCCGT TCTCTCGTTG AGACTTTGGC AAACCAGGGA TTTTAGGAAG GGTCTGGCAC
            241 TCAACGTTCC TAAAATTGGT TTCCTAGATG GAGCCGGAGG GAGAGAGGGA GTCAGGAACT
            301 GCTGCTTTTC GTCGGAATGC CCGGAGGGGA GAAGAAGAAG GAGGAGGGGT GAGGGCGGGC
            361 GGCCAAACGC CAGCCGGCAG GGGACCCAGA GAAGAGGGAC ATCCCGGGCG ACCCCGAGGA
            421 GCGCGGCTTG GGCACCGCAG TGGCCGCGGC TAGTGGGAGA CCTGAGGGGA AAGGGAGAGG
            481 GCAGGGGCGA GGAACCCAGG GAGCGCGCGC GGATTGGTCC TCTCCTCGGG GCGTGCGCTC
            541 CTGTGCGCCA AATGATTGGT GGCAGGAAAG TAGCAGGTAA ACCGGCTCCC GCCGCCTCGC
            601 CATTGGTCAG CGGCCTCTCT AGCCGCCACA CAACTGGCCA GCGGGCTGCC GAGCCGCGCG
            661 CGGATTGGCT GGNGGCGGCC GCCGGGACCG GCTCCCTTCG GGCGTTCGGA TTGGCTGGCG
            721 GGAAGANGCA NGTATCCGGG CGCTGCGGCT CCTCCATTGG TGGGCGGGGA ANGGGGGGGN
            781 NNGGCNCAGC GANTGNNGGG GCGGGGGGGN NNGGGCCNGG GCCGGCGGGG NNCGGNNNNG
            841 TCGGAAAGTA NGTTANNGGT GCGACATTNN NGGAANGNAG AANNNNNNTC NNNNNNNGNA
          901 NNNNNCNGTT TACCNNNNNN NGNNNNNNNN NN
          //
          Primer: Luc_R
          Region: Luc 5',insert 3'
          Sequence file: RDB14177_A6F3b.seq check
          >D03420A9_A6F3_1_Luc_R_G03_21.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNCTCTTNNT AGCCTTATGC AGTTGCTCTC CNGCGGTTCC
             61 NTCTTCCNGC GGATAGAATG GCGCCGGGCC TTTCTTTATG TTTTTGGCGT CTTCCATGGT
            121 GGCTTTACCA ACAGTACCGG AATGCCAAGC TTACTTAGAT CGCAGATCCC GCGGCGGCGG
            181 CGGCGGTGAG TCCGGCGCGG GTAAACAGGC ACCTCCGTCC CTGACCTACT TTCTGCCTTC
            241 CCTAAATGTC GCACCACTAA CCTACTTTCC GACCAATCCG CTCCCCGCCG GCCCAGGCCC
            301 GGCCCCCCGC CCACCAATCG CTGTGCCCAA CCCCCCTTCC CCGCCCACCA ATGGAGGAGC
            361 CGCAGCGCCC GGATACCTGC CTCTTCCCGC CAGCCAATCC GAACGCCCGA AGGGAGCCGG
            421 TCCCGGCGGC CGCCCCCAGC CAATCCGCGC GCGGCTCGGC AGCCCGCTGG CCAGTTGTGT
            481 GGCGGCTAGA GAGGCCGCTG ACCAATGGCG AGGCGGCGGG AGCCGGTTTA CCTGCTACTT
            541 TCCTGCCACC AATCATTTGG CGCACAGGAG CGCACGCCCC GAGGAGAGGA NCAATCCGCG
            601 CGCGCTCCCT GGGTTCCTCG CCCCTGCCCT CTCCCTTTCC CCTCANGTCT CCCACTAGCC
            661 GCGGCCACTG CGGTGCCCAA GCCGCGCTCC TCGGGGTCGC CCGGGNNGTC CCTCTTCTCT
            721 GGGTCCCCTG CCGGCNGGCG TTNGGCCGCC CGCCCTCACN CCTCCTCCTT CTTCTTCTCC
            781 CCTCCGGGCA TTNCGACGAA AAGCAGCAGN NCCNNNNTCC CTCTCTCCCN CCNGNNNCAT
            841 CTNNGAAANC AATTTTAGGA NNGTNNNNNG CCANNNCCNN NCNNAAANNC CNNGGNTNNN
          901 NNAANNNNNN NNNN
          //
          Primer: SV40pA_R
          Region: SV40pA,Luc 3'
          Sequence file: RDB14177_A6F3c.seq check
          >14177_14177_B0J6F_SV40pA_R_H05_23_ABI24.ab1
              1 NNNNNNNNNN TCNNNNATTT NNNNAATAAA GCATTTTTTT NNCTGCATTC TAGTTGTGGT
             61 TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGCTCGA AGCGGCCGGC CGCCCCGACT
            121 CTAGAATTAC ACGGCGATCT TTCCGCCCTT CTTGGCCTTT ATGAGGATCT CTCTGATTTT
            181 TCTTGCGTCG AGTTTTCCGG TAAGACCTTT CGGTACTTCG TCCACAAACA CAACTCCTCC
            241 GCGCAACTTT TTCGCGGTTG TTACTTGACT GGCGACGTAA TCCACGATCT CTTTTTCCGT
            301 CATCGTCTTT CCGTGCTCCA AAACAACAAC GGCGGCGGGA AGTTCACCGG CGTCATCGTC
            361 GGGAAGACCT GCGACACCTG CGTCGAAGAT GTTGGGGTGT TGGAGCAAGA TGGATTCCAA
            421 TTCAGCGGGA GCCACCTGAT AGCCTTTGTA CTTAATCAGA GACTTCAGGC GGTCAACGAT
            481 GAAGAAGTGT TCGTCTTCGT CCCAGTAAGC TATGTCTCCA GAATGTAGCC ATCCATCCTT
            541 GTCAATCAAG GCGTTGGTCG CTTCCGGATT GTTTACATAA CCGGACATAA TCATAGGACC
            601 TCTCACACAC AGTTCGCCTC TTTGATTAAC GCCCAGCGTT TTCCCGGTAT CCAGATCCAC
            661 AACCTTCGCT TCAAAAAATG GAACAACTTT ACCGACCGCG CCCGGTTTAT CATCCCCCTC
            721 GGGTGTAATC AGAATAGCTG ANGTAGTCTC AGTGAGCCCA TATCCTTGCC TGATACCTGN
            781 CAGANGGAAC CTCTTGGCAA CCGCTTCCCC GACTTCCTTA GAGAGGGGAG CGCCACCAGA
            841 AGCAATTTCG TGTAAATTAG ATAAATCGTA TTTGTCNATC ANANNGCTTT TGGCGAANAA
            901 GGANAATAGG GTTGGNACCA GCAGCGNACT TNGAANCTTG NAANCCNGNA NNNNTNNNCN
          961 NNAACAGCNN NNCTTCNAAT NNNTACNTTN NNANNNNNCN NANNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl