RDB14101 - pCAGIPuro-HAmcMyc

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pCAGIPuro-HAmcMyc_DN143

Expression vector of HA tagged mouse cMyc, deletion mutant.

Catalog number	RDB14101
Resource name	pCAGIPuro-HAmcMyc_DN143
Clone info.	Expression vector of HA tagged mouse cMyc, deletion of N143 (a.a from the 1st to the 143rd of the N-terminal).
Comment	Expression was confirmed by the depositor. SV40 ori, IRES-puromycin resistance gene casette and polyoma ori and trancated largeT minigene LT20.
Vector backbone	pCAG-IP (Plasmid)
Size of vector backbone	7.2 kb
Selectable markers	Amp^r (E. coli), Pur^r (mammalian cells).
Gene/insert name	mouse Myc cDNA
Depositor\|Developer	Okazaki, Yasushi \| Iseki, Hiroyoshi \|

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mouse Myc

External Database
mouse Myc

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
Additional terms and conditions:
CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.

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提供案内 (日本国内) [open/close]

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015) Additional terms and conditions: CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。付加的提供条件：利用者は、pCAG vector に挿入されている遺伝子群を使用・公表する場合は、下記文章の掲載を必要とする ( CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. )。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

提供条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。
付加的提供条件：
利用者は、pCAG vector に挿入されている遺伝子群を使用・公表する場合は、下記文章の掲載を必要とする ( CAG Promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J. )。

提供依頼

手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
提供依頼書 [Word

]
提供同意書 (MTA、非営利機関による非営利学術研究用)[Word

]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14101	pCAGIPuro-HAmcMyc_DN143	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCAGIPuro-HAmcMyc_DN143 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14101).

Reference section:

Iseki, H., Nakachi, Y., Hishida, T., Yamashita-Sugahara, Y., Hirasaki, M., Ueda, A., Tanimoto, Y., Iijima, S., Sugiyama, F., Yagami, K., Takahashi, S., Okuda, A., Okazaki, Y., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

Further references such as user reports and related articles (go to bottom)

References and tips

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14101_A6Dcp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB14101_A6Dca.seq
>D03255J2_A6Dc_pAxCA_F1_B07_04_ABI24.ab1 1 GGTACGGTCC TGCTACATGT TCATGCCTTC TTCTTTTTCC TACAGCTCCT GGGCAACGTG 61 CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGAATTCC TCGAGTAGGC CACCATGTAC 121 CCATACGACG TCCCAGACTA CGCTAAGCTG GTCTCGGAGA AGCTGGCCTC CTACCAGGCT 181 GCGCGCAAAG ACAGCACCAG CCTGAGCCCC GCCCGCGGGC ACAGCGTCTG CTCCACCTCC 241 AGCCTGTACC TGCAGGACCT CACCGCCGCC GCGTCCGAGT GCATTGACCC CTCAGTGGTC 301 TTTCCCTACC CGCTCAACGA CAGCAGCTCG CCCAAATCCT GTACCTCGTC CGATTCCACG 361 GCCTTCTCTC CTTCCTCGGA CTCGCTGCTG TCCTCCGAGT CCTCCCCACG GGCCAGCCCT 421 GAGCCCCTAG TGCTGCATGA GGAGACACCG CCCACCACCA GCAGCGACTC TGAAGAAGAG 481 CAAGAAGATG AGGAAGAAAT TGATGTGGTG TCTGTGGAGA AGAGGCAAAC CCCTGCCAAG 541 AGGTCGGAGT CGGGCTCATC TCCATCCCGA GGCCACAGCA AACCTCCGCA CAGCCCACTG 601 GTCCTCAAGA GGTGCCACGT CTCCACTCAC CAGCACAACT ACGCCGCACC CCCCTCCACA 661 AGAAGGACTA TCCAGCTGCC AAGAGGGCCA AGTTGGACAG TGGCAGGTCC TGAAGCAGAT 721 CAGCAACAAC CGCAAGTGCT CAGCCCCCAG TCCTCAGACA CGGAGGAAAA CGACAGAGGC 781 GGACACACAA CGTCTTGGAA CGTCAGAGAG ACGAGCTGAG CGCAGCTTTT TGCCCTGGCG 841 GTGACAGAAT CCTGATGGAA ACAACGAAAG CTCCGAGGTA GTGATCCTCA AAAGGCCACC 901 GCTTACATTC TGTTCCATTC AGCGAGACGA GATAAAGCTC CACCTTGCAG ACTTATTGGA 961 CGCAGGACAT GGAACTAATC GACGCTGAGC TTGTCTTAAG TAAAG //
Primer: Myc-M_F Sequence file: RDB14101_A6Dcb.seq
>D03255J2_A6Dc_EcoRI_Myc-M_F_A04_01_ABI08.ab1 1 ACTGGCAATC CTGTACCTCG TCCGATTCCA CGGCCTTCTC TCCTTCCTCG GACTCGCTGC 61 TGTCCTCCGA GTCCTCCCCA CGGGCCAGCC CTGAGCCCCT AGTGCTGCAT GAGGAGACAC 121 CGCCCACCAC CAGCAGCGAC TCTGAAGAAG AGCAAGAAGA TGAGGAAGAA ATTGATGTGG 181 TGTCTGTGGA GAAGAGGCAA ACCCCTGCCA AGAGGTCGGA GTCGGGCTCA TCTCCATCCC 241 GAGGCCACAG CAAACCTCCG CACAGCCCAC TGGTCCTCAA GAGGTGCCAC GTCTCCACTC 301 ACCAGCACAA CTACGCCGCA CCCCCCTCCA CAAGGAAGGA CTATCCAGCT GCCAAGAGGG 361 CCAAGTTGGA CAGTGGCAGG GTCCTGAAGC AGATCAGCAA CAACCGCAAG TGCTCCAGCC 421 CCAGGTCCTC AGACACGGAG GAAAACGACA AGAGGCGGAC ACACAACGTC TTGGAACGTC 481 AGAGGAGGAA CGAGCTGAAG CGCAGCTTTT TTGCCCTGCG TGACCAGATC CCTGAATTGG 541 AAAACAACGA AAAGGCCCCC AAGGTAGTGA TCCTCAAAAA AGCCACCGCC TACATCCTGT 601 CCATTCAAGC AGACGAGCAC AAGCTCACCT CTGAAAAGGA CTTATTGAGG AAACGACGAG 661 AACAATTGAA ACACAAACTC GAACAGCTTC GAAACTCTGG TGCATAAGGT GAAGGGGGCG 721 GCCGCTCGAT AAGCTTGATA ATCGAATTGA //
Primer: IRES-F Sequence file: RDB14101_A6Dcc.seq
>D03255J2_A6Dc_IRES-F_D07_10_ABI24.ab1 1 GGCAATCTTT ATGTGTTTAG TCGAGGTTAA AAAACGTCTA GGCCCCCCGA ACCACGGGGA 61 CGTGGTTTTC CTTTGAAAAA CACGATGATA ATATGGCCAC AACCATGACC GAGTACAAGC 121 CCACGGTGCG CCTCGCCACC CGCGACGACG TCCCCAGGGC CGTACGCACC CTCGCCGCCG 181 CGTTCGCCGA CTACCCCGCC ACGCGCCACA CCGTCGATCC GGACCGCCAC ATCGAGCGGG 241 TCACCGAGCT GCAAGAACTC TTCCTCACGC GCGTCGGGCT CGACATCGGC AAGGTGTGGG 301 TCGCGGACGA CGGCGCCGCG GTGGCGGTCT GGACCACGCC GGAGAGCGTC GAAGCGGGGG 361 CGGTGTTCGC CGAGATCGGC CCGCGCATGG CCGAGTTGAG CGGTTCCCGG CTGGCCGCGC 421 AGCAACAGAT GGAAGGCCTC CTGGCGCCGC ACCGGCCCAA GGAGCCCGCG TGGTTCCTGG 481 CCACCGTCGG CGTCTCGCCC GACCACCAGG GCAAGGGTCT GGGCAGCGCC GTCGTGCTCC 541 CCGGAGTGGA GGCGGCCGAG CGCGCCGGGG TGCCCGCCTT CCTGGAGACC TCCGCGCCCC 601 GCAACCTCCC CTTCTACGAG CGGCTCGGCT TCACCGTCAC CGCCGACGTC GAGGTGCCCG 661 AAGGACCGCG CCACCTGGTG CATGACCCCG CAAGCCCGGT GCCTGACGCC CGCCCCCACG 721 ACCCGCAGCG CCCGACCGAA GGAGCGCCAC GACCCATGCA TCGATGATCT AGAGCTCCGC 781 TGATCAGCCT CGACTGGTGC CTTCTAGTGC AGCATCTGTT GTTTGCCCCT CCCCCGTGCT 841 CTGGACCTTG AGGTGCCACT CCCACTGTCG TTTCTAATAA ATGAAGAATT GCCACTCGCA 901 ATTGTCTGGA TAGGTCCATT CTATTCGGGG TGAGTTGCAG ACCCCAGGGA ATGAACAACT 961 CAGCATGCTG ATATTCGAGT GTG //
Primer: BGHpA_F Sequence file: RDB14101_A6Dcd.seq
>D03255J2_A6Dc_BGHpA_F_E07_13_ABI24.ab1 1 TCGTAATGGT ATTCTATTCT GGGGGGTGGG GTGGGGCAGG ACAGCAAGGG GGAGGATTGG 61 GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA TGGCTTCTGA GGCGGAAAGA 121 ACCTGCAGCC CAAGCTTGGC GTAATCATGG TCATAGCTGT TTCCTGTGTG AAATTGTTAT 181 CCGCTCACAA TTCCACACAA CATACGAGCC GGAAGCATAA AGTGTAAAGC CTGGGGTGCC 241 TAATGAGTGA GCTAACTCAC ATTAATTGCG TTGCGCTCAC TGCCCGCTTT CCAGTCGGGA 301 AACCTGTCGT GCCAGCGGAT CCGCATCTCA ATTAGTCAGC AACCATAGTC CCGCCCCTAA 361 CTCCGCCCAT CCCGCCCCTA ACTCCGCCCA GTTCCGCCCA TTCTCCGCCC CATGGCTGAC 421 TAATTTTTTT TATTTATGCA GAGGCCGAGG CCGCCTCGGC CTCTGAGCTA TTCCAGAAGT 481 AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTAACTTGT TTATTGCAGC 541 TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC 601 ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG TCTGGATCCG 661 CTGCATTAAT GAATCGGCCA ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC 721 GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT 781 CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG 841 TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC 901 CATAGGCTCC GCCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 961 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 1021 TCCTGTTCCG ACCCTGCCGC TTACGGGATA CCCTGTCCGC CTTTCTCCCG TCCGGAGCGT 1081 GCGCTTTCTC ATAGCTCACG CTGTAAGTAT CTCAGTCGAT GTAAGGTCGG TTCGTTCAGC 1141 TGACATGATG ATGACACGCG GA //
Primer: Amp_R Sequence file: RDB14101_A6Dce.seq
>D03255J2_A6Dc_Amp_R_F07_16_ABI24.ab1 1 CTCGTTTCAT GACCGCTTTG GAAAACAAAG ACTGTATTTC CTGGAAATTA ATGTTTATTC 61 AATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG 121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC 181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC 241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG 301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG 361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT 421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT 481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA 541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG 601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT 721 GATATCATCG TCGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT 781 TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG 841 GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA 901 CGCCCAATAG GGACTTTCCC ATTGACGTCA ATGGTGGAGT ATTTACGGTA AACTGCCCAC 961 TTGGCAGTAC ATCAGTGTAT CATATGCCAA GTACGCCCCC TATGACGTCA TGACGTAATT 1021 GACCGCTGCC ATATGCCAGT ACATGACGTA ATGGAACCTT ACTACTGGCA GTACCTTACG 1081 TATAGTTCAA TCGCCTATTA C //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.
user_report	Mise, Y., Immunosuppressive tumor microenvironment in Uterine Serous Carcinoma via CCL7 signal with myeloid-derived suppressor cells. Carcinogenesis bgac032 (2022). PMID 35353883.

2023.05.22

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