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pMXs-mEzh2

Retroviral expression vector of mouse Ezh2, variant 1.

Catalog number RDB14058
Resource name pMXs-mEzh2
Clone info. Retroviral expression vector of mouse Ezh2, variant 1.
Comment Expression was confirmed by the depositor.
Vector backbone pMXs Retroviral Vector (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name mouse Ezh2 cDNA
Depositor|Developer Okazaki, Yasushi | Iseki, Hiroyoshi |
Other clones in our bank

External Database
mouse Ezh2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)
          Additional terms and conditions for distribution Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Iseki, H. et al., Stem Cell 34(2):322-333, 2015)。
          付加的提供条件 pMXsベクターについて:利用者は、研究成果の公表にあたって pMXsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄先生への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14058 pMXs-mEzh2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14058_A6Dcp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMLV-pol_F2
          Sequence file: RDB14058_A6Dca.seq check
          >D03255E4_A6Dc_2_MMLV-pol_F2_F07_16.ab1
              1 TAGGGGGGGT GGAAATCCTC TAGACTGCCG GATCTAGCTA GTTAATTAAG GATCCACCAT
             61 GGGCCAGACT GGGAAGAAAT CTGAGAAGGG ACCGGTTTGT TGGCGGAAGC GTGTAAAATC
            121 AGAGTACATG AGACTGAGAC AGCTCAAGAG GTTCAGAAGA GCTGATGAAG TAAAGACTAT
            181 GTTTAGTTCC AATCGTCAGA AAATTTTGGA AAGAACTGAA ACCTTAAACC AAGAGTGGAA
            241 GCAGCGGAGG ATACAGCCTG TGCACATCAT GACTTCTGTG AGCTCATTGC GCGGGACTAG
            301 GGAGTGTTCA GTCACCAGTG ACTTGGATTT TCCAGCACAA GTCATCCCGT TAAAGACCCT
            361 GAATGCAGTC GCCTCGGTGC CTATAATGTA CTCTTGGTCG CCCTTACAAC AGAATTTTAT
            421 GGTGGAAGAC GAAACTGTTT TACATAACAT TCCTTATATG GGGGATGAAG TTCTGGATCA
            481 GGATGGCACT TTCATTGAAG AACTAATAAA AAATTATGAT GGAAAAGTGC ATGGTGACAG
            541 AGAATGTGGA TTTATAAATG ATGAAATTTT TGTGGAGTTG GTAAATGCTC TTGGTCAATA
            601 TAATGATGAT GATGATGACG ATGATGGAGA TGATCCAGAT GAAAGAGAAG AAAAACAGAA
            661 AGATCTAGAG GATAATCGAG ATGATAAAGA AACTTGCCCA CCTCGGAAAT TTCCTGCTGA
            721 TAAAATATTT GAAGCCATTT CCTCAATGTT TCCAGATAAG GGCACCGCAG AAGAACTGAA
            781 AGAAAAATAT AAAGAACTCA CGGAGCAGCA GCTCCCAGGT GCTCTGCCTC CTGAATGTAC
            841 TCCAAACATC GATGGACCAA ATGCCAAATC TGTTCAGAGG GAGCAAAGCT TGCATTCATT
            901 TCATACGCTC TTCTGTCGAC GATGTTTTAA GTATGACTGC TTCCTACATC CCTTCCATGC
            961 AACACCCAAC ACATATAAGA GGAAGAACAC AGAAACAGCT TTGGACAACA AAGCCCTTTG
           1021 TGGACCACAG TGTTTACCAG CATCTGGGAG GGAGCTAAAG AGTTTGCTGC TGCTCTTACT
           1081 GCTGAGCGTA ATAAAAGAAC ACAACTTAAC GGCCAGTGCG CAGAGGAGGA AAGGACTTAC
          1141 GAATTACG
          //
          Primer: pMXs-3LTRup_R2
          Sequence file: RDB14058_A6Dcb.seq check
          >D03255E4_A6Dc_2_pMXs-3LTRup_R2_G08_20.ab1
              1 CTAATAAATC TTTTATTTTA TCGCGAGTCA AGGGATTTCC ATTTCTCGTT CGATGCCCAC
             61 ATACTTCAGG GCATCAGCCT GGCTGTATCT GTAATCAAAA AACAACTCTT CACCAGTCTG
            121 GATAGCCCTC TTAGCAAAGA TGCCTATCCT GTGGTCACCA TTAACCATCA TAACTTTTGC
            181 ATAGCAGTTT GGATTTACTG AATGATTAGC AAAACGAATT TTGTTGCCCT TTCGGGTTGC
            241 ATCCACCACA AAATCATTGT TCAAGTTGAA CAGAAAGCTG CACATGTATT TGTCATACAC
            301 TTTTCCTCTT CTGTCTGCTT CATCCTGAGA AATAATCTCC CCACAGTATT CTGAGATGAA
            361 TTCATTTTTC TGTACAGGAT CTTTGATAAA GATGCCCCAG CCTGCCACAT CAGACGGTGC
            421 CAGCAGTAAG TGCTTTTTAG AGCCCCGCTG AATGCTACAG TTCTTACAGG ATACATTTTT
            481 ACTGTCCCAA TGGTCAGCAG CTCCACACGT GAGACAGAGG TCAGGGTCAC ACTCTCGGAC
            541 AGCCAGGTAG CATGGACACT GTTTGGTGTT GCATTGTGCT TTGCACCGAC ATCCAGGAAA
            601 GCGGTTTTGA CCCTTTTTCA GTTGTATCTT TCTGCAGTGT GCAGCCCACA ACCGATGTTT
            661 CCTTTTCTTC TTTCTTGGAG GAGTGTCTAC ATCCTCAGTG GGAACAGGTG CTATGATACT
            721 GGACTCCTTG ACTCTAAACT CATACACCTG TCTACATGTT TTGGTCCCAA TTAGCCTAGC
            781 AATGGCACAA AAGTTATCGT AGTAAGTACC AATGAGGACT CTAAACATGG AGGCTTCAGC
            841 ACCACTCCAC TCCACATTCT CAGGAGGTTC AATATTTGGC TTCATCTTTA TTGGTGTTTG
            901 ACACCGAGAA TTTGCTTCAG AGGAGCTGGA CGTCTCATCT TTTTTTCTCT TCTTCTTCTT
            961 TATCATTGTT CTCTCCCCCC AGTTTCAGTC CCTGCTTCTC TGTCACTGTC TGTATCCCTT
           1021 TGACTCCAGC ACACTGATGG TTGGGGTGCT GGGGTCTGCT ACTGGTTATT CGAAAGTCTT
          1081 CCTCATCCTG CCGACCCCCT TGCGCCGATT TAAGGTGGTT GATCCTTTTA AATAAAGCG
          //
          Primer: RDB14058#1
          Sequence file: RDB14058_A6Dcc.seq check
          >D03255E4-2_A6Dc_RDB14058#1_D01_04_ABI08.ab1
              1 GGGGCAGAGT TTTAGTATGA CTGCTTCCTA CATCCCTTCC ATGCAACACC CAACACATAT
             61 AAGAGGAAGA ACACAGAAAC AGCTTTGGAC AACAAGCCTT GTGGACCACA GTGTTACCAG
            121 CATCTGGAGG GAGCTAAGGA GTTTGCTGCT GCTCTTACTG CTGAGCGTAT AAAGACACCA
            181 CCTAAACGCC CAGGGGGCCG CAGAAGAGGA AGACTTCCGA ATAACAGTAG CAGACCCAGC
            241 ACCCCCACCA TCAGTGTGCT GGAGTCAAAG GATACAGACA GTGACAGAGA AGCAGGGACT
            301 GAAACTGGGG GAGAGAACAA TGATAAAGAA GAAGAAGAGA AAAAAGATGA GACGTCCAGC
            361 TCCTCTGAAG CAAATTCTCG GTGTCAAACA CCAATAAAGA TGAAGCCAAA TATTGAACCT
            421 CCTGAGAATG TGGAGTGGAG TGGTGCTGAA GCCTCCATGT TTAGAGTCCT CATTGGTACT
            481 TACTACGATA ACTTTTGTGC CATTGCTAGG CTAATTGGGA CCAAAACATG TAGACAGGTG
            541 TATGAGTTTA GAGTCAAGGA GTCCAGTATC ATAGCACCTG TTCCCACTGA GGATGTAGAC
            601 ACTCCTCCAA GAAAGAAGAA AAGGAAACAT CGGTTGTGGG CTGCACACTG CAGAAAGATA
            661 CAACTGAAAA AGGGTCAAAA CCGCTTTCCT GGATGTCGGT GCAAAGCACA ATGCAACACC
            721 AAACAGTGTC CATGCTACCT GGCTGTCCGA GAGTGTGACC CTGACCTCTG TCTCACGTGT
            781 GGAGCTGCTG ACCATTGGGA CAGTAAAAAT GTATCCTGTA AGAACTGTAG CATTCAGCGG
            841 GGCTCTAAAA AGCACTTACT GCTGGCACCG TCTGATGTGG CAGGCTGGGG CATCTTTATC
            901 AAAGATCCTG TACAGAAAAA TGAATTCATC TCAGAATACT GTGGGGAGAT TATTTCTCAG
            961 GATGAAGCAG ACAGAAGAGG AAAAGTGTAT GACAAATACA TGTGCAGCTT TCTGTTCAAC
           1021 TTGAACAATG ATTTTGGTGG TGGATGCAAC CCGAAGGCAC AAATTCGGTT TTGCTTAATC
           1081 ATTCAGTTAA ATCCAAACTG CTATGGCAAA GGTAATGGAA TGGATAAATG GGTTGACCCA
          1141 CAGGAATAGG GCAATCTCTT TTGTTC
          //
          Primer: Amp_R
          Sequence file: RDB14058_A6Dcd.seq check
          >D03255E4-2_A6Dc_Amp_R_A01_01_ABI08.ab1
              1 TTTATTTTTC ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
             61 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
            121 CACCTGCATA ATGAAAGACC CCACCTGTAG GTTTGGCAAG CTAGCTTAAG TAACGCCATT
            181 TTGCAAGGCA TGGAAAAATA CATAACTGAG AATAGAAAAG TTCAGATCAA GGTCAGGAAC
            241 AGATGGAACA GCTGAATATG GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG
            301 CTCAGGGCCA AGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT CTGTGGTAAG
            361 CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGTCCC CAGATGCGGT CCAGCCCTCA
            421 GCAGTTTCTA GAGAACCATC AGATGTTTCC AGGGTGCCCC AAGGACCTGA AATGACCCTG
            481 TGCCTTATTT GAACTAACCA ATCAGTTCGC TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC
            541 CCGAGCTCAA TAAAAGAGCC CACAACCCCT CACTCGGCGC GCCAGTCCTC CGATTGACTG
            601 AGTCGCCCGG GTACCCGTGT ATCCAATAAA CCCTCTTGCA GTTGCATCCG ACTTGTGGTC
            661 TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG TGATTGACTA CCCGTCAGCG GGGGTCTTTC
            721 ATTTGGGGGC TCGTCCGGGA TCGGGAGACC CCTGCCCAGG GACCACCGAC CCACCACCGG
            781 GAGGTAAGCT GGCCAGCAAC TTATCTGTGT CTGTCCGATT GTCTAGTGTC TATGACTGAT
            841 TTTATGCGCC TGCGTCGGTA CTAGTTAGCT AACTAGCTCT GTATCTGGCG GACCCGTGGT
            901 GGAACTGACG AGTTCGGAAC ACCCGGCCGC AACCCTGGGA GACGTCCCAG GGACTTCGGG
            961 GGCCGTTTTT GTGGCCCGAC CTGAGTCCAA AATCCCGATC GTTTTGGACT CTTTGGTGCA
           1021 CCCCCCTAAT AGGAGGATAT GTGGGTTCTG GGTAGAGACG AGACCCTAAA CAGTTCCGCT
           1081 CGTCTGATTT TTGCTTCGGC TTGGACGAGC CGCGACGCGC CGTCCTTGTC TTGCTGCCAA
          1141 GG
          //
          Primer: pMFG_1
          Sequence file: RDB14058_A6Dce.seq check
          >D03255E4-2_A6Dc_pMFG_1_B01_02_ABI08.ab1
              1 GGATGGGGAA GGAAGGGGGC TGTTAGTAAC TCTTGTCATG TCAGGGTCAG GGAAGTTTAC
             61 AAGGGGCGGG GGTGCCCCAT ATAAGATCTC ATATGGCCAT ATGGGGGCGC CTAGAGAAGG
            121 AGTGAGGGCT GGATAAAGGG AGGATCGAGG CGGGGTCGAA CGAGGAGGTT CAAGGGGGAG
            181 AGACGGGGCG GATGGAGGAA GAGGAGGCGG AGGCTTAGGG TGTACAAAGG GCTTGACCCA
            241 GGGAGGGGGG TCAAAAGCCA AGGCTTCCCA GGTCACGATG TAGGGGACCT GGTCTGGGTG
            301 TCCATGCGGG CCAGGTGAAA AGACCTTGAT CTTAACCTGG GTGATGAGGT CTCGGTTAAA
            361 GGTGCCGTCT CGCGGCCATC CGACGTTAAA GGTTGGCCAT TCTGCAGAGC AGAAGGTAAC
            421 CCAACGTCTC TTCTTGACAT CTACCGACTG GTTGTGAGCG ATCCGCTCGA CATCTTTCCA
            481 GTGACCTAAG GTCAAACTTA AGGGAGTGGT AACAGTCTGG CCCTAATTTT CAGACAAATA
            541 CAGAAACACA GTCAGACAGA GACAACACAG AACGATGCTG CAGCAGACAA GACGCGCGGC
            601 GCGGCTTCGG TCCCAAACCG AAAGCAAAAA TTCAGACGGA GGCGGGAACT GTTTTAGGTT
            661 CTCGTCTCCT ACCAGAACCA CATATCCCTC CTATTAGGGG GGTGCACCAA AGAGTCCAAA
            721 ACGATCGGGA TTTTTGGACT CAGGTCGGGC CACAAAAACG GCCCCCGAAG TCCCTGGGAC
            781 GTCTCCCAGG GTTGCGGCCG GGTGTTCCGA ACTCGTCAGT TCCACCACGG GTCCGCCAGA
            841 TACAGAGCTA GTTAGCTAAC TAGTACCGAC GCAGGCGCAT AAAATCAGTC ATAGACACTA
            901 GACAATCGGA CAGACACAGA TAAGTTGCTG GCCAGCTTAC CTCCCGGGTG GTGGGTCGGT
            961 GGTCCCTGGG CAGGGGTCTC CCGATCCCGG GACGAGCCCC CAAAATGAAA GACCCCCGCT
           1021 GACGGGTAGT CAAATCACTC AGAAGGGAGA CCCTCCCAAG GGAACAAGCG AGGACCAACA
           1081 AGTTCGGGAT GGCAACTGCA AGAGGGTTAT GATTACACGG GTACCGGGCC GACTCAAGTC
          1141 AAATCGGGAG AAGGAACCTT GTGGCTGC
          //
          Primer: SV40pA3'Element Reverse
          Sequence file: RDB14058_A6Dcf.seq check
          >D03255E4-2_A6Dc_SV40pA3'ElementReverse_C01_03_ABI08.ab1
              1 GCGGGCCCAG GGGGGGCCGG GGGCTATGGA AACGCCAGCA ACGCGGCCTT TTTACGGTTC
             61 CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG
            121 GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG
            181 CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCCAA TACGCAAACC GCCTCTCCCC
            241 GCGCGTTGGC CGATTCATTA ATGCAACTAT GGCCATTTAA TGTAAATACT TAAGAAAAAA
            301 AACCAAATTA ATTTTGATAC ATGCTGCATG TGAAAGACCC CCGCTGACGG GTAGTCAATC
            361 ACTCAGAGGA GACCCTCCCA AGGAACAGCG AGACCACAAG TCGGATGCAA CTGCAAGAGG
            421 GTTTATTGGA TACACGGGTA CCCGGGCGAC TCAGTCAATC GGAGGACTGG CGCCCCGAGT
            481 GAGGGGTTGT GGGCTCTTTT ATTGAGCTCG GGGAGCAGAA GCGCGCGAAC AGAAGCGAGA
            541 AGCGAACTGA TTGGTTAGTT CAAATAAGGC ACAGGGTCAT TTCAGGTCCT TGGGGCACCC
            601 TGGAAACATC TGATGGTTCT CTAGAAACTG CTGAGGGCTG GACCGCATCT GGGGACCATC
            661 TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT TTGGCCCATA
            721 TTCAGCTGTT CCATCTGTTC TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT
            781 CCTGTTTGGC CCATATTCAG CTGTTCCATC TGTTCCTGAC CTTGATCTGA ACTTCTCTAT
            841 TCTCAGTTAT GTATTTTTCC ATGCCTTGCA AAATGGCGTT ACTTAAGCTA GCTTGCCAAA
            901 CCTACAGGTG GGGTCTTTCA TTCCCCCCCT TTTTTCTGGA GACTAAATAA AATCTTTTAT
            961 TTTATCGCGA GTCAAGGGGA ATTTCCATTT CTCGTTCGAT GCCCACATAC TTTCAAGGGC
           1021 ATCAGCCCTG GCTGTATCTG TAATCAAAAA ACAACTCTTC CACCAGTCTG GGATAGTCCT
           1081 TCTTTAGCAA AGATGTCTAA TCTTGTTGTT CACCATTAGC CCATCCATAA CCGTTTTGGC
          1141 ATATAGG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Iseki, H., Combined Overexpression of JARID2, PRDM14, ESRRB, and SALL4A Dramatically Improves Efficiency and Kinetics of Reprogramming to Induced Pluripotent Stem Cells. Stem Cells 34 (2): 322-333 (2016). PMID 26523946.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl