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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-HERC2

Expression vector of human HERC2.

Catalog number RDB13974
Resource name pEF-HERC2
Clone info. Expression vector of human HERC2.
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone 5.3 kb
Selectable markers Amp^r
Gene/insert name human HERC2 cDNA
Depositor|Developer Imai, Yuzuru |
Other clones in our bank
External Database
human HERC2
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)
          Additional terms and conditions:
          Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Imai, Y., PLoS Genet. 11(9): e1005503 2015.)。
          付加的提供条件：
          pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB13974 pEF-HERC2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEF-HERC2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13974).

          Reference section:

          Imai, Y., Kobayashi, Y., Inoshita, T., Meng, H., Arano, T., Uemura, K., Asano, T., Yoshimi, K., Zhang, C.L., Matsumoto, G., Ohtsuka, T., Kageyama, R., Kiyonari, H., Shioi, G., Nukina, N., Hattori, N., Takahashi, R., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13974_A6C2p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB13974_A6C2a.seq check
          >D03172A2_A6C2_1_EF1a_C_F_E11_14.ab1
              1 TATGGGATTG CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
             61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGTCGAGAT GCCCTCTGAA
            121 TCTTTCTGTT TGGCTGCCCA GGCTCGCCTC GACTCCAAAT GGTTGAAAAC AGATATACAG
            181 CTTGCATTCA CAAGAGATGG GCTCTGTGGT CTGTGGAATG AAATGGTTAA AGATGGAGAA
            241 ATTGTATACA CTGGAACAGA ATCAACCCAG AACGGAGAGC TCCCTCCTAG AAAAGATGAT
            301 AGTGTCGAAC CAAGTGGAAC AAAGAAAGAA GATCTGAATG ACAAAGAGAA AAAAGATGAA
            361 GAAGAAACTC CTGCACCTAT ATATAGGGCC AAGTCAATTC TGGACAGCTG GGTATGGGGC
            421 AAGCAACCAG ATGTGAATGA ACTGAAGGAG TGTCTTTCTG TGCTGGTTAA AGAGCAGCAG
            481 GCCCTGGCCG TCCAGTCAGC CACCACCACC CTCTCAGCCC TGCGACTCAA GCAGAGGCTG
            541 GTGATCTTGG AGCGCTATTT CATTGCCTTG AATAGAACCG TTTTTCAGGA GAATGTCAAA
            601 GTTAAGTGGA AAAGCAGCGG TATTTCTCTG CCTCCTGTGG ACAAAAAAAG TTCCCGGCCT
            661 GCGGGCAAAG GTGTGGAGGG GCTCGCCAGA GTGGGATCCC GAGCGGCGCT GTCTTTTGCC
            721 TTTGCCTTCC TGCGCAGGGC CTGGCGATCA GGCGAGGATG CGGACCTCTG CAGTGAGCTG
            781 TTGCAGGAGT CCCTGGACGC CCTGCGAGCA CTTCCCGAGG CCTCGCTCTT TGACGAGAGC
            841 ACCGTGTCCT CTGTGTGGCT GGAGGTGGTG GAGAGAGCGA CCAGGTTTCC TCAGGTCCGT
            901 CGTGACGGGG GGATGTTCAC GGAACGCCAG CCACCAAAGG GCCAGGAAGC ATCCCCCCTG
            961 CAGACCAGCA CTTGGCCCTG GCCCATCCTT GCTGGGAGCT GGCTGTGCAG AGGAGGCACG
           1021 CTTGAGCCCA AATGTTTGTC TGCCCATTCC TTGTTTGTTT GCTTTCAGCT GTGGGGACAG
          1081 CGGGGCCACA GGAGACCTGG ACTATGAAGC GATTCCGCCC CAGGGGCAAC CAAGT
          //
          Primer: Poly(A) region primer #0
          Sequence file: RDB13974_A6C2b.seq check
          >D03172A2_A6C2_1_GNPmixture_D01_10.ab1
              1 AGGAAAAATT CGGTGAAAAT TCAGTGTACA ATGTGACAGT GGAGGGGACA CTGGTGAGTG
             61 GGGGGTGAAG AGGTGGAGGG TGACAGTGGA GCTTCCTTTC ACACACAGGC CTGACATGAT
            121 TCCTCCTGTC TGCTCCCTCC CACATCCCCA GTCCCCGTCC AGCAAGGCAA ATGTCCAGGC
            181 ACCTGCCTGG CCCTAAAAAG AGTCACAGTC CTGACCCTCG TGGGGGCAGG GCAGGTGTCC
            241 CGAGGCCACC CAGAAAAACA GGAGACACGT CGGTGATGTT CGGGAGTCAA ACCATGTCCC
            301 AAAAGTCTTA AGAAGAAAAA CGAGATTCCC CAGCAAATTC GTGGGACCCC AGGGCCATGC
            361 CTCCCAGCTC TGGCCACCTC TGCTTGTCCA GGGGCCTCAC AGCCAAGGGG ATGCAGGGGC
            421 CGCTGTGCAG TCGGCTGAGG CCAGAGTGAG GGGTGCAAGG ACCCAGATGG GGAACACTGC
            481 TGTTTAAATA TTAAACAGGG ATTTCTTGTC TCCCACGTGG GAGACCTGAT ACTCTCAAGG
            541 GTCCCAGGTG GGGACCCTCA CTCTAGGCTT TACTTGTACA GGGCCGCTTA GTGTCCTGTT
            601 AAATAATCTT GTGTAGAGTC CGAAGCAAAG GAGTCGACAT CCTCGTTATC TGAATCGTCG
            661 CTGCTGTCGT CGGCGGCTGG CTCTCCTGTA AGTGCGATGC GAGCGTAGTC ATCTGTGTCT
            721 ATGGACTTGC AGAAGTGGAT GGCGTACTTG AGCTTCTCCT CCAGCACCTG CTTGCAGGAA
            781 TACCTGGGCA GCTTTCAGCA AGAAGAAACA GGTGTAGGAC TCAGGGAGGA AGTGGGTCTG
            841 GAGGGTTGTA TTTATCCAAA CACCTGGATG ACGAAAGTCT TCGGCCCCGG AAGTCGCCGA
            901 TGGGTCCTGG GCTACCCTCG TCCGGCCCCA GAACGAAAGC AAAAGAAAAA GAGAACGCTC
            961 TGTTGTTTGG AAAAAGGACA CCAATCACCT TCCCAAAAAT CAACTTGGAT TCAGCGGATT
           1021 GACGGAAAGG GCTTCGAATG GCCTTTTTAA TAGGTGGATC AACCCGAAAC TTTTGAAGAA
          1081 AAGTAGCTAG ACCGGA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Imai, Y., The Parkinson's Disease-Associated Protein Kinase LRRK2 Modulates Notch Signaling through the Endosomal Pathway. PLoS Genet. 11 (9): e1005503 (2015). PMID 26355680. [link to RRC of NBRP]

          2023.08.17

          GNP_filter3_RDBDEP_html_230702.pl