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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pQE30/His6-Venus-NT-Lys

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin.

Catalog number RDB13959
Resource name pQE30/His6-Venus-NT-Lys
Clone info. Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of His6-Venus-tagged non toxic lysenin.
Comment Expression was confirmed by the depositor.
Vector backbone pQE-30 (Plasmid)
Size of vector backbone 3.5 kb
Selectable markers Amp^r
Gene/insert name Eisenia foetida lysenin cDNA Aequorea victoria GFP cDNA
Depositor|Developer Kobayashi, Toshihide | Abe, Mitsuhiro |
Other clones in our bank

External Database
Eisenia foetida lysenin
    Aequorea victoria GFP

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Kiyokawa, E., J Biol Chem. 280 (25): 24072-24084, 2005.)
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            The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kiyokawa, E., J Biol Chem. 280 (25): 24072-24084, 2005.)。

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            RDB13959 pQE30/His6-Venus-NT-Lys DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pQE30/His6-Venus-NT-Lys was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13959).

            Reference section:

            Kiyokawa, E., Baba, T., Otsuka, N., Makino, A., Ohno, S., Kobayashi, T., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Visualization of Organelles (English text)
            Featured content Sphingolipid signaling pathway (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13959_A6Btp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pJB8_Forward
            Sequence file: RDB13959_A6Bta.seq check
            >D03144A2_A6Bt_1_pJB8_Forward_C12_09.ab1
                1 CTCAACTTAT TTGCTTTGTG AGCGGATAAC AATTTCACAC AGAATTCATT AAAGAGGAGA
               61 AATTAACTAT GAGAGGATCG CATCACCATC ACCATCACGG ATCCACTAGT AACGGCCGCC
              121 AGTGTGCTGG AATTCGCCCT TCCGGATCCA TGGTGAGCAA GGGCGAGGAG CTGTTCACCG
              181 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCAGCGTGT
              241 CCGGTGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC CCTGAAGCTG ATCTGCACCA
              301 CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC CCTGGGCTAC GGCATGCAGT
              361 GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG
              421 AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA CGGCAACTAC GAGACCCGCG
              481 CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT CGAGCTGAAG GGCATCGACT
              541 TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA CAACTACAAC AGCCACAACG
              601 TCTATATCAC CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGCCACA
              661 ACATCGAGGA CGGCGGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG
              721 ACGGCCCCGT GCTGCTGCCC GACAACCACT ACCTGAGCTA CCAGTCCAAA CTGAGCAAAG
              781 ACCCCAACGA GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA
              841 CTCTCGGCAT GGACGAGCTG TACAAGATAA TCCTTGGAAA AACAGAAATT CGAATTAAGC
              901 ATGCAGAAAG GAAGGAGTAC ATGACAGTCG TTTCAAGAAA AAGTTGGCCA GCTGCAACTC
              961 TTGGGACATA GCAACTTTTC AAGTTTGTGC TCTATGAAGA TTGGGGGGGG GGATTTCGAA
             1021 ATTTAAAACG CTGACACCAA TGTATTCGGG CTATGAGTAT GCCCTAATCC TCTGAATCAC
            1081 GAAGGAGTCT ACCTTGGATC AGTACTGGAT ATTCCGAAAA CAGGCCGCCT GGGGCCAAA
            //
            Primer: Cm_N_rev
            Sequence file: RDB13959_A6Btb.seq check
            >D03144A2_A6Bt_1_Cm_N_rev_D12_12.ab1
                1 CGAGTCTCAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT
               61 TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC GCCAAGCTAG CTTGGATTCT
              121 CACCAATAAA AAACGCCCGG CGGCAACCGA GCGTTCTGAA CAAATCCAGA TGGAGTTCTG
              181 AGGTCATTAC TGGATCTATC AACAGGAGTC CAAGCTCAGC TAATTAAGCT TTTAACCAAC
              241 CACTTCCAAA ATCCACTTGT CTTCACGTTT GTCCAGACAG TACACGTTTG TTGCCGGTCC
              301 ATCATCGTAG CACAGCCCAC TGCGAGTGAA GTACTTATTC ATGAAGGTGA CTACGTCACC
              361 ATGACGAAGA GGCAATGACT TATTGATTGC CCAGCGCTGT TTCGGATTAT CAGTACCCTG
              421 ATCAAAGTAG ATTCCTCCTT GATCAGAGGA ATAGGCATAC TCATAGCCCG AATACATGGT
              481 GTTCAGCGTT TTAATTCGAA ATCCCCCCCA ATCTTCATAG AGCACAAACT TGAAAAGTTT
              541 GCTATGTCCA AGAGTTGCAG CTGGCCAACT TTTTCTTGAA ACGACTGTCA TGTACTCCTT
              601 CCTTTCTGCA TGCTTAATTC GAATTTCTGT TTTTCCAAGG ATTATCTTGT ACAGCTCGTC
              661 CATGCCGAGA GTGATCCCGG CGGCGGTCAC GAACTCCAGC AGGACCATGT GATCGCGCTT
              721 CTCGTTGGGG TCTTTGCTCA GTTTGGACTG GTAGCTCAGG TAGTGGTTGT CGGGCAGCAG
              781 CACGGGGCCG TCGCCGATGG GGGTGTTCTG CTGGTAGTGG TCGGCGAGCT GCACGCCGCC
              841 GTCCTCGATG TTGTGGCGGA TCTTGAAGTT GGCCTTGATG CCGTTCTTCT GCTTGTCGGC
              901 GGTGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC
              961 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA
             1021 ACTTCACCCT CGGCGCGGGT CTCGTAGTTG CCGTCGTCTT TGAAGAAGAT GGTTGCGCTT
            1081 CTTGGACGTT AGCATTCCGG TCAATGGACG GGACTTGAAA GAAAAGATCG GATG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Kiyokawa, E., Spatial and functional heterogeneity of sphingolipid-rich membrane domains. J. Biol. Chem. 280 (25): 24072-24084 (2005). PMID 15840575. [link to RRC of NBRP]

            2023.11.23

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