A plasmid for construction of C-terminal mAID tagging donors.
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External Database Arabidopsis thaliana AtIAA17 Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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Ordering | Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
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提供依頼 | 提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13930 | pMK287 (mAID-Hygro) | DNA solution |
Materials & Methods section:
The pMK287 (mAID-Hygro) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13930). |
Reference section:
Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Auxin Inducible Degron (AID) System (English text) |
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Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13930_A6B8p1-3.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: M13_-40 (Pr0410) Region: insert 5'(linker,mAID),SV40 pA,loxP,PGK pro Sequence file: RDB13930_A6B8d.seq |
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>D03070A14_A6B8_1_M13(-40)_G03_21.ab1 1 NNNNNNNNNN NNNCNNANNN GANTNNCNAT AGGGCGAATT GGAGCTCCCC GGGNTCCGGT 61 GCAGGCGCCA AGGAGAAGAG TGCTTGTCCT AAAGATCCAG CCAAACCTCC GGCCAAGGCA 121 CAAGTTGTGG GATGGCCACC GGTGAGATCA TACCGGAAGA ACGTGATGGT TTCCTGCCAA 181 AAATCAAGCG GTGGCCCGGA GGCGGCGGCG TTCGTGAAGG TATCAATGGA CGGAGCACCG 241 TACTTGAGGA AAATCGATTT GAGGATGTAT AAATAATCTA GATAACTGAT CATAATCAGC 301 CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC 361 CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT 421 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT 481 AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTAACGCG TCGATCATAT TCAATAACCC 541 TTAATATAAC TTCGTATAAT GTATGCTATA CGAAGTTATT AGGTCTGAAG AGGAGTTTAC 601 GTCCAGCCAA GCTTAGGATC TCGACCTCGA AATTCTACCG GGTAGGGGAG GCGCTTTTCC 661 CAAGGCAGTC TGGAGCATGC GCTTTAGCAG CCCCGCTGGG CACTTGGCGC TACACAAGTG 721 GCCTCTGGCC TCGCACACAT TCCACATCCA CCGGTAGGCG CCAACCGACT CCGTTCTTTG 781 GTGGCCCCTT CGCGCCACCT TCTACTCCTC CCCTAGTCAG GAAGTTCCCC CCCGCCCCGC 841 ANCTCGCGTC GTGCNNGNNG TGACAAATGG AANTANCACG TCTCACTANT CTCGNGCANA 901 NGGACAGCAN CGCTNANCNA NGGAANCGGG NNNNNCTTNG GGGNANCNGN CNATANCANC 961 TTNNNTNNNT NNCTTTCTGG GNNCNNNNNN NNNN // |
Primer: M13R (Pr0160) Region: insert 5'(loxP,mouse PGK ter),HygR Sequence file: RDB13930_A6B8e.seq |
>D03070A14_A6B8_1_M13R_C03_09.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNAAGGGN NNNNAAGCTG GGTACCGGAT CCTAGTGAAC 61 CTCTTCGAGG GACCTAATAA CTTCGTATAG CATACATTAT ACGAAGTTAT ATTAAGGGTT 121 CCGGATCTCG ACCAGCTTCT GATGGAATTA GAACTTGGCA AAACAATACT GAGAATGAAG 181 TGTATGTGGA ACAGAGGCTG CTGATCTCGT TCTTCAGGCT ATGAAACTGA CACATTTGGA 241 AACCACAGTA CTTAGAACCA CAAAGTGGGA ATCAAGAGAA AAACAATGAT CCCACGAGAG 301 ATCTATAGAT CTATAGATCA TGAGTGGGAG GAATGAGCTG GCCCTTAATT TGGTTTTGCT 361 TGTTTAAATT ATGATATCCA ACTATGAAAC ATTATCATAA AGCAATAGTA AAGAGCCTTC 421 AGTAAAGAGC AGGCATTTAT CTAATCCCAC CCCACCCCCA CCCCCGTAGC TCCAATCCTT 481 CCATTCAAAA TGTAGGTACT CTGTTCTCAC CCTTCTTAAC AAAGTATGAC AGGAAAAACT 541 TCCATTTTAG TGGACATCTT TATTGTTTAA TAGATCATCA ATTTCTGCAT CCCGGGGATC 601 TGATATCATC GATGCATGGG GTCGTGCGCT CCTTTCGGTC GGGCGCTGCG GGTCGTGGGG 661 CGGGCGGCTA GCCTATTCCT TTGCCCTCGG ACGAGTGCTG GGGCGTCGGT TTCCACTATC 721 GGCGAGTACT TCTACACAGC CATCGGTCCN GACGGCCGCG CTTCTGCGGG CGATTTGTGT 781 ACGCCCGACA GTCCCGGCTC CGGATCGGAC GATTGCGTCN CATCGACCCT GCGCCCAANC 841 TGCATCATCG AAATTGNCGT CNACCAANCT CTGATANAGT TGGTCNAGAC NAATGCGGAN 901 CATATANNCC CNNGANCCGC NGNNATNCTG NNNGCTCCNG NANGCCTCCG CTCGAAGNNN 961 CGCGNCTGNN GNTCCNNANN ANNCNANNNN NNGGNCNTNN NNNNNNNANG NTNNGNCNNN 1021 CNNNNNNNNN GNNNNCCNNN NNNNNNN // |
Primer: PGKpro_F (Pr0249) Region: HygR Sequence file: RDB13930_A6B8f.seq |
>D03070A14_A6B8_1_PGK-proF_G07_19.ab1 1 NNNNNNNNNN NNNNNNNNNN NTCTCCNGGC CTTTCGACCT GCATCCNTCT AGATCTCGAG 61 CAGCTGAAGC TTACCGCTAG CATGGATAGA TCCGGAAAGC CTGAACTCAC CGCGACGTCT 121 GTCGAGAAGT TTCTGATCGA AAAGTTCGAC AGCGTCTCCG ACCTGATGCA GCTCTCGGAG 181 GGCGAAGAAT CTCGTGCTTT CAGCTTCGAT GTAGGAGGGC GTGGATATGT CCTGCGGGTA 241 AATAGCTGCG CCGATGGTTT CTACAAAGAT CGTTATGTTT ATCGGCACTT TGCATCGGCC 301 GCGCTCCCGA TTCCGGAAGT GCTTGACATT GGGGAATTCA GCGAGAGCCT GACCTATTGC 361 ATCTCCCGCC GTGCACAGGG TGTCACGTTG CAAGACCTGC CTGAAACCGA ACTGCCCGCT 421 GTTCTGCAGC CGGTCGCGGA GGCCATGGAT GCGATCGCTG CGGCCGATCT TAGCCAGACG 481 AGCGGGTTCG GCCCATTCGG ACCGCAAGGA ATCGGTCAAT ACACTACATG GCGTGATTTC 541 ATATGCGCGA TTGCTGATCC CCATGTGTAT CACTGGCAAA CTGTGATGGA CGACACCGTC 601 AGTGCGTCCG TCGCGCAGGC TCTCGATGAG CTGATGCTTT GGGCCGAGGA CTGCCCCGAA 661 GTCCGGCACC TCGTGCACGC GGATTTCGGC TCCAACAATG TCCTGACGGA CAATGGCCGC 721 ATAACAGCGG TCATTGACTG GAGCGAGGCG ATGTTCGGGG ATTCCCAATA CGAGGTCGCC 781 AACATCTTCT TCTGGANGCC GTGGTTGGCT TGTATGGAGC AGCAGANGCG CTACTTCGAG 841 CGGANGCATC CGGAGCTTGC ANGATCGCCG CGGCTNCNGG CGTATATGCT CCGCATTGGT 901 CTTGANCNAC TCTATCANAN CTTGGTTGAN NNNNNTTTCN ATGANGCNNC TNNNNNCNNN 961 NNNANGCNNC NNNNNNNTNC NNTNNNNANN NNNNNNGNNN GGNNNNNNNN NANNNNNNNN 1021 NN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP] |
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reference | Chen, F.X., PAF1 regulation of promoter-proximal pause release via enhancer activation. Science 357 (6357): 1294-1298 (2017). PMID 28860207. |
reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302. |
2024.03.26
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