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pGEX-NP-ncNLS

Expression vector of GST-tagged ncNLS (ASQGTKRSYEQMET).

Catalog number RDB13757
Resource name pGEX-NP-ncNLS
Clone info. Expression vector of GST-tagged influenza virus ncNLS (ASQGTKRSYEQMET).
Comment A non-classical nuclear localization signal of influenza A virus nucleoprotein. Expression was confirmed by the depositor with SDS-PAGE and crystallization. Refer Matsuura, Y. Nature 432 (7019): 872-877, 2004, PMID:15602554 for pGEX-TEV vector.
Vector backbone pGEX-TEV (Plasmid)
Selectable markers Amp^r
Gene/insert name Influenza virus nuclear protein NP cDNA
Depositor|Developer Matsuura, Yoshiyuki |
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External Database
Influenza virus nuclear protein NP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR (1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Nakada, R., Sci. Rep. 5: 15055 (2015)) (2) RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. (3) For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
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          提供条件 1.利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Nakada, R., Sci. Rep. 5: 15055 (2015)) 2.利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 3.営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。
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          RDB13757 pGEX-NP-ncNLS DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Nakada, R., Structure of importin-alpha bound to a non-classical nuclear localization signal of the influenza A virus nucleoprotein. Sci. Rep. 5: 15055 (2015). PMID 26456934.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13757_A6Acp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGEX_R
          Sequence file: RDB13757_A6Aca.seq check
          >D03041A1_A6Ac_7_pGEX_R_E05_14.ab1
              1 TTCATACGTA CTCGCGAGGC AGATCGTCAG TCAGTCACGA TGCGGCCGCT CGAGTTAAGT
             61 CTCCATTTGT TCATAAGAAC GTTTGGTGCC TTGAGACGCG GATCCCTGGA AATACAAGTT
            121 CTCCGTGGTC GGGATGTCAT AATCAGATCC ATCCGGAACC AGATCCGATT TTGGAGGATG
            181 GTCGCCACCA CCAAACGTGG CTTGCCAGCC CTGCAAAGGC CATGCTATAT ACTTGCTGGA
            241 TTTCAAGTAC TTATCAATTT GTGGGATAGC TTCAATACGT TTTTTAAAAC AAACTAATTT
            301 TGGGAACGCA TCCAGGCACA TTGGGTCCAT GTATAAAACA ACATCAAGAG CGTCATACAA
            361 CATGAAGTCA GGATGGGTTA CATGATCACC ATTTAAATAT GTTTTATGAC ATAAACGATC
            421 TTCGAACATT TTCAGCATTT CAGGTAGCTT GCTAAGAAAA TCAACTTTGA GAGTTTCAAA
            481 GTCTTTACTA TATGCAATTC TCGAAACACC GTATCTAATA TCCAAAACCG CTCCTTCAAG
            541 CATTGAAATC TCTGCACGCT CTTTTGGACA ACCACCCAAC ATGTTGTGCT TGTCAGCTAT
            601 ATAACGTATG ATGGCCATAG ACTGTGTTAA TTTAACATCA CCATCAATAT AATAAGGAAG
            661 ATTGGGAAAC TCCAAACCCA ATTCAAACTT TTTGTTTCGC CATTTATCAC CTTCATCGCG
            721 CTCATACAAA TGCTCTTCAT ATTTTTCTTC AAGATATTCC AAAAGAAGTC GAGTGGGTTG
            781 CACAAGGCCC TTAATTTTCC AATAACCTAG TATAGGGGAC ATGAATACTG TTTCCTGTGT
            841 GAAATTGTTA TCCGCTCACA ATTCCACACA TTATACGAGC CGATGATTAA TTGTCAACAG
            901 CTCATTTCAG AATATTTGCC AGAACCCGTT ATGATGTCGG CGCAAAAAAC ATTATCCAGA
            961 ACGGGAGTGC GCCTTTGAGC GACACGAATT ATGCAGTGAT TTACGACTGC ACAGCATACC
           1021 ACAGCTTTCC GATGCCTGGC TGACGCAGAA GCATTGGTGC ACGTGCAGTT CGATTAGGCT
          1081 AATTCAACGT CATGAAGATA ATTCGCGTCT GGCCTTCCTG TTAAGCC
          //
          Primer: M13(-40)
          Sequence file: RDB13757_A6Acb.seq check
          >D03041A1_A6Ac_7_M13(-40)_F05_17.ab1
              1 CCGGCAAGGA TCGTATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA
             61 ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG
            121 AGCTAACTTA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG
            181 TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC
            241 CAGGGTGGTT TTTCTTTTCA CCAGTGAGAC GGGCAACAGC TGATTGCCCT TCACCGCCTG
            301 GCCCTGAGAG AGTTGCAGCA AGCGGTCCAC GCTGGTTTGC CCCAGCAGGC GAAAATCCTG
            361 TTTGATGGTG GTTAACGGCG GGATATAACA TGAGCTGTCT TCGGTATCGT CGTATCCCAC
            421 TACCGAGATA TCCGCACCAA CGCGCAGCCC GGACTCGGTA ATGGCGCGCA TTGCGCCCAG
            481 CGCCATCTGA TCGTTGGCAA CCAGCATCGC AGTGGGAACG ATGCCCTCAT TCAGCATTTG
            541 CATGGTTTGT TGAAAACCGG ACATGGCACT CCAGTCGCCT TCCCGTTCCG CTATCGGCTG
            601 AATTTGATTG CGAGTGAGAT ATTTATGCCA GCCAGCCAGA CGCAGACGCG CCGAGACAGA
            661 ACTTAATGGG CCCGCTAACA GCGCGATTTG CTGGTGACCC AATGCGACCA GATGCTCCAC
            721 GCCCAGTCGC GTACCGTCTT CATGGGAGAA AATAATACTG TTGATGGGTG TCTGGTCAGA
            781 GACATCAAGA ATAACGCCGG AACATTAGTG CAGGCAGCTT CCACAGCAAT GGCATCCTGG
            841 TCATCCAGCG GATAGTTAAT GATCAGCCCA CTGACGCGTT GCGCGAGAAG ATTGTGGCAC
            901 CGCCGCTTTA CAGGGCTTCG AACGCCGGCT TTCGTTCTAC CAATCGAACA CAACGCTGGC
            961 ACAAGTTGGA TCCGGGCGCG GAGATTTATT CGGCTGCGAC AATTGCGACG GCCGTTGCAA
           1021 GGCCAGACTT GAGGTGGCAA CGGCAATCAG CACGACTGTT TGGCCCGCCA AGCTTGTTTG
          1081 CCACCGGTTG GGAGTATCAC GTCGGGACC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nakada, R., Structure of importin-alpha bound to a non-classical nuclear localization signal of the influenza A virus nucleoprotein. Sci. Rep. 5: 15055 (2015). PMID 26456934.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl