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pET-His UNAG

Expression clone of eel UnaG in E.coli.

Catalog number RDB13504
Resource name pET-His UNAG
Clone info. Expression clone of eel (Anguilla japonica) UnaG in E.coli.
Vector backbone pET32a (+) (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name eel UnaG cDNA
Depositor|Developer Taketani, Shigeru |
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External Database
eel UnaG

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Takeda T.A., Sci. Rep. 5, 10488 (2015)).
            Additional terms and conditions:
            RECIPIENT is requested to obtain written consent of Dr. Atsushi Miyawaki of the RIKEN CBS regarding UNAG fluorescent protein.
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            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            Approval form [Word] by Dr. Atsushi Miyawaki
            Please visit Information of Request for Distribution.[link] 
            Remarks
            Please obtain written approval of Dr. Atsushi Miyawaki.
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Takeda T.A., Sci. Rep. 5, 10488 (2015))。
            付加的提供条件:
            利用者は、UNAG蛍光タンパク質について理化学研究所CBS 宮脇敦史博士から、書面による提供承諾を得ること。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
            宮脇敦史博士による提供承諾書 [Word]
            手続きの概要は、「提供申込みについて[link]」をご覧ください。
            備考
            宮脇敦史博士の書面による提供承諾が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB13504 pET-His UNAG DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pET-His UNAG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13504).

            Reference section:

            Takeda, T.A., Mu, A., Tai, T.T., Kitajima, S., Taketani, S., Continuous de novo biosynthesis of haem and its rapid turnover to bilirubin are necessary for cytoprotection against cell damage. Sci. Rep. 5: 10488 (2015). PMID 25990790. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


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            Featured content Fluorescent protein resources (English text)
            Featured content Fluorescent protein resources (Japanese text)
            Featured content Sensor and Visualization (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13504_A7Ctp1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pET-check-F (Pr0191)
            Region: lacO,RBS,TrxA,6XHis,thrombin site,S-tag,MCS,UnaG
            Sequence file: RDB13504_A7Ctd.seq check
            >13504_13504_A7Ct_3_pET-check-F_F04_16_ABI24.ab1
                1 GGGGGTCGCA CGTCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC
               61 ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA
              121 GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG
              181 ATCGATCTCG ATCCCGCGAA ATTAATACGA CTCACTATAG GGGAATTGTG AGCGGATAAC
              241 AATTCCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGAGCGATA
              301 AAATTATTCA CCTGACTGAC GACAGTTTTG ACACGGATGT ACTCAAAGCG GACGGGGCGA
              361 TCCTCGTCGA TTTCTGGGCA GAGTGGTGCG GTCCGTGCAA AATGATCGCC CCGATTCTGG
              421 ATGAAATCGC TGACGAATAT CAGGGCAAAC TGACCGTTGC AAAACTGAAC ATCGATCAAA
              481 ACCCTGGCAC TGCGCCGAAA TATGGCATCC GTGGTATCCC GACTCTGCTG CTGTTCAAAA
              541 ACGGTGAAGT GGCGGCAACC AAAGTGGGTG CACTGTCTAA AGGTCAGTTG AAAGAGTTCC
              601 TCGACGCTAA CCTGGCCGGT TCTGGTTCTG GCCATATGCA CCATCATCAT CATCATTCTT
              661 CTGGTCTGGT GCCACGCGGT TCTGGTATGA AAGAAACCGC TGCTGCTAAA TTCGAACGCC
              721 AGCACATGGA CAGCCCAGAT CTGGGTACCG ACGACGACGA CAAGGCCATG GCTGATATCG
              781 GATCCGGTGG TTCTGGTATG GGTCGAGAAA TTTGTTGGCA CCTGGAAGAT TCGCAGACAG
            841 TCCATTAATT TTGGTTGAAT ACCC
            //
            Primer: f1ori_R3 (Pr0142)
            Region: T7 terminator,MCS,UnaG,enterokinase site,S-tag,thrombin site,6XHis,TrxA
            Sequence file: RDB13504_A7Cte.seq check
            >13504_13504_A7Ct_3_f1ori_R3_A05_02_ABI24.ab1
                1 GGCAATGGCA TCGGATTAGT TCCTCCTTTC AGCAAAAACC CCTCAAGACC CGTTTAGAGG
               61 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC
              121 GGGCTTTGTT AGCAGCCGGA TCTCAGTGGT GGTGGTGGTG GTGCTCGAGT GCGGCCGCAA
              181 GCTTGTCGAC GGAGCTCGAA TTCTCATTCC GTCGCCCTCC GGTAGCTGCG CACAGCCACG
              241 ACGTCTCCCA TCGTAAGTGT CACGACCAGT TTACCGTCCT TTATCTCTCG GACATACGTC
              301 GTCTCCTTGC CGTCCCACTT TTGTACGTAC ACCAGCTTCT CTCCCACCAA GTTCACGACA
              361 GATTTTACGC CTTTTCTTCG ATCAGAAGGA AATTCGTCGA ACTCCTCCCC TAATTTGAAC
              421 TTTACTTGAG TGTCAAGGAA CGTAGGAGGT CCATTCTCTA TTTTCACTGT CATTTTGTCT
              481 CCGTCCTTCT GGGAGATGTA CAATGTCGGC GTCGTGGCAT CCCCACCATC GCTTAATTCC
              541 TTTGGGGCTC CGATAGCTTT CAGGTATTCA CCAAAATTAT GGCTGTCTGC GATCTTCCAG
              601 GTGCCAACAA ATTTCTCGAC CATACCAGAA CCACCGGATC CGATATCAGC CATGGCCTTG
              661 TCGTCGTCGT CGGTACCCAG ATCTGGGCTG TCCATGTGCT GGCGTTCGAA TTTAGCAGCA
              721 GCGGTTTCTT TCATACCAGA ACCGCGTGGC ACCAGACCAG AAGAATGATG ATGATGATGG
              781 TGCATATGGC CAGAACCAGA ACCGGCCAGG TTAGCGTCGA GGAACTCTTT CAACTGACCT
              841 TTAGACAGTG CACCCACTTT GGTTGCCGCC ACTTCACCGT TTTTGAACAG CAGCAGAGTC
              901 GGGATACCAC GGATGCCATA TTTCGGCGCA GTGCCAGGGG TTTTGATCGA TGTTCAGTTT
              961 GCAACGGGTC AGTTTGCCCT GATATTCGTC AGCGATTTCA TCCAGAATCG GGGCGATCAT
             1021 TTTTGCACGG ACCGCACCAC CTCTGCCCAG AAATTCGAAC GAAGAATCGG CCCCCGATCC
            1081 GCATTTGAAG TACATCGATG TCCAAAACCT GATCGGTTCG AGTCAAGGGT GA
            //
            Primer: pGL3_RV4 (Pr0335)
            Region: lacI
            Sequence file: RDB13504_A7Ctf.seq check
            >13504_13504_A7Ct_3_pGL3_Rv4_D05_11_ABI24.ab1
                1 CTCAACGATG GGTTGATGCT CTCAGGGCAT CGGTCGAGAT CCCGGTGCCT AATGAGTGAG
               61 CTAACTTACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG
              121 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCCA
              181 GGGTGGTTTT TCTTTTCACC AGTGAGACGG GCAACAGCTG ATTGCCCTTC ACCGCCTGGC
              241 CCTGAGAGAG TTGCAGCAAG CGGTCCACGC TGGTTTGCCC CAGCAGGCGA AAATCCTGTT
              301 TGATGGTGGT TAACGGCGGG ATATAACATG AGCTGTCTTC GGTATCGTCG TATCCCACTA
              361 CCGAGATGTC CGCACCAACG CGCAGCCCGG ACTCGGTAAT GGCGCGCATT GCGCCCAGCG
              421 CCATCTGATC GTTGGCAACC AGCATCGCAG TGGGAACGAT GCCCTCATTC AGCATTTGCA
              481 TGGTTTGTTG AAAACCGGAC ATGGCACTCC AGTCGCCTTC CCGTTCCGCT ATCGGCTGAA
              541 TTTGATTGCG AGTGAGATAT TTATGCCAGC CAGCCAGACG CAGACGCGCC GAGACAGAAC
              601 TTAATGGGCC CGCTAACAGC GCGATTTGCT GGTGACCCAA TGCGACCAGA TGCTCCACGC
              661 CCAGTCGCGT ACCGTCTTCA TGGGAGAAAA TAATACTGTT GATGGGTGTC TGGTCAGAGA
              721 CATCAAGAAA TAACGCCGGA ACATTAGTGC AGGCAGCTTC CACAGCAATG GCATCCTGGT
              781 CATCCAGCGG ATAGTTAATG ATCAGCCCAC TGACGCGTTG CGCGAGAAGA TTGTGCACCG
              841 CCGCTTTACA GGCTTCGACG CCGCTTCGTT CTACCATCGA CACCACCACG CTGGCACCCA
              901 GTTGATCGGC GCGAGATTTA ATCGCCGCGA CAATTTGCGA CGCGCGTGCA GGGCCAGACT
              961 GGAGTGGCAC GCCCATCAGC CACCGACTGT TGCCGCCAGT GTGTGCCACG CCATGGGATG
             1021 TATTCAGCTT CGGCCATCGC GGCATCCACT TTTCTGCGAT TTCGCAGAAT CGTGGCTTGG
            1081 CCGTTTGGAT TCAC
            //
            Primer: lacIpro_F (Pr0409)
            Region: lacI
            Sequence file: RDB13504_A7Ctg.seq check
            >13504_13504_B0BaF_lacIpro_F_F02_17_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGNNNNNNN NNNNNNNGNN NNGNNNNNAA TGNNNNNACC
               61 AGTAACGTTA TACGATGNNN NNNNNAGTAT GCCGGTGTCT CTTATCAGAC CGTTTCCCGC
              121 GTGGTGAACC AGGCCAGCCA CGTTTCTGCG AAAACGCGGG AAAAAGTGGA AGCGGCGATG
              181 GCGGAGCTGA ATTACATTCC CAACCGCGTG GCACAACAAC TGGCGGGCAA ACAGTCGTTG
              241 CTGATTGGCG TTGCCACCTC CAGTCTGGCC CTGCACGCGC CGTCGCAAAT TGTCGCGGCG
              301 ATTAAATCTC GCGCCGATCA ACTGGGTGCC AGCGTGGTGG TGTCGATGGT AGAACGAAGC
              361 GGCGTCGAAG CCTGTAAAGC GGCGGTGCAC AATCTTCTCG CGCAACGCGT CAGTGGGCTG
              421 ATCATTAACT ATCCGCTGGA TGACCAGGAT GCCATTGCTG TGGAAGCTGC CTGCACTAAT
              481 GTTCCGGCGT TATTTCTTGA TGTCTCTGAC CAGACACCCA TCAACAGTAT TATTNNNNNN
              541 CATGAAGACG GTACGCGACT GGGCGTGGAG CATCTGGTCG CATTGGGTCA CCAGCAAATC
              601 GCGCTGTTAG CGGGCCCATT AAGTTCTGTC TCGGCGCGTC TGCGTCTGGC TGGCTGGCAT
              661 AAATATNNCN NNCGCAATCA AATTCAGCCG ATAGCGGAAC GGGAAGGCGA CTGGAGTGCC
              721 ATGTCCGGTT TTCAACAAAC CATGCAAATG CTGAATGAGG GCATCGTTCC CACTGCGATG
              781 CTGGTTGCCN NCGATCAGAT GGCGCTGGGC GCAATGCGCG CCATTACNNA GTCCNGNCTG
              841 CGCGTTGGTG NGGACNTCTC GNNNNNGGNA NNNNANNANA CCGAAANANN GCTCATGTTA
              901 TNTCCCGCCG TTANCCNCNA TCNAANNAGG ANTTTNNCCT GCTGGGGGCA ANNCNNNNNG
              961 NACCGNNTNG CTGCAACTCT CTCNNGNNCN NNNNNNGAAN GGNCNNTCAN CNGNNGNCCN
            1021 NNNTCANTGN NGAAAANNAA AAANCNNNNN NNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Takeda, T.A., Continuous de novo biosynthesis of haem and its rapid turnover to bilirubin are necessary for cytoprotection against cell damage. Sci. Rep. 5: 10488 (2015). PMID 25990790. [link to RRC of NBRP]
            reference Iwatani, S., Fluorescent protein-based detection of unconjugated bilirubin in newborn serum. Sci. Rep. 6: 28489 (2016). PMID 27324682.
            reference Hieu Liem, P., A simple and highly sensitive method of measuring heme oxygenase activity. Biol. Chem. 396 (11): 1265-1268 (2015). PMID 26040009. [link to RRC of NBRP]
            reference Kumagai, A., A bilirubin-inducible fluorescent protein from eel muscle. Cell 153 (7): 1602-1611(2013). PMID 23768684. [link to RRC of NBRP]
            review Miyawaki, A., Exploiting the cyanobacterial light-harvesting machinery for developing fluorescent probes. Nat. Methods 13 (9): 729-730 (2016). PMID 27575623.

            2023.08.18

            GNP_filter3_RDBDEP_html_230702.pl