Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET28/EqtII(8-69)-EGFP-His6

Marker of sphingomyelin in cell membrane. Bacterial expression plasmid of nontoxic Eqinatoxin II.

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Makino, A., FASEB J. 29 (2): 477-493, 2015)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Makino, A., FASEB J. 29 (2): 477-493, 2015)。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13497_B1L6p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET_lacIpro_R (Pr0838) Region: lacO,RBS,insert 5'(EqtII(8-69) 5') Sequence file: RDB13497_B1L6d.seq
>13497_13497_B2KfS_pET_lacIpro_R_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNTNNCNNT AGGGGNNNTG TGAGCGGANA ACAATTCCCC TCTAGAAATA    61 ATTTTGTTTA ACTTTAAGAA GGAGATATAC CGGTACCATG AGCGCGGATG TGGCTGGTGC   121 ATGCATAGAT GGCGCCTCTC TGAGTTTCGA CATCCTCAAG ACCGTGTTGG AAGCTCTGGG   181 GAATGTCAAA CGGAAGATTG CCGTAGGAGT GGACAACGAA AGCGGTAAGA CATGGACTGC   241 CCTGAATACG TACTTCAGGT CTGGAACCAG CGACATTGTT CTGCCCCACA AAGTCCCACA   301 TGGGTGTGCA CTCCTGTACA ATGGGCAGAA AGATCGAGGA CCTGTTGCGA CTGGTGCAGT   361 TGGGGTATTG GCCTATCTCA TGTCAGATGG CAACACCCTT GCTGTGCTGT TTTCCGTCCC   421 CTATGACTAC AACTGGTATT CCAACTGGTG GAATGTGCGC ATCTACAAGG GCAAACGTAG   481 AGCCGATCAA CGCATGTATG AGGAGCTTTA CTACAACCTG TCCCCGTTTA GGGGCGACAA   541 TGGATGGCAC ACACGGAATC TCGGGTATGG CCTGAAGAGC AGANGCTTCA TGAACAGCAG   601 TGGCCATGCC ATCCTGGAGA TTCNCGTGTC CNANGCTGGT GGANGGNCNN GCGGCGGNNC   661 NNNNNTCNNA NGANNANNNN NNNANNCNTN NNNAGNNNNN NANNNNCTGN NNNNNNGGGN 721 NNNNNCNNNN NNNNNN //
Primer: T7 terminator (Pr0155) Region: 6xHis,insert 3'(EGFP 3') Sequence file: RDB13497_B1L6e.seq
>13497_13497_B1L6_T7terminater_C02_08_ABI24.ab1     1 NNNNNNNNNT NNNNNCTTTG TNNGCNGCCG GATCTCAGTG GTGGTGGTGG TGGTGAAGCT    61 TCTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC TCCAGCAGGA   121 CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC GGACTGGGTG CTCAGGTAGT   181 GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG TAGTGGTCGG   241 CGAGCTGCAC GCTGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTCACC TTGATGCCGT   301 TCTTCTGCTT GTCGGCCATG ATATAGACGT TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT   361 GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG CGGTTCACCA   421 GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA   481 AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG TCGTGCTGCT   541 TCATGTGGTC GGGGTAGCGG CTGAAGCACT GCACGCCGTA NGTCAGGGTG GTCACGANGG   601 TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCNNGNTC ANCTTGNCNT   661 ANGTGGNATC GCCCTCGCCN TCGNNNNANN CGCTGAANTN GNGGNNNNTN ACNTCNNNNN 721 CNNNCTCNAN NNNGNNGGNN NNNNNCCNNN NNNNNNN //
Primer: EGFP-N_R2 (Pr0905) Region: insert mid(EGFP 5',EqtII(8-69) 3') Sequence file: RDB13497_B1L6f.seq
>13497_13497_B2KfS_EGFP-N_R2_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNCNNNNNN CTGCACGCCG TAGGTCAGGG TGGTCACGAG GGTGGGCCAG    61 GGCACGGGCA GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCAGCTTGCC ATAGGTGGCA   121 TCGCCCTCGC CCTCGCCGGA CACGCTGAAC TTGTGGCCGT TTACGTCGCC GTCCAGCTCG   181 ACCAGGATGG GCACCACCCC GGTGAACAGC TCCTCGCCCT TGCTCACCAT GGATCCTCCT   241 CCTCCTCCTC CGAATTCAGA TCCGCCGCCT GACCCTCCAC CAGCCTTGGA CACGTGAATC   301 TCCAGGATGG CATGGCCACT GCTGTTCATG AAGCCTCTGC TCTTCAGGCC ATACCCGAGA   361 TTCCGTGTGT GCCATCCATT GTCGCCCCTA AACGGGGACA GGTTGTAGTA AAGCTCCTCA   421 TACATGCGTT GATCGGCTCT ACGTTTGCCC TTGTAGATGC GCACATTCCA CCAGTTGGAA   481 TACCAGTTGT AGTCATAGGG GACGGAAAAC AGCACAGCAA GGGTGTTGCC ATCTGACATG   541 AGATAGGCCA ATACCCCAAC TGCACCAGTC GCAACAGGTC CTCGATCTTT CTGCCCATTG   601 TACAGGANTG CACACCCATG TGGGANTTTG TGGGGCANAA NAATGTCGCT GGNTCCNGAN   661 CTGAANTACG TATTCNNGGC ANTCNNTGNC TTANCGCTTT CGTNGNCNNN NNCTANNGCA 721 ANCNNNNNNT NNN //
Primer: pGL3_RV4 (Pr0335) Region: lacI Sequence file: RDB13497_B1L6g.seq
>13497_13497_B2KfS_pGL3_RV4_F01_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTCTCN NGGGCATCGG TCGANGATCC CGGTGCCTAA    61 TGAGTGANGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA   121 CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT   181 TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA   241 CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA   301 AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT   361 ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG   421 CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA   481 GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA   541 TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC NGCCNGACGC ANACGCGCCG   601 ANACAGAACT TAATGNGCCC GCTAACAGCG CGANTTGCTG GTGANCCAAT GCGACCAGAT   661 GCTCCNCGCC CNGTCNCGNA NCGTCNNCNT GGGNNAAAAN NNTANTGNTG ANGGGTGNCN 721 GNNCNNNNNN NTNNANAANN NNNNNCNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

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