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pLGFPm

Bacterial expression plasmid of lgfp, IPTG-inducible. p15A origin and ampicillin resistance.

Catalog number RDB13449
Resource name pLGFPm
Clone info. Bacterial expression plasmid of lgfp, IPTG-inducible. p15A origin and ampicillin resistance.
Comment lgfp: The gfpmut3 gene, changed its all codons to the lowest usage in E. coli (accession number, LC018331).
Vector backbone pTAK132 (plasmid)
Size of vector backbone 5.0 kb
Selectable markers ampicillin (E. coli)
Gene/insert name Aequorea victoria green fluorescent protein (GFP) cDNA
Reference of insert sequence LC018342 (DDBJ accession number)
Depositor|Developer Kobayashi, Hideki |
Other clones in our bank
External Database
Aequorea victoria green fluorescent protein (GFP)
            Reference sequence
              LC018331
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Kobayashi, H., Appl Environ Microbiol. 81 (7): 2544-53, 2015)

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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kobayashi, H., Appl Environ Microbiol. 81 (7): 2544-53, 2015)。
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            RDB13449 pLGFPm Under QC test. Please contact us. DNA solution

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References and tips

            Original reference

            original Kobayashi, H., Inducible suppression of global translation by overuse of rare codons. Appl. Environ. Microbiol. 81 (7): 2544-2553 (2015). PMID 25636849.

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

            References

            Original, user report and related articles

            original Kobayashi, H., Inducible suppression of global translation by overuse of rare codons. Appl. Environ. Microbiol. 81 (7): 2544-2553 (2015). PMID 25636849.

            2023.04.10

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