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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1/ENGase/FLAG-His

Expression clone of mouse ENGase.

Catalog number RDB13417
Resource name pcDNA3.1/ENGase/FLAG-His
Clone info. Expression clone of mouse ENGase.
Comment V5 tag in the original vector was replaced by a FLAG tag.
Vector backbone pcDNA3.1/V5-HIS (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells)
Gene/insert name Mouse ENGase
Depositor|Developer Suzuki, Tadashi |
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External Database
Mouse ENGase

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Proc. Natl. Acad. Sci. U.S.A., 112 (5): 1398-1403 (2015))
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          提供条件 利用者は提供承諾書を用いて、事前に寄託者の承認を得る。 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Proc. Natl. Acad. Sci. U.S.A., 112 (5): 1398-1403 (2015))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          備考 【追加提供依頼書】承諾書(FormD)
          寄託者の書面による提供承諾が必要です。

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          RDB13417 pcDNA3.1/ENGase/FLAG-His DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Huang, C., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc. Natl. Acad. Sci. U.S.A. 112 (5): 1398-1403 (2015). PMID 25605922.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13417_A5E1p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13417_A5E1a.seq check
          >13417_13417_2_CMV_Forward_A08_02.ab1
              1 CGTTTCCCTC CGCCCATTGA CGCAATGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
             61 AGCAGAGCTC TCTGGCTAAC TAGAGAACCC ACTGCTTACT GGCTTATCGA AATTAATACG
            121 ACTCACTATA GGGAGACCCA AGCTGGCTAG TTAAGCTTGG TACCGAGCTC GGATCCACTA
            181 GTCCAGTGTG GTGGAATTCT GCAGATATCC AGCACAGTGG CAGTCATGGA GACCTCGTCG
            241 GTGCTGACCC GGGGAGCTGC AAGGCAGCGG AGCCCCGCGG CCCCGGAGAA GCAGGCGCGG
            301 GACCAGACGG AGCGGAGGCC GGGGCGGCGG AGGCAGGGGC GGAGGATCAA CGAGGATCAA
            361 GAGGAGGAAG CAGTCTTTAG AGAGGTGGTC AGTTTCACCC CGGATCCTTT ACCAGCTAGA
            421 TATTATGACA AGGATACTAC CAGACCTATC AGCTTTTACC TGTCGACGCT GGAGGAGCTC
            481 CTGGCCTGGA CCCCGCTCAT GGAGGATGGC TTCAATGTAG CCCTGGAACC CCTGGTGTGT
            541 CGCCGGCCTC CACTCAGCAG CCCCAGGCCC CGGACCCTGC TGTGCCACGA CATGATGGGT
            601 GGTTACCTGG AGGACAGGTT CATTCAGGGC TCAGAGGTGC AGAACCCCTA TTCCTTCTAC
            661 CACTGGCAGT ATATCGACAT CTTTGTGTAC TTCAGCCACC ACACGGTCAC CATCCCCCCA
            721 GTGTGCTGGA CCAATGCCGC ACACAGGCAC GGGGTCTGCG TGCTGGGGAC CTTCATCACA
            781 GAGTGGCAAG AGGGAGGCAG GCTCTGCGAA GCCTTCCTGG CCGGGGATGA GCCCTCCTTC
            841 CAGGCCGTGG CCGATCGGCT GGTCCAGATT GCTCAGTTCT TTCGTTTTGA CGGCTGGCTC
            901 ATCAACATCG AGAACTCCCT GACTCCAGCT GCCGTGAGGA ACACACCTTT TATTTCTCCA
            961 GTACCTTACC GCACAGCTCC ATCAGCAGGT CCCCCGGAGG TCTGGTGCTG TGGTACGACA
           1021 GTGTGGTACA GAGCGGGGCA GCTCAGTGGC AGGATGAACT TGCATGATCA GGAACCCGGG
          1081 TCCTTCTTTG ACTCCCTGCC GGAAATGGCC CATC
          //
          Primer: BGH_rev
          Sequence file: RDB13417_A5E1b.seq check
          >13417-13417-_2_BGH-rev_D03_12.ab1
              1 GGCTTTCTTA ATGGTGATGG TGATGATGAC CGGTACGCTT ATCGTCGTCA TCCTTGTAAT
             61 CCTGAGGGAC CGAGAAGAGC AGGACCGCCT TTCCCCACTC AGCCTGGGGC ACCAGGAAAC
            121 CCTCCCTGGG GACAGGCTCC ACCAGGAACT CCACACGACC ATCCTGCCCA AATCCAGCAG
            181 CCTCCACAGC CAAGTCCACC ACACGGTACT GGTTGGCGAA AGCCAGGCCC AGGAACGTGG
            241 GCTTCTCTGT CTCTGGGGAC TCTGCCACAG AGCTGCTGCT TCCTGTCCGT TTCCAGCAGT
            301 GAATGCGGAA GCAGCGGGCT CGGAGCAGCA GGTAGGACCA GTGCAGGGTG CAGCTGAGGA
            361 GGAGCCGGGT AGGGAGGCCC TCAGACCCGG TGATCAGAGG AAGCCATCGT ATCTGAGAAA
            421 TGGTGACATT CTGCACCCGG GGCAGAGGGG CCAGAAGGCT GCTGGCATCA ACCACCTGGA
            481 TCTCTCCAAG GCGACAAATG AAGCTCTCCT CCTCCCGGCT GCCTGGCGGC CGTGAGAAGC
            541 TCACCAAGAG GTCCTGGAGC AGGCACCCAC GAAGGTTTAC CTCATAGCAG CGTTGAATCC
            601 AGCCCCCACT GAGCTGCTGG CCACAACTGC TGGCCCATCT GGCCAGCCTG GTGGGGGGTA
            661 CCCGGAGGGG TCTGGGGCTG TGCCGTGAGC CAGTTTCATT TAACACCAAC ATGCCACCCA
            721 CATGACAGCT GCTGGCATCC CCTGTTGTGA GCTCCAAAGC CACCTGGACA TCTGTTGAGC
            781 CTTCAAACTT GTACACCATG GACAGGAAAA CCTTGGGTGG CACTGGGATG TGCAGAGAGA
            841 ACAGCCTCAC AGCTACACTG TCAACCTCAG GCGGGATCAG GCCCCGGAGC AGCAGAGAGC
            901 TGCCCCCGTG CCAGGCATCA GTCAGGCAGC AGTGCGTCTT CACCCAGCCC CGACTGTCAC
            961 CAGCCAGCTT ATGTTCACCA AAGACGGGCT GTGTCTCCCT GGGCGCTAGG GTGGTACCAG
           1021 GGCCCCCACT GCTTTGCTCT TTGCCGTAGC AGACTCTTTC GAGTACCCAG GGCCCAGCAG
          1081 AAAGGAGTTG ACGATGCCAG AAGCAGAATA CTGTGTGTGG GAAGGAGGCC GGCT
          //
          Primer: SV40pro_F
          Sequence file: RDB13417_A5E1c.seq check
          >13417-13417_2_SV40pro_F_E03_15.ab1
              1 CCTGTCTGGG CATTCAGAGT AGTGAGGAGG CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA
             61 GCTCCCGGGA GCTTGTATAT CCATTTTCGG ATCTGATCAA GAGACAGGAT GAGGATCGTT
            121 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT
            181 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT
            241 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA
            301 ACTGCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC
            361 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG
            421 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC
            481 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA
            541 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA
            601 CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGCGCATGCC
            661 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA
            721 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA
            781 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT GGCGGCGAAT GGGCTGACCG
            841 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT
            901 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGTTTCGCG AATGACCGAC CCAAGCGACG
            961 CCCAACCTGC CATCACGAGA TTTCGATTTC CACCGCCGCT TCTATGGAAC GTGGGCTTCG
           1021 ATCGTTTTCA GGGAACGCGC TGAATGATTC TCCAGCGCGG GATCTCATGC CTTGAGTCAT
          1081 CGCCACTCAC CTGTATGCAG CTATATGTTA CAA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Huang, C., Endo-beta-N-acetylglucosaminidase forms N-GlcNAc protein aggregates during ER-associated degradation in Ngly1-defective cells. Proc. Natl. Acad. Sci. U.S.A. 112 (5): 1398-1403 (2015). PMID 25605922.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl