Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEF/RTAdeltaN236Q/V5-myc/ER

Expression clone of Ricin A chain nontoxic mutant (RTAdelta). N236Q substitution.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Proc. Natl. Acad. Sci. U.S.A., 112 (5): 1398-1403 (2015))
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form by depositor [Word]
Remarks	((Additional form)) Approval Form(FormD) Please obtain written approval of the depositor.

提供案内 (日本国内) [open/close]

提供条件	利用者は提供承諾書を用いて、事前に寄託者の承認を得る。 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Proc. Natl. Acad. Sci. U.S.A., 112 (5): 1398-1403 (2015))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 寄託者による提供承諾書 [Word]
備考	【追加提供依頼書】承諾書(FormD) 寄託者の書面による提供承諾が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13414_A5E1p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F Sequence file: RDB13414_A5E1a.seq
>13414-13414_2_EF1a-C_F_B02_05.ab1     1 TCAGGGGATT GCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT    61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAACACGTG GCCACCATGG GATGGAGCTG   121 TATCATCCTC TTCTTGGTAG CAACAGCTAC AGGTAAGGGG TTAACAGTAG CAGGCTTGAG   181 GTCTGGACAT ATATATGGGT GACAATGACA TCCACTTTGC CTTTCTCTCC ACAGGCGCGC   241 ACTCCCAGGT CCAACTGCAG ATATTCCCCA AACAATACCC AATTATAAAC TTTACCACAG   301 CGGGTGCCAC TGTGCAAAGC TACACAAACT TTATCAGAGC TGTTCGCGGT CGTTTAACAA   361 CTGGAGCTGA TGTGAGACAT GAAATACCAG TGTTGCCAAA CAGAGTTGGT TTGCCTATAA   421 ACCAACGGTT TATTTTAGTT GAACTCTCAA ATCATGCAGA GCTTTCTGTT ACATTAGCCC   481 TGGATGTCAC CAATGCATAT GTGGTCGGCT ACCGTGCTGG AAATAGCGCA TATTTCTTTC   541 ATCCTGACAA TCAGGAAGAT GCAGAAGCAA TCACTCATCT TTTCACTGAT GTTCAAAATC   601 GATATACATT CGCCTTTGGT GGTAATTATG ATAGACTTGA ACAACTTGCT GGTAATCTGA   661 GAGAAAATAT CGAGTTGGGA AATGGTCCAC TAGAGGAGGC TATCTCAGCG CTTTATTATT   721 ACAGTACTGG TGGCACTCAG CTTCCAACTC TGGCTCGTTC CTTTATAATT TGCATCCAAA   781 TGATTTTCCA ATATATTGAG GGAGAAATGC GCACGAGAAT TAGGTACAAC CGGAGATCTG   841 CACCAGATCC TAGCGTAATT ACACTTGAGA ATAGTTGGGG GAGACTTTCC ACTGCAATTC   901 AAGAGTCTAA CCAAGGAGCC TTTGCTAGTC CAATTCAACT GCAAAGACGT CAAGGTTCCA   961 AATTCAGTGT GTACGATGTG AGTATATTAA TCCCTATCAT AGCTCTCATG GGTGTATAGA  1021 TGCGCACCTC CACCCATCGT CACAGTTTTC TCGAGATCAA ACGGGGGTAA GCCTATCCCT 1081 ATCTCTCATC CGATCTCGAT TCTACCGGAC CGCAGAAAGC AAAAAAAACG TA //
Primer: BGH_rev Sequence file: RDB13414_A5E1b.seq
>13414-13414_2_BGH-rev_C02_08.ab1     1 GTTACAGAAT CAGCTCGTGC TTCTCGCTTG CGGCCCCATT CAGATCCTCT TCTGAGATGA    61 GTTTTTGTTC TGCGGCCGTA GAATCGAGAC CGAGGAGAGG GTTAGGGATA GGCTTACCCC   121 GTTTGATCTC GAGAAACTGT GACGATGGTG GAGGTGCGCA TCTATACACC ATGAGAGCTA   181 TGATAGGGAT TAATATACTC ACATCGTACA CACTGAATTT GGAACCTTGA CGTCTTTGCA   241 GTTGAATTGG ACTAGCAAAG GCTCCTTGGT TAGACTCTTG AATTGCAGTG GAAAGTCTCC   301 CCCAACTATT CTCAAGTGTA ATTACGCTAG GATCTGGTGC AGATCTCCGG TTGTACCTAA   361 TTCTCGTGCG CATTTCTCCC TCAATATATT GGAAAATCAT TTGGATGCAA ATTATAAAGG   421 AACGAGCCAG AGTTGGAAGC TGAGTGCCAC CAGTACTGTA ATAATAAAGC GCTGAGATAG   481 CCTCCTCTAG TGGACCATTT CCCAACTCGA TATTTTCTCT CAGATTACCA GCAAGTTGTT   541 CAAGTCTATC ATAATTACCA CCAAAGGCGA ATGTATATCG ATTTTGAACA TCAGTGAAAA   601 GATGAGTGAT TGCTTCTGCA TCTTCCTGAT TGTCAGGATG AAAGAAATAT GCGCTATTTC   661 CAGCACGGTA GCCGACCACA TATGCATTGG TGACATCCAG GGCTAATGTA ACAGAAAGCT   721 CTGCATGATT TGAGAGTTCA ACTAAAATAA ACCGTTGGTT TATAGGCAAA CCAACTCTGT   781 TTGGCAACAC TGGTATTTCA TGTCTCACAT CAGCTCCAGT TGTTAAACGA CCGCGAACAG   841 CTCTGATAAA GTTTGTGTAG CTTTGCACAG TGGCACCCGC TGTGGTAAAG TTTATAATTG   901 GGTATTGTTT GGGGGAATAT CTGCAGTTGG ACCTGGGAGT GCGCGCCTGT GGAGAGAAAG   961 GCAAAGTGGA TGTCATTGTC ACCCATATAT ATGTCCAGAC CCTCAAGCCC TGCTACTGTT  1021 TAACCCCCTT ACCCTGTAGC TGTTTGCTAC CCAAAGAAAG AGGATGGATA CAGGCTCCAA  1081 TCCCCATGGA TGCAACGTGT CAACGACACC TGAAAATGGT AGGAAAAAAA AACCTTTTGG 1141 CTAC //
Primer: SV40pro_F Sequence file: RDB13414_A5E1c.seq
>13414-13414_2_SV40pro_F_D02_11.ab1     1 ACTGTCGGTG GCATTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA    61 AGCTCCCCCC CCGGGAGGTC CACAATGGTT GAACAAGATG GATTGCACGC AGGTTCTCCG   121 GCCGCTTGGG TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT   181 GATGCCGCCG TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC   241 CTGTCCGGTG CCCTGAATGA ACTCCAGGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG   301 ACGGGCGTTC CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG   361 CTATTGGGCG AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA   421 GTATCCATCA TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA   481 TTCGACCACC AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT   541 GTCGATCAGG ATGATCTGGA CGAAGAGCAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC   601 AGGCTCAAGG CGCGTATGCC CGACGGCGAG GATCTCGTCG TGACTCATGG CGATGCCTGC   661 TTGCCGAATA TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG   721 GGTGTGGCGG ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAGCTT   781 GGCGGCGAAT GGGCTGACCG CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG   841 CGCATCGCCT TCTATCGCCT TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGGTTCGAA   901 ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCCTT   961 CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC GGGCTGGGAT GATCCTCCAG  1021 CGCGGGGATC TCATGCTTGG AGTCCTCCGC CACCCCAACT GGTTATGGCA GCCTTATAAT  1081 TGATACGATA AAGCCAATAG CCATTCACGA AGTTCACTAA TAAAGGCATT CACGTGCAAT 1141 TCCGTTAGCG ATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl