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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-SOD1_G93A

Mammalian expression vector for human G93A SOD1 with EGFP tag at C-terminus.

Catalog number RDB13392
Resource name pEGFP-SOD1_G93A
Clone info. Mammalian expression vector for human SOD1 with EGFP tag at C-terminus. G93A mutation correlating familial ALS.
Comment Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-GFP antibodies, and observation by fluorescent microscope. Known differences (at least): C to T substitution at 187nt (Gly, synonym) of NM_000454.4.
Vector backbone pEGFP-N3 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |
Other clones in our bank
External Database
human Superoxide dismutase 1
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FASEB J., 22(7), 2476-2487 (2008))
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FASEB J., 22(7), 2476-2487 (2008))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB13392 pEGFP-SOD1_G93A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Urushitani, M., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13392_A5Edp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Sequence file: RDB13392_A5Eda.seq check
          >13392_13392_A9G8F_2_CMV-Forward_E03_15_ABI24.ab1
              1 TCCGCCCCCA TTTGACGCAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT ATATAAGCAG
             61 AGCTGGTTTA GTGAACCGTC AGATCCGCTA GCGCTACCGG ACTCAGATCT CGAGCTCAAG
            121 CTTCGAATTC GCCATGGCGA CGAAGGCCGT GTGCGTGCTG AAGGGCGACG GTCCAGTGCA
            181 GGGCATCATC AATTTCGAGC AGAAGGAAAG TAATGGACCA GTGAAGGTGT GGGGAAGCAT
            241 TAAAGGACTG ACTGAAGGCC TGCATGGATT CCATGTTCAT GAGTTTGGAG ATAATACAGC
            301 AGGCTGTACC AGTGCAGGTC CTCACTTTAA TCCTCTATCC AGAAAACACG GTGGGCCAAA
            361 GGATGAAGAG AGGCATGTTG GAGACTTGGG CAATGTGACT GCTGACAAAG ATGCTGTGGC
            421 CGATGTGTCT ATTGAAGATT CTGTGATCTC ACTCTCAGGA GACCATTGCA TCATTGGCCG
            481 CACACTGGTG GTCCATGAAA AAGCAGATGA CTTGGGCAAA GGTGGAAATG AAGAAAGTAC
            541 AAAGACAGGA AACGCTGGAA GTCGTTTGGC TTGTGGTGTA ATTGGGATCG CCCAAGGATC
            601 CATCGCCACC ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT
            661 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA
            721 TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC
            781 CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA
            841 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG
            901 CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG
            961 CGACACCCTG GTGAACCGCA TCGAGCTGAA AGGCATCGAC TTCAAGAGGG ACGGCAACAT
           1021 CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCAACAA CGGTCTATTA TCATTGGCCG
           1081 ACAAGCAGAA GAAACCGCAT CCAAGTGACC TTCAAGGATC GGCACAACAT CGAAGGGAAC
          1141 GGGCCAGACG CGGGG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB13392_A5Edb.seq check
          >13392_13392_A9G8F_2_SV40pro_F_V2_G08_07_ABI08.ab1
              1 TAGGAGCTTT TTTTGGGAAG GGCTTAGGGC TTTTGCAAAG ATCGATCAAG AGACAGGATG
             61 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT
            121 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT
            181 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC
            241 CCTGAATGAA CTGCAAGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC
            301 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
            361 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT
            421 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA
            481 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA
            541 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC
            601 GAGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT
            661 CATGGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGGCG
            721 GGACCGCTAT CAGGACATAG CGTTGGCTAC CCGTGATATT TGCCTGAAAG AGCTTGGCGG
            781 CGAATGGGGC TGGACCCGCT TCCCTCGTGC TTTTACCGTT ATCGCCGCTC CCCGATTTCC
            841 GCAACGCCAT CGCCCTTCTA TCCGCCTTCT TGACAAAGTT TCTTCTGAAG CCGGGGACTT
          901 TGGGGGGTTT CGAAAATGAA CCGAACCAAA C
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Urushitani, M., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl