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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-SOD1_G85R

Mammalian expression vector for human G85R SOD1 with EGFP tag at C-terminus.

Catalog number RDB13391
Resource name pEGFP-SOD1_G85R
Clone info. Mammalian expression vector for human SOD1 with EGFP tag at C-terminus. G85R mutation correlating familial ALS.
Comment Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-GFP antibodies, and observation by fluorescent microscope.
Vector backbone pEGFP-N3 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |
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External Database
human Superoxide dismutase 1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FASEB J., 22(7), 2476-2487 (2008))
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FASEB J., 22(7), 2476-2487 (2008))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB13391 pEGFP-SOD1_G85R DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Urushitani, M., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13391_A5Es.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13391_A5Esa.seq check
          1
          Primer: EGFP_N_rev
          Sequence file: RDB13391_A5Esb.seq check
          1
          Primer: SV40pro_F_V2
          Sequence file: RDB13391_A5Esc.seq check
          1

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Urushitani, M., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461.

          2023.04.10

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