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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEGFP-SOD1_WT

Mammalian expression vector for human SOD1 with EGFP tag at C-terminus.

Catalog number RDB13390
Resource name pEGFP-SOD1_WT
Clone info. Mammalian expression vector for human WT OD1 with EGFP tag at C-terminus.
Comment Expression was confirmed by the depositor with Westernblotting of transfected mammalian cells using commarcialy available anti-SOD1 and anti-GFP antibodies, and observation by fluorescent microscope. Known differences (at least): C to T substitution at 187nt (Gly, synonym) of NM_000454.4.
Vector backbone pEGFP-N3 (Plasmid)
Size of vector backbone 4.7 kb
Selectable markers Kan^r (E. coli), Neo^r (mammalian cells)
Gene/insert name human Superoxide dismutase 1 cDNA
Depositor|Developer Urushitani, Makoto |
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External Database
human Superoxide dismutase 1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (FASEB J., 22(7), 2476-2487 (2008))
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (FASEB J., 22(7), 2476-2487 (2008))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB13390 pEGFP-SOD1_WT DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Urushitani, M., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461.

          Further references such as user reports and related articles (go to bottom)

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          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13390_A6Arp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Sequence file: RDB13390_A6Ara.seq check
          >13390_13390_A9G8F_2_CMV-Forward_B03_06_ABI24.ab1
              1 TCCGTCCCAT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT ATAAGCAGAG
             61 CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTACCGGAC TCAGATCTCG AGCTCAAGCT
            121 TCGAATTCGC CATGGCGACG AAGGCCGTGT GCGTGCTGAA GGGCGACGGT CCAGTGCAGG
            181 GCATCATCAA TTTCGAGCAG AAGGAAAGTA ATGGACCAGT GAAGGTGTGG GGAAGCATTA
            241 AAGGACTGAC TGAAGGCCTG CATGGATTCC ATGTTCATGA GTTTGGAGAT AATACAGCAG
            301 GCTGTACCAG TGCAGGTCCT CACTTTAATC CTCTATCCAG AAAACACGGT GGGCCAAAGG
            361 ATGAAGAGAG GCATGTTGGA GACTTGGGCA ATGTGACTGC TGACAAAGAT GGTGTGGCCG
            421 ATGTGTCTAT TGAAGATTCT GTGATCTCAC TCTCAGGAGA CCATTGCATC ATTGGCCGCA
            481 CACTGGTGGT CCATGAAAAA GCAGATGACT TGGGCAAAGG TGGAAATGAA GAAAGTACAA
            541 AGACAGGAAA CGCTGGAAGT CGTTTGGCTT GTGGTGTAAT TGGGATCGCC CAAGGATCCA
            601 TCGCCACCAT GGTGAGCAAG GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG
            661 AGCTGGACGG CGACGTAAAC GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG
            721 CCACCTACGG CAAGCTGACC CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT
            781 GGCCCACCCT CGTGACCACC CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC
            841 ACATGAAAGC AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGG TCCAGGAGCG
            901 CACCATCTTC TTCAAGGGAC GACGGGCAAC TACAAGACCC GGCGCCCGAG GTGAAGTTCG
            961 AGGGCGACAC CCTGGGTGAA CCGGCATCGG AGCTGAAAGG GCATCGGACT TTCAAGGGAG
           1021 GACGGGCAAC ATCCTGGGGG CACAAGGCTG GGAAGTTACA CTACACAGGC CCACAAACGT
           1081 CTATATCATG GGCCGGACAG GCAGGGAGGG AACGGGCCAT TCCAGGTTGA ACCTTCAAGG
          1141 ATTCCGGCCA CAAACCAATT CGGAAGGGAA CGGAGCGAGG GCGGAGTGTG CCATG
          //
          Primer: SV40pro_F (Pr0110)
          Region: SV40 pro_ori,NeoR_KanR
          Sequence file: RDB13390_A6Arb.seq check
          >13390_13390_A6Ar_2_SV40pro_F_A06_03_ABI24.ab1
              1 CTTAACCATT CCAGAGTAGT GAGGAGGCTT TTTTGGAGGC CTAGGCTTTT GCAAAGATCG
             61 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT
            121 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT
            181 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
            241 CCGACCTGTC CGGTGCCCTG AATGAACTGC AAGACGAGGC AGCGCGGCTA TCGTGGCTGG
            301 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT
            361 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG
            421 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT
            481 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
            541 GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT
            601 TCGCCAGGCT CAAGGCGAGC ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG
            661 CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC
            721 GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG
            781 AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT
            841 CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT
            901 TCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG
            961 CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCCGCCTG GGATGATCCT
           1021 CCAGCGCGGG GAATCTCATG CCTGGGAGTT CTTCGCCCCA CCCCTAAGGG GCAGCTAAAC
           1081 TTGAACAACG GAAAGAGAAA CAATACAGAA ATGAACCTCG CGGCCTAAGT GAACGTCAAT
          1141 TAAGATAAGA ACCAGAAAGT ATCA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Urushitani, M., The endoplasmic reticulum-Golgi pathway is a target for translocation and aggregation of mutant superoxide dismutase linked to ALS. FASEB J. 22 (7): 2476-2487 (2008). PMID 18337461.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl