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pUC-T7-HCV-PABP-HA

HCV IRES-dependent expression vector of human PABP

Catalog number RDB13281
Resource name pUC-T7-HCV-PABP-HA
Clone info. HCV IRES-dependent expression vector of human PABP.
Comment Expression was confirmed by depositor with Western blotting.
Vector backbone pUC-T7-HCV (Plasmid)
Size of vector backbone 3.4 kb
Selectable markers Amp^r
Gene/insert name Human PABP cDNA
Depositor|Developer Imataka, Hiroaki |
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External Database
Human PABP

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (J Biol Chem. 289(46): 31960-71, 2014)
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J Biol Chem. 289(46): 31960-71, 2014)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB13281 pUC-T7-HCV-PABP-HA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13281_A7Drp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Reverse2
          Sequence file: RDB13281_A7Dra.seq check
          >13281_13281_A7Dr_Reverse2_A12_03_ABI24.ab1
              1 CATGCATGCA TTACGCCAGC TTTATACGAC TCACTATAGG GAGACCGGCA GATCTGCCAG
             61 CCCCCGATTG GGGGCGACAC TCCACCATAG ATCACTCCCC TGTGAGGAAC TACTGTCTTC
            121 ACGCAGAAAG CGTCTAGCCA TGGCGTTAGT ATGAGTGTCG TGCAGCCTCC AGGACCCCCC
            181 CTCCCGGGAG AGCCATAGTG GTCTGCGGAA CCGGTGAGTA CACCGGAATT GCCAGGACGA
            241 CCGGGTCCTT TCTTGGATCA ACCCGCTCAA TGCCTGGAGA TTTGGGCGTG CCCCCGCGAG
            301 ACTGCTAGCC GAGTAGTGTT GGGTCGCGAA AGGCCTTGTG GTACTGCCTG ATAGGGTGCT
            361 TGCGAGTGCC CCGGGAGGTC TCGTAGACCG TGCACCATGA GCACGAATCC TAAACCTCAA
            421 AGAAAAACCG GATCCGAATT CCATATGAGA TCTGCTAGCG TCGACGATAT CCTCGAGTCT
            481 AGCATGAACC CCAGTGCCCC CAGCTACCCC ATGGCCTCGC TCTACGTGGG GGACCTCCAC
            541 CCCGACGTGA CCGAGGCGAT GCTCTACGAG AAGTTCAGCC CGGCCGGGCC CATCCTCTCC
            601 ATCCGGGTCT GCAGGGACAT GATCACCCGC CGCTCCTTGG GCTACGCGTA TGTGAACTTC
            661 CAGCAGCCGG CGGACGCGGA GCGTGCTTTG GACACCATGA ATTTTGATGT TATAAAGGGC
            721 AAGCCAGTAC GCATCATGTG GTCTCAGCGT GATCCATCAC TTCGCAAAAG TGGAGTAGGC
            781 AACATATTCA TTAAAAATCT GGACAAATCC ATTGATAATA AAGCACTGTA TGATACATTT
            841 TCTGCTTTTG GTAACATCCT TTCATGTAAA GGTGGTTTGT GATGAAAATG GTTCCAAAGG
            901 GCTACGGATT TGTACACTTT GAGACGCAGA AAGCAGCTGA AAGAGCTATT GAAAAAAATG
            961 AATGGAAATG CTCCTAAATG ATCGCAAAGT ATTTGTTGGG ACGATTTAAA GTCTCGTAAG
           1021 AACGAGAAGC TGAACTGCAG CTAGGCAAAA AGAATTCACC AATGGTTACA TCCAGCATTT
           1081 TGCAGAAGAC TGGATGAATG AAGCGCGTTA ACGATCTTCT TTGGGGCCTT GCCGTTAAAG
          1141 ACG
          //
          Primer: M13_-40
          Sequence file: RDB13281_A7Drb.seq check
          >13281_13281_A7Dr_M13(-40)_B12_06_ABI24.ab1
              1 CCGGTCAGGA TAGCGGCCGC GGATGATCCA AAAAACCCCT CAAGACCCGT TTAGAGGCCC
             61 CAAGGGGTTA TGCTAGTCTA GATGCATGCT CGAGCGGCCG CCAGTGTGAT GGATATCTGC
            121 AGAATTCCAG CACACTGGCG GCCGTTACTA GTGGATCCTT AGCCCGCATA GTCAGGAACA
            181 TCGTATGGGT AAACAGTTGG AACACCGGTG GCACTGTTAA CTGCTTTCTG GGCAGCCTCT
            241 TTAGCTTGGT GGGCTTGTAG TACAGCTACA GCTTCATCAA CCTTAGAACG GAGTGACTCT
            301 GGAGACTCGA GCATATGAAG AAGTTCTGAA TTATCAATCT CCAACAACAT GCCAGTGATT
            361 TTACCAGCAA GAGTAGGGTG CATGGCTTGA ATAAGAGGAA ACAGCCGTTC ACCCAACATT
            421 TGCTTTTGCT CTTGAGGAGG GGCAGATGCC AACATGGAAG CAGTCAAAGG TTCCTGACCT
            481 TGTACATGAA CAGCAGGCTG TTGCATTGTA ACTTGTGGCT GTGCATTAAG ATGTTGCTGA
            541 GGATTGCGAA CTCCTGCAGC ATATTTATAC TGTGGAACGG TGCGGACAGC AGGAGTAGCT
            601 GCAGCGGCTG CAGCTGCAGG ACGTGGACCC ATTGTCTGTG TTGATGTGTT AGCAACACGC
            661 TGTGTTGACA TGACTCGTGG AACCTGTGAA GAAGCTGGTC TCATAGTACT AAATGGTGGT
            721 CTAGGAGCAG CTGGGCGGAT AGCACCGGGC ATATTTTGGA ATGGATGAGG TCTGGCACCC
            781 TGAGCAGTCC AGCGAGGACT TGGTCTTAGT TGAGCAACTT GGCTAGGAGG ATAGTATGCA
            841 GCACGGTTCT GAGTCTGTGG GATAGCTGCC ATGAAGTAAC CTGAAGGAGG TGCTGGCTGG
            901 TAGGGGTTGA TTACAGGGTT GGGAACAGCT CGTACACTTG CCATTCTCTG CATATACTGG
            961 TTTAGTGAGG TGAGCCTGGC GCTCTTCTTT GCGCTGAGCT AAAGCTACAT ACAATGGGCT
           1021 TTGTGGCCAC AATTCTACCG ATCATTTCTG TAACTGCTTT AGTGGCTCCT CTGGGGAGAG
           1081 AAAACATACA ATCGAAATCG TTGGCCTTGC GACCACCCTC CAATTCAATA ACCGTTTTGC
          1141 ACT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Machida, K., A translation system reconstituted with human factors proves that processing of encephalomyocarditis virus proteins 2A and 2B occurs in the elongation phase of translation without eukaryotic release factors. J. Biol. Chem. 289 (46), 31960-31971 (2014) PMID 25258322.
          reference Imataka, H., Advantages of human cell-derived cell-free protein synthesis systems (in Japanese text). SEIKAGAKU 81 (4): 303-307 (2009). PMID 19462838.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl