Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCAP-Lamellipodin-His

Expression clone of mouse Lamellipodin cDNA

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Hayashi, S. et al., Developmental Cell 30 (6): 673-687, 2014). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	GFP is not included in the vector

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。(Hayashi, S. et al., Developmental Cell 30 (6): 673-687, 2014) 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学 宮崎純一博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	ベクターにGFPは含まない。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13050_A5A5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pCAG-F (Pr0328) Region: Chimeric intron,insert 5'(mLpd 5') Sequence file: RDB13050_A5A5a.seq
>13050_13050_B3C1F_3_pCAG-F_C02_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN GNGGGNNNGG GGNNNNGCGG GGTTCGGCTT    61 CTGGCGTGTG ACCGGCGGCT CTANAGCCTC TGCTAACCAT GTTCATGCCT TCTTCTTTTT   121 CCTACAGCTC CTGGGCAACG TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT   181 GATTTGATAC GTACGGTTCG GATCGATATC TGCGGCCGCA TGGAGCAACT ATCCGATGAA   241 GAGATTGACC ATGGTGCTGA AGAAGACAGC GACAAGGAAG ATCAGGACCT GGATAAAATG   301 TTTGGGGCCT GGCTTGGAGA ACTGGACAGG CTTACTCAGA GTTTGGATTC TGACAAGCCC   361 AAAGAACCNG TAAAAAGATC TCCTCTTCGC CAGGAAACAA ATATGGCCAA CTTTTCTTAC   421 CGCTTCTCCN TATACAACTT GAATGNAAGC TCTGNANTCA GGGTGAGACT GTGGACCTGG   481 ATGCCCTGAT GGCTGACCTC TGCTCTATNN ANNANGAGCT CAGCAGCNNT TGGCTCNNGA   541 AATAGTAANC GTCNAGNNNC AGANCCAAAN GTTCCTCANA NNCTNNCNNG NTGGCCNANA   601 TACNTCAAAA NNTNGNNNNC CNTGANAGNA NNNNCNTCTN NNNCNNNNNN NANNNACTAA   661 NNCTTNCCNN GNNNNNNANN NNCCNGNNNN ANATNNNNGN NNNNNNANAN NNNNNNNTNN 721 NTNNNNNNNN NN //
Primer: IRES-R(DEP1835) (Pr1303) Region: insert 3'(6xHis,mLpd 3') Sequence file: RDB13050_A5A5b.seq
>13050_13050_A5A5_IRES_R(DEP1835)_3_E03_15.ab1     1 NNNNNNNNCN NNNNTGAGTC GNANTACNAT TNNCTGGCCG NCNNTTTACN ACGTCGTGAC    61 TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGA   121 CTCAGTGGTG GTGGTGGTGG TGGTCGACCC AGTCTCTTGA GAGCTTAGTA TTCTGGTCTC   181 TCTTAGGAGG AGCGGGAGGG GGTCCTCTTC GCAATGTTGC ATAGCCCGAA ATATGACTAC   241 CTCCAAAGCC AGCCTTCTGC TGGTCAGACA GCAGTTCTGG TGGTGGTGGG GGTAGTGCCA   301 TGTCGTCCAT TGTGGCGGTG GGTTCTGCTC TGGACATACG TGAGGAGGAC AGGGAGCCAT   361 GCCGCGTGAT AGACTTGCGC TGCAATGTCC TGTTAAGATC AGCTAGGAAT CCAGGTTGGA   421 CGCTAAGGCT GGATTTAGGA GAGGTGGGCA CTTGTGGCAC AACTGTGGTC ATTGCTGGCT   481 GTTCAGATAT GTCAGCTTGA GTGAGGCGGG TGCTGTCATT GCGTTTGGGC CGGGTAGGTG   541 GGGGGGCCTT CTTCACTGAT GTTTTAGACC ATAGCTGTGG CTGAGGATTA ACAACTGCCA   601 CTTTGGGGGA GGTGTTCCCC GAGAATACAG CTGGAATCTC AATAGGGGGC AGANGAAGCT   661 CTATTTCAGG TGGAGGGGGT GGAAAGTCAG AATCAGATGG TGGCGATGGA AATTCCACTA   721 CAACGTCCTT TCCNCGCCCA CTCAGAACAG AGGCTTTTGC CGACACAATC CCTTGCTGGT   781 GGANTCCAGG GAGATTGACT CCAGAAAGGT GTANTTTCCC AGGTTTGGGG GGGTGCTGCA   841 NGANGNGGNG NNGTCNNTTT GCTGGGAGAN CCNGATTCTG NTGNNGGNGC NAANTTGCTG   901 ANTANACTGT CTANNNNNGG NCGCTTGGGC TCNNGNTGNN CTNNNCANCT GNNNNNANTT   961 ANNGNTNGNA NTNNNNCTNC NGGNNNGGGG GNNNNNTNNT TNCCCCNNNG GNNNNNNNNN 1021 N //
Primer: IRES-F (Pr0036) Region: IRES,S-Tag,Thrombin site,PuroR Sequence file: RDB13050_A5A5c.seq
>13050_13050_A5A5_IRES_F_3_E03_15.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNCGNNGTN NNNNNACGTC TANNNNNNNN CCNNNNNNNN    61 GTGGTTTTCC NNNNNNNNNN NNNNNNNAAT ATGGCCACCA CCCATATGAA AGAAACCGCT   121 GCTGCTAAAT TCGAACGCCA GCACATGGAC AGCCCAGATC AGGGTACCCT GGTGCCACGC   181 GGTTCCATGG CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCAGG   241 GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAT   301 CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG   361 CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG   421 CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG   481 AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC   541 AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT   601 CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC   661 TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC   721 ACCGCCGACG TCGAGGTGCC CGAAGGANCG CGCACCTGGT GCATGACCCG CAAGCCCGGT   781 GCCTGANTNN ACTTGTTTAT TGCAGCTTAT AATGGTTACN AATAAAGCAA TAGCATCACA   841 AATTTCNCAA ATAAAGCATT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT   901 NNATGTATCT TATCATGTCT GGGATCTCNN NNNNNNNGGG GGGGCCCGGT NCCCANCTTT   961 TGTTCCCTTT AGTGNNGGNN NNNNNGNNCG CTNNNNNNNA ANCNTGGGNN ATAGCTGNTT 1021 NCCNGNGNGA AANNNNNTNN NNCNNNNNNN NANTNNCNNN NNNNNNN //
Primer: PuroR_F (Pr0554) Region: PuroR,SV40 pA,lacO,lac pro Sequence file: RDB13050_A5A5d.seq
>13050_13050_B1JfF_3_PuroR_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNCGCGN NNNNNNNGNA TGACCCNNNN    61 NNCCGGTGCC TGAGTTAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG   121 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA   181 ACTCATCAAT GTATCTTATC ATGTCTGGAT CTCGACCTCG AGGGGGGGCC CGGTACCCAG   241 CTTTTGTTCC CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGT CATAGCTGTT   301 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GGAGCATAAA   361 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT   421 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG NNNNNCGCGC   481 GGGGAGAGGC GGTTTGCGTA TTGGGCGNNN NNNCGCTTCC TCGCTCACTG ACTCGCTGCG   541 CTCGGTCGTT CGGCTGCNNN NNNCGGNATC NGCTCACTCN AAGGCGGNNA TACNGTTATC   601 CACNNAATCN NGNNNNNACG CNNNAANAAC ATGNNAGCAA ANNNNNNNNN AAGNNNAGGA 661 ACCGTANNNA GGCCGCGTTG CTGGNNNTTT NNNNNNNGNN NCNNCCCCCN NNNNNNNNN //
Primer: pAxCA_F1 (Pr0164) Region: insert 5'(mLpd 5') Sequence file: RDB13050_A5A5e.seq
>13050_13050_A5A5_pAxCA_F1_3_C03_09.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNCTNCTN NTTNNNNNNC NNNNNNNNNN    61 NNNNNGCNGG NTATTGTGCT GNNNNNNNNN NNNNNNAANA ATTGATTTGA TACGTACGGT   121 TCGGATCGAT ATCTGCGGCC GCATGGAGCA ACTATCCGAT GAAGAGATTG ACCATGGTGC   181 TGAAGAAGAC AGCGACAAGG AAGATCAGGA CCTGGATAAA ATGTTTGGGG CCTGGCTTGG   241 AGAACTGGAC AGGCTTACTC AGAGTTTGGA TTCTGACAAG CCCAAAGAAC CAGTAAAAAG   301 ATCTCCTCTT CGCCAGGAAA CAAATATGGC CAACTTTTCT TACCGCTTCT CCATATACAA   361 CTTGAATGAA GCTCTGAATC AGGGTGAGAC TGTGGACCTG GATGCCCTGA TGGCTGACCT   421 CTGCTCTATA GAGCAGGAGC TCAGCAGCAT TGGCTCAGGA AATAGTAAGC GTCAAGTCAC   481 AGAACCAAAA GTTCCTCAGA AGCTACCTGC TGGCCGACAT ACGTCAAAAC ATGGCACCCT   541 GAGAGGACCG TCATCTTCCT CTAATAGAAC AACTAAACCT TCCCATGCTA ACTACTCCCT   601 GGATGACATC ACTGCTCAGT TAGAACAGGC TTCCTTGAGC ATGGATGAGG CTGCTCAGCA   661 ATCTCTGGTA GAAGATCCTA AGCCTTTAGT GACAAATCAG CACAGAAGGA CTGCATCAGC   721 AGGCACTGTG AGTGATGCTG AAGCGCGCTC CATCAGTAAC TCCTCCCGGT CAAGCATCAC   781 CTCTGCAGCC TCCAGCATGG ATTCTCTGGA TATTGATAAA GTGACACGCC CTCAAGAGCT   841 TGATTTGACC ACACACCANG GGCAGNNAAT TACTGAGCAT GCTATCTCTC TGANCTGTCN   901 NNNTAAGCNN GNNNNGCNNC ANTCTGACTT CNCNGAAGAN CNNGNTGANC TGACACCTNN   961 CTCCTATCTN GNACAGGNNN ANCNTNCCTT CNTNNTGANN NNNNNTGNNN NAAANCNTNC 1021 CNNTTTTGCN NNNNNNNNN //
Primer: PuroR-rev (Pr0423) Region: S-Tag,IRES Sequence file: RDB13050_A5A5f.seq
>13050_13050_B3C1F_3_PuroR-rev_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNG NNNNCCTGNN CTGGGCTGTC NNTGGTNNCT GGCGTTCGAA    61 TTTAGCAGCA GCGGTTTCTT TCATATGGGT GGTGGCCATA TTATCATCGT GTTTTTCAAA   121 GGAAAACCAC GTCCCCGTGG TTCGGGGGGC CTAGACGTTT TTTTAACCTC GACTAAACAC   181 ATGTAAAGCA TGTGCACCGA GGCCCCAGAT CAGATCCCAT ACAATGGGGT ACCTTCTGGG   241 CATCCTTCAG CCCCTTGTTG AATACGCTTG AGGAGAGCCA TTTGACTCTT TCCACAACTA   301 TCCAACTCAC AACGTGGCAC TGGGGTTGTG CCGCCTTTGC AGGTGTATCT TATACACGTG   361 GCTTTTGGCC GCAGAGGCAC CTGTCGCCAG GTGGGGGGTT CCGCTGCCTG CAAAGGGTCG   421 CTACAGACGT TGTTTGTCTT CAAGAAGCTT CCAGAGGAAC TGCTTCCTTC ACGACATTCA   481 ACAGACCTTG CATTCCTTTG GCGAGAGGGG AAAGACCCCT AGGAATGCTC GTCAAGAAGA   541 CAGGGCCAGG TTTCCGGGCC CTCACATTGC CAAAAGACGG CAATATGGTG GAAAATAACC   601 GGAATTAATT CGCCCTATAG TGAGTCGTAT TACAATTCAC TGGCCGTCGT TTTACAACGT   661 CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC TTGCAGCACA TCCCCCTTTC   721 GCCAGACTCN NTGNTGGTGG TGGTGGTGGN CGNNCNNNTC TCTTGAGAGC TTANTATTCN   781 GGTCTCTCTT ANNANGANCG GGAGGGGGTC CTCTTCNCAN NGNTGCATAN CCCGAAATAT   841 GACTANCNNC AAANCNANNC TTCNGCTGGN CANANNGCNN TNCTGGNGGN GGNGGGGGTN   901 NNNNNNTGNC NTCCATNGNN GNNNNGGNNN CTGNNNNNGA NNTNCNNNAG NNNNNNNNGN 961 NNNCNNNNNN NNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro,f1 ori Sequence file: RDB13050_A5A5g.seq
>13050_13050_B3C1F_3_Amp_R_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTTG NNNCNTTTAT NNGGGTTATT GTCTCATGAN CGGATACATA    61 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG   121 CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA   181 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA   241 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG   301 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACTA CGTGAACCAT   361 CACCCTAATC AAGTTTTTTG GGGTCGAGGT GCCGTAAAGC ACTAAATCGG AACCCTAAAG   421 GGAGCCCCCG ATTTAGAGCT TGACGGGGAA AGCCGGCGAA CGTGGCGAGA AAGGAAGGGA   481 AGAAAGCGAA AGGAGCGGGC GCTAGGGCGC TGGCAAGTGT AGCGGTCACG CTGCGCGTAA   541 CCACCACACC CGCCGCGCTT AATGCGCCGC TACAGGGCGC GTCCCATTCG CCATTCAGGC   601 TGCGCAACTG TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCTGGCGA   661 AAGGGGGATG TGCTGCAAGG CGATTAAGTT GGGTAACGCC AGGGTTTTCC CAGTCACGAC   721 GTTGTAAAAC GACGGCCAGT GAGCGCGCGT ANTACGACTC ACTATAGGGC GAATTGNANC   781 TCCNCGGANT CTAGTTATTA ATAGTAATCN ATTACNGGGN CATTAGTTCA TANCCNATAT   841 ATGGANTTCC GCGTTACATA ACTTANNGNA AATGNNCCGC CNGGNNGANC GCCNNACNAN   901 CCCGCCNATN GACNTCNATA NTGANGNANG NNCCCNTANN ANCNNNNNNA NGNANTTTNN 961 NTNGNNNTNN NNGNNNNNNN NNTNNNNNNN ANNNNNCNNN NNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl