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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pHTB Cas9

In vitro production of Cas9 mRNA.

Catalog number RDB12994
Resource name pHTB Cas9
Clone info. In vitro production of Cas9 mRNA.
Comment Linearize this plasmid with NotI and synthesize mRNA by utilizing T3 promoter.
Vector backbone pBS-HTB/N (Plasmid)
Size of vector backbone 3.2 kb
Selectable markers Amp^r
Gene/insert name Streptococcus pyogenes Cas9 genomic DNA
Depositor|Developer Sasakura, Yasunori |
Other clones in our bank
External Database
Streptococcus pyogenes Cas9
          Reference sequence
            
           
          Sequence (full) RDB12994zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Dev Growth Differ., 56, 499-510(2014)). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Dev Growth Differ., 56, 499-510(2014))。 利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB12994 pHTB Cas9 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pHTB Cas9 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12994).

          Reference section:

          Sasaki, H., Yoshida, K., Hozumi, A., Sasakura, Y., CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis. Dev. Growth Differ. 56 (7): 499-510 (2014). PMID 25212715.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Genome Editing (English text)
          Featured content Genome Editing (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12994_A4Jnp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: reverse-2 (Pr0100)
          Region: insert 5'
          Sequence file: RDB12994_A4Jna.seq check
          >RDB12994_3_reverse2_A06_03.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNNN NNNNNNCTCN CTAAAGGGAA CNAAAGCTGG
             61 AGCTCCNCAA TTCGGCAGAG TTAAGACCAG ATTCTTAGTT TTAGTTGAAG TGACTGGACG
            121 CGTACTGTCT GTGTCCGGCT TTTTTCTTTA TCTCACAGCC AAATCACTAA ATATCACCGG
            181 ATCCGATAAT GGATAAGAAA TACTCAATAG GCTTAGATAT CGGCACAAAT AGCGTCGGAT
            241 GGGCGGTGAT CACTGATGAA TATAAGGTTC CGTCTAAAAA GTTCAAGGTT CTGGGAAATA
            301 CAGACCGCCA CAGTATCAAA AAAAATCTTA TAGGGGCTCT TTTATTTGAC AGTGGAGAGA
            361 CAGCGGAAGC GACTCGTCTC AAACGGACAG CTCGTAGAAG GTATACACGT CGGAAGAATC
            421 GTATTTGTTA TCTACAGGAG ATTTTTTCAA ATGAGATGGC GAAAGTAGAT GATAGTTTCT
            481 TTCATCGACT TGAAGAGTCT TTTTTGGTGG AAGAAGACAA GAAGCATGAA CGTCATCCTA
            541 TTTTTGGAAA TATAGTAGAT GAAGTTGCTT ATCATGAGAA ATATCCAACT ATCTATCATC
            601 TGCGAAAAAA ATTGGTAGAT TCTACTGATA AAGCGGATTT GCGCTTAATC TATTTGGCCT
            661 TAGCGCATAT GATTAAGTTT CGTGGTCATT TTTTGATTGA GGGAGATTTA AATCCTGATA
            721 ATAGTGATGT GGACAAACTA TTTATCNNGT TGGTACNAAC CTACAATCAA TTATTTGAAG
            781 AAAACCCTAT TAACGCAAGT GGAGTAGATG CTAAAGCGAT TCTTTCTGCN CGATTGANTA
            841 AATCAAGACG ANTAGAAAAA TCTCATTGCT CANCTCCCCG GTGNNAANAA AAANTGGCTN
            901 ATTTGGGANT CTCATTGCTT TGNNNATNGG GTTNGACCCC TAATTTTAAA NCAAATTTTT
            961 GATTTGGCNG ANNNNGCTNA NNTNNCNNCT TTNNAAANNA TACTTNCNAT GANGATTNNA
          1021 TNANTTTNNN NNNNGNNNAA NNNNNNANNN NNNNNNN
          //
          Primer: cas9-B_F (Pr1256)
          Region: insert 3',SV40 NLS,T7 pro
          Sequence file: RDB12994_A4Jnb.seq check
          >12994_12994_B2JeF_3_cas9-B_F_F01_06_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNAGCTGGC TCTGCNNAGC AAATATGTGA ATTTTTTATA
             61 TTTAGCTAGT CATTATGAAA AGTTGAAGGG TAGTCCAGAA GATAACGAAC AAAAACAATT
            121 GTTTGTGGAG CAGCATAAGC ATTATTTAGA TGAGATTATT GAGCAAATCA GTGAATTTTC
            181 TAAGCGTGTT ATTTTAGCAG ATGCCAATTT AGATAAAGTT CTTAGTGCAT ATAACAAACA
            241 TAGAGACAAA CCAATACGTG AACAAGCAGA AAATATTATT CATTTATTTA CGTTGACGAA
            301 TCTTGGAGCT CCCGCTGCTT TTAAATATTT TGATACAACA ATTGATCGTA AACGATATAC
            361 GTCTACAAAA GAAGTTTTAG ATGCCACTCT TATCCATCAA TCCATCACTG GTCTTTATGA
            421 AACACGCATT GATTTGAGTC AGCTAGGAGG TGACGGTTCT CCCAAGAAGA AGAGGAAAGT
            481 CTCGAGCGGT GGAGCTGCAG GATGAAATTC GATATCTAAC CGACTCCACT CCCACACAAT
            541 TAAGACAATG CTTGGTGATT TGGACTTTTG GCTAAATTAT ATAATAAAAA TTTGAAAAGG
            601 TTGATATAAT AAACTAATTT GAAAAGCTAA AAAAAAAAAA AAAAAACTCG AGGCGGCCGC
            661 GGTACCCAAT TCGCCCTATA GTGAGTCGTA TTACAATTCA CTGGCCGTCG TTTTACAACG
            721 TCGTGNCTGG GAAAACCCTG NCGTTACCCA ACTTAATCGC CTTGCAGCAC ATCCCCCTTT
            781 CGCCAGCTGG CGTANTANCG AANAGGCCCG CACCGATCGC CCTTCCCAAC AGTTGCGCAG
            841 CCNGAATGGN NNANGNNAAN NTGTANNCGT NAATANTTTG TTAAAATTCN CGNNNAATTT
            901 TTGNNAANTC ANCTCATTTT TTNNNNNNNA NGNNNNANNN NGGNNAAANN NNNANNANNC
          961 AAANNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Sasaki, H., CRISPR/Cas9-mediated gene knockout in the ascidian Ciona intestinalis. Dev. Growth Differ. 56 (7): 499-510 (2014). PMID 25212715.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl