Please review the QC test results indicated by icon below as well as clone information before placing your order.

CS-hp53-shRNA-CG

Lentivirus vector plasmid of shRNA targeting human TP53 with GFP marker.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	((Remarks)) Remember that you will be working with samples containing infectious virus. Please contact us. We will provide you with appropriate documents.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12946_A4K5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: cPPT+CTS_v2_F Sequence file: RDB12946_A4K5a.seq
>12946_12946_A4K5_2_cPPT+CTS_v2_F_B09_06.ab1     1 TCCTAGGAAA TCGGACTAAA GAATTACAAA AACAAATTAC AAAAAATTCA AAATTTTCGG    61 GTTTATTACA GGGACAGCAG AAATTCACTT TGAATTATCA CAAGTTTGTA CAAAAAAGCA   121 GGCTCCACCA TGGGAACCAA TTCAGTCGAC TGGATCCGGT ACCGAATTCG AACGCTGACG   181 TCATCAACCC GCTCCAAGGA ATCGCGGGCC CAGTGTCACT AGGCGGGAAC ACCCAGCGCG   241 CGTGCGCCCT GGCAGGAAGA TGGCTGTGAG GGACAGGGGA GTGGCGCCCT GCAATATTTG   301 CATGTCGCTA TGTGTTCTGG GAAATCACCA TAAACGTGAA ATGTCTTTGG ATTTGGGAAT   361 CTTATAAGTC CCTATCAGTG ATAGAGATCC CCACTCTAGT GGTGATTTAC TACGTGTGCT   421 GTCCGTAGTA GATTACCACT GGAGTTTTTT GGAAATCTAG ACCCAGCTTT CTTGTACAAA   481 GTGGTGATAA TTCGATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA GCCCATATAT   541 GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC CCAACGACCC   601 CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG GGACTTTCCA   661 TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC TTGGCAGTAC ATCAAGTGTA   721 TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG CCTGGCATTA   781 TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG TATTAGTCAT   841 CGCTATTACC ATGGTGATGC GGTTTTGGCA GTACATCAAT GGGCGTGGAT AGCGGTTTGA   901 CTCACGGGGA TTTCCAAGTC TCCACCCCAT TGACGTCAAT GGGAGTTTGT TTTGGCACCA   961 AAATCAACGG GACTTTCCAA AAATGTCGTA ACAACTCCGC CCCAATTGAC GCAAAATGGG  1021 CGGTAGGCGT GTACGGGTGG GGAGGTCTAA TATAAAGCAG AGCTGGGTTT TAGTGAAACG  1081 TTCAGATCCG CTAGCGCTTA CGATTCGCAT CATGATTGAG CCAAGGACGG AGGAAGCTGT 1141 TTCAACCCGG GGGGTTGACG //
Primer: PRE_rev Sequence file: RDB12946_A4K5b.seq
>12946_12946_A4K5_2_PRE_rev_C09_09.ab1     1 CGGGATTCAA ATAGCGTAAA GGAGCACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT    61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGAGATCCTA CTAGTAGGAT   121 CTGAGTCCGG ACTTGTACAG CTCGTCCATG CCGAGAGTGA TCCCGGCGGC GGTCACGAAC   181 TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT TGCTCAGGGC GGACTGGGTG   241 CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC CGATGGGGGT GTTCTGCTGG   301 TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT GGCGGATCTT GAAGTTCACC   361 TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT TGTGGCTGTT GTAGTTGTAC   421 TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT CGATGCCCTT CAGCTCGATG   481 CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC GGGTCTTGTA GTTGCCGTCG   541 TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG GCATGGCGGA CTTGAAGAAG   601 TCGTGCTGCT TCATGTGGTC GGGGTAGCGG CTGAAGCACT GCACGCCGTA GGTCAGGGTG   661 GTCACGAGGG TGGGCCAGGG CACGGGCAGC TTGCCGGTGG TGCAGATGAA CTTCAGGGTC   721 AGCTTGCCGT AGGTGGCATC GCCCTCGCCC TCGCCGGACA CGCTGAACTT GTGGCCGTTT   781 ACGTCGCCGT CCAGCTCGAC CAGGATGGGC ACCACCCCGG TGAACAGCTC CTCGCCCTTG   841 CTCACCATGG TGGCGACCGG TAGCGCTAGC GGATCTGACG GTTCACTAAA CCAGCTCTGC   901 TTATATAGAC CTCCCACCGT ACACGCCTAC CGCCCATTTG CGTCAATGGG GCGGGAGTTG   961 TTACGACATT TTGGGAAAGT CCCGTTTGAT TTTGGGTGCC CAAAACAAAC TCCCCATTGA  1021 CGTCATGGGG TGAGACTGTA ATCCCCCGTG AGTCAACCGC TATCCACGCC CAATGAATGT 1081 ACTGCCTAAG CGCATCACAT GATAATAGCG AATGAACTAA TTACGTAGAA ATGGTTAC //
Primer: HIVgp_R1 Sequence file: RDB12946_A4K5c.seq
>12946_12946_A4K5_HIVgp_R1_2_A01_01.ab1     1 CCGGCCTCCT ATTCCACTGC TCTTTTTTCT CTCTGCACCA CTCTTCTCTT TGCCTTGGTG    61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA   121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGCTTGCT GTGCGGTGGT   181 CTTACTTTTG TTTTGCTCTT CCTCTATCTT GTCTAAAGCT TCCTTGGTGT CTTTTATCTC   241 TATCCTTTGA TGCACACAAT AGAGGGTTGC TACTGTATTA TATAATGATC TAAGTTCTTC   301 TGATCCTGTC TGAAGGGATG GTTGTAGCTG TCCCAGTATT TGTCTACAGC CTTCTGATGT   361 TTCTAACAGG CCAGGATTAA CTGCGAATCG TTCTAGCTCC CTGCTTGCCC ATACTATATG   421 TTTTAATTTA TATTTTTTCT TTCCCCCTGG CCTTAACCGA ATTTTTTCCC ATCGCGATCT   481 AATTCTCCCC CGCTTAATAC TGACGCTCTC GCACCCATCT CTCTCCTTCT AGCCTCCGCT   541 AGTCAAAATT TTTGGCGTAC TCACCAGTCG CCGCCCCTCG CCTCTTGCCG TGCGCGCTTC   601 AGCAAGCCGA GTCCTGCGTC GAGAGAGCTC CTCTGGTTTC CCTTTCGCTT TCAAGTCCCT   661 GTTCGGGCGC CACTGCTAGA GATTTTCCAC ACTGACTAAA AGGGTCTGAG GGATCTCTAG   721 TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA GCACTCAAGG CAAGCTTTAT   781 TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT CCCAGGCTCA GATCTGGTCT   841 AACCAGAGAG ACCCAGTACA GGCAAAACGC GCTGCTTATA TAGACCTCCC ACCGTACACG   901 CCTACCGCCC ATTTGCGTCA ATGGGGGCGG AGTTGTTACG ACATTTTGGA AAGTCCCGTT   961 GATTTTGGGT GCCAAAACAA ACTCCCATTG ACGTCAATGG GGGTGGAGAC TTGGGAAATC  1021 CCCGTGAGTC AACCCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC ATCACCCATG  1081 GTAATAGCCG GATGACTATA CGGTAGATGT ACTGCCAAGG TAGAAAGTCC CATAAGTCAT 1141 GTACTTGGGC ATAATGCCAG GGCGGGGCCC AATTTTAACC CGGTTCCA //
Primer: BGH_rev2 Sequence file: RDB12946_A4K5d.seq
>12946_12946_A4K5_2_BGHrev2_D09_12.ab1     1 CATTTGGTTC AAGAGCACGG GGGAGGGGCA AACAACAGAT GGCTGGCAAC TAGAAGGCAC    61 AGTCGAGGCT GATCAGCGGG TTTAAACGGG CCCTGCTAGA GATTTTCCAC ACTGACTAAA   121 AGGGTCTGAG GGATCTCTAG TTACCAGAGT CACACAACAG ACGGGCACAC ACTACTTGAA   181 GCACTCAAGG CAAGCTTTAT TGAGGCTTAA GCAGTGGGTT CCCTAGTTAG CCAGAGAGCT   241 CCCAGGCTCA GATCTGGTCT AACCAGAGAG ACCCAGTACA GTCCGGATGC AGCTCTCGGG   301 CCATGTGATG AAATGCTAGG CGGCTGTCAA ACCTCCACTC TAATACTTCT CTCTCCGGGT   361 CATCCATCCC ATGCAGGCTC ACAGGGTGTA ACAAGCGGGT GTTCTCTCCT TCATTGGCTT   421 CTTCTACCTT CTCTTGCTCA ACTGGTACTA GCTTGTAGCA CCATCCAAAG GTCAGTGGAT   481 ATCTGATCCC TGGCCCTGGT GTGTAGTTCT GCCAATCAGG GAAGTAGCCT TGTGTGTGGT   541 AGATCCACAG ATCAAGGATA TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC   601 CCTTTTCTTT TAAAAAGTGG CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG   661 GTACCTGAGG TGTGACTGGA AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC   721 AATCAGCATT GGTAGCTGCT GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC   781 GACGGTATCG ATGCGGGGAG GCGGCCCAAA GGGAGATCCG ACTCGTCTGA GGGCGAAGGC   841 GAAGACGCGG AAGAGGCCGC AGAGCCGGCA GCAGGCCGCG GGAAGGAAAG GTCCGCTGGA   901 TTGAGGGCCG AAAGGGACGT AGCAGAACGA CGTCCCGCGC AGAATCCAGG TGGCAACACA   961 GGCGAGCAGC CATGGGAAAG GGACGTCAGC TTCCCCGACA CACCCACGGG AATGTCAGTG  1021 CCCACAGCCG AGCCCCTGTC AGCAGCGGGC AAGCAGGCGC CGATGAGTCG GCGTGTCATT 1081 AGAGGGGGAA AGCGAAGTTC CTGCAAGGAG CTGAACAAGG TTGGGGTGGG CCA //
Primer: SV40pro_F Sequence file: RDB12946_A4K5e.seq
>RDB12946_12946_A4K5_SV40pro_F_2_H12_24.ab1     1 CCCTGTCCTT GCTTTCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA    61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA GCACGTGTTG ACAATTAATC   121 ATCGGCATAG TATATCGGCA TAGTATAATA CGACAAGGTG AGGAACTAAA CCATGGCCAA   181 GTTGACCAGT GCCGTTCCGG TGCTCACCGC GCGCGACGTC GCCGGAGCGG TCGAGTTCTG   241 GACCGACCGG CTCGGGTTCT CCCGGGACTT CGTGGAGGAC GACTTCGCCG GTGTGGTCCG   301 GGACGACGTG ACCCTGTTCA TCAGCGCGGT CCAGGACCAG GTGGTGCCGG ACAACACCCT   361 GGCCTGGGTG TGGGTGCGCG GCCTGGACGA GCTGTACGCC GAGTGGTCGG AGGTCGTGTC   421 CACGAACTTC CGGGACGCCT CCGGGCCGGC CATGACCGAG ATCGGCGAGC AGCCGTGGGG   481 GCGGGAGTTC GCCCTGCGCG ACCCGGCCGG CAACTGCGTG CACTTCGTGG CCGAGGAGCA   541 GGACTGACAC GTGCTACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAA GGTTGGGCTT   601 CGGAATCGTT TTCCGGGACG CCGGCTGGAT GATCCTCCAG CGCGGGGATC TCATGCTGGA   661 GTTCTTCGCC CACCCCAACT TGTTTATTGC AGCTTATAAT GGTTACAAAT AAAGCAATAG   721 CATCACAAAT TTCACAAATA AAGCATTTTT TTCACTGCAT TCTAGTTGTG GTTTGTCCAA   781 ACTCATCAAT GTATCTTATC ATGTCTGTAT ACCGTCGACC TCTAGCTAGA GCTTGGCGTA   841 ATCATGGTCA TAGCTGTTTC CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT   901 ACGAGCCGGA AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT   961 AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC AGCTGCATTA  1021 ATGAATCGGC CAACGCGCGG GGAGAGGCGG GTTTGCGTAT TGGCGCTCTT CCGCTTTCCT  1081 CGCTCACTGA CTCGCTGCGC TCGGGTCGTT TCGGCTGCGG CGAGCGTATC AGCTCACTCA  1141 AAAGCGTATA CGGCTATCCA CAGGAATCAG GATTACGCGA AGCAAAGGAA ATCCAGTAGT 1201 CCGTGGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl