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CSIV-RfA-CG

Catalog number RDB12884
Resource name CSIV-RfA-CG
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone Plasmid
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Growth remarks Use DB3.1, ccdB Survival or equivalent to amplify this clone. 30 degC is fine.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB12884zzt01.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
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            MTA, for use for not-for-profit academic purpose)[Word]
            Please visit Ordering instruction.[link] 
            Remarks Remember that you will be working with samples containing infectious virus.
            Use Survival2 (Invitrogen) or equivalent to amplify this clone.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。
            このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

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            RDB12884 CSIV-RfA-CG DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CSIV-RfA-CG was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12884).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Lentiviral plasmid list (English text)
            Featured content Lentivirus Vector (English text)
            Featured content Lentivirus Vector (Japanese text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12884_B3Gep1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Cm_M_R (Pr0012)
            Region: CmR,attR1,luc UV50 pro,cPPT_CTS
            Sequence file: RDB12884_B3Gea.seq check
            >12884_12884_B3Ge_1_Cm_M_R_F02_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNCNNNT ATNNCNNGCT CNCCNNCTTN NNNTGCCATA CGGAATTCCG
               61 GATGNAGCAT TCATCAGGCG GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA
              121 TTTTTCTTTA CGGTCTTTAA AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA
              181 CATTGAGCAA CTGACTGAAA TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA
              241 ACGGTGGTAT ATCCAGTGAT TTTTTTCTCC ATTTTAGCTT CCTTAGCTCC TGAAAATCTC
              301 GACGGATCCT AACTCAAAAT CCACACATTA TACGAGCCGG AAGCATAAAG TGTAAAGCCT
              361 GGGGTGCCTA ATGCGGCCGC CATAGTGACT GGATATGTTG TGTTTTACAG TATTATGTAG
              421 TCTGTTTTTT ATGCAAAATC TAATTTAATA TATTGATATT TATATCATTT TACGTTTCTC
              481 GTTCAGCTTT TTTGTACAAA CTTGTGATAA TTCAAAGTGA ATTTCTGCTG TCCCTGTAAT
              541 AAACCCGAAA ATTTTGAATT TTTGTAATTT GTTTTTGTAA TTCTTTAGTT TGTATGTCTG
              601 TTGCTATTAT GTCTACTATT CTTTCCCCTG CACTGTACCC CCCAATCCCC CCTTTTCTTT
              661 TAAAATTGTG GNNNNANACT GNCNTTTGTA GATNCGTTNA CNNANTCNNN NNNNNNTGTC
            721 NCTNNCTCTC TCNNNNNNNT NNNNNNNNNN
            //
            Primer: ccdb-M_F (Pr0951)
            Region: ccdB,attR2,CMV en.2
            Sequence file: RDB12884_B3Geb.seq check
            >12884_12884_B3Ge_1_ccdb-M_F_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNGNNNNA NTGNNTGNNC TNNGCNNCCG CGNAAATGAC ATCAAAAACG
               61 CCATTAACCT GATGTTCTGG GGAATATAAA TGTCAGGCTC CCTTATACAC AGCCAGTCTG
              121 CAGGTCGACC ATAGTGACTG GATATGTTGT GTTTTACAGT ATTATGTAGT CTGTTTTTTA
              181 TGCAAAATCT AATTTAATAT ATTGATATTT ATATCATTTT ACGTTTCTCG TTCAGCTTTC
              241 TTGTACAAAG TGGTGATAAT TCGATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG
              301 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC
              361 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG
              421 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA
              481 TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC
              541 CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT ACTTGGCAGT ACATCTACGT
              601 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACATCAATG GGCGTGGATA
              661 GCGGTTTGAC TCACGGGGNN TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT
              721 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC AACTCCGCCC CATTGACGCA
              781 AATGNNCGGT ANGCGTGTAC GGNGGGNNGN CTATATAAGC AGANCTNGTT TANTGAANCG
              841 TCANANCCGC TANCGCTACN NNNCGCCACN NNGGNGANCA ANGGCNANNN NCNNNTCNNN
            901 NGGGNNNNNN NCNNTCCNNN NNNANNNNGN NNNGNNNNNN NNANNNNNNN NNN
            //
            Primer: PRE_rev (Pr0250)
            Region: PRE,EGFP 3'
            Sequence file: RDB12884_B3Gec.seq check
            >12884_12884_B3Ge_1_PRE_rev_D02_04_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNCAACATA GTTAAGAATA CCAGTCAATC TTTCACAAAT
               61 TTTGTAATCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGATGTTAAC TCTAGAGGCT
              121 CGAGATCCTA CTAGTAGGAT CTGAGTCCGG ACTTGTACAG CTCGTCCATG CCGAGAGTGA
              181 TCCCGGCGGC GGTCACGAAC TCCAGCAGGA CCATGTGATC GCGCTTCTCG TTGGGGTCTT
              241 TGCTCAGGGC GGACTGGGTG CTCAGGTAGT GGTTGTCGGG CAGCAGCACG GGGCCGTCGC
              301 CGATGGGGGT GTTCTGCTGG TAGTGGTCGG CGAGCTGCAC GCTGCCGTCC TCGATGTTGT
              361 GGCGGATCTT GAAGTTCACC TTGATGCCGT TCTTCTGCTT GTCGGCCATG ATATAGACGT
              421 TGTGGCTGTT GTAGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT
              481 CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC
              541 GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGANGGTGCG CTCCTGGACG TAGCCTTCGG
              601 GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG CTGAAGCACT
              661 GCACGCCGNN NNTCNNNNNG GTCACNANGG TGGGNCNGGG NNCNGNNNGN NTNNNNNNNN
            721 GNNNNNNNNN N
            //
            Primer: HIV-B_R (Pr0673)
            Region: 5' LTR,CMV pro.1,CMV en.1
            Sequence file: RDB12884_B3Ged.seq check
            >12884_12884_B3Ge_1_HIV-B_R_C02_08_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNAA NNTTTTGNNG TANTCNCCAG TCGCCGCCCC TCGCCTCTTG
               61 CCGTGCNNNN NNNNNNNNGC CGAGTCCTGC GTCGAGAGAG CTCCTCTGGN TNNNNTTTCG
              121 CTTTCAAGTC CCTGTTCGGG CGCCACTGCT AGAGATTTTC CACACTGACT AAAAGGGTCT
              181 GAGGGATCTC TAGTTACCAG AGTCACACAA CAGACGGGCA CACACTACTT GAAGCACTCA
              241 AGGCAAGCTT TATTGAGGCT TAAGCAGTGG GTTCCCTAGT TAGCCAGAGA GCTCCCAGGC
              301 TCAGATCTGG TCTAACCAGA GAGACCCAGT ACAGGCAAAA CGCGCTGCTT ATATAGACCT
              361 CCCACCGTAC ACGCCTACCG CCCATTTGCG TCAATGGGGC GGAGTTGTTA CGACATTTTG
              421 GAAAGTCCCG TTGATTTTGG TGCCAAAACA AACTCCCATT GACGTCAATG GGGTGGAGAC
              481 TTGGAAATCC CCGTGAGTCA AACCGCTATC CACGCCCATT GATGTACTGC CAAAACCGCA
              541 TCACCATGGT AATAGCGATG ACTAATACGT AGATGTACTG CCAAGTAGGA AAGTCCCATA
              601 AGGTCATGTA CTGGGCATAA TGCCAGGCGG GCCATTTACC GTCATTGACG TCAATAGGGG
              661 GCGTACTTGG CATATGATAC ACTTGATGTA CTGCCNAGTG GGCAGTTTAC CGTAAATACT
              721 CCACCCATTG ACGTCAATGG AAAGTCCCTA TTGNCGTTAC TATGGGAACA TACGTCATTA
              781 TTGACGTCNA TGNNNGGGGG TCGTTGGGCG GNCAGCCAGG CGGGCCATTT ANCGTAAGTT
              841 ATGTAACGCN GNANNTNNNT ATATNGNCTA TNANCTAATG ANCCCNNNAT TGANTNCTAT
              901 TNANNACTAN NCNATNANCN ANGNNNNNNN NNATATCTGG NCNGTNNNTC NNNNANNNNN
            961 NNNAANNNNN NNANNNNNNN NNN
            //
            Primer: EGFP-N_R2 (Pr0905)
            Region: EGFP 5',CMV pro.2,CMV en.2
            Sequence file: RDB12884_B3Gee.seq check
            >12884_12884_B3Ge_1_EGFP-N_R2_G02_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNGCNNNN CNNNNCNNNC CGNANGTCAG GGNGGTCACG
               61 AGGGTGGGCC AGGGNACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG
              121 CCGTAGGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG
              181 CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC
              241 ATGGTGGCGA CCGGTAGCGC TAGCGGATCT GACGGTTCAC TAAACCAGCT CTGCTTATAT
              301 AGACCTCCCA CCGTACACGC CTACCGCCCA TTTGCGTCAA TGGGGCGGAG TTGTTACGAC
              361 ATTTTGGAAA GTCCCGTTGA TTTTGGTGCC AAAACAAACT CCCATTGACG TCAATGGGGT
              421 GGAGACTTGG AAATCCCCGT GAGTCAAACC GCTATCCACG CCCATTGATG TACTGCCAAA
              481 ACCGCATCAC CATGGTAATA GCGATGACTA ATACGTAGAT GTACTGCCAA GTAGGAAAGT
              541 CCCATAAGGT CATGTACTGG GCATAATGCC AGGCGGGCCA TTTACCGTCA TTGACGTCAA
              601 TAGGGGGCGT ACTTGGCATA TGATACACTT GNTGTACTGC CNAGTGGGCA GTTTACCGTA
              661 AATACTCNAC CCATTGACGT CNATGNAAAG TCCCNTATTN NNNNTACTAN NGGNNNCNTA
            721 CNNNNNTNNN TNNNNNNNNN N
            //
            Primer: EGFP-M_R (Pr0743)
            Region: EGFP 5',CMV pro.2
            Sequence file: RDB12884_B3Gef.seq check
            >12884_12884_B3Ge_1_EGFP-M_R_H02_08_ABI08.ab1
                1 NNNNNNNNNN NNNNGNGGNG GNNCTTGNNN TTCNCCTTGA TGCCGTTCTT CTGCTTGTCG
               61 GCCATGATAT AGACGTTGTG GCTGTTGTAG TTGTACTCCA GCTTGTGCCC CAGGATGTTG
              121 CCGTCCTCCT TGAAGTCGAT GCCCTTCAGC TCGATGCGGT TCACCAGGGT GTCGCCCTCG
              181 AACTTCACCT CGGCGCGGGT CTTGTAGTTG CCGTCGTCCT TGAAGAAGAT GGTGCGCTCC
              241 TGGACGTAGC CTTCGGGCAT GGCGGACTTG AAGAAGTCGT GCTGCTTCAT GTGGTCGGGG
              301 TAGCGGCTGA AGCACTGCAC GCCGTAGGTC AGGGTGGTCA CGAGGGTGGG CCAGGGCACG
              361 GGCAGCTTGC CGGTGGTGCA GATGAACTTC AGGGTCAGCT TGCCGTAGGT GGCATCGCCC
              421 TCGCCCTCGC CGGACACGCT GAACTTGTGG CCGTTTACGT CGCCGTCCAG CTCGACCAGG
              481 ATGGGCACCA CCCCGGTGAA CAGCTCCTCG CCCTTGCTCA CCATGGTGGC GACCGGTAGC
              541 GCTAGCGGAT CTGACGGTTC ACTAAACCAG CTCTGCTTAT ATAGACCTCC CACCGTACAC
              601 GCCTACCGCC CATTTGCGTC AATGGGGCGG ANTTGTTACG ACATTTTGGA AAGTNCCGTT
            661 GNNTTTGGTG NCNAAACAAA CTCCCATTGN CGTCNANNGG GGNNNNNNNN NN
            //
            Primer: bGH_rev3 (Pr0885)
            Region: 3' LTR,loxP,PRE
            Sequence file: RDB12884_B3Geg.seq check
            >12884_12884_B3Ge_1_bGH_rev3_F01_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN NGGGCNNNNA ACAGNNGGCT GGCAACTAGA AGGCACAGTC
               61 GAGGCTGATC NNNNNNTTTA AACGGGCCCT GCTAGAGATT TTCCACACTG ACTAAAAGGG
              121 TCTGAGGGAT CTCTAGTTAC CAGAGTCACA CAACAGACGG GCACACACTA CTTGAAGCAC
              181 TCAAGGCAAG CTTTATTGAG GCTTAAGCAG TGGGTTCCCT AGTTAGCCAG AGAGCTCCCA
              241 GGCTCAGATC TGGTCTAACC AGAGAGACCC AGTACAGTCC GGAATAACTT CGTATAATGT
              301 ATGCTATACG AAGTTATGAT ATCTTGTCTT CGTTGGGAGT GAATTAGCCC TTCCAGTCCC
              361 CCCTTTTCTT TTAAAAAGTG GCTAAGATCT ACAGCTGCCT TGTAAGTCAT TGGTCTTAAA
              421 GGTACCTGAG GTGTGACTGG AAAACCCACC TCCTCCTCCT CTTGTGCTTC TAGCCAGGCA
              481 CAATCAGCAT TGGTAGCTGC TGTATTGCTA CTTGTGATTG CTCCATGTTT TTCTAGGTCT
              541 CGACGGTATC GATGCGGGGA GGCGGCCCAA AGGGAGATCC GACTCGTCTG AGGGCGAAGG
              601 CGAAGACGCG GAAGAGGCCG CAGAGCCGGC AGCAGGCCGC GGGAAGGAAG GTCCGCTGGA
              661 TTGAGGGCCG AANGGACGTA NCAGAANGAC GTCCCGCGCA GAATCCAGGT GGCAACACAG
              721 GCGANCAGCC ATGGAAAGGA CGTCAGCTTC CCCGACAACA CCACGGAATT GTCAGTGCCC
              781 AACAGCCGAN CCCCTGTCCA GCANCGGGCA AGGCANGCGG CGATGANTTC CGCNNNNNCN
              841 ATANGGANGG GGGAAAGCGA ANNTCCNNGA AANGANCTGA NANGNNGNGG CAANGNCCNN
              901 NCCAGNGGGG GGTTGCGNCN NCNAACACNG NGCNNNNNAN NNCNNNNNTN NNCNNNNNNN
            961 NNGGNNNNNN NNNN
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: Zeo,SV40 pA
            Sequence file: RDB12884_B3Geh.seq check
            >12884_12884_B3Ge_1_SV40pro_ori_F_G02_20_ABI24.ab1
                1 NNNNNNNNNN NNNNNGCCNC CTCTGCCTCT GANCTATTCN NGAAGTAGTG AGGAGGCTTT
               61 TTTGGAGNNN NNNNNNNTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT NNNNNNATCT
              121 GATCAGCACG TGTTGACAAT TAATCATCGG CATAGTATAT CGGCATAGTA TAATACGACA
              181 AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT TCCGGTGCTC ACCGCGCGCG
              241 ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG GTTCTCCCGG GACTTCGTGG
              301 AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT GTTCATCAGC GCGGTCCAGG
              361 ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT GCGCGGCCTG GACGAGCTGT
              421 ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA CGCCTCCGGG CCGGCCATGA
              481 CCGAGATCGG CGAGCAGCCG TGGGGGCGGG AGTTCGCCCT GCGCGACCCG GCCGGCAACT
              541 GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTGCT ACGAGATTTC GATTCCACCG
              601 CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA TCGTTTTCCG GGACGCCGGC TGGATGATCC
              661 TCCAGCGCGG GGATCTCATG CTGGAGTTCT TCGCCCACCC CAACTTGTTT ATTGCAGCTT
              721 ATAATGNTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA TTTTTTTCAC
              781 TGCATTCTAN TTGTGGTTTG TCCNAACTCA TCAATGTATC TTATCATGTC TGTATACCGT
              841 CNANCTCTAN CTAGANCTTG GCGTAATCAT GGNCATANCN GNTNCCTGNG NGAAATTGTT
              901 NTCCNCTCNC NATTCCNCNN NNNATACNAN CCNGANNNAT AAANNGNAAA GNCNNGGGNN
            961 GNCNNNNNNN NNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2024.03.26

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