Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSIV-RfA

Backbone vector plasmid of lentivirus construct.

Sequence (full)	RDB12882zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12882_B0Jkp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Cm_M_R Region: CmR, lacUV5 pro, attR1, cPPT_CTS Sequence file: RDB12882_B0Jka.seq
>12882_12882_B0Jk_2_Cm-M_R_F02_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNATNNCNNG CTCNCCGTCT TTNNTTGCCN TACGGAATTC    61 CGGATGAGCA TTCATCAGGC GGGCAAGAAT GTGAATAAAG GCCGGATAAA ACTTGTGCTT   121 ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT AATATCCAGC TGAACGGTCT GGTTATAGGT   181 ACATTGAGCA ACTGACTGAA ATGCCTCAAA ATGTTCTTTA CGATGCCATT GGGATATATC   241 AACGGTGGTA TATCCAGTGA TTTTTTTCTC CATTTTAGCT TCCTTAGCTC CTGAAAATCT   301 CGACGGATCC TAACTCAAAA TCCACACATT ATACGAGCCG GAAGCATAAA GTGTAAAGCC   361 TGGGGTGCCT AATGCGGCCG CCATAGTGAC TGGATATGTT GTGTTTTACA GTATTATGTA   421 GTCTGTTTTT TATGCAAAAT CTAATTTAAT ATATTGATAT TTATATCATT TTACGTTTCT   481 CGTTCAGCTT TTTTGTACAA ACTTGTGATA ATTCAAAGTG AATTTCTGCT GTCCCTGTAA   541 TAAACCCGAA AATTTTGAAT TTTTGTAATT TGTTTTTGTA ATTCTTTAGT TTGTATGTCT   601 GTTGCTATTA TGTCTACTAT TCTTTCCCCT GCACTGTACC CCCCCNATCC CCCCTTTTCN   661 TTNAAAANTG NGGNNNAANA CTGNCATTTG TANANCCNNN CNCTAATCNN ANNNNNNNNG 721 NNNNNGNNNN NNNNNN //
Primer: ccdB-C_F Region: ccdB, attR2, PRE Sequence file: RDB12882_B0Jkb.seq
>12882_12882_B0Jk_ccdB-C_F_E08_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGNNNNNNN NTGGCGCATG ATGACCACCG ATATGGCCAG    61 TGTGCCGGTC TCCGTTATCG GGGAAGAAGT GGCTGATCTC AGCCACCGCG AAAATGACAT   121 CAAAAACGCC ATTAACCTGA TGTTCTGGGG AATATAAATG TCAGGCTCCC TTATACACAG   181 CCAGTCTGCA GGTCGACCAT AGTGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT   241 GTTTTTTATG CAAAATCTAA TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT   301 CAGCTTTCTT GTACAAAGTG GTGATGCTAC CGGTCTCGAG CCTCTAGAGT TAACATCGAG   361 GGATCAAGCT TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA   421 TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC   481 ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT   541 CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG   601 CTGACNCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG TCNNCTCCTT TCCGGGACTT   661 TCGCTTTCCC CNTCCCNATT GNNNCNNNNN NNNCATCNNN NNNNGNCNTN NNCCNCTGCT 721 NNNNNNNNNN //
Primer: CMV_Forward Region: CMV pro, 5' LTR, HIV-1 psi Sequence file: RDB12882_B0Jkc.seq
>12882_12882_B0Jk_CMV-Forward_C08_08_ABI24.ab1     1 NNNNNNNNNN NNNNNTGNNN NNNTGGGCGG TAGGCGTGNN NNGTGGGAGG TCTATATAAG    61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT   121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG   181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT   241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC   301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG   361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG   421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT   481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT   541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT   601 GGGNCAGCTA CAACCATCCC TTCNGACNGG ATCNGAAGAN CTTANATCNT TATATAATAC 661 NGTANCNACC CTCTANNGNG NGNNTCNNNG ATANNNNNAA ANNNNNNNNN N //
Primer: RRE_F1 Region: RRE, gp41 peptide Sequence file: RDB12882_B0Jkd.seq
>12882_12882_B0Jk_RRE_F1_D08_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN ANNNTTTGCT GANGGCTATT GAGGCGCAAC AGCATCTGTT    61 GCAACTCACA GTCTGGGGCA TCAAGCAGCT CCAGGCAAGA ATCCTGGCTG TGGAAAGATA   121 CCTAAAGGAT CAACAGCTCC TGGGGATTTG GGGTTGCTCT GGAAAACTCA TTTGCACCAC   181 TGCTGTGCCT TGGAATGCTA GTTGGAGTAA TAAATCTCTG GAACAGATTT GGAATCACAC   241 GACCTGGATG GAGTGGGACA GAGAAATTAA CAATTACACA AGCTTAATAC ACTCCTTAAT   301 TGAAGAATCG CAAAACCAGC AAGAAAAGAA TGAACAAGAA TTATTGGAAT TAGATAAATG   361 GGCAAGTTTG TGGAATTGGT TTAACATAAC AAATTGGCTG TGGTATATAA AATTATTCAT   421 AATGATAGTA GGAGGCTTGG TAGGTTTAAG AATAGTTTTT GCTGTACTTT CTATAGTGAA   481 TAGAGTTAGG CAGGGATATT CACCATTATC GTTTCAGACC CACCTCCCAA CCCCGAGGGG   541 ACCCGACAGG CCCGAANGAA TAGAAGAAGA ANGTGGAGAG AGAGACAGAN ACAGATCCNT   601 TCGATTAGTG AACGGNTCTA CNAATGGCAG TATTCNTCCN CAATNNNAAA AGAAAAGGGG   661 GGNNTGGGGG GGNACAGNGN NNNNNNGNAN AGTANANNTN NNNNCNNNNG NNNNNNNANN 721 NNNNNNNTNN NNAANNNANN NNNNNNNN //
Primer: bGH_rev3 Region: bGHpA, 3' LTR, loxP, PRE Sequence file: RDB12882_B0Jke.seq
>12882_12882_B0Jk_bGH_rev3_F08_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN GNNGGGCAAA CAACAGATGG CTGGCAACTA GAAGGCACAG    61 TCGAGGCTGA TCAGCGGGTT TAAACGGGCC CTGCTAGAGA TTTTCCACAC TGACTAAAAG   121 GGTCTGAGGG ATCTCTAGTT ACCAGAGTCA CACAACAGAC GGGCACACAC TACTTGAAGC   181 ACTCAAGGCA AGCTTTATTG AGGCTTAAGC AGTGGGTTCC CTAGTTAGCC AGAGAGCTCC   241 CAGGCTCAGA TCTGGTCTAA CCAGAGAGAC CCAGTACAGT CCGGAATAAC TTCGTATAAT   301 GTATGCTATA CGAAGTTATG ATATCTTGTC TTCGTTGGGA GTGAATTAGC CCTTCCAGTC   361 CCCCCTTTTC TTTTAAAAAG TGGCTAAGAT CTACAGCTGC CTTGTAAGTC ATTGGTCTTA   421 AAGGTACCTG AGGTGTGACT GGAAAACCCA CCTCCTCCTC CTCTTGTGCT TCTAGCCAGG   481 CACAATCAGC ATTGGTAGCT GCTGTATTGC TACTTGTGAT TGCTCCATGT TTTTCTAGGT   541 CTCGACGGTA TCGATGCGGG GAGGCGGCCC AAAGGGAGAT CCGACTCGTC TGANGGCGAA   601 NGCGAAGANG CGGAANANGN CGCAGANCCG GCAGCANGNC GCGGGAANGA ANGNNCGCTG 661 GNNTGAGGGN CNAANNGNAC GNANNNNNNN NNGTCCNNCG NNNANTNNNN NNNNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, EM7 pro, Zeo Sequence file: RDB12882_B0Jkf.seq
>12882_12882_B0Jk_SV40pro_ori_F_G08_20_ABI24.ab1     1 NNNNNNNNNN NNGNCGCCTC TGCCTCTGAN CTATTCCNGA AGTAGTGAGG AGGCTTTTTT    61 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT   121 CAGCACGTGT TGACAATTAA TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG   181 TGAGGAACTA AACCATGGCC AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG   241 TCGCCGGAGC GGTCGAGTTC TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG   301 ACGACTTCGC CGGTGTGGTC CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC   361 AGGTGGTGCC GGACAACACC CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG   421 CCGAGTGGTC GGAGGTCGTG TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG   481 AGATCGGCGA GCAGCCGTGG GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG   541 TGCACTTCGT GGCCGANGAN CANGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG   601 CCTTCTATGA AAGNNTGGGC TTCNGANTCG TTTTCCGGGA NGCCNGCTGG ATGANNCTCC 661 NGCGCGGGGN NCTCNTGCTG NANTNCNTCG CCNNCCCNNN NNNNNTNNNN NNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.08.12

GNP_filter3_RDBDEP_html_220812.pl