Resource data sheet
Prev. |  Lentiviral vector plasmid > 
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CSIV-TRE-GFP-UbC-KT

Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by UbC promoter.

Catalog number RDB12877
Resource name CSIV-TRE-GFP-UbC-KT
Clone info. Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by UbC promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSIV-TRE-RfA-UbC-KT ()
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database

            Reference sequence
              
             
            Sequence (full) RDB12877zzk02.seq provided by the depositor
            Remarks, protocol and/or map (pdf) RDB12877.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            MTA, for use for not-for-profit academic purpose [Word]
            Please visit Ordering instruction.[link] 
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            Please contact us. We will provide you with appropriate documents.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利学術目的用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB12877 CSIV-TRE-GFP-UbC-KT DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CSIV-TRE-GFP-UbC-KT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12877).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Lentiviral plasmid list (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12877_A5D2p1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EGFP-N (Pr0027)
            Region: attB1, tight TRE pro, cPPT_CTS
            Sequence file: RDB12877_A5D2h.seq check
            >12877_12877_A5D2_2_EGFP-N_A08_02.ab1
                1 NNNNNNNNNN NNNNNCNNNN CNCNNTTGCT NNNNNTGGTG GNGCCTGCTT TTTTGTACNA
               61 ACTTGTGATG CTAGCGGCGA TCTGACGGTT CACTAAACGA GCTCTGCTTA TATAGGCCTC
              121 CCACCGTACA CGCCTACCTC GACATACGTT CTCTATCACT GATAGGGAGT AAACTCGACA
              181 TACGTTCTCT ATCACTGATA GGGATAAACT CGACATACGT TCTCTATCAC TGATAGGGAG
              241 TAAACTCGAC ATACGTTCTC TATCACTGAT AGGGAGTAAA CTCGACATAC GTTCTCTATC
              301 ACTGATAGGG AGTAAACTCG ACATCGTTCT CTATCACTGA TAGGGAGTAA ACTCGACATA
              361 CGTTCTCTAT CACTGATAGG GAGTAAACTC GATTGAAGAC GAAAGGGCCG AATTCAAAGT
              421 GAATTTCTGC TGTCCCTGTA ATAAACCCGA AAATTTTGAA TTTTTGTAAT TTGTTTTTGT
              481 AATTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA TTCTTTCCCC TGCACTGTAC
              541 CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA CTGCCATTTG TAGATCCGTT
              601 CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT TCTTCTTCTA TTCCTTCGGG
              661 CCTGTCGGGT CCNNTCGGGG TTGGGAGGTG GGTCTGAAAC GATAATGGTG AATATCCCTG
              721 CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT CTTAAACCTA CCAAGCCTCC
              781 TACTATCATT ATGAATAATT TTATATACCA CAGCCNATTT GTTATGTTAA ACCNATTCCA
              841 CAAACTTGCC NATTTATCTA ATTNCAATAA TTCTTGTTCA TTCTTTTCTT GCTGGTTTTG
              901 CGATTCTNCN ATTAANGANT GNATTAAGCT TGTGTANNTG NTAANTTCTN NGNCCNNCTN
            961 CATCCNNGNC NNGNGATTNC NAATCNGNNN CANANNNTNN NNANNNCNNN NNNNNNN
            //
            Primer: mVenus-M_F (Pr0574)
            Region: EGFP 3', attB2, UbC pro
            Sequence file: RDB12877_A5D2i.seq check
            >12887_12887_B1HkF_mVenus-M_F_F01_16_ABI24.ab1
                1 NNNNNNNNNN NNNGNNTNNN GGTGNNCTTC NAGATCCGCC ACAACATCGA GGNCGGCAGC
               61 GTGCAGCTCG CCGACCACTA CCAGCAGAAC ACCCCCATCG GCGACGGCCC CGTGCTGCTG
              121 CCCGACAACC ACTACCTGAG CACCCAGTCC GCCCTGAGCA AAGACCCCAA CGAGAAGCGC
              181 GATCACATGG TCCTGCTGGA GTTCGTGACC GCCGCCGGGA TCACTCTCGG CATGGACGAG
              241 CTGTACAAGT CCGGACTCAG ATCCTACTAG TAGGATCTCG AGATATCTAG ACCCAGCTTT
              301 CTTGTACAAA GTGGTGATGC TACCGGTCTC GAGATCGGGA GATCTGGCCT CCGCGCCGGG
              361 TTTTGGCGCC CCCCGCGGGC GCCCCCCTCC TCACGGCGAG CGCTGCCACG TCAGACGAAG
              421 GGCGCAGCGA GCGTCCTGAT CCTTCCGCCC GGACGCTCAG GACAGCGGCC CGCTGCTCAT
              481 AAGACTCGGC CTTAGAACCC CAGTATCAGC AGAAGGACAT TTTAGGACGG GACTTGGGTG
              541 ACTCTAGGGC ACTGGTTTTC TTTCCAGAGA GCGGAACAGG CGAGGAAAAG TAGTCCCTTC
              601 TCGGCGANTC TGCGGANGGA TCTCCGTGGG GCGGTGAACG CCGATGATTA TANNANGACG
            661 CGCCNGGGTG TGGCACAGCT AGTTCCGTCN CANNNNGGNN TTGGGTCNNN NNNNNNN
            //
            Primer: UbCpro_F (Pr0158)
            Region: UbC pro, hKO 5'
            Sequence file: RDB12877_A5D2j.seq check
            >12877_12877_A5D2_2_UbCpro_F_C08_08.ab1
                1 NNNNNNNNNN NNNGNNTNNG NNNNGTAANT TGTCCGCTNA ATTCTGGCCG TTTTTGGCTT
               61 TTTTGTTAGA CCGGACCGTG TTTAGCGCTA CCGGTCCACC ATGGTGAGCG TGATCAAGCC
              121 CGAGATGAAG ATGAAGTACT TCATGGACGG CAGCGTGAAC GGCCACGAGT TCACCGTGGA
              181 GGGCGAGGGC ACCGGCAAGC CCTACGAGGG CCACCAGGAG ATGACCCTGA GGGTGACAAT
              241 GGCCAAGGGC GGCCCCATGC CCTTCAGCTT CGACCTGGTG AGCCACACCT TCTGCTACGG
              301 CCACAGGCCC TTCACCAAGT ACCCCGAGGA GATCCCCGAC TACTTCAAGC AGGCCTTCCC
              361 CGAGGGCCTG AGCTGGGAGA GGAGCCTCCA GTTCGAGGAC GGCGGCTTCG CCGCCGTGAG
              421 CGCCCACATC AGCCTGAGGG GCAACTGCTT CGAGCACAAG AGCAAGTTCG TGGGCGTGAA
              481 CTTCCCCGCC GACGGCCCCG TGATGCAGAA CCAGAGCAGC GACTGGGAGC CCAGCACCGA
              541 GAAGATCACC ACCTGCGACG GCGTGCTGAA GGGCGACGTG ACCATGTACC TGAAGCTGGC
              601 CGGCGGCGGC AACCACAAGT GCCAGTTCAA GACCACCTAC AAGGCCGCCA AGAAGATCCT
              661 GAAGATGCCC CAGAGCCACT TCATCGGCCA CAGGCTGGTG ANGAAGANCG AGGGCAACAT
              721 CACCGAGCTG GTGGAGGANG CCGTGGCCCA CTGCGAGGGC AGANGAAGTC TTCTAACATG
              781 CGGTGACGTG GANGAGAATC CCGGCCNTAT GTCTAGACTG GACNAGANCA AAGTCATAAA
              841 CGGCGCTCTG GAATTACTCA ATGGAGTCGG TATCGAANGN CTGACGANNA NGAAACTCGC
              901 TCAAAAGCTG GGANTTGANC AGCCNANCNN GNACTNGNNC NNNAANAANN AGCGGGNCNN
            961 GNNCGANGCC CNGNCNATCN NNANGNNNGN NNNNNNNNN
            //
            Primer: mKO2-M_F (Pr0578)
            Region: hKO 5', T2A, rtTA-Advanced
            Sequence file: RDB12877_A5D2k.seq check
            >12877_12877_B1HkF_mKO2-M_F_H01_22_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNC NNNNTNNNGN NCNCCTACAA GGCCGCCAAG AAGNTCCTGA
               61 AGATGCCCCA GAGCCACTTC ATCGGCCACA GGCTGGTGAG GAAGACCGAG GGCAACATCA
              121 CCGAGCTGGT GGAGGACGCC GTGGCCCACT GCGAGGGCAG AGGAAGTCTT CTAACATGCG
              181 GTGACGTGGA GGAGAATCCC GGCCCTATGT CTAGACTGGA CAAGAGCAAA GTCATAAACG
              241 GCGCTCTGGA ATTACTCAAT GGAGTCGGTA TCGAAGGCCT GACGACAAGG AAACTCGCTC
              301 AAAAGCTGGG AGTTGAGCAG CCTACCCTGT ACTGGCACGT GAAGAACAAG CGGGCCCTGC
              361 TCGATGCCCT GCCAATCGAG ATGCTGGACA GGCATCATAC CCACTTCTGC CCCCTGGAAG
              421 GCGAGTCATG GCAAGACTTT CTGCGGAACA ACGCCAAGTC ATTCCGCTGT GCTCTCCTCT
              481 CACATCGCGA CGGGGCTAAA GTGCATCTCG GCACCCGCCC AACAGAGAAA CAGTACGAAA
              541 CCCTGGAAAA TCAGCTCGCG TTCCTGTGTC AGCAAGGCTT CTCCCTGGAG AACGCACTGT
              601 ACGCTCTGTC CGCCGTGGGC CACTTTACAC TGGGCNGCGT ATTGGANGAA CAGGAGCATC
              661 NAGTAGCAAA AGANGNNNAG AGACACCTAC CACCNNTTCT ATGCCCCNNC TTCNGANANN
            721 NGCANNTNNN NNGNNNNNNN NGNNNNGGNN NNNNNNANNN NNNN
            //
            Primer: CMV_Forward (Pr0016)
            Region: CMV pro, 5' LTR, HIV-1 psi
            Sequence file: RDB12877_A5D2l.seq check
            >12877_12877_A5D2_2_CMV-Forward_E08_14.ab1
                1 NNNNNNNNNN NNNNNNNTGN NNNNNNGGGC GGTAGGCGTG TACGGTGGGA GGTCTATATA
               61 AGCAGCGCGT TTTGCCTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG CCTGGGAGCT
              121 CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT GAGTGCTTCA
              181 AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA GACCCTTTTA
              241 GTCAGTGTGG AAAATCTCTA GCAGTGGCGC CCGAACAGGG ACTTGAAAGC GAAAGGGAAA
              301 CCAGAGGAGC TCTCTCGACG CAGGACTCGG CTTGCTGAAG CGCGCACGGC AAGAGGCGAG
              361 GGGCGGCGAC TGGTGAGTAC GCCAAAAATT TTGACTAGCG GAGGCTAGAA GGAGAGAGAT
              421 GGGTGCGAGA GCGTCAGTAT TAAGCGGGGG AGAATTAGAT CGCGATGGGA AAAAATTCGG
              481 TTAAGGCCAG GGGGAAAGAA AAAATATAAA TTAAAACATA TAGTATGGGC AAGCAGGGAG
              541 CTAGAACGAT TCGCAGTTAA TCCTGGCCTG TTAGAAACAT CAGAAGGCTG TAGACAAATA
              601 CTGGGACAGC TACAACCATC CCTTCAGACA GGATCAGAAG AACTTAGATC ATTATATAAT
              661 ACAGTAGCAA CCCTCTATTG TGTGCATCAA AGGATAGAGA TAAAAGACAC CAAGGAAGCT
              721 TTAGACAAGA TAGAGGAAGA GCAAAACAAA AGTAAGACCA CCGCACAGCA AGCGGCCGCT
              781 GATCTTCAGA CCTGGAGGAG GAGATATGAN GGANAATTGG AGAAGTGAAT TATATAAATA
              841 TAAAGTAGTA AAAATTGAAC CATTANGAGT AGCACCCNCC NANGCAAAGA GAAGAGNGGT
              901 GCAGAGAGAA AAAAGANCAG TGGGAATANG AGCTTNGTTC CTNGGGNNCT NNGGANCAGC
            961 NNNAANCACT ATGGNNNCNG CNNNNNTGNN GNNNNNNNNN CNNGNNNNNN NNNN
            //
            Primer: EGFP-M_R (Pr0743)
            Region: EGFP 5', attB1, tight TRE pro
            Sequence file: RDB12877_A5D2m.seq check
            >12877_12877_B1HkF_EGFP-M_R_G01_19_ABI24.ab1
                1 NNNNNNNNNN NNNNNNGCGG NNCTNNNNNT NNCCTTGATG CCGTTCTTCT GCTTGTCGGC
               61 CATGATATAG ACGTTGTGGC TGTTGTAGTT GTACTCCAGC TTGTGCCCCA GGATGTTGCC
              121 GTCCTCCTTG AAGTCGATGC CCTTCAGCTC GATGCGGTTC ACCAGGGTGT CGCCCTCGAA
              181 CTTCACCTCG GCGCGGGTCT TGTAGTTGCC GTCGTCCTTG AAGAAGATGG TGCGCTCCTG
              241 GACGTAGCCT TCGGGCATGG CGGACTTGAA GAAGTCGTGC TGCTTCATGT GGTCGGGGTA
              301 GCGGCTGAAG CACTGCACGC CGTAGGTCAG GGTGGTCACG AGGGTGGGCC AGGGCACGGG
              361 CAGCTTGCCG GTGGTGCAGA TGAACTTCAG GGTCAGCTTG CCGTAGGTGG CATCGCCCTC
              421 GCCCTCGCCG GACACGCTGA ACTTGTGGCC GTTTACGTCG CCGTCCAGCT CGACCAGGAT
              481 GGGCACCACC CCGGTGAACA GCTCCTCGCC CTTGCTCACC ATGGTGGAGC CTGCTTTTTT
              541 GTACAAACTT GTGATGCTAG CGGCGATCTG ACGGTTCACT AAACGAGCTC TGCTTATATA
              601 GGCCTCCCAC CGTACACGCC TACCTCGACA TACGTTCTCT ATCACTGATA NGGAGTAAAC
              661 TCGACATACG TTCTCTATCN CTGANANGGN NAAACTCGAC ATACGTTCTC TATCNNNGNT
            721 NNGGNNTAAA NTNNNNNTAC NNTNNTCNNN NNNNNNN
            //
            Primer: PRE_rev (Pr0250)
            Region: PRE, rtTA-Advanced 3'
            Sequence file: RDB12877_A5D2n.seq check
            >12877_12877_B1HkF_PRE_rev_E01_05_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNCCN NTNAATCTTT NNCAAATTTT GTAATCCAGA GGTTGATTAT
               61 CGATAAGCTT GATCCCTCGA TGTTAACTCT AGAGGATCCT TACTTAGTTA CCCGGGGAGC
              121 ATGTCAAGGT CAAAATCGTC AAGAGCGTCA GCAGGCAGCA TATCAAGGTC AAAGTCGTCA
              181 AGGGCATCGG CTGGGAGCAT GTCTAAGTCA AAATCGTCAA GGGCGTCGGC CGGCCCGCCG
              241 CTTTCGCACT TTAGCTGTTT CTCCAGGCCA CATATGATTA GTTCCAGGCC GAAAAGGAAG
              301 GCAGGTTCGG CTCCCTGCCG GTCGAACAGC TCAATTGCTT GTCTCAGAAG TGGGGGCATA
              361 GAATCGGTGG TAGGTGTCTC TCTTTCCTCT TTTGCTACTT GATGCTCCTG TTCCTCCAAT
              421 ACGCAGCCCA GTGTAAAGTG GCCCACGGCG GACAGAGCGT ACAGTGCGTT CTCCNNGGAG
              481 AAGCCTTGCT GACNCAGGAA NNNNANCTGA TTTTCCAGGG TTNCNNACTG TTTCTCTGTT
              541 GNNNNNNTGN NNNNATGCAC TTTANNCCCG NCNNNANNNG ANANNANAGN ACAGNGNANN
            601 GANTTGNNNN NNNNCNNNNN NNNNN
            //
            Primer: bGH_rev3 (Pr0885)
            Region: bGH pA, 3'LTR, loxP, PRE
            Sequence file: RDB12877_A5D2o.seq check
            >12877_12877_B1HkF_bGH_rev3_H03_24_ABI24.ab1
                1 NNNNNNNNNN NNNNNGNNNN NCAACTAGAA GGCACAGTCG AGGCTGATCA GCGGGTTTAA
               61 ACGGGCCCTG CTAGAGATTT TCCACACTGA CTAAAAGGGT CTGAGGGATC TCTAGTTACC
              121 AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT CAAGGCAAGC TTTATTGAGG
              181 CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG GCTCAGATCT GGTCTAACCA
              241 GAGAGACCCA GTACAGTCCG GAATAACTTC GTATAATGTA TGCTATACGA AGTTATGATA
              301 TCTTGTCTTC GTTGGGAGTG AATTAGCCCT TCCAGTCCCC CCTTTTCTTT TAAAAAGTGG
              361 CTAAGATCTA CAGCTGCCTT GTAAGTCATT GGTCTTAAAG GTACCTGAGG TGTGACTGGA
              421 AAACCCACCT CCTCCTCCTC TTGTGCTTCT AGCCAGGCAC AATCAGCATT GGTAGCTGCT
              481 GTATTGCTAC TTGTGATTGC TCCATGTTTT TCTAGGTCTC GACGGTATCG ATGCGGGGAN
              541 GCGGCCCAAA GGGANNTCCG ACTCGTCTGA NGGCGAAGGC GAANNNGCNG AAGANGCCGC
              601 ANAGCCGGNN NNNNGCCGCN GGAANGAANG NCCNCTGGNN TGAGGGNCNN ANGGNANGTA
            661 NNNNANNNAC GTCCCGCNNN NANTCNNNNN NNNNN
            //
            Primer: SV40pro_F (Pr0110)
            Region: Zeo, SV40 pA
            Sequence file: RDB12877_A5D2p.seq check
            >12877_12877_A5D2_2_SV40pro_F_G08_20.ab1
                1 NNNNNNNNNN NNNNNNNNNN NANNNNNGNN GANGNTTTTT TGGAGGNCTA GGCTTTTGCN
               61 AAAAGCTCCC GGGAGCTTGT ATATCNNTTT TCGGATCTGA TCAGCACGTG TTGACAATTA
              121 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
              181 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT
              241 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT
              301 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC
              361 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT
              421 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG
              481 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA
              541 GCAGGACTGA CACGTGCTAC GAGATTTCGA TTCCACCGCC GCCTTCTATG AAAGGTTGGG
              601 CTTCGGAATC GTTTTCCGGG ACGCCGGCTG GATGATCCTC CAGCGCGGGG ATCTCATGCT
              661 GGAGTTCTTC GCCCACCCCA ACTTGTTTAT TGCAGCTTAT AATGGTTACA AATAAAGCAA
              721 TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG CATTCTAGTT GTGGTTTGTC
              781 CNAACTCATC AATGTATCTT ATCATGTCTG TATACCGTCG ACCTCTAGCT AGAGCTTGNC
              841 GTAATCATGG TCATAGCTGT TTCCTGTGTG NAATTGTTAT CCGCTCACAA TTCCNCACAA
              901 CATACGANCC GGAAGCATAA AGTGTAAAGN CTGGGGTGNC TAATGANTGA GCTAACTCAC
              961 ATTAATTGCG NNGCGCTCNC TGNCNNNNTT NCAGNCNGNA AACCNGNCGN GNNNNNNNNN
            1021 N
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2023.11.16

            GNP_filter3_RDBDEP_html_231027.pl