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CSIV-GFP-TRE-EF-KT

Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by EF-1 alpha promoter.

Catalog number RDB12871
Resource name CSIV-GFP-TRE-EF-KT
Clone info. Lentivirus vector plasmid of GFP driven by Tet-responsive promoter and hKO1-2A-rtTA driven by EF-1 alpha promoter. Please note that the TRE and EF promoters are placed in back-to-back orientation and this construct contains loxP.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Vector backbone CSIV-RfA-TRE-EF-KT (Plasmid)
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
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External Database

            Reference sequence
              
             
            Sequence (full) RDB12871zzt02.seq compiled by us

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
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            Remarks Remember that you will be working with samples containing infectious virus.
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            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
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            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

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            RDB12871 CSIV-GFP-TRE-EF-KT DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CSIV-GFP-TRE-EF-KT was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12871).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


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            Featured content

            Featured content Lentiviral plasmid list (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB19477_B2Abp1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: VN_f1 (Pr0992)
            Region: EGFP 5',attB2,cPPT_CTS
            Sequence file: RDB19477_B2Aba.seq check
            >12871_19477_B2Ab_2_VN_F1_F01_06_ABI08.ab1
                1 GGCCGACAAG CAGAAGAACG GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA
               61 CGGCAGCGTG CAGCTCGCCG ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT
              121 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA
              181 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT
              241 GGACGAGCTG TACAAGTCCG GACTCAGATC CTACTAGTAG GATCTCGAGA TATCTAGACC
              301 CAGCTTTCTT GTACAAAGTG GTGATGCTAC CGGAGAATTC AAAGTGAATT TCTGCTGTCC
              361 CTGTAATAAA CCCGAAAATT TTGAATTTTT GTAATTTGTT TTTGTAATTC TTTAGTTTGT
              421 ATGTCTGTTG CTATTATGTC TACTATTCTT TCCCCTGCAC TGTACCCCNN NNNNNCCCCT
              481 TTTCTTTTAA AATTGTGGAT GAATACTGCC ATTTGTAGAT CCGTTCACTA ATCGAATGGA
              541 TCTGTCTCTG TCTCTCTCTC CACCTTCTTC TTCTATTCCT TCNGGCCTGT CGGGTCCCCT
              601 CGGGGTTGGG AGGTGGGTCT GAAAACNANN ANTGGTGAAT ATCCNTGNCN NNACTCTATT
            661 NNNCNNTANA AANGTACNNG NNNAAAAACT NANTTNNNNN NNNCCNNNNC NNNNNN
            //
            Primer: EF1a-N_R (Pr0024)
            Region: EF-1 alpha pro,tight TRE pro,attB1,EGFP 5'
            Sequence file: RDB19477_B2Abb.seq check
            >12871_19477_B2Ab_2_EF1a_N_R_B03_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGCGNNGTG CGCTCTGCCC NCTGACGGGC ACCGGAGCCT
               61 CACGAAGCTT GAATTCGGCC CTTTCGTCTT CAATCGAGTT TACTCCCTAT CAGTGATAGA
              121 GAACGTATGT CGAGTTTACT CCCTATCAGT GATAGAGAAC GATGTCGAGT TTACTCCCTA
              181 TCAGTGATAG AGAACGTATG TCGAGTTTAC TCCCTATCAG TGATAGAGAA CGTATGTCGA
              241 GTTTACTCCC TATCAGTGAT AGAGAACGTA TGTCGAGTTT ATCCCTATCA GTGATAGAGA
              301 ACGTATGTCG AGTTTACTCC CTATCAGTGA TAGAGAACGT ATGTCGAGGT AGGCGTGTAC
              361 GGTGGGAGGC CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGATCGCC GCTAGCATCA
              421 CAAGTTTGTA CAAAAAAGCA GGCTCCACCA TGGTGAGCAA GGGCGAGGAG CTGTTCNCCG
              481 GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA CGGCCACAAG TTCNGCGTGT
              541 CCGGCGAGGG CGAGGGCGAT GCCNCCTACN GCAAGCTGAN CCTGAAGTTN NTCTGCACCA
              601 CCGGCNAGCT GCCCGNGCCC NNNNCCNCCC TCNNGACCNN CCTGACCTAC NGNGNGNANN
            661 GNNTCNNCCG CTACCCNNAN CNCNTGAAGN NNNNNNANTT CNTCANNCNN NNNNNNNN
            //
            Primer: EF1a-C_F (Pr0168)
            Region: EF-1 alpha pro,hKO 5'
            Sequence file: RDB19477_B2Abc.seq check
            >12871_19477_B2Ab_2_EF1a-C_F_B02_05_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN TNAGTTTGGN NCTTGGTTNN TTCTCNANGC CTNNNACAGT
               61 GGTTCAAAGT TTTTTTCTTC CATTTCAGGT GTCGTGAACA CGCTACCGGT CCACCATGGT
              121 GAGCGTGATC AAGCCCGAGA TGAAGATGAA GTACTTCATG GACGGCAGCG TGAACGGCCA
              181 CGAGTTCACC GTGGAGGGCG AGGGCACCGG CAAGCCCTAC GAGGGCCACC AGGAGATGAC
              241 CCTGAGGGTG ACAATGGCCA AGGGCGGCCC CATGCCCTTC AGCTTCGACC TGGTGAGCCA
              301 CACCTTCTGC TACGGCCACA GGCCCTTCAC CAAGTACCCC GAGGAGATCC CCGACTACTT
              361 CAAGCAGGCC TTCCCCGAGG GCCTGAGCTG GGAGAGGAGC CTCCAGTTCG AGGACGGCGG
              421 CTTCGCCGCC GTGAGCGCCC ACATCAGCCT GAGGGGCAAC TGCTTCGAGC ACAAGAGCAA
              481 GTTCGTGGGC GTGAACTTCC CCGCCGACGG CCCCGTGATG CAGAACCNGA GCAGCGACTG
              541 GGAGCCCAGC ACCNAGAAGA TCACCNCCTG CNACNGCGTG CTGAAGGGCG ANGTGACCAT
              601 GTACCTGAAN NTGGNCGGNG GNNGCANCCA CNAGTGCNNN TTCNANANCN NCTACNANNN
              661 NNNCAANAAN ANNCTGAANA NGNCCNNNAN CCNCTTCNTC NGNNNNNNGN NGGNNNNGAN
            721 NANNNNGGGN NNNNNNCCNA NCNGNGNNNN NNNNNN
            //
            Primer: PRE_rev (Pr0250)
            Region: PRE,rt TA Advanced
            Sequence file: RDB19477_B2Abd.seq check
            >12871_19477_B2Ab_2_PRE_rev_D02_11_ABI24.ab1
                1 NNNNNNNNNN NNNNNGNNNA AGGAGCANNN TAGTTAAGAA TACCAGTCAA TCTTTCNCAA
               61 ATTTTGTAAT CCAGAGGTTG ATTATCGATA AGCTTGATCC CTCGATGTTA ACTCTAGAGG
              121 ATCCTTACTT AGTTACCCGG GGAGCATGTC AAGGTCAAAA TCGTCAAGAG CGTCAGCAGG
              181 CAGCATATCA AGGTCAAAGT CGTCAAGGGC ATCGGCTGGG AGCATGTCTA AGTCAAAATC
              241 GTCAAGGGCG TCGGCCGGCC CGCCGCTTTC GCACTTTAGC TGTTTCTCCA GGCCACATAT
              301 GATTAGTTCC AGGCCGAAAA GGAAGGCAGG TTCGGCTCCC TGCCGGTCGA ACAGCTCAAT
              361 TGCTTGTCTC AGAAGTGGGG GCATAGAATC GGTGGTAGGT GTCTCTCTTT CCTCTTTTGC
              421 TACTTGATGC TCCTGTTCCT CCAATACGCA GCCCAGTGTA AAGTGGCCCA CGGCGGACAG
              481 AGCGTACAGT GCGTTCTCCA GGGAGAAGCC TTGCTGACAC AGGAACGCGA GCTGATTTTC
              541 CNGGGTTTCG TACTGTTTCT CTGTTGGGCG GGTGCCGAGA TGCACTTTAG CCCCGTCNCG
              601 ATGTGAGAGG ANAGCACNGC NNAATGACNT GGCGTTGNTC CNNNNAAANN CNTGNCNNGA
              661 CTCNNNNNNC NNGGGGNNNA ANNGGNNANG ANGNCNGNNC NNNNTCTCNA NNNNNNNNNT
            721 CNNNNNNNNN N
            //
            Primer: CMV_Forward (Pr0016)
            Region: CMV pro,5' LTR,HIV-1 psi
            Sequence file: RDB19477_B2Abe.seq check
            >12871_19477_B2Ab_2_CMV_Forward_F06_06_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN TTGNNNCNNN GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
               61 TATAAGCAGC GCGTTTTGCC TGTACTGGGT CTCTCTGGTT AGACCAGATC TGAGCCTGGG
              121 AGCTCTCTGG CTAACTAGGG AACCCACTGC TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC
              181 TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG ACTCTGGTAA CTAGAGATCC CTCAGACCCT
              241 TTTAGTCAGT GTGGAAAATC TCTAGCAGTG GCGCCCGAAC AGGGACTTGA AAGCGAAAGG
              301 GAAACCAGAG GAGCTCTCTC GACGCAGGAC TCGGCTTGCT GAAGCGCGCA CGGCAAGAGG
              361 CGAGGGGCGG CGACTGGTGA GTACGCCAAA AATTTTGACT AGCGGAGGCT AGAAGGAGAG
              421 AGATGGGTGC GAGAGCGTCA GTATTAAGCG GGGGAGAATT AGATCGCGAT GGGAAAAAAT
              481 TCGGTTAAGG CCAGGGGGAA AGAAAAAATA TAAATTAAAA CATATAGTAT GGGCAAGCAG
              541 GGAGCTAGAA CGATTCGCAG TTAATCCTGG CCTGTTAGAA ACATCAGAAG GCTGTAGACA
              601 AATACTGGGA CAGCTACAAC CATCCCTTCA GACAGGATCA GAAGAACTTA GATCATTATA
              661 TAATACAGTA GCAACCCTCT ATTGTGTGCA TCAAAGGATA GAGATAAAAG ACACCAAGGA
              721 AGCTTTAGAC AAGATAGAGG AAGAGCAAAA CAAAAGTAAG ACCACCGCAC AGCAAGCGGC
              781 CGCTGATCTT CAGANCTGGA GGANGAGATA TGAGGGACAA TTGGAGAAGT GAATTATATN
              841 AATATAAAGT AGTAAAAATT GAACCATTAN GAGTAGCACC CACCNAGGCA AANAGAAGAG
              901 TGGNTGCAGA NAGNAAAAAG ANCAGNGGGA ATANGANCTT TGTTCCNNGG GTNCTNGGGA
              961 GCAGCNNNAN NCACTATNGG CGCNNCGNNN NTGNNGCTNN NNNTNNNNNN NCNNNNNNNT
            1021 ATNGNNNNNN NNNN
            //
            Primer: RRE_F1 (Pr0796)
            Region: RRE
            Sequence file: RDB19477_B2Abf.seq check
            >12871_19477_B2Ab_2_RRE_F1_F02_17_ABI24.ab1
                1 NNNNNNNNNN NGNNNNNNNN NAANNATTTG CTGAGGGCTA TTGAGGCGCA ACAGCATCTG
               61 TTGCAACTCA CAGTCTGGGG CATCAAGCAG CTCCAGGCAA GAATCCTGGC TGTGGAAAGA
              121 TACCTAAAGG ATCAACAGCT CCTGGGGATT TGGGGTTGCT CTGGAAAACT CATTTGCACC
              181 ACTGCTGTGC CTTGGAATGC TAGTTGGAGT AATAAATCTC TGGAACAGAT TTGGAATCAC
              241 ACGACCTGGA TGGAGTGGGA CAGAGAAATT AACAATTACA CAAGCTTAAT ACACTCCTTA
              301 ATTGAAGAAT CGCAAAACCA GCAAGAAAAG AATGAACAAG AATTATTGGA ATTAGATAAA
              361 TGGGCAAGTT TGTGGAATTG GTTTAACATA ACAAATTGGC TGTGGTATAT AAAATTATTC
              421 ATAATGATAG TAGGAGGCTT GGTAGGTTTA AGAATAGTTT TTGCTGTACT TTCTATAGTG
              481 AATAGAGTTA GGCAGGGATA TTCACCATTA TCGTTTCNGA CCCACCTCCC AACCCCGAGG
              541 GGACCCGACA GGCCCGAANG AATAGAAGAA GAANGTGGAG AGAGAGACAG ANACNGATCC
              601 NTTCNANTAG TGNACGGATC TACNAATGGC AGTATTNNTC NNCNANTTTN AAANAAAANG
              661 GGGGNNTGGG GGGNANNGNG NNNGGNAAAG ANAGTNNANN TNATANCANN GANNTNCNAN
            721 CNNANNANTN NNAANNANTN NNANNNNNNT TNNNNNNNNN N
            //
            Primer: EGFP-M_R (Pr0743)
            Region: EGFP 5',attB1
            Sequence file: RDB19477_B2Abg.seq check
            >12871_19477_B2Ab_2_EGFP-M_R_A02_02_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNG TGGNGGNNCT TGNNGTTNNC CTTGATGCCG TTCTTCTGCT
               61 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
              121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC
              181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC
              241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT
              301 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG
              361 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT
              421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA
              481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GTGGAGCCTG
              541 CTTTTTTGTA CNAACTTGTG ATGCTAGCGG CGATCTGACG GTTCNCTAAA CGAGCTCTGC
              601 TNATATAGGC NTCCNANCGN ACACNNCTAC CTCNNCNTNC GTTCTCTATC ACTGATNGGG
              661 AGTAANCTCN ACNNNCNTNC TCTANNNCNG ANANGGANNA ANTCNANNTN NNTNNCNNTC
            721 NNNGANNNGN NNTNAANNNN NNNNNN
            //
            Primer: tTA-Ad R (Pr0542)
            Region: rt TA-Advanced 5',T2A,hKO 3'
            Sequence file: RDB19477_B2Abh.seq check
            >12871_19477_B2Ab_2_tTA-Ad R_C02_08_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNT NNNGANTTTG CTCTTGTCNN GTCTAGNNCN TAGGGCCGGG
               61 ATTCTCCTCC ACGTCACCGC ATGTTAGAAG ACTTCCTCTG CCCTCGCAGT GGGCCACGGC
              121 GTCCTCCACC AGCTCGGTGA TGTTGCCCTC GGTCTTCCTC ACCAGCCTGT GGCCGATGAA
              181 GTGGCTCTGG GGCATCTTCA GGATCTTCTT GGCGGCCTTG TAGGTGGTCT TGAACTGGCA
              241 CTTGTGGTTG CCGCCGCCGG CCAGCTTCAG GTACATGGTC ACGTCGCCCT TCAGCACGCC
              301 GTCGCAGGTG GTGATCTTCT CGGTGCTGGG CTCCCAGTCG CTGCTCTGGT TCTGCATCAC
              361 GGGGCCGTCG GCGGGGAAGT TCACGCCCAC GAACTTGCTC TTGTGCTCGA AGCAGTTGCC
              421 CCTCAGGCTG ATGTGGGCGC TCACGGCGGC GAAGCCGCCG TCCTCGAACT GGAGGCTCCT
              481 CTCCCAGCTC AGGCCCTCGG GGAAGGCCTG CTTGAAGTAG TCGGGGATCT CCTCGGGGTA
              541 CTTGGTGAAG GGCCTGTGGC CGTAGCANAN NGTGTGGCTC ACCNGGTCNA AGCTNNANNG
              601 GCATGGGGNC GCCCTNGGNN NTTGTNNCCC TNNNGGTCNT CTCCTGGNGN NCNNCNTNNG
              661 NCTTGNCNGN GCCNTCNCCC NCCNNGGNGN ANTNNNNNNN NTNNNGNTNN NTNCNTNANN
            721 NNNNNNNCNN NNNNNCNGNC NNGANNNNNN NNNN
            //
            Primer: BGH_rev3 (Pr0885)
            Region: bGH pA,3' LTR,lox P
            Sequence file: RDB19477_B2Abi.seq check
            >12871_19477_B2Ab_2_bGH_rev3_H02_23_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNGNN GGNNGNNNNN NNNACNGATG GCTGGCAACT AGAAGGCACA
               61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA
              121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG
              181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC
              241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGAATAA CTTCGTATAA
              301 TGTATGCTAT ACGAAGTTAT GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT
              361 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT
              421 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG
              481 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCNTG TTTTTCTAGG
              541 TCTCGACGGT ATCGATGCGG GGAGGCGGNC CNNAGGGAGA TCCGACTCGT CTGANGGCGA
              601 ANGCNAANAC GCNGAANANG NCNCNNANCC NGCAGCNNNC NCGGGAANGN NGNNCCNCTG
            661 GNNTGNNGGN NNANNGNANN TANNNNNNNN NN
            //
            Primer: SV40pro_ori_F (Pr0731)
            Region: SV40 pro_ori,Zeo
            Sequence file: RDB19477_B2Abj.seq check
            >12871_19477_B2Ab_2_SV40pro_ori_F_A03_03_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTG CCTCNNNNNN ATTCNNGAAG TAGTGAGGAG
               61 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
              121 GATCTGATCA GCACGTGTTG ACAATTAATC ATCGGCATAG TATATCGGCA TAGTATAATA
              181 CGACAAGGTG AGGAACTAAA CCATGGCCAA GTTGACCAGT GCCGTTCCGG TGCTCACCGC
              241 GCGCGACGTC GCCGGAGCGG TCGAGTTCTG GACCGACCGG CTCGGGTTCT CCCGGGACNT
              301 CNTGGAGGAC GACTTCGCCG GTGTGGTCCG GGACGACGTG ACCCTGTTCA TCAGCGCGGT
              361 CCAGGACCAG GTGGTGCCGG ACAACACCCT GGCCTGGGTG TGGGTGCGCG GCCTGNACGA
              421 GCTGTACNCC NAGTGGTCGG AGGTCGTGNN NNNNAACTTC CGGGACGCCT CCGGGCCNGN
              481 NNNGACCGAG ATCGGCNAGN NNCCGTGGGG GCGGGAGTTC GCCCTGCNCG ACCCGGCCGG
              541 CAACTGNNNG CACTTCGTGG NCNANGANCA NGANTGACNC NNNNNNNNAG ATTTCNATNC
              601 CNCCNNCNCC TTCTATGAAN NGNTNGGNCT TNNNAATCNN TNNNCNGNNN NNNNGNNGGN
              661 NGANNCNNCN NNNNGNNNAT NNNNNNNNGN NNNTNNNNNN CCNNCCNNNC NNNNNNANNN
            721 NNNNNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles


            2023.11.16

            GNP_filter3_RDBDEP_html_231027.pl